UniProt UniProt http://www.uniprot.org Rho of plant GTPase signaling regulates the behavior of Arabidopsis kinesin-13A to establish secondary cell wall patterns. Rho of plant GTPase signaling regulates the behavior of Arabidopsis kinesin-13A to establish secondary cell wall patterns. The Plant cell 2013 imex curation Oda Y., Fukuda H. oda@biol.s.u-tokyo.ac.jp 15-Sep-2016: Contacted by IntAct-Help. Only protein-protein interactions tobac-leaf Nicotiana tabacum (tobacco) leaf leaf leaf bifc bimolecular fluorescence complementation bifc tag fluorescence tag visualisation by fluorescence tag fluorescence Oda Y., Fukuda H. oda@biol.s.u-tokyo.ac.jp Accepted 2016-AUG-09 AT 15:21 BST AT 15:21 BST by MGT The Plant cell 2013 imex curation Fig.3C and Fig.3D (in Arabidopsis culture) were not added as they represented localization/association with microtubules (no precised tubulins). Only Fig.3B was retained as it was the only obvious interaction.; Modifications done. Thank you for suggesting the Exp.Mod. comment. I was not aware about this kind of annotations. Rho of plant GTPase signaling regulates the behavior of Arabidopsis kinesin-13A to establish secondary cell wall patterns. Rho of plant GTPase signaling regulates the behavior of Arabidopsis kinesin-13A to establish secondary cell wall patterns. The Plant cell 2013 imex curation Oda Y., Fukuda H. oda@biol.s.u-tokyo.ac.jp 15-Sep-2016: Contacted by IntAct-Help. Only protein-protein interactions arath-cell-culture Arabidopsis thaliana cell culture plant_cell_culture plant cellular culture The culture conditions should always be cited in the experimental modifications annotation Either whole plant or undifferentiated plant cells maintained in culture. confocal microscopy confocal microscopy tag fluorescence tag visualisation by fluorescence tag fluorescence Oda Y., Fukuda H. Arabidopsis differentiating xylem cells (Fig. 3A). Arabidopsis non-xylem cultured cells (Fig.8). oda@biol.s.u-tokyo.ac.jp Accepted 2016-AUG-09 AT 15:21 BST AT 15:21 BST by MGT The Plant cell 2013 imex curation Fig.3C and Fig.3D (in Arabidopsis culture) were not added as they represented localization/association with microtubules (no precised tubulins). Only Fig.3B was retained as it was the only obvious interaction.; Modifications done. Thank you for suggesting the Exp.Mod. comment. I was not aware about this kind of annotations. Rho of plant GTPase signaling regulates the behavior of Arabidopsis kinesin-13A to establish secondary cell wall patterns. Rho of plant GTPase signaling regulates the behavior of Arabidopsis kinesin-13A to establish secondary cell wall patterns. The Plant cell 2013 imex curation Oda Y., Fukuda H. oda@biol.s.u-tokyo.ac.jp 15-Sep-2016: Contacted by IntAct-Help. Only protein-protein interactions arath-cell-culture Arabidopsis thaliana cell culture plant_cell_culture plant cellular culture The culture conditions should always be cited in the experimental modifications annotation Either whole plant or undifferentiated plant cells maintained in culture. bifc bimolecular fluorescence complementation bifc tag fluorescence tag visualisation by fluorescence tag fluorescence Oda Y., Fukuda H. Arabidopsis differentiating xylem cells oda@biol.s.u-tokyo.ac.jp Accepted 2016-AUG-09 AT 15:21 BST AT 15:21 BST by MGT The Plant cell 2013 imex curation Fig.3C and Fig.3D (in Arabidopsis culture) were not added as they represented localization/association with microtubules (no precised tubulins). Only Fig.3B was retained as it was the only obvious interaction.; Modifications done. Thank you for suggesting the Exp.Mod. comment. I was not aware about this kind of annotations. icr5_arath Interactor of constitutive active ROPs 5 ICR5 MIDD1 Protein MICROTUBULE DEPLETION DOMAIN 1 RIP3 ROP-interactive partner 3 F4P12.50 At3g53350 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MQTPKSRPGSLELPQKKSPLPAPKVVRRLKPSGAESDPKTKTISKTQIPKVVADRRSARIPLNEIQKKRTGRIPELESTISQLQEELKKAKEELNRSEALKREAQEEAEDAKHQLMDINASEDSRIEELRKLSQERDKTWQSELEAMQRQHGMDSTALSSAINEVQKLKSKLFESESELEQSKYEVRSLEKLVRQLEEERVNSRDSSSSMEVEELKEAMNLSRQEITQLKSAVEAAETRYQEEYIQSTLQIRSAYEQTEAVKSRYSQREAELTEELNRTKDEIEGLRKELMEKVKEDESTGDLKKLESDLMEVRGSLMDKEMELQILRSAMEKKVETANTEAMEAELKRVKIQCEQWRKAAETAASILNNDEERTDSIETSKMLKKFGVLLKKNHK 4A0CE7D68B2C8E70 kn13a_arath Kinesin-like protein KIN-13A KIN13A AtKINESIN-13A KINESIN-13A MGL6.9 At3g16630 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MGGQMQQNNAAAATALYDGALPTNDAGDAVMARWLQSAGLQHLASPVASTGNDQRHLPNLLMQGYGAQTAEEKQRLFQLMRNLNFNGESTSESYTPTAHTSAAMPSSEGFFSPEFRGDFGAGLLDLHAMDDTELLSEHVITEPFEPSPFMPSVNKEFEEDYNLAANRQQRQQTEAEPLGLLPKSDKENNSVAKIKVVVRKRPLNKKETAKKEEDVVTVSDNSLTVHEPRVKVDLTAYVEKHEFCFDAVLDEDVSNDEVYRATIEPIIPIIFQRTKATCFAYGQTGSGKTFTMKPLPIRAVEDLMRLLRQPVYSNQRFKLWLSYFEIYGGKLFDLLSERKKLCMREDGRQQVCIVGLQEYEVSDVQIVKDFIEKGNAERSTGSTGANEESSRSHAILQLVVKKHVEVKDTRRRNNDSNELPGKVVGKISFIDLAGSERGADTTDNDRQTRIEGAEINKSLLALKECIRALDNDQLHIPFRGSKLTEVLRDSFVGNSRTVMISCISPNAGSCEHTLNTLRYADRVKSLSKSGNSKKDQTANSMPPVNKDPLLGPNDVEDVFEPPQEVNVPETRRRVVEKDSNSSTSGIDFRQPTNYREESGIPSFSMDKGRSEPNSSFAGSTSQRNNISSYPQETSDREEKVKKVSPPRGKGLREEKPDRPQNWSKRDVSSSDIPTLTNFRQNASETASRQYETASRQYETDPSLDENLDALLEEEEALIAAHRKEIEDTMEIVREEMKLLAEVDQPGSMIENYVTQLSFVLSRKAAGLVSLQARLARFQHRLKEQEILSRKRVPR 13CBD4DDBEE2454B icr5-kin13a-1 1 4 unspecified role unspecified role neutral component neutral component region cyfp cYFP undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 5 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region nyfp nYFP undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Fig. 3B kin13a-irc5-2 2 4 unspecified role unspecified role neutral component neutral component region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 5 unspecified role unspecified role neutral component neutral component region rfp tag red fluorescent protein tag red fluorescent protein RFP red fluorescent protein RFP rfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain colocalization colocalization Fig. 3A, Fig. 8 MIDD1 and Kinesin-13A delta_M colocalized in the membrane domains underlying secondary cell wall pits. icr5-kinesin_13a-1 3 4 unspecified role unspecified role neutral component neutral component region cyfp cYFP undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 5 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region nyfp nYFP undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Fig. 3C The YFP fluorescent signal was detected in the secondary cell wall pits.