UniProt UniProt http://www.uniprot.org Pathogenic NPHP5 mutations impair protein interaction with Cep290, a prerequisite for ciliogenesis. Pathogenic NPHP5 mutations impair protein interaction with Cep290, a prerequisite for ciliogenesis. Human molecular genetics 2013 imex curation Barbelanne M., Song J., Ahmadzai M., Tsang WY. Rare diseases - Interactions investigated in the context of Rare genetic disease william.tsang@ircm.qc.ca 22-Jun-2016: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Barbelanne M., Song J., Ahmadzai M., Tsang WY. Rare diseases - Interactions investigated in the context of Rare genetic disease william.tsang@ircm.qc.ca Accepted 2016-JUN-10 AT 11:40 BST AT 11:40 BST by MGT Human molecular genetics 2013 Only protein-protein interactions imex curation imex curation Pathogenic NPHP5 mutations impair protein interaction with Cep290, a prerequisite for ciliogenesis. Pathogenic NPHP5 mutations impair protein interaction with Cep290, a prerequisite for ciliogenesis. Human molecular genetics 2013 imex curation Barbelanne M., Song J., Ahmadzai M., Tsang WY. Rare diseases - Interactions investigated in the context of Rare genetic disease william.tsang@ircm.qc.ca 22-Jun-2016: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-htert_rpe1 Homo sapiens htert_rpe1 htert_rpe1 fluorescence imaging fluorescence microscopy fluorescence imaging immunocytochemistry immunocytochemistry Barbelanne M., Song J., Ahmadzai M., Tsang WY. Rare diseases - Interactions investigated in the context of Rare genetic disease william.tsang@ircm.qc.ca Accepted 2016-JUN-10 AT 11:40 BST AT 11:40 BST by MGT Human molecular genetics 2013 Only protein-protein interactions imex curation imex curation Pathogenic NPHP5 mutations impair protein interaction with Cep290, a prerequisite for ciliogenesis. Pathogenic NPHP5 mutations impair protein interaction with Cep290, a prerequisite for ciliogenesis. Human molecular genetics 2013 imex curation Barbelanne M., Song J., Ahmadzai M., Tsang WY. Rare diseases - Interactions investigated in the context of Rare genetic disease william.tsang@ircm.qc.ca 22-Jun-2016: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Barbelanne M., Song J., Ahmadzai M., Tsang WY. Rare diseases - Interactions investigated in the context of Rare genetic disease william.tsang@ircm.qc.ca Accepted 2016-JUN-10 AT 11:40 BST AT 11:40 BST by MGT Human molecular genetics 2013 Only protein-protein interactions imex curation imex curation Pathogenic NPHP5 mutations impair protein interaction with Cep290, a prerequisite for ciliogenesis. Pathogenic NPHP5 mutations impair protein interaction with Cep290, a prerequisite for ciliogenesis. Human molecular genetics 2013 imex curation Barbelanne M., Song J., Ahmadzai M., Tsang WY. Rare diseases - Interactions investigated in the context of Rare genetic disease william.tsang@ircm.qc.ca 22-Jun-2016: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro far western blotting far western blotting Affinity blotting Affinity blotting anti tag western anti tag western blot anti tag western Barbelanne M., Song J., Ahmadzai M., Tsang WY. Rare diseases - Interactions investigated in the context of Rare genetic disease william.tsang@ircm.qc.ca Accepted 2016-JUN-10 AT 11:40 BST AT 11:40 BST by MGT Human molecular genetics 2013 Only protein-protein interactions imex curation imex curation Pathogenic NPHP5 mutations impair protein interaction with Cep290, a prerequisite for ciliogenesis. Pathogenic NPHP5 mutations impair protein interaction with Cep290, a prerequisite for ciliogenesis. Human molecular genetics 2013 imex curation Barbelanne M., Song J., Ahmadzai M., Tsang WY. Rare diseases - Interactions investigated in the context of Rare genetic disease william.tsang@ircm.qc.ca 22-Jun-2016: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Barbelanne M., Song J., Ahmadzai M., Tsang WY. Rare diseases - Interactions investigated in the context of Rare genetic disease william.tsang@ircm.qc.ca Accepted 2016-JUN-10 AT 11:40 BST AT 11:40 BST by MGT Human molecular genetics 2013 Only protein-protein interactions imex curation imex curation Pathogenic NPHP5 mutations impair protein interaction with Cep290, a prerequisite for ciliogenesis. Pathogenic NPHP5 mutations impair protein interaction with Cep290, a prerequisite for ciliogenesis. Human molecular genetics 2013 imex curation Barbelanne M., Song J., Ahmadzai M., Tsang WY. Rare diseases - Interactions investigated in the context of Rare genetic disease william.tsang@ircm.qc.ca 22-Jun-2016: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Barbelanne M., Song J., Ahmadzai M., Tsang WY. Rare diseases - Interactions investigated in the context of Rare genetic disease william.tsang@ircm.qc.ca Accepted 2016-JUN-10 AT 11:40 BST AT 11:40 BST by MGT Human molecular genetics 2013 Only protein-protein interactions imex curation imex curation Pathogenic NPHP5 mutations impair protein interaction with Cep290, a prerequisite for ciliogenesis. Pathogenic NPHP5 mutations impair protein interaction with Cep290, a prerequisite for ciliogenesis. Human molecular genetics 2013 imex curation Barbelanne M., Song J., Ahmadzai M., Tsang WY. Rare diseases - Interactions investigated in the context of Rare genetic disease william.tsang@ircm.qc.ca 22-Jun-2016: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Barbelanne M., Song J., Ahmadzai M., Tsang WY. Rare diseases - Interactions investigated in the context of Rare genetic disease Interaction using Flag-CaM1234 as bait have been omitted as details of the mutations cannot be traced. william.tsang@ircm.qc.ca Accepted 2016-JUN-10 AT 11:40 BST AT 11:40 BST by MGT Human molecular genetics 2013 Only protein-protein interactions imex curation imex curation calm_human Calmodulin CALM1 CALM CAM CAM1 CALM2 CAMB CALM3 CAM3 CAMC CAMIII CAM2 CALML2 protein protein human Homo sapiens Human MADQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGYISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK DIP protein P62158 original sequence version: 97 DIP protein P62158 original sequence version: 144 DIP protein P62158 original sequence version: 146 DIP protein P62158 original sequence version: 73 DIP protein P62158 original sequence version: 102 DIP protein P62158 original sequence version: 98 DIP protein P62158 original sequence version: 96 DIP protein P62158 original sequence version: 111 DIP protein P62158 original sequence version: 131 DIP protein P62158 original sequence version: 136 DIP protein P62158 original sequence version: 145 DIP protein P62158 original sequence version: 154 DIP protein P62158 original sequence version: 148 DIP protein P62158 original sequence version: 116 DIP protein P62158 original sequence version: 120 DIP protein P62158 original sequence version: 130 6B4BC3FCDE10727B ce290_human Centrosomal protein of 290 kDa CEP290 KIAA0373 NPHP6 Nephrocystin-6 Tumor antigen se2-2 Cancer/testis antigen 87 BBS14 Bardet-Biedl syndrome 14 protein protein protein human Homo sapiens Human MPPNINWKEIMKVDPDDLPRQEELADNLLISLSKVEVNELKSEKQENVIHLFRITQSLMKMKAQEVELALEEVEKAGEEQAKFENQLKTKVMKLENELEMAQQSAGGRDTRFLRNEICQLEKQLEQKDRELEDMEKELEKEKKVNEQLALRNEEAENENSKLRRENKRLKKKNEQLCQDIIDYQKQIDSQKETLLSRRGEDSDYRSQLSKKNYELIQYLDEIQTLTEANEKIEVQNQEMRKNLEESVQEMEKMTDEYNRMKAIVHQTDNVIDQLKKENDHYQLQVQELTDLLKSKNEEDDPIMVAVNAKVEEWKLILSSKDDEIIEYQQMLHNLREKLKNAQLDADKSNVMALQQGIQERDSQIKMLTEQVEQYTKEMEKNTCIIEDLKNELQRNKGASTLSQQTHMKIQSTLDILKEKTKEAERTAELAEADAREKDKELVEALKRLKDYESGVYGLEDAVVEIKNCKNQIKIRDREIEILTKEINKLELKISDFLDENEALRERVGLEPKTMIDLTEFRNSKHLKQQQYRAENQILLKEIESLEEERLDLKKKIRQMAQERGKRSATSGLTTEDLNLTENISQGDRISERKLDLLSLKNMSEAQSKNEFLSRELIEKERDLERSRTVIAKFQNKLKELVEENKQLEEGMKEILQAIKEMQKDPDVKGGETSLIIPSLERLVNAIESKNAEGIFDASLHLKAQVDQLTGRNEELRQELRESRKEAINYSQQLAKANLKIDHLEKETSLLRQSEGSNVVFKGIDLPDGIAPSSASIINSQNEYLIHLLQELENKEKKLKNLEDSLEDYNRKFAVIRHQQSLLYKEYLSEKETWKTESKTIKEEKRKLEDQVQQDAIKVKEYNNLLNALQMDSDEMKKILAENSRKITVLQVNEKSLIRQYTTLVELERQLRKENEKQKNELLSMEAEVCEKIGCLQRFKEMAIFKIAALQKVVDNSVSLSELELANKQYNELTAKYRDILQKDNMLVQRTSNLEHLECENISLKEQVESINKELEITKEKLHTIEQAWEQETKLGNESSMDKAKKSITNSDIVSISKKITMLEMKELNERQRAEHCQKMYEHLRTSLKQMEERNFELETKFAELTKINLDAQKVEQMLRDELADSVSKAVSDADRQRILELEKNEMELKVEVSKLREISDIARRQVEILNAQQQSRDKEVESLRMQLLDYQAQSDEKSLIAKLHQHNVSLQLSEATALGKLESITSKLQKMEAYNLRLEQKLDEKEQALYYARLEGRNRAKHLRQTIQSLRRQFSGALPLAQQEKFSKTMIQLQNDKLKIMQEMKNSQQEHRNMENKTLEMELKLKGLEELISTLKDTKGAQKVINWHMKIEELRLQELKLNRELVKDKEEIKYLNNIISEYERTISSLEEEIVQQNKFHEERQMAWDQREVDLERQLDIFDRQQNEILNAAQKFEEATGSIPDPSLPLPNQLEIALRKIKENIRIILETRATCKSLEEKLKEKESALRLAEQNILSRDKVINELRLRLPATAEREKLIAELGRKEMEPKSHHTLKIAHQTIANMQARLNQKEEVLKKYQRLLEKAREEQREIVKKHEEDLHILHHRLELQADSSLNKFKQTAWDLMKQSPTPVPTNKHFIRLAEMEQTVAEQDDSLSSLLVKLKKVSQDLERQREITELKVKEFENIKLQLQENHEDEVKKVKAEVEDLKYLLDQSQKESQCLKSELQAQKEANSRAPTTTMRNLVERLKSQLALKEKQQKALSRALLELRAEMTAAAEERIISATSQKEAHLNVQQIVDRHTRELKTQVEDLNENLLKLKEALKTSKNRENSLTDNLNDLNNELQKKQKAYNKILREKEEIDQENDELKRQIKRLTSGLQGKPLTDNKQSLIEELQRKVKKLENQLEGKVEEVDLKPMKEKNAKEELIRWEEGKKWQAKIEGIRNKLKEKEGEVFTLTKQLNTLKDLFAKADKEKLTLQRKLKTTGMTVDQVLGIRALESEKELEELKKRNLDLENDILYMRAHQALPRDSVVEDLHLQNRYLQEKLHALEKQFSKDTYSKPSISGIESDDHCQREQELQKENLKLSSENIELKFQLEQANKDLPRLKNQVRDLKEMCEFLKKEKAEVQRKLGHVRGSGRSGKTIPELEKTIGLMKKVVEKVQRENEQLKKASGILTSEKMANIEQENEKLKAELEKLKAHLGHQLSMHYESKTKGTEKIIAENERLRKELKKETDAAEKLRIAKNNLEILNEKMTVQLEETGKRLQFAESRGPQLEGADSKSWKSIVVTRMYETKLKELETDIAKKNQSITDLKQLVKEATEREQKVNKYNEDLEQQIKILKHVPEGAETEQGLKRELQVLRLANHQLDKEKAELIHQIEANKDQSGAESTIPDADQLKEKIKDLETQLKMSDLEKQHLKEEIKKLKKELENFDPSFFEEIEDLKYNYKEEVKKNILLEEKVKKLSEQLGVELTSPVAASEEFEDEEESPVNFPIY DIP protein O15078 original sequence version: 125 7CA87D53FAF036FC iqcb1_human IQ calmodulin-binding motif-containing protein 1 KIAA0036 NPHP5 Nephrocystin-5 IQCB1 p53 and DNA damage-regulated IQ motif protein OK/SW-cl.85 protein protein human Homo sapiens Human MKPTGTDPRILSIAAEVAKSPEQNVPVILLKLKEIINITPLGSSELKKIKQDIYCYDLIQYCLLVLSQDYSRIQGGWTTISQLTQILSHCCVGLEPGEDAEEFYNELLPSAAENFLVLGRQLQTCFINAAKAEEKDELLHFFQIVTDSLFWLLGGHVELIQNVLQSDHFLHLLQADNVQIGSAVMMMLQNILQINSGDLLRIGRKALYSILDEVIFKLFSTPSPVIRSTATKLLLLMAESHQEILILLRQSTCYKGLRRLLSKQETGTEFSQELRQLVGLLSPMVYQEVEEQKLHQAACLIQAYWKGFQTRKRLKKLPSAVIALQRSFRSKRSKMLLEINRQKEEEDLKLQLQLQRQRAMRLSRELQLSMLEIVHPGQVEKHYREMEEKSALIIQKHWRGYRERKNFHQQRQSLIEYKAAVTLQRAALKFLAKCRKKKKLFAPWRGLQELTDARRVELKKRVDDYVRRHLGSPMSDVVSRELHAQAQERLQHYFMGRALEERAQQHREALIAQISTNVEQLMKAPSLKEAEGKEPELFLSRSRPVAAKAKQAHLTTLKHIQAPWWKKLGEESGDEIDVPKDELSIELENLFIGGTKPP 5589FDE6B0D15D78 calm_rat Calmodulin Calm1 Calm Cam Cam1 Calm2 Cam2 Camb Calm3 Cam3 Camc CaMII protein protein rat Rattus norvegicus (Rat) Rat MADQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGYISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK DIP protein P62161 original sequence version: 125 DIP protein P62161 original sequence version: 51 DIP protein P62161 original sequence version: 55 DIP protein P62161 original sequence version: 59 DIP protein P62161 original sequence version: 80 DIP protein P62161 original sequence version: 81 DIP protein P62161 original sequence version: 132 DIP protein P62161 original sequence version: 96 6B4BC3FCDE10727B iqcb1-calm1-1 3 9 unspecified role unspecified role prey prey 8 unspecified role unspecified role prey prey 10 unspecified role unspecified role bait bait association association 3A cep290-calm1-1 3 9 unspecified role unspecified role bait bait 8 unspecified role unspecified role prey prey 10 unspecified role unspecified role prey prey association association 3A iqcb1-calm1-2 5 9 unspecified role unspecified role prey prey 8 unspecified role unspecified role prey prey 10 unspecified role unspecified role bait bait nucl transfection nucleic acid transfection nucl transfection region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Q15051:p.Ala547Lys mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain A K Wed May 02 17:10:06 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ala547lys' Tue Jun 30 16:10:12 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ala547Lys' Q15051:p.Lys550Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain K A Wed May 02 15:11:20 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys550ala' Tue Jun 30 17:43:08 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys550Ala' Q15051:p.Gln551Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain Q A Wed May 02 16:28:56 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gln551ala' Tue Jun 30 17:07:47 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln551Ala' Q15051:p.Lys548Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain K A Wed May 02 19:21:31 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys548ala' Tue Jun 30 17:38:42 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys548Ala' association association 3B iqcb1-calm1-3 5 8 unspecified role unspecified role prey prey 10 unspecified role unspecified role bait bait nucl transfection nucleic acid transfection nucl transfection Q15051:p.Ala547Lys mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain A K Wed May 02 14:48:52 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ala547lys' Tue Jun 30 19:52:40 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ala547Lys' Q15051:p.Lys548Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain K A Wed May 02 16:19:32 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys548ala' Tue Jun 30 18:42:45 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys548Ala' Q15051:p.Ala549Lys mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain A K Wed May 02 15:02:14 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ala549lys' Tue Jun 30 19:51:14 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ala549Lys' region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain no interaction with CEP290 region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Q15051:p.Ile393Asn mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain I N mutation retains interaction with CEP290 Wed May 02 16:20:27 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ile393asn' Tue Jun 30 19:33:08 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ile393Asn' IQ regions 1-3 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain Single IQ motif deletion does not change interaction with calmodulin and CEP290. Triple deletion IQ123 disrupts the interaction with calmodulin but retains interaction with CEP290. Q15051:p.Lys550Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain K A Wed May 02 17:34:56 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys550ala' Tue Jun 30 15:50:33 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys550Ala' Q15051:p.Gln551Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain Q A Wed May 02 16:06:35 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gln551ala' Tue Jun 30 19:40:22 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln551Ala' physical association physical association 3B, 5A,6 Complete loss of binding was achieved only when all three IQ motifs were deleted. Two disease associated mutations of NPHP5 - F142fsX146 (424–425delTT,) and R332X result in proteins that did not interact with CAM. cep290-iqcb1-2 7 9 unspecified role unspecified role bait bait nucl transfection nucleic acid transfection nucl transfection region required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 10 unspecified role unspecified role prey prey nucl transfection nucleic acid transfection nucl transfection region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association supplemental fig. S3A cep290-calm1-2 7 9 unspecified role unspecified role bait bait nucl transfection nucleic acid transfection nucl transfection region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 8 western blot western blot Immuno blot Immuno blot anti tag western anti tag western blot anti tag western unspecified role unspecified role prey prey cell lysate cell lysate 10 unspecified role unspecified role prey prey nucl transfection nucleic acid transfection nucl transfection region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association supplemental fig. S3A cep290-calm1-3 5 9 unspecified role unspecified role bait bait nucl transfection nucleic acid transfection nucl transfection region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain also binds IQCB1/NPHP5 8 western blot western blot Immuno blot Immuno blot western blot western blot Immuno blot Immuno blot unspecified role unspecified role prey prey cell lysate cell lysate association association supplemental fig. S3A cep290-iqcb1-3 4 9 unspecified role unspecified role prey prey region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. 10 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) direct interaction direct interaction supplemental fig. S3c calm1-cep290-1 7 9 western blot western blot Immuno blot Immuno blot anti tag western anti tag western blot anti tag western unspecified role unspecified role prey prey cell lysate cell lysate 11 unspecified role unspecified role bait bait nucl transfection nucleic acid transfection nucl transfection region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 10 unspecified role unspecified role prey prey nucl transfection nucleic acid transfection nucl transfection region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association supplemental fig. S5B calm1-cep290-2 1 9 western blot western blot Immuno blot Immuno blot western blot western blot Immuno blot Immuno blot unspecified role unspecified role prey prey 8 unspecified role unspecified role prey prey 10 unspecified role unspecified role bait bait nucl transfection nucleic acid transfection nucl transfection region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association supplemental fig S5A EGTA iqcb1-calm1-4 6 8 unspecified role unspecified role prey prey purified purified ecoli Escherichia coli (strain K12) 10 unspecified role unspecified role bait bait purified purified region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) direct interaction direct interaction supplemental fig S5C iqcb1-calm1-5 5 9 unspecified role unspecified role prey prey 10 unspecified role unspecified role bait bait nucl transfection nucleic acid transfection nucl transfection region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain deletion disrupts interaction with CEP290 but retains interaction with calmodulin region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain deletion disrupts interaction with CEP290 but retains interaction with calmodulin Q15051:p.Ala549Lys mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain A K mutation disrupts interaction with CEP290 but retains interaction with calmodulin Wed May 02 18:45:34 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ala549lys' Tue Jun 30 19:07:11 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ala549Lys' physical association physical association 3Bi, 6 Several disease associated NPHP5 mutations -(F142fsX146, R332X, R461X, R502X and H506fsX518) result in proteins that do not bind to CEP290 calm1-cep290-3 7 9 western blot western blot Immuno blot Immuno blot anti tag western anti tag western blot anti tag western unspecified role unspecified role prey prey cell lysate cell lysate 11 unspecified role unspecified role bait bait nucl transfection nucleic acid transfection nucl transfection region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association supplemental fig. S5Bi 25345-1 2 9 unspecified role unspecified role neutral component neutral component 10 unspecified role unspecified role neutral component neutral component colocalization colocalization 1D cep290-iqcb1-5 2 9 unspecified role unspecified role neutral component neutral component 10 unspecified role unspecified role neutral component neutral component region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined colocalization colocalization Supp 5D