IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Xylose phosphorylation functions as a molecular switch to regulate proteoglycan biosynthesis. Xylose phosphorylation functions as a molecular switch to regulate proteoglycan biosynthesis. Proceedings of the National Academy of Sciences of the United States of America 2014 imex curation Wen J., Xiao J., Rahdar M., Choudhury BP., Cui J., Taylor GS., Esko JD., Dixon JE., Wen J., Xiao J., Rahdar M., Choudhury BP., Cui J., Taylor GS., Esko JD., Dixon JE. DCN, GPC1, SDC1, and SDC4 ORFs were subcloned into pCCF (with a C-terminal FLAG tag), AND a modified pDisplay expression plasmid encoding a C-terminal strep-tag;however in most instances it is not stated which plasmid was used. Dark Space no 45? cDNAs for Human Decorin (DCN), SCDC4/1 and B3GALT6 were obtained from DNASU. Using the Gene name as the search term on the DNASU website, only human cDNAs are found. jedixon@ucsd.edu 20-Dec-2016: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro phosphotransferase phosphotransferase assay phosphotransferase weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Wen J., Xiao J., Rahdar M., Choudhury BP., Cui J., Taylor GS., Esko JD., Dixon JE., Wen J., Xiao J., Rahdar M., Choudhury BP., Cui J., Taylor GS., Esko JD., Dixon JE. DCN, GPC1, SDC1, and SDC4 ORFs were subcloned into pCCF (with a C-terminal FLAG tag), AND a modified pDisplay expression plasmid encoding a C-terminal strep-tag;however in most instances it is not stated which plasmid was used. Dark Space no 45? cDNAs for Human Decorin (DCN), SCDC4/1 and B3GALT6 were obtained from DNASU. Using the Gene name as the search term on the DNASU website, only human cDNAs are found. jedixon@ucsd.edu Accepted 2016-OCT-18 AT 10:52 BST AT 10:52 BST by ORCHARD Proceedings of the National Academy of Sciences of the United States of America 2014 imex curation Xylose phosphorylation functions as a molecular switch to regulate proteoglycan biosynthesis. Xylose phosphorylation functions as a molecular switch to regulate proteoglycan biosynthesis. Proceedings of the National Academy of Sciences of the United States of America 2014 imex curation Wen J., Xiao J., Rahdar M., Choudhury BP., Cui J., Taylor GS., Esko JD., Dixon JE., Wen J., Xiao J., Rahdar M., Choudhury BP., Cui J., Taylor GS., Esko JD., Dixon JE. DCN, GPC1, SDC1, and SDC4 ORFs were subcloned into pCCF (with a C-terminal FLAG tag), AND a modified pDisplay expression plasmid encoding a C-terminal strep-tag;however in most instances it is not stated which plasmid was used. Dark Space no 45? cDNAs for Human Decorin (DCN), SCDC4/1 and B3GALT6 were obtained from DNASU. Using the Gene name as the search term on the DNASU website, only human cDNAs are found. jedixon@ucsd.edu 20-Dec-2016: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro enzymatic study enzymatic study tag visualisation tag visualisation Wen J., Xiao J., Rahdar M., Choudhury BP., Cui J., Taylor GS., Esko JD., Dixon JE., Wen J., Xiao J., Rahdar M., Choudhury BP., Cui J., Taylor GS., Esko JD., Dixon JE. DCN, GPC1, SDC1, and SDC4 ORFs were subcloned into pCCF (with a C-terminal FLAG tag), AND a modified pDisplay expression plasmid encoding a C-terminal strep-tag;however in most instances it is not stated which plasmid was used. Dark Space no 45? Galactosyl-transferase assay cDNAs for Human Decorin (DCN), SCDC4/1 and B3GALT6 were obtained from DNASU. Using the Gene name as the search term on the DNASU website, only human cDNAs are found. jedixon@ucsd.edu Accepted 2016-OCT-18 AT 10:52 BST AT 10:52 BST by ORCHARD Proceedings of the National Academy of Sciences of the United States of America 2014 imex curation Xylose phosphorylation functions as a molecular switch to regulate proteoglycan biosynthesis. Xylose phosphorylation functions as a molecular switch to regulate proteoglycan biosynthesis. Proceedings of the National Academy of Sciences of the United States of America 2014 imex curation Wen J., Xiao J., Rahdar M., Choudhury BP., Cui J., Taylor GS., Esko JD., Dixon JE., Wen J., Xiao J., Rahdar M., Choudhury BP., Cui J., Taylor GS., Esko JD., Dixon JE. DCN, GPC1, SDC1, and SDC4 ORFs were subcloned into pCCF (with a C-terminal FLAG tag), AND a modified pDisplay expression plasmid encoding a C-terminal strep-tag;however in most instances it is not stated which plasmid was used. Dark Space no 45? cDNAs for Human Decorin (DCN), SCDC4/1 and B3GALT6 were obtained from DNASU. Using the Gene name as the search term on the DNASU website, only human cDNAs are found. jedixon@ucsd.edu 20-Dec-2016: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro phosphotransferase phosphotransferase assay phosphotransferase ms participant Identification by mass spectrometry ms participant Wen J., Xiao J., Rahdar M., Choudhury BP., Cui J., Taylor GS., Esko JD., Dixon JE., Wen J., Xiao J., Rahdar M., Choudhury BP., Cui J., Taylor GS., Esko JD., Dixon JE. DCN, GPC1, SDC1, and SDC4 ORFs were subcloned into pCCF (with a C-terminal FLAG tag), AND a modified pDisplay expression plasmid encoding a C-terminal strep-tag;however in most instances it is not stated which plasmid was used. Dark Space no 45? cDNAs for Human Decorin (DCN), SCDC4/1 and B3GALT6 were obtained from DNASU. Using the Gene name as the search term on the DNASU website, only human cDNAs are found. jedixon@ucsd.edu Accepted 2016-OCT-18 AT 10:52 BST AT 10:52 BST by ORCHARD Proceedings of the National Academy of Sciences of the United States of America 2014 imex curation Xylose phosphorylation functions as a molecular switch to regulate proteoglycan biosynthesis. Xylose phosphorylation functions as a molecular switch to regulate proteoglycan biosynthesis. Proceedings of the National Academy of Sciences of the United States of America 2014 imex curation Wen J., Xiao J., Rahdar M., Choudhury BP., Cui J., Taylor GS., Esko JD., Dixon JE., Wen J., Xiao J., Rahdar M., Choudhury BP., Cui J., Taylor GS., Esko JD., Dixon JE. DCN, GPC1, SDC1, and SDC4 ORFs were subcloned into pCCF (with a C-terminal FLAG tag), AND a modified pDisplay expression plasmid encoding a C-terminal strep-tag;however in most instances it is not stated which plasmid was used. Dark Space no 45? cDNAs for Human Decorin (DCN), SCDC4/1 and B3GALT6 were obtained from DNASU. Using the Gene name as the search term on the DNASU website, only human cDNAs are found. jedixon@ucsd.edu 20-Dec-2016: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro phosphotransferase phosphotransferase assay phosphotransferase tag visualisation tag visualisation Wen J., Xiao J., Rahdar M., Choudhury BP., Cui J., Taylor GS., Esko JD., Dixon JE., Wen J., Xiao J., Rahdar M., Choudhury BP., Cui J., Taylor GS., Esko JD., Dixon JE. DCN, GPC1, SDC1, and SDC4 ORFs were subcloned into pCCF (with a C-terminal FLAG tag), AND a modified pDisplay expression plasmid encoding a C-terminal strep-tag;however in most instances it is not stated which plasmid was used. Dark Space no 45? cDNAs for Human Decorin (DCN), SCDC4/1 and B3GALT6 were obtained from DNASU. Using the Gene name as the search term on the DNASU website, only human cDNAs are found. jedixon@ucsd.edu Accepted 2016-OCT-18 AT 10:52 BST AT 10:52 BST by ORCHARD Proceedings of the National Academy of Sciences of the United States of America 2014 imex curation tetra-ben Tetra-Ben small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) gal-xyl-ben Gal-Xyl-Ben small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) b3gt6_human Beta-1,3-galactosyltransferase 6 B3GALT6 GAG GalTII Galactosyltransferase II Galactosylxylosylprotein 3-beta-galactosyltransferase UDP-Gal:betaGal beta 1,3-galactosyltransferase polypeptide 6 protein protein human Homo sapiens Human MKLLRRAWRRRAALGLGTLALCGAALLYLARCAAEPGDPRAMSGRSPPPPAPARAAAFLAVLVASAPRAAERRSVIRSTWLARRGAPGDVWARFAVGTAGLGAEERRALEREQARHGDLLLLPALRDAYENLTAKVLAMLAWLDEHVAFEFVLKADDDSFARLDALLAELRAREPARRRRLYWGFFSGRGRVKPGGRWREAAWQLCDYYLPYALGGGYVLSADLVHYLRLSRDYLRAWHSEDVSLGAWLAPVDVQREHDPRFDTEYRSRGCSNQYLVTHKQSLEDMLEKHATLAREGRLCKREVQLRLSYVYDWSAPPSQCCQRREGIP F4E2A219B1DC11F7 pgca_bovin Aggrecan core protein ACAN AGC1 Cartilage-specific proteoglycan core protein protein protein bovin Bos taurus (Bovine) Bovine MTTLLLVFVTLRVITAAISVEVSEPDNSLSVSIPEPSPLRVLLGSSLTIPCYFIDPMHPVTTAPSTAPLAPRIKWSRISKEKEVVLLVATEGRVRVNSAYQDKVTLPNYPAIPSDATLEIQNMRSNDSGILRCEVMHGIEDSQATLEVVVKGIVFHYRAISTRYTLDFDRAQRACLQNSAIIATPEQLQAAYEDGFHQCDAGWLADQTVRYPIHTPREGCYGDKDEFPGVRTYGIRDTNETYDVYCFAEEMEGEVFYATSPEKFTFQEAANECRRLGARLATTGQLYLAWQGGMDMCSAGWLADRSVRYPISKARPNCGGNLLGVRTVYLHANQTGYPDPSSRYDAICYTGEDFVDIPESFFGVGGEEDITIQTVTWPDVELPLPRNITEGEARGSVILTAKPDFEVSPTAPEPEEPFTFVPEVRATAFPEVENRTEEATRPWAFPRESTPGLGAPTAFTSEDLVVQVTLAPGAAEVPGQPRLPGGVVFHYRPGSSRYSLTFEEAKQACLRTGAIIASPEQLQAAYEAGYEQCDAGWLQDQTVRYPIVSPRTPCVGDKDSSPGVRTYGVRPPSETYDVYCYVDRLEGEVFFATRLEQFTFWEAQEFCESQNATLATTGQLYAAWSRGLDKCYAGWLADGSLRYPIVTPRPACGGDKPGVRTVYLYPNQTGLLDPLSRHHAFCFRGVSAAPSPEEEEGSAPTAGPDVEEWMVTQVGPGVAAVPIGEETTAIPGFTVEPENKTEWELAYTPAGTLPLPGIPPTWPPTGEATEEHTEGPSATEVPSASEKPFPSEEPFPPEEPFPSEKPFPPEELFPSEKPFPSEKPFPSEEPFPSEKPFPPEELFPSEKPIPSEEPFPSEEPFPSEKPFPPEEPFPSEKPIPSEEPFPSEKPFPSEEPFPSEEPSTLSAPVPSRTELPSSGEVSGVPEISGDFTGSGEISGHLDFSGQPSGESASGLPSEDLDSSGLTSTVGSGLPVESGLPSGEEERITWTSAPKVDRLPSGGEGPEVSGVEDISGLPSGGEVHLEISASGVEDISGLPSGGEVHLEISASGVEDLSRIPSGEGPEISASGVEDISGLPSGEEGHLEISASGVEDLSGIPSGEGPEVSASGVEDLIGLPSGEGPEVSASGVEDLSRLPSGEGPEVSASGVEDLSGLPSGEGPEVSVSGVEDLSRLPSGEGPEVSASGVEDLSRLPSGEGPEISVSGVEDISILPSGEGPEVSASGVEDLSVLPSGEGHLEISTSGVEDLSVLPSGEGHLETSSGVEDISRLPSGEGPEVSASGVEDLSVLPSGEDHLEISASGVEDLGVLPSGEDHLEISASGVEDISRLPSGEGPEVSASGVEDLSVLPSGEGHLEISASGVEDLSRLPSGGEDHLETSASGVGDLSGLPSGREGLEISASGAGDLSGLTSGKEDLTGSASGALDLGRIPSVTLGSGQAPEASGLPSGFSGEYSGVDLESGPSSGLPDFSGLPSGFPTVSLVDTTLVEVVTATTAGELEGRGTIDISGAGETSGLPFSELDISGGASGLSSGAELSGQASGSPDISGETSGLFGVSGQPSGFPDISGETSGLLEVSGQPSGFYGEISGVTELSGLASGQPEISGEASGILSGLGPPFGITDLSGEAPGIPDLSGQPSGLPEFSGTASGIPDLVSSAVSGSGESSGITFVDTSLVEVTPTTFKEEEGLGSVELSGLPSGELGVSGTSGLADVSGLSSGAIDSSGFTSQPPEFSGLPSGVTEVSGEASGAESGSSLPSGAYDSSGLPSGFPTVSFVDRTLVESVTQAPTAQEAGEGPSGILELSGAPSGAPDMSGDHLGSLDQSGLQSGLVEPSGEPASTPYFSGDFSGTTDVSGESSAATSTSGEASGLPEVTLITSELVEGVTEPTVSQELGQRPPVTYTPQLFESSGEASASGDVPRFPGSGVEVSSVPESSGETSAYPEAEVGASAAPEASGGASGSPNLSETTSTFHEADLEGTSGLGVSGSPSAFPEGPTEGLATPEVSGESTTAFDVSVEASGSPSATPLASGDRTDTSGDLSGHTSGLDIVISTTIPESEWTQQTQRPAEARLEIESSSPVHSGEESQTADTATSPTDASIPASAGGTDDSEATTTDIDECLSSPCLNGATCVDAIDSFTCLCLPSYQGDVCEIQKLCEEGWTKFQGHCYRHFPDRATWVDAESQCRKQQSHLSSIVTPEEQEFVNNNAQDYQWIGLNDKTIEGDFRWSDGHSLQFENWRPNQPDNFFATGEDCVVMIWHEKGEWNDVPCNYQLPFTCKKGTVACGEPPVVEHARIFGQKKDRYEINALVRYQCTEGFIQGHVPTIRCQPSGHWEEPRITCTDPATYKRRLQKRSSRPLRRSHPSTAH 6FF83763420C3D4C sdc4_human Syndecan-4 SDC4 Amphiglycan Ryudocan core protein protein protein human Homo sapiens Human MAPARLFALLLFFVGGVAESIRETEVIDPQDLLEGRYFSGALPDDEDVVGPGQESDDFELSGSGDLDDLEDSMIGPEVVHPLVPLDNHIPERAGSGSQVPTEPKKLEENEVIPKRISPVEESEDVSNKVSMSSTVQGSNIFERTEVLAALIVGGIVGILFAVFLILLLMYRMKKKDEGSYDLGKKPIYKKAPTNEFYA DIP protein P31431 original sequence version: 87 DIP protein P31431-1 original sequence version: 134 8229AA2733F77A10 xylk_human Glycosaminoglycan xylosylkinase FAM20B KIAA0475 Xylose kinase protein protein human Homo sapiens Human MKLKQRVVLLAILLVIFIFTKVFLIDNLDTSAANREDQRAFHRMMTGLRVELAPKLDHTLQSPWEIAAQWVVPREVYPEETPELGAVMHAMATKKIIKADVGYKGTQLKALLILEGGQKVVFKPKRYSRDHVVEGEPYAGYDRHNAEVAAFHLDRILGFHRAPLVVGRFVNLRTEIKPVATEQLLSTFLTVGNNTCFYGKCYYCRETEPACADGDIMEGSVTLWLPDVWPLQKHRHPWGRTYREGKLARWEYDESYCDAVKKTSPYDSGPRLLDIIDTAVFDYLIGNADRHHYESFQDDEGASMLILLDNAKSFGNPSLDERSILAPLYQCCIIRVSTWNRLNYLKNGVLKSALKSAMAHDPISPVLSDPHLDAVDQRLLSVLATVKQCTDQFGMDTVLVEDRMPLSHL 03E211C0AF37B766 sdc1_human Syndecan-1 SDC1 SDC protein protein human Homo sapiens Human MRRAALWLWLCALALSLQPALPQIVATNLPPEDQDGSGDDSDNFSGSGAGALQDITLSQQTPSTWKDTQLLTAIPTSPEPTGLEATAASTSTLPAGEGPKEGEAVVLPEVEPGLTAREQEATPRPRETTQLPTTHLASTTTATTAQEPATSHPHRDMQPGHHETSTPAGPSQADLHTPHTEDGGPSATERAAEDGASSQLPAAEGSGEQDFTFETSGENTAVVAVEPDRRNQSPVDQGATGASQGLLDRKEVLGGVIAGGLVGLIFAVCLVGFMLYRMKKKDEGSYSLEEPKQANGGAYQKPTKQEEFYA DIP protein P18827 original sequence version: 77 DIP protein P18827 original sequence version: 127 8E9EF4A57F5FDBD0 pgs2_human Decorin DCN SLRR1B Bone proteoglycan II PG-S2 PG40 protein protein human Homo sapiens Human MKATIILLLLAQVSWAGPFQQRGLFDFMLEDEASGIGPEVPDDRDFEPSLGPVCPFRCQCHLRVVQCSDLGLDKVPKDLPPDTTLLDLQNNKITEIKDGDFKNLKNLHALILVNNKISKVSPGAFTPLVKLERLYLSKNQLKELPEKMPKTLQELRAHENEITKVRKVTFNGLNQMIVIELGTNPLKSSGIENGAFQGMKKLSYIRIADTNITSIPQGLPPSLTELHLDGNKISRVDAASLKGLNNLAKLGLSFNSISAVDNGSLANTPHLRELHLDNNKLTRVPGGLAEHKYIQVVYLHNNNISVVGSSDFCPPGHNTKKASYSGVSLFSNPVQYWEIQPSTFRCVYVRSAIQLGNYK FF511E871A1A52DD gpc1_human Glypican-1 GPC1 protein protein human Homo sapiens Human MELRARGWWLLCAAAALVACARGDPASKSRSCGEVRQIYGAKGFSLSDVPQAEISGEHLRICPQGYTCCTSEMEENLANRSHAELETALRDSSRVLQAMLATQLRSFDDHFQHLLNDSERTLQATFPGAFGELYTQNARAFRDLYSELRLYYRGANLHLEETLAEFWARLLERLFKQLHPQLLLPDDYLDCLGKQAEALRPFGEAPRELRLRATRAFVAARSFVQGLGVASDVVRKVAQVPLGPECSRAVMKLVYCAHCLGVPGARPCPDYCRNVLKGCLANQADLDAEWRNLLDSMVLITDKFWGTSGVESVIGSVHTWLAEAINALQDNRDTLTAKVIQGCGNPKVNPQGPGPEEKRRRGKLAPRERPPSGTLEKLVSEAKAQLRDVQDFWISLPGTLCSEKMALSTASDDRCWNGMARGRYLPEVMGDGLANQINNPEVEVDITKPDMTIRQQIMQLKIMTNRLRSAYNGNDVDFQDASDDGSGSGSGDGCLDDLCSRKVSRKSSSSRTPLTHALPGLSEQEGQKTSAASCPQPPTFLLPLLLFLALTVARPRWR Cell surface proteoglycan that bears heparan sulfate mint 16553B56080A83C8 dcn-fam20b-3 1 12 enzyme target enzyme target substrate substrate neutral component neutral component c_terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range P07585:p.Ser34Ala mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate certain certain sequence position certain certain certain sequence position certain S A Wed May 02 14:53:44 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser34ala' Tue Jun 30 19:23:29 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser34Ala' human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. 10 enzyme enzyme neutral component neutral component O75063:p.Asp309Ala mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate certain certain sequence position certain certain certain sequence position certain D A Wed May 02 18:53:28 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp309ala' Tue Jun 30 16:10:12 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp309Ala' trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. phosphorylation phosphorylation reaction phosphorylation 1C, supp 1 fam20b-sdc1-1 1 11 enzyme target enzyme target substrate substrate neutral component neutral component human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. 10 enzyme enzyme neutral component neutral component O75063:p.Asp309Ala mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate certain certain sequence position certain certain certain sequence position certain D A Wed May 02 19:44:20 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp309ala' Tue Jun 30 18:28:02 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp309Ala' trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. phosphorylation phosphorylation reaction phosphorylation supp 2B fam20b-sdc4-1 1 9 enzyme target enzyme target substrate substrate neutral component neutral component human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. 10 enzyme enzyme neutral component neutral component O75063:p.Asp309Ala mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate certain certain sequence position certain certain certain sequence position certain D A Wed May 02 19:00:00 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp309ala' Tue Jun 30 19:13:59 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp309Ala' trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. phosphorylation phosphorylation reaction phosphorylation supp 2A fam20b-sdc4-2 1 8 enzyme target enzyme target substrate substrate neutral component neutral component bovin-cartilage Bos taurus cartilage Cartilage 10 enzyme enzyme neutral component neutral component O75063:p.Asp309Ala mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate certain certain sequence position certain certain certain sequence position certain D A Wed May 02 14:38:12 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp309ala' Tue Jun 30 17:53:09 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp309Ala' trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. phosphorylation phosphorylation reaction phosphorylation supp 2C fam20b-tetra_ben-1 4 5 enzyme target enzyme target substrate substrate neutral component neutral component chemical synthesis Chemical synthesis (Chemical synthesis) 10 enzyme enzyme neutral component neutral component O75063:p.Asp309Ala mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate certain certain sequence position certain certain certain sequence position certain D A Wed May 02 17:28:35 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp309ala' Tue Jun 30 19:25:53 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp309Ala' trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. phosphorylation phosphorylation reaction phosphorylation 1 F/G, supp 3B fam20b-gal_xyl_ben-1 4 6 enzyme target enzyme target substrate substrate neutral component neutral component chemical synthesis Chemical synthesis (Chemical synthesis) 10 enzyme enzyme neutral component neutral component trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. phosphorylation phosphorylation reaction phosphorylation 1G dcn-fam20b-1 1 12 enzyme target enzyme target substrate substrate neutral component neutral component c_terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range human-u2-os Human U2-OS osteosarcoma cell line u2-os U2-OS osteocaroma cell line Not in CAPRI and no primary literature reference 10 enzyme enzyme neutral component neutral component trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. phosphorylation phosphorylation reaction phosphorylation 3A I have included these results as they show the whole gel(?) and it looks "clean". When using FAM20B KO cells most of DCN and GPC1 migrated as a sharp band, implying that FAM20B deletion impaired GAG elongation. dcn-fam20b-2 1 13 enzyme target enzyme target substrate substrate neutral component neutral component c_terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range human-u2-os Human U2-OS osteosarcoma cell line u2-os U2-OS osteocaroma cell line Not in CAPRI and no primary literature reference 10 enzyme enzyme neutral component neutral component trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. phosphorylation phosphorylation reaction phosphorylation 3Ai I have included these results as they show the whole gel(?) and it looks "clean". When using FAM20B KO cells most of DCN and GPC1 migrated as a sharp band, implying that FAM20B deletion impaired GAG elongation. b3galt6-gal_xyl_ben-1 2 6 enzyme target enzyme target substrate substrate neutral component neutral component 2-O-phospho-xylose tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined chemical synthesis Chemical synthesis (Chemical synthesis) 7 enzyme enzyme neutral component neutral component n_terminus mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. enzymatic reaction enzymatic reaction Biochemical reaction Biochemical reaction 4B, Supp 10A In the absence of xylose phosphorylation, GalT-II activity toward Gal-Xyl-Ben was decreased to less than 1% of its activity toward phosphorylated Gal-Xyl(P)-Ben. I could add another molecule to Chebi - but thought this orginal molecule + tag + comment was better? I will go back and re-add a new Chebi compound if you think is appropriate. 234r3qr-1 3 10 enzyme enzyme neutral component neutral component trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. 5 enzyme target enzyme target substrate substrate neutral component neutral component 2-O-phospho-xylose tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined resulting-ptm resulting-ptm resulting ptm resulting-ptm chemical synthesis Chemical synthesis (Chemical synthesis) phosphorylation phosphorylation reaction phosphorylation Supp 3 C/D fam20b-tetra_ben-2 3 10 enzyme enzyme neutral component neutral component trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. 12 enzyme target enzyme target substrate substrate neutral component neutral component human-u2-os Human U2-OS osteosarcoma cell line u2-os U2-OS osteocaroma cell line Not in CAPRI and no primary literature reference phosphorylation phosphorylation reaction phosphorylation Supp 7 C