IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Mammalian α arrestins link activated seven transmembrane receptors to Nedd4 family e3 ubiquitin ligases and interact with β arrestins. Mammalian α arrestins link activated seven transmembrane receptors to Nedd4 family e3 ubiquitin ligases and interact with β arrestins. PloS one 2011 imex curation Shea FF., Rowell JL., Li Y., Chang TH., Alvarez CE. Dark Space Dataset = no14? alvarez.73@osu.edu 11-Apr-2016: Contacted by IntAct-Help. Only protein-protein interactions imex curation yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Shea FF., Rowell JL., Li Y., Chang TH., Alvarez CE. Dark Space Dataset = no14? ARRDC1 tail region found from Ref 15, Fig2. ARRDC3 tail region is given in Supp fig 1. alvarez.73@osu.edu Accepted 2016-APR-05 AT 08:22 BST AT 08:22 BST by ORCHARD PloS one 2011 Only protein-protein interactions imex curation imex curation EBI-11674311/EBI-11675497:-PY bands- I dont think this is meant to be a "mutation increasing" - the greater band is due to "Notably, the protein levels of aArr-PYmut are always very significantly higher than WT." - as shown by WB in Supp Fig 2C (no IP).- so due to increased expression of the mutant that they cannot explain.; done; I have linked as you ask - but the manual states:- "Domains must be shown to interact in the same interaction, and should be precisely defined – a fragment of unknown coordinates should not be linked". Also I have added the "WW" domain XRef - although the constructs used contain several WW domains; I had been following the "rule" (which I can no longer find in the manual) that an Interpro domain sequence length should correspond to ~90% to the construct sequence - other wise does this meant we have to add an InterPro XRef when ever any sequence includes ANY domain ( which they usually do)?? I had added the domain(s) info in the short label only. I have added the positive controls as you ask - but as these are commercially available products and not mentioned in the methods section I had assumed they may not be human. The Invitrogen web site gives no species for either construct - and no sequence I can blat either. The mutations given for RalGDS (m1= I (L?)77T and L65P do not correspond to any human sequence (- nor mouse/rat.) Mammalian α arrestins link activated seven transmembrane receptors to Nedd4 family e3 ubiquitin ligases and interact with β arrestins. Mammalian α arrestins link activated seven transmembrane receptors to Nedd4 family e3 ubiquitin ligases and interact with β arrestins. PloS one 2011 imex curation Shea FF., Rowell JL., Li Y., Chang TH., Alvarez CE. Dark Space Dataset = no14? alvarez.73@osu.edu 11-Apr-2016: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Shea FF., Rowell JL., Li Y., Chang TH., Alvarez CE. Dark Space Dataset = no14? alvarez.73@osu.edu Accepted 2016-APR-05 AT 08:22 BST AT 08:22 BST by ORCHARD PloS one 2011 Only protein-protein interactions imex curation imex curation EBI-11674311/EBI-11675497:-PY bands- I dont think this is meant to be a "mutation increasing" - the greater band is due to "Notably, the protein levels of aArr-PYmut are always very significantly higher than WT." - as shown by WB in Supp Fig 2C (no IP).- so due to increased expression of the mutant that they cannot explain.; done; I have linked as you ask - but the manual states:- "Domains must be shown to interact in the same interaction, and should be precisely defined – a fragment of unknown coordinates should not be linked". Also I have added the "WW" domain XRef - although the constructs used contain several WW domains; I had been following the "rule" (which I can no longer find in the manual) that an Interpro domain sequence length should correspond to ~90% to the construct sequence - other wise does this meant we have to add an InterPro XRef when ever any sequence includes ANY domain ( which they usually do)?? I had added the domain(s) info in the short label only. I have added the positive controls as you ask - but as these are commercially available products and not mentioned in the methods section I had assumed they may not be human. The Invitrogen web site gives no species for either construct - and no sequence I can blat either. The mutations given for RalGDS (m1= I (L?)77T and L65P do not correspond to any human sequence (- nor mouse/rat.) Mammalian α arrestins link activated seven transmembrane receptors to Nedd4 family e3 ubiquitin ligases and interact with β arrestins. Mammalian α arrestins link activated seven transmembrane receptors to Nedd4 family e3 ubiquitin ligases and interact with β arrestins. PloS one 2011 imex curation Shea FF., Rowell JL., Li Y., Chang TH., Alvarez CE. Dark Space Dataset = no14? alvarez.73@osu.edu 11-Apr-2016: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. confocal microscopy confocal microscopy immunocytochemistry immunocytochemistry Shea FF., Rowell JL., Li Y., Chang TH., Alvarez CE. Dark Space Dataset = no14? alvarez.73@osu.edu Accepted 2016-APR-05 AT 08:22 BST AT 08:22 BST by ORCHARD PloS one 2011 Only protein-protein interactions imex curation imex curation EBI-11674311/EBI-11675497:-PY bands- I dont think this is meant to be a "mutation increasing" - the greater band is due to "Notably, the protein levels of aArr-PYmut are always very significantly higher than WT." - as shown by WB in Supp Fig 2C (no IP).- so due to increased expression of the mutant that they cannot explain.; done; I have linked as you ask - but the manual states:- "Domains must be shown to interact in the same interaction, and should be precisely defined – a fragment of unknown coordinates should not be linked". Also I have added the "WW" domain XRef - although the constructs used contain several WW domains; I had been following the "rule" (which I can no longer find in the manual) that an Interpro domain sequence length should correspond to ~90% to the construct sequence - other wise does this meant we have to add an InterPro XRef when ever any sequence includes ANY domain ( which they usually do)?? I had added the domain(s) info in the short label only. I have added the positive controls as you ask - but as these are commercially available products and not mentioned in the methods section I had assumed they may not be human. The Invitrogen web site gives no species for either construct - and no sequence I can blat either. The mutations given for RalGDS (m1= I (L?)77T and L65P do not correspond to any human sequence (- nor mouse/rat.) Mammalian α arrestins link activated seven transmembrane receptors to Nedd4 family e3 ubiquitin ligases and interact with β arrestins. Mammalian α arrestins link activated seven transmembrane receptors to Nedd4 family e3 ubiquitin ligases and interact with β arrestins. PloS one 2011 imex curation Shea FF., Rowell JL., Li Y., Chang TH., Alvarez CE. Dark Space Dataset = no14? alvarez.73@osu.edu 11-Apr-2016: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Shea FF., Rowell JL., Li Y., Chang TH., Alvarez CE. Interactions from Figs 6 C/D have not been added due to lack of antibody specificity. both AFFB1 and B2 have more than one isoform listed; although 2 bands are shown, it is not possible to tell if these are from both proteins or two isoforms of one protein. Dark Space Dataset = no14? alvarez.73@osu.edu Accepted 2016-APR-05 AT 08:22 BST AT 08:22 BST by ORCHARD PloS one 2011 Only protein-protein interactions imex curation imex curation EBI-11674311/EBI-11675497:-PY bands- I dont think this is meant to be a "mutation increasing" - the greater band is due to "Notably, the protein levels of aArr-PYmut are always very significantly higher than WT." - as shown by WB in Supp Fig 2C (no IP).- so due to increased expression of the mutant that they cannot explain.; done; I have linked as you ask - but the manual states:- "Domains must be shown to interact in the same interaction, and should be precisely defined – a fragment of unknown coordinates should not be linked". Also I have added the "WW" domain XRef - although the constructs used contain several WW domains; I had been following the "rule" (which I can no longer find in the manual) that an Interpro domain sequence length should correspond to ~90% to the construct sequence - other wise does this meant we have to add an InterPro XRef when ever any sequence includes ANY domain ( which they usually do)?? I had added the domain(s) info in the short label only. I have added the positive controls as you ask - but as these are commercially available products and not mentioned in the methods section I had assumed they may not be human. The Invitrogen web site gives no species for either construct - and no sequence I can blat either. The mutations given for RalGDS (m1= I (L?)77T and L65P do not correspond to any human sequence (- nor mouse/rat.) gnds_human Ral guanine nucleotide dissociation stimulator RALGDS RGF KIAA1308 Ral guanine nucleotide exchange factor protein protein human Homo sapiens Human MVQRMWAEAAGPAGGAEPLFPGSRRSRSVWDAVRLEVGVPDSCPVVLHSFTQLDPDLPRPESSTQEIGEELINGVIYSISLRKVQLHHGGNKGQRWLGYENESALNLYETCKVRTVKAGTLEKLVEHLVPAFQGSDLSYVTIFLCTYRAFTTTQQVLDLLFKRYGRCDALTASSRYGCILPYSDEDGGPQDQLKNAISSILGTWLDQYSEDFCQPPDFPCLKQLVAYVQLNMPGSDLERRAHLLLAQLEHSEPIEAEPEALSPVPALKPTPELELALTPARAPSPVPAPAPEPEPAPTPAPGSELEVAPAPAPELQQAPEPAVGLESAPAPALELEPAPEQDPAPSQTLELEPAPAPVPSLQPSWPSPVVAENGLSEEKPHLLVFPPDLVAEQFTLMDAELFKKVVPYHCLGSIWSQRDKKGKEHLAPTIRATVTQFNSVANCVITTCLGNRSTKAPDRARVVEHWIEVARECRILKNFSSLYAILSALQSNSIHRLKKTWEDVSRDSFRIFQKLSEIFSDENNYSLSRELLIKEGTSKFATLEMNPKRAQKRPKETGIIQGTVPYLGTFLTDLVMLDTAMKDYLYGRLINFEKRRKEFEVIAQIKLLQSACNNYSIAPDEQFGAWFRAVERLSETESYNLSCELEPPSESASNTLRTKKNTAIVKRWSDRQAPSTELSTSGSSHSKSCDQLRCGPYLSSGDIADALSVHSAGSSSSDVEEINISFVPESPDGQEKKFWESASQSSPETSGISSASSSTSSSSASTTPVAATRTHKRSVSGLCNSSSALPLYNQQVGDCCIIRVSLDVDNGNMYKSILVTSQDKAPAVIRKAMDKHNLEEEEPEDYELLQILSDDRKLKIPENANVFYAMNSTANYDFVLKKRTFTKGVKVKHGASSTLPRMKQKGLKIAKGIF DIP protein Q12967 original sequence version: 101 DIP protein Q12967 original sequence version: 136 EA5A5CF25AF3D523 ned4l_human E3 ubiquitin-protein ligase NEDD4-like Nedd4-2 NEDD4.2 NEDD4L KIAA0439 NEDL3 HECT-type E3 ubiquitin transferase NED4L protein protein human Homo sapiens Human MATGLGEPVYGLSEDEGESRILRVKVVSGIDLAKKDIFGASDPYVKLSLYVADENRELALVQTKTIKKTLNPKWNEEFYFRVNPSNHRLLFEVFDENRLTRDDFLGQVDVPLSHLPTEDPTMERPYTFKDFLLRPRSHKSRVKGFLRLKMAYMPKNGGQDEENSDQRDDMEHGWEVVDSNDSASQHQEELPPPPLPPGWEEKVDNLGRTYYVNHNNRTTQWHRPSLMDVSSESDNNIRQINQEAAHRRFRSRRHISEDLEPEPSEGGDVPEPWETISEEVNIAGDSLGLALPPPPASPGSRTSPQELSEELSRRLQITPDSNGEQFSSLIQREPSSRLRSCSVTDAVAEQGHLPPPSAPAGRARSSTVTGGEEPTPSVAYVHTTPGLPSGWEERKDAKGRTYYVNHNNRTTTWTRPIMQLAEDGASGSATNSNNHLIEPQIRRPRSLSSPTVTLSAPLEGAKDSPVRRAVKDTLSNPQSPQPSPYNSPKPQHKVTQSFLPPGWEMRIAPNGRPFFIDHNTKTTTWEDPRLKFPVHMRSKTSLNPNDLGPLPPGWEERIHLDGRTFYIDHNSKITQWEDPRLQNPAITGPAVPYSREFKQKYDYFRKKLKKPADIPNRFEMKLHRNNIFEESYRRIMSVKRPDVLKARLWIEFESEKGLDYGGVAREWFFLLSKEMFNPYYGLFEYSATDNYTLQINPNSGLCNEDHLSYFTFIGRVAGLAVFHGKLLDGFFIRPFYKMMLGKQITLNDMESVDSEYYNSLKWILENDPTELDLMFCIDEENFGQTYQVDLKPNGSEIMVTNENKREYIDLVIQWRFVNRVQKQMNAFLEGFTELLPIDLIKIFDENELELLMCGLGDVDVNDWRQHSIYKNGYCPNHPVIQWFWKAVLLMDAEKRIRLLQFVTGTSRVPMNGFAELYGSNGPQLFTIEQWGSPEKLPRAHTCFNRLDLPPYETFEDLREKLLMAVENAQGFEGVD DIP protein Q96PU5 original sequence version: 83 2C958625B4A1AB3F nedd4_human E3 ubiquitin-protein ligase NEDD4 Neural precursor cell expressed developmentally down-regulated protein 4 NEDD4 KIAA0093 NEDD4-1 Cell proliferation-inducing gene 53 protein PIG53 HECT-type E3 ubiquitin transferase NEDD4 protein protein human Homo sapiens Human MAQSLRLHFAARRSNTYPLSETSGDDLDSHVHMCFKRPTRISTSNVVQMKLTPRQTALAPLIKENVQSQERSSVPSSENVNKKSSCLQISLQPTRYSGYLQSSNVLADSDDASFTCILKDGIYSSAVVDNELNAVNDGHLVSSPAICSGSLSNFSTSDNGSYSSNGSDFGSCASITSGGSYTNSVISDSSSYTFPPSDDTFLGGNLPSDSTSNRSVPNRNTTPCEIFSRSTSTDPFVQDDLEHGLEIMKLPVSRNTKIPLKRYSSLVIFPRSPSTTRPTSPTSLCTLLSKGSYQTSHQFIISPSEIAHNEDGTSAKGFLSTAVNGLRLSKTICTPGEVRDIRPLHRKGSLQKKIVLSNNTPRQTVCEKSSEGYSCVSVHFTQRKAATLDCETTNGDCKPEMSEIKLNSDSEYIKLMHRTSACLPSSQNVDCQININGELERPHSQMNKNHGILRRSISLGGAYPNISCLSSLKHNCSKGGPSQLLIKFASGNEGKVDNLSRDSNRDCTNELSNSCKTRDDFLGQVDVPLYPLPTENPRLERPYTFKDFVLHPRSHKSRVKGYLRLKMTYLPKTSGSEDDNAEQAEELEPGWVVLDQPDAACHLQQQQEPSPLPPGWEERQDILGRTYYVNHESRRTQWKRPTPQDNLTDAENGNIQLQAQRAFTTRRQISEETESVDNRESSENWEIIREDEATMYSNQAFPSPPPSSNLDVPTHLAEELNARLTIFGNSAVSQPASSSNHSSRRGSLQAYTFEEQPTLPVLLPTSSGLPPGWEEKQDERGRSYYVDHNSRTTTWTKPTVQATVETSQLTSSQSSAGPQSQASTSDSGQQVTQPSEIEQGFLPKGWEVRHAPNGRPFFIDHNTKTTTWEDPRLKIPAHLRGKTSLDTSNDLGPLPPGWEERTHTDGRIFYINHNIKRTQWEDPRLENVAITGPAVPYSRDYKRKYEFFRRKLKKQNDIPNKFEMKLRRATVLEDSYRRIMGVKRADFLKARLWIEFDGEKGLDYGGVAREWFFLISKEMFNPYYGLFEYSATDNYTLQINPNSGLCNEDHLSYFKFIGRVAGMAVYHGKLLDGFFIRPFYKMMLHKPITLHDMESVDSEYYNSLRWILENDPTELDLRFIIDEELFGQTHQHELKNGGSEIVVTNKNKKEYIYLVIQWRFVNRIQKQMAAFKEGFFELIPQDLIKIFDENELELLMCGLGDVDVNDWREHTKYKNGYSANHQVIQWFWKAVLMMDSEKRIRLLQFVTGTSRVPMNGFAELYGSNGPQSFTVEQWGTPEKLPRAHTCFNRLDLPPYESFEELWDKLQMAIENTQGFDGVD DIP protein P46934 original sequence version: 76 D56EBBC50A34F13B itch_human E3 ubiquitin-protein ligase Itchy homolog HECT-type E3 ubiquitin transferase Itchy homolog Atrophin-1-interacting protein 4 NFE2-associated polypeptide 1 ITCH protein protein human Homo sapiens Human MSDSGSQLGSMGSLTMKSQLQITVISAKLKENKKNWFGPSPYVEVTVDGQSKKTEKCNNTNSPKWKQPLTVIVTPVSKLHFRVWSHQTLKSDVLLGTAALDIYETLKSNNMKLEEVVVTLQLGGDKEPTETIGDLSICLDGLQLESEVVTNGETTCSENGVSLCLPRLECNSAISAHCNLCLPGLSDSPISASRVAGFTGASQNDDGSRSKDETRVSTNGSDDPEDAGAGENRRVSGNNSPSLSNGGFKPSRPPRPSRPPPPTPRRPASVNGSPSATSESDGSSTGSLPPTNTNTNTSEGATSGLIIPLTISGGSGPRPLNPVTQAPLPPGWEQRVDQHGRVYYVDHVEKRTTWDRPEPLPPGWERRVDNMGRIYYVDHFTRTTTWQRPTLESVRNYEQWQLQRSQLQGAMQQFNQRFIYGNQDLFATSQSKEFDPLGPLPPGWEKRTDSNGRVYFVNHNTRITQWEDPRSQGQLNEKPLPEGWEMRFTVDGIPYFVDHNRRTTTYIDPRTGKSALDNGPQIAYVRDFKAKVQYFRFWCQQLAMPQHIKITVTRKTLFEDSFQQIMSFSPQDLRRRLWVIFPGEEGLDYGGVAREWFFLLSHEVLNPMYCLFEYAGKDNYCLQINPASYINPDHLKYFRFIGRFIAMALFHGKFIDTGFSLPFYKRILNKPVGLKDLESIDPEFYNSLIWVKENNIEECDLEMYFSVDKEILGEIKSHDLKPNGGNILVTEENKEEYIRMVAEWRLSRGVEEQTQAFFEGFNEILPQQYLQYFDAKELEVLLCGMQEIDLNDWQRHAIYRHYARTSKQIMWFWQFVKEIDNEKRMRLLQFVTGTCRLPVGGFADLMGSNGPQKFCIEKVGKENWLPRSHTCFNRLDLPPYKSYEQLKEKLLFAIEETEGFGQE DIP protein Q96J02 original sequence version: 130 DIP protein Q96J02-1 original sequence version: 125 6777A2043C7B67BC rap1a_human Ras-related protein Rap-1A GTP-binding protein smg p21A Ras-related protein Krev-1 C21KG G-22K RAP1A KREV1 protein protein human Homo sapiens Human MREYKLVVLGSGGVGKSALTVQFVQGIFVEKYDPTIEDSYRKQVEVDCQQCMLEILDTAGTEQFTAMRDLYMKNGQGFALVYSITAQSTFNDLQDLREQILRVKDTEDVPMILVGNKCDLEDERVVGKEQGQNLARQWCNCAFLESSAKSKINVNEIFYDLVRQINRKTPVEKKKPKKKSCLLL DIP protein P62834 original sequence version: 37 DIP protein P62834 original sequence version: 93 42C39290C98E0A92 arrd1_human Arrestin domain-containing protein 1 Alpha-arrestin 1 ARRDC1 protein protein human Homo sapiens Human MGRVQLFEISLSHGRVVYSPGEPLAGTVRVRLGAPLPFRAIRVTCIGSCGVSNKANDTAWVVEEGYFNSSLSLADKGSLPAGEHSFPFQFLLPATAPTSFEGPFGKIVHQVRAAIHTPRFSKDHKCSLVFYILSPLNLNSIPDIEQPNVASATKKFSYKLVKTGSVVLTASTDLRGYVVGQALQLHADVENQSGKDTSPVVASLLQKVSYKAKRWIHDVRTIAEVEGAGVKAWRRAQWHEQILVPALPQSALPGCSLIHIDYYLQVSLKAPEATVTLPVFIGNIAVNHAPVSPRPGLGLPPGAPPLVVPSAPPQEEAEAEAAAGGPHFLDPVFLSTKSHSQRQPLLATLSSVPGAPEPCPQDGSPASHPLHPPLCISTGATVPYFAEGSGGPVPTTSTLILPPEYSSWGYPYEAPPSYEQSCGGVEPSLTPES 5B52A79EE27B5DDB arrd4_human Arrestin domain-containing protein 4 ARRDC4 protein protein human Homo sapiens Human MGGEAGCAAAVGAEGRVKSLGLVFEDERKGCYSSGETVAGHVLLEASEPVALRALRLEAQGRATAAWGPSTCPRASASTAALAVFSEVEYLNVRLSLREPPAGEGIILLQPGKHEFPFRFQLPSEPLVTSFTGKYGSIQYCVRAVLERPKVPDQSVKRELQVVSHVDVNTPALLTPVLKTQEKMVGCWFFTSGPVSLSAKIERKGYCNGEAIPIYAEIENCSSRLIVPKAAIFQTQTYLASGKTKTIRHMVANVRGNHIASGSTDTWNGKTLKIPPVTPSILDCCIIRVDYSLAVYIHIPGAKKLMLELPLVIGTIPYNGFGSRNSSIASQFSMDMSWLTLTLPEQPEAPPNYADVVSEEEFSRHIPPYPQPPNCEGEVCCPVFACIQEFRFQPPPLYSEVDPHPSDVEESQPVSFIL 32E37F8AD2450074 magi2_human Membrane-associated guanylate kinase, WW and PDZ domain-containing protein 2 MAGI2 KIAA0705 ACVRINP1 Membrane-associated guanylate kinase inverted 2 Atrophin-1-interacting protein 1 Atrophin-1-interacting protein A AIP1 protein protein human Homo sapiens Human MSKSLKKKSHWTSKVHESVIGRNPEGQLGFELKGGAENGQFPYLGEVKPGKVAYESGSKLVSEELLLEVNETPVAGLTIRDVLAVIKHCKDPLRLKCVKQGGIVDKDLRHYLNLRFQKGSVDHELQQIIRDNLYLRTVPCTTRPHKEGEVPGVDYIFITVEDFMELEKSGALLESGTYEDNYYGTPKPPAEPAPLLLNVTDQILPGATPSAEGKRKRNKSVSNMEKASIEPPEEEEEERPVVNGNGVVVTPESSEHEDKSAGASGEMPSQPYPAPVYSQPEELKEQMDDTKPTKPEDNEEPDPLPDNWEMAYTEKGEVYFIDHNTKTTSWLDPRLAKKAKPPEECKENELPYGWEKIDDPIYGTYYVDHINRRTQFENPVLEAKRKLQQHNMPHTELGTKPLQAPGFREKPLFTRDASQLKGTFLSTTLKKSNMGFGFTIIGGDEPDEFLQVKSVIPDGPAAQDGKMETGDVIVYINEVCVLGHTHADVVKLFQSVPIGQSVNLVLCRGYPLPFDPEDPANSMVPPLAIMERPPPVMVNGRHNYETYLEYISRTSQSVPDITDRPPHSLHSMPTDGQLDGTYPPPVHDDNVSMASSGATQAELMTLTIVKGAQGFGFTIADSPTGQRVKQILDIQGCPGLCEGDLIVEINQQNVQNLSHTEVVDILKDCPIGSETSLIIHRGGFFSPWKTPKPIMDRWENQGSPQTSLSAPAIPQNLPFPPALHRSSFPDSTEAFDPRKPDPYELYEKSRAIYESRQQVPPRTSFRMDSSGPDYKELDVHLRRMESGFGFRILGGDEPGQPILIGAVIAMGSADRDGRLHPGDELVYVDGIPVAGKTHRYVIDLMHHAARNGQVNLTVRRKVLCGGEPCPENGRSPGSVSTHHSSPRSDYATYTNSNHAAPSSNASPPEGFASHSLQTSDVVIHRKENEGFGFVIISSLNRPESGSTITVPHKIGRIIDGSPADRCAKLKVGDRILAVNGQSIINMPHADIVKLIKDAGLSVTLRIIPQEELNSPTSAPSSEKQSPMAQQSPLAQQSPLAQPSPATPNSPIAQPAPPQPLQLQGHENSYRSEVKARQDVKPDIRQPPFTDYRQPPLDYRQPPGGDYQQPPPLDYRQPPLLDYRQHSPDTRQYPLSDYRQPQDFDYFTVDMEKGAKGFGFSIRGGREYKMDLYVLRLAEDGPAIRNGRMRVGDQIIEINGESTRDMTHARAIELIKSGGRRVRLLLKRGTGQVPEYDEPAPWSSPAAAAPGLPEVGVSLDDGLAPFSPSHPAPPSDPSHQISPGPTWDIKREHDVRKPKELSACGQKKQRLGEQRERSASPQRAARPRLEEAPGGQGRPEAGRPASEARAPGLAAADAADAARAGGKEAPRAAAGSELCRREGPGAAPAFAGPGGGGSGALEAEGRAGARAGPRPGPRPPGGAPARKAAVAPGPWKVPGSDKLPSVLKPGASAASR 93E170D070A70A9C v2r_human Vasopressin V2 receptor AVPR V2 Antidiuretic hormone receptor Renal-type arginine vasopressin receptor AVPR2 ADHR DIR DIR3 V2R protein protein human Homo sapiens Human MLMASTTSAVPGHPSLPSLPSNSSQERPLDTRDPLLARAELALLSIVFVAVALSNGLVLAALARRGRRGHWAPIHVFIGHLCLADLAVALFQVLPQLAWKATDRFRGPDALCRAVKYLQMVGMYASSYMILAMTLDRHRAICRPMLAYRHGSGAHWNRPVLVAWAFSLLLSLPQLFIFAQRNVEGGSGVTDCWACFAEPWGRRTYVTWIALMVFVAPTLGIAACQVLIFREIHASLVPGPSERPGGRRRGRRTGSPGEGAHVSAAVAKTVRMTLVIVVVYVLCWAPFFLVQLWAAWDPEAPLEGAPFVLLMLLASLNSCTNPWIYASFSSSVSSELRSLLCCARGRTPPSLGPQDESCTTASSSLAKDTSS 1F87D20A3C0ECB0D adrb2_human Beta-2 adrenergic receptor Beta-2 adrenoreceptor ADRB2 ADRB2R B2AR protein protein human Homo sapiens Human MGQPGNGSAFLLAPNGSHAPDHDVTQERDEVWVVGMGIVMSLIVLAIVFGNVLVITAIAKFERLQTVTNYFITSLACADLVMGLAVVPFGAAHILMKMWTFGNFWCEFWTSIDVLCVTASIETLCVIAVDRYFAITSPFKYQSLLTKNKARVIILMVWIVSGLTSFLPIQMHWYRATHQEAINCYANETCCDFFTNQAYAIASSIVSFYVPLVIMVFVYSRVFQEAKRQLQKIDKSEGRFHVQNLSQVEQDGRTGHGLRRSSKFCLKEHKALKTLGIIMGTFTLCWLPFFIVNIVHVIQDNLIRKEVYILLNWIGYVNSGFNPLIYCRSPDFRIAFQELLCLRRSSLKAYGNGYSSNGNTGEQSGYHVEQEKENKLLCEDLPGTEDFVGHQGTVPSDNIDSQGRNCSTNDSLL DIP protein P07550 original sequence version: 180 DIP protein P07550 original sequence version: 144 408C22731C6EDFBE wwp2_human NEDD4-like E3 ubiquitin-protein ligase WWP2 WW domain-containing protein 2 Atrophin-1-interacting protein 2 WWP2 HECT-type E3 ubiquitin transferase WWP2 protein protein human Homo sapiens Human MASASSSRAGVALPFEKSQLTLKVVSAKPKVHNRQPRINSYVEVAVDGLPSETKKTGKRIGSSELLWNEIIILNVTAQSHLDLKVWSCHTLRNELLGTASVNLSNVLKNNGGKMENMQLTLNLQTENKGSVVSGGELTIFLDGPTVDLGNVPNGSALTDGSQLPSRDSSGTAVAPENRHQPPSTNCFGGRSRTHRHSGASARTTPATGEQSPGARSRHRQPVKNSGHSGLANGTVNDEPTTATDPEEPSVVGVTSPPAAPLSVTPNPNTTSLPAPATPAEGEEPSTSGTQQLPAAAQAPDALPAGWEQRELPNGRVYYVDHNTKTTTWERPLPPGWEKRTDPRGRFYYVDHNTRTTTWQRPTAEYVRNYEQWQSQRNQLQGAMQHFSQRFLYQSSSASTDHDPLGPLPPGWEKRQDNGRVYYVNHNTRTTQWEDPRTQGMIQEPALPPGWEMKYTSEGVRYFVDHNTRTTTFKDPRPGFESGTKQGSPGAYDRSFRWKYHQFRFLCHSNALPSHVKISVSRQTLFEDSFQQIMNMKPYDLRRRLYIIMRGEEGLDYGGIAREWFFLLSHEVLNPMYCLFEYAGKNNYCLQINPASSINPDHLTYFRFIGRFIAMALYHGKFIDTGFTLPFYKRMLNKRPTLKDLESIDPEFYNSIVWIKENNLEECGLELYFIQDMEILGKVTTHELKEGGESIRVTEENKEEYIMLLTDWRFTRGVEEQTKAFLDGFNEVAPLEWLRYFDEKELELMLCGMQEIDMSDWQKSTIYRHYTKNSKQIQWFWQVVKEMDNEKRIRLLQFVTGTCRLPVGGFAELIGSNGPQKFCIDKVGKETWLPRSHTCFNRLDLPPYKSYEQLREKLLYAIEETEGFGQE FCCD75CBA61F2204 arrd3_human Arrestin domain-containing protein 3 KIAA1376 TBP-2-like inducible membrane protein ARRDC3 protein protein human Homo sapiens Human MVLGKVKSLTISFDCLNDSNVPVYSSGDTVSGRVNLEVTGEIRVKSLKIHARGHAKVRWTESRNAGSNTAYTQNYTEEVEYFNHKDILIGHERDDDNSEEGFHTIHSGRHEYAFSFELPQTPLATSFEGRHGSVRYWVKAELHRPWLLPVKLKKEFTVFEHIDINTPSLLSPQAGTKEKTLCCWFCTSGPISLSAKIERKGYTPGESIQIFAEIENCSSRMVVPKAAIYQTQAFYAKGKMKEVKQLVANLRGESLSSGKTETWNGKLLKIPPVSPSILDCSIIRVEYSLMVYVDIPGAMDLFLNLPLVIGTIPLHPFGSRTSSVSSQCSMNMNWLSLSLPERPEAPPSYAEVVTEEQRRNNLAPVSACDDFERALQGPLFAYIQEFRFLPPPLYSEIDPNPDQSADDRPSCPSR 9B02190E1BA90B1D adrb2-arrdc4-1 3 14 unspecified role unspecified role neutral component neutral component n_terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 16 tag fluorescence tag visualisation by fluorescence tag fluorescence immunocytochemistry immunocytochemistry unspecified role unspecified role neutral component neutral component c_terminus mcherry mcherry fluorescent protein tag mcherry c-term range C-terminal range c-term range c-term range C-terminal range c-term range colocalization colocalization 2A CHEBI:64317:isoproterenol (Isoprenaline) - a non-selective beta-adrenoreceptor agonist that is structurally similar to adrenaline. I have added "endocytosis" as an XRef - although I cannot see from this image given that they can deduce this ? - the "data not shown"? - please remove if you think inappropriate... adrb2-arrdc3-2 4 14 unspecified role unspecified role bait bait n_terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 16 unspecified role unspecified role prey prey c_terminus gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range physical association physical association 2B CHEBI:64317:isoproterenol (Isoprenaline) - a non-selective beta-adrenoreceptor agonist that is structurally similar to adrenaline. Clathrin (heavy chains) and HGS were also detected but have not been annotated as specific interactors because they were also immunoprecipitated by the empty vector. adrb2-arrdc3-3 4 13 unspecified role unspecified role bait bait n_terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range c_terminus v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 11 unspecified role unspecified role prey prey c_terminus mcherry mcherry fluorescent protein tag mcherry c-term range C-terminal range c-term range c-term range C-terminal range c-term range physical association physical association 2C Clathrin (heavy chains) and HGS were also detected but have not been annotated as specific interactors because they were also immunoprecipitated with the empty vector. AVP-Arginine-vasopressin -UniProt ID:PRO_0000020515 The ARRDC4 tag has been entered as "mcherry" despite the fact the image text refers to pcDNA3-"ARRDC4-FLAG"; figure 3 specifically mentions that an mcherry tag was used in figure 2C. avpr2-arrdc4-1 2 13 unspecified role unspecified role prey prey n_terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range c_terminus v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 11 unspecified role unspecified role bait bait c_terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range physical association physical association 3A AVP-Arginine-vasopressin -UniProt ID:PRO_0000020515 adrb2-arrdc3-4 2 14 unspecified role unspecified role prey prey n_terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range c_terminus v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 16 unspecified role unspecified role bait bait c_terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range physical association physical association 3A CHEBI:64317:isoproterenol (Isoprenaline) - a non-selective beta-adrenoreceptor agonist that is structurally similar to adrenaline. arrdc1-itch-2 1 10 unspecified role unspecified role bait bait c_terminal_tail_Region sufficient to bind sufficient binding region sufficient to bind range range certain certain sequence position certain 8 unspecified role unspecified role prey prey c_terminal_ww_domains sufficient to bind sufficient binding region sufficient to bind range range range range 49 47 physical association physical association 4A arrdc3-itch-2 1 8 unspecified role unspecified role prey prey c_terminal_ww_domains sufficient to bind sufficient binding region sufficient to bind range range range range 16 unspecified role unspecified role bait bait c_terminal_tail_Region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 54 52 physical association physical association 4Ai arrdc1-wwp2-1 1 16 unspecified role unspecified role bait bait c_terminal_tail_Region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 15 unspecified role unspecified role prey prey c_terminal_ww_domains sufficient to bind sufficient binding region sufficient to bind range range range range 57 59 physical association physical association 4A-3 arrdc3-wwp2-1 1 6 unspecified role unspecified role prey prey c_terminal_ww_domains sufficient to bind sufficient binding region sufficient to bind range range range range 16 unspecified role unspecified role bait bait c_terminal_tail_Region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 64 62 physical association physical association 4A-4 arrdc3-nedd4l-1 1 16 unspecified role unspecified role bait bait c_terminal_tail_Region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 12 unspecified role unspecified role prey prey ww_domains sufficient to bind sufficient binding region sufficient to bind range range range range 67 69 physical association physical association 4A-5 adrb2-arrdc3-5 3 7 unspecified role unspecified role neutral component neutral component gfp_or_(n_terminal)_myc_tag tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 16 tag fluorescence tag visualisation by fluorescence tag fluorescence immunocytochemistry immunocytochemistry unspecified role unspecified role neutral component neutral component c_terminus mcherry mcherry fluorescent protein tag mcherry c-term range C-terminal range c-term range c-term range C-terminal range c-term range colocalization colocalization 4B CHEBI:64317:isoproterenol (Isoprenaline) - a non-selective beta-adrenoreceptor agonist that is structurally similar to adrenaline. It is not clear if NEDD4 has a GFP or a myc (N-terminal) tag. strictly speaking this interaction is not seen elsewhere so should not have been added- but I had added it before I noticed that everywhere else used NEDD4L. Left in as the text says it is a NEDD4L "family member" adrb2-arrdc3-6 4 15 unspecified role unspecified role prey prey 6 unspecified role unspecified role prey prey 8 unspecified role unspecified role prey prey 16 unspecified role unspecified role bait bait c_terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range Q96B67:p.[Pro347Ala;Tyr349Ala;Pro392Ala;Tyr394Ala] mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain P A certain certain sequence position certain certain certain sequence position certain Y A certain certain sequence position certain certain certain sequence position certain P A certain certain sequence position certain certain certain sequence position certain Y A Wed May 02 19:54:40 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'pro347ala,tyr349ala,pro392ala,tyr394ala' Tue Jun 30 18:36:00 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Pro347Ala;Tyr349Ala;Pro392Ala;Tyr394Ala]' association association 4C PY motif mutagenesis revealed that aArr-E3 interactions were PY-dependent. I have gone with what the authors state here- rather than add a mutated bait in a separate (very weak) interaction arrdc3-itch-1 4 15 unspecified role unspecified role prey prey 6 unspecified role unspecified role prey prey 8 unspecified role unspecified role prey prey 11 unspecified role unspecified role bait bait c_terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range Q8NCT1:p.[Pro351Ala;Tyr353Ala;Pro396Ala;Tyr398Ala] mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain P A certain certain sequence position certain certain certain sequence position certain Y A certain certain sequence position certain certain certain sequence position certain P A certain certain sequence position certain certain certain sequence position certain Y A Wed May 02 15:57:53 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'pro351ala,tyr353ala,pro396ala,tyr398ala' Tue Jun 30 20:04:47 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Pro351Ala;Tyr353Ala;Pro396Ala;Tyr398Ala]' association association 4D PY motif mutagenesis revealed that aArr-E3 interactions were PY-dependent. I have gone with what the authors state here- rather than add a mutated bait in a separate (very weak) interaction adrb2-arrdc3-7 4 8 unspecified role unspecified role prey prey 14 unspecified role unspecified role bait bait n_terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range c_terminus v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 16 unspecified role unspecified role prey prey association association 5C CHEBI:64317:isoproterenol (Isoprenaline) - a non-selective beta-adrenoreceptor agonist that is structurally similar to adrenaline. I have not added binary interaction with ITCH alone as ARRDC3 knockdown does not look to have been very successful. rap1a-ralgds-1 1 9 unspecified role unspecified role bait bait 5 unspecified role unspecified role prey prey physical association physical association 4A Strong positive control. A mutant Prey plasmid pEXP22/RALGDS-m1 with an unknown mutation acts as a weak positive control. arrdc1-wwp2-2 1 10 unspecified role unspecified role bait bait c_terminal_tail_Region sufficient to bind sufficient binding region sufficient to bind range range certain certain sequence position certain 15 unspecified role unspecified role prey prey c_terminal_ww_domains sufficient to bind sufficient binding region sufficient to bind range range range range 102 100 physical association physical association 4A-2i