IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Atg18 function in autophagy is regulated by specific sites within its β-propeller. Atg18 function in autophagy is regulated by specific sites within its β-propeller. Journal of cell science 2013 imex curation Rieter E., Vinke F., Bakula D., Cebollero E., Ungermann C., Proikas-Cezanne T., Reggiori F. ATg8-18 colocalisation not added as I think Atg8 is just a location marker. glycerol gradient not added as no pre-purification F.Reggiori@umcutrecht.nl 22-Jun-2016: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro filter binding filter binding Filter overlay assay Filter overlay assay antibody detection identification by antibody antibody detection Rieter E., Vinke F., Bakula D., Cebollero E., Ungermann C., Proikas-Cezanne T., Reggiori F. ATg8-18 colocalisation not added as I think Atg8 is just a location marker. glycerol gradient not added as no pre-purification F.Reggiori@umcutrecht.nl Accepted 2016-MAY-26 AT 13:43 BST AT 13:43 BST by MGT Journal of cell science 2013 Only protein-protein interactions imex curation imex curation Atg18 function in autophagy is regulated by specific sites within its β-propeller. Atg18 function in autophagy is regulated by specific sites within its β-propeller. Journal of cell science 2013 imex curation Rieter E., Vinke F., Bakula D., Cebollero E., Ungermann C., Proikas-Cezanne T., Reggiori F. ATg8-18 colocalisation not added as I think Atg8 is just a location marker. glycerol gradient not added as no pre-purification F.Reggiori@umcutrecht.nl 22-Jun-2016: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro affinity chrom affinity chromatography technology Affinity purification Affinity purification affinity chrom western blot western blot Immuno blot Immuno blot Rieter E., Vinke F., Bakula D., Cebollero E., Ungermann C., Proikas-Cezanne T., Reggiori F. ATg8-18 colocalisation not added as I think Atg8 is just a location marker. glycerol gradient not added as no pre-purification F.Reggiori@umcutrecht.nl Accepted 2016-MAY-26 AT 13:43 BST AT 13:43 BST by MGT Journal of cell science 2013 Only protein-protein interactions imex curation imex curation Not changed, as discussed; Not changed, as discussed. The comment is there, so I'm a bit confused by that one.; Participant detection cannot be Western Blot by our definition because we describe a gel separation phase which is missing here. I think the mutant is correct, Phe is not in bold in Fig. 2A so remains remains Phe. All else amended.; Again, I think the mutant is correct. Atg18 function in autophagy is regulated by specific sites within its β-propeller. Atg18 function in autophagy is regulated by specific sites within its β-propeller. Journal of cell science 2013 imex curation Rieter E., Vinke F., Bakula D., Cebollero E., Ungermann C., Proikas-Cezanne T., Reggiori F. ATg8-18 colocalisation not added as I think Atg8 is just a location marker. glycerol gradient not added as no pre-purification F.Reggiori@umcutrecht.nl 22-Jun-2016: Contacted by IntAct-Help. Only protein-protein interactions imex curation yeast Saccharomyces cerevisiae Baker's yeast bifc bimolecular fluorescence complementation bifc tag fluorescence tag visualisation by fluorescence tag fluorescence Rieter E., Vinke F., Bakula D., Cebollero E., Ungermann C., Proikas-Cezanne T., Reggiori F. ATg8-18 colocalisation not added as I think Atg8 is just a location marker. glycerol gradient not added as no pre-purification F.Reggiori@umcutrecht.nl Accepted 2016-MAY-26 AT 13:43 BST AT 13:43 BST by MGT Journal of cell science 2013 Only protein-protein interactions imex curation imex curation Not changed, as discussed; Not changed, as discussed. The comment is there, so I'm a bit confused by that one.; Participant detection cannot be Western Blot by our definition because we describe a gel separation phase which is missing here. I think the mutant is correct, Phe is not in bold in Fig. 2A so remains remains Phe. All else amended.; Again, I think the mutant is correct. Atg18 function in autophagy is regulated by specific sites within its β-propeller. Atg18 function in autophagy is regulated by specific sites within its β-propeller. Journal of cell science 2013 imex curation Rieter E., Vinke F., Bakula D., Cebollero E., Ungermann C., Proikas-Cezanne T., Reggiori F. ATg8-18 colocalisation not added as I think Atg8 is just a location marker. glycerol gradient not added as no pre-purification F.Reggiori@umcutrecht.nl 22-Jun-2016: Contacted by IntAct-Help. Only protein-protein interactions imex curation yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Rieter E., Vinke F., Bakula D., Cebollero E., Ungermann C., Proikas-Cezanne T., Reggiori F. ATg8-18 colocalisation not added as I think Atg8 is just a location marker. glycerol gradient not added as no pre-purification F.Reggiori@umcutrecht.nl Accepted 2016-MAY-26 AT 13:43 BST AT 13:43 BST by MGT Journal of cell science 2013 Only protein-protein interactions imex curation imex curation Not changed, as discussed; Not changed, as discussed. The comment is there, so I'm a bit confused by that one.; Participant detection cannot be Western Blot by our definition because we describe a gel separation phase which is missing here. I think the mutant is correct, Phe is not in bold in Fig. 2A so remains remains Phe. All else amended.; Again, I think the mutant is correct. atg2_yeast Autophagy-related protein 2 Sporulation-specific protein 72 YNL242W N1106 ATG2 APG2 AUT8 SPO72 protein protein yeast Saccharomyces cerevisiae Baker's yeast MAFWLPQNIQKRLLLYVLQQISLFSNIDLSNLDVSIGSKSHFSFHDVNLSLDDLNIPNVQINEGIVDELVLKLTVSGGVEIDGSGLRFIMTPLYSSGSQELHSDFLVKSIQDLTNSMLQFSDPLTTYNRYKEDDISSSDSSSDLNSNIEASKPAANGSYTLQNMRNKALNVALAKLKIALKDVTIRFIVNDRDPSDNIVEVHLESIQLITTDANLRHINIENITISSIQKQAVPDSPVHPFNNDDLSQSVYLSKMEATSLYMSAMEEQSNEDPSEPQVTQEEQENDKCKESLMEINNLNIAFKGLSSVNDLRMSNIVIDIQDVHLAIHKIVEIKNSTLKNIIDIIVTHLDANESFSCQDSQSPSPDKQEPSALSSVDIKCIYLNLGQDITVILKSFKLEQKENNSLAFSLGSFYSNSSPLTISHKTKPLLTGEQTPQSIALNMGDELDIIISHDGIAHFFKIFQFVSKCMSFYQNKSKGMMPQIASDTKRTVQLTSKAVKLSLKFPYFLLCFQVSPFIYDSNRELYIELVDVFKKLPSRCTKILTMSSITISNLQSPLQLGSYDDTLKEALIYSSVHAIIKEVIFNEEYSGIVQLVEDISAFGKLFTDSKNSECTGKSKSKRGSFLQRSVRVLNSSRFVYKQSLSANFSLKIDSMKLKVSEIIGPQFGSVEALLSNNFFAITDDSQIVYFTKNLKVERKTPSLLEPQEIMSVVLNKAVNEPVLYVHRRANGKLKVIFNNIRIHYYARWLEILKKNIGPDNASSKDEPVSQKLSKKQPTSGFPWELKCLDCSLILHPFRLKSVMVIVLDNLTTGGSSFIPQAKLLSKANTLFLIDDYQNFKIQKDKNWPSLINFYAGQGFSAIGKIDTLNFLINKSDGALLLDCKIEQVGLSLCADSFQTFCQLCIDLKYPQTFPDEEKFRTQLKNPIDVFKDIDCDLFNSAFIRENNHQNDYDSVHLVDSFLDKTHEFNNGARSKLSSQGSYEMDSSSGTATGGILLPHESYLDSAQPKEEDTPPIASKEQERDVDIRGSIDVEKVVIKLFDGYDWKYTRKFIANTVEKLDKELSKAEASSSKSNVPQSEANIFDSIYISANKNNVTDLRRNLDGEIQGVQNSFSDVSKVNLRPSKHYKALIQLNKVHVNLKNYRVDEPDESNSDNSTDVLNRCVVSIYEFEIIDNVPTSTWNKFVTLLKHEPWPHSSPMFLLDLEFIRPIDFLQAVELVMQLNVAPLRLHVDQDTLEFLIRFLGFKDKRFELIDEYPDIVFIQKFSTNSIKLRLDYKPKKVDYAGLRSGQTSELMNFFTLDGSKIILKSVVLYGLNGFDELNNKLKAIWTPDITKKQLPGVLEGLAPVRSFMAIGSGVKTLVTVLMSEYRQEGHLGRSLKKGGNVFLKTTTGDFVKLGVKLTSGTQAILENTEELFGGVGSNGRVYDASKFGSADGADSDTAAVLDLDTLFEEDQLVGSKYSRIRDHEPTAVVIDMSSPGDHNEPTIVSLYADQPLDLPTGLKEAYSSLEKHMHIAYDAVWRAKGQMKDDKRGGPSAAAVYVARAAPVAIIRPLIGATEAVSKTLQGIANQVDKTHNEQINDKYKSNRTDS 54FDDF56297FFF99 54FDDF56297FFF99 1-phosphatidyl-1d-myo-inositol 3,5-bisphosphate 1-phosphatidyl-1D-myo-inositol 3,5-bisphosphates small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) atg18_yeast Autophagy-related protein 18 NMR1 YFR021W ATG18 AUT10 CVT18 SVP1 Cytoplasm to vacuole targeting protein 18 Swollen vacuole phenotype protein 1 Needed for premeiotic replication protein 1 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSDSSPTINFINFNQTGTCISLGTSKGFKIFNCEPFGKFYSEDSGGYAIVEMLFSTSLLALVGIGDQPALSPRRLRIINTKKHSIICEVTFPTSILSVKMNKSRLVVLLQEQIYIYDINTMRLLHTIETNPNPRGLMAMSPSVANSYLVYPSPPKVINSEIKAHATTNNITLSVGGNTETSFKRDQQDAGHSDISDLDQYSSFTKRDDADPTSSNGGNSSIIKNGDVIVFNLETLQPTMVIEAHKGEIAAMAISFDGTLMATASDKGTIIRVFDIETGDKIYQFRRGTYATRIYSISFSEDSQYLAVTGSSKTVHIFKLGHSMSNNKLDSDDSNMEEAAADDSSLDTTSIDALSDEENPTRLAREPYVDASRKTMGRMIRYSSQKLSRRAARTLGQIFPIKVTSLLESSRHFASLKLPVETNSHVMTISSIGSPIDIDTSEYPELFETGNSASTESYHEPVMKMVPIRVVSSDGYLYNFVMDPERGGDCLILSQYSILMD 06B2DFAF842AE933 06B2DFAF842AE933 atg18-atg2-8 4 7 unspecified role unspecified role prey prey loop1, loop2 required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain P43601:p.[Glu42_Ser44delinsAlaAlaAla;Tyr47Ala;Thr90_Ser94delinsAlaAlaAlaAlaAla] mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain Y A certain certain sequence position certain certain certain sequence position certain TFPTS AAAAA certain certain sequence position certain certain certain sequence position certain EDS AAA Fri Jul 22 11:13:47 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'glu42ala,asp43ala,ser44ala,tyr47ala,thr90ala,phe91ala,pro92ala,thr93ala,ser94ala' Wed May 02 16:26:59 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu_asp_ser42-44ala_ala_ala,tyr47ala,thr_phe_pro_thr_ser90-94ala_ala_ala_ala_ala' Tue Jun 30 18:28:28 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Glu42_Ser44delinsAlaAlaAla;Tyr47Ala;Thr90_Ser94delinsAlaAlaAlaAlaAla]' 5 unspecified role unspecified role bait bait physical association physical association Fig. 1 atg2-atg18-1 2 7 unspecified role unspecified role prey prey c-terminal myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range P43601:p.Thr90_Ser94delinsAlaAlaAlaAlaAla mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain TFPTS AAAAA Thu Dec 08 14:26:35 GMT 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'thr90ala,phe91ala,pro92ala,thr93ala,ser94ala' Wed May 02 16:55:54 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'thr_phe_pro_thr_ser90-94ala_ala_ala_ala_ala' Tue Jun 30 20:24:59 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Thr90_Ser94delinsAlaAlaAlaAlaAla' region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain P43601:p.[Glu42_Ser44delinsAlaAlaAla;Tyr47Ala;Thr90_Ser94delinsAlaAlaAlaAlaAla] mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain Y A certain certain sequence position certain certain certain sequence position certain TFPTS AAAAA certain certain sequence position certain certain certain sequence position certain EDS AAA Fri Jul 22 11:04:59 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'glu42ala,asp43ala,ser44ala,tyr47ala,thr90ala,phe91ala,pro92ala,thr93ala,ser94ala' Wed May 02 18:02:43 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu_asp_ser42-44ala_ala_ala,tyr47ala,thr_phe_pro_thr_ser90-94ala_ala_ala_ala_ala' Tue Jun 30 16:09:43 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Glu42_Ser44delinsAlaAlaAla;Tyr47Ala;Thr90_Ser94delinsAlaAlaAlaAlaAla]' yeast Saccharomyces cerevisiae Baker's yeast 5 unspecified role unspecified role bait bait c-terminal protein a tag protein a tag protein A protein A c-term range C-terminal range c-term range c-term range C-terminal range c-term range yeast Saccharomyces cerevisiae Baker's yeast physical association physical association Fig. 2A, 7E Interaction was not observed in ATG14-/- cells, which the authors interpreted as indicating that the interaction requires the generation of PtDIns3P. phosphatidylethanolamine-atg18-1 1 7 unspecified role unspecified role prey prey c-terminal myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range P43601:p.Arg285_Arg286delinsAlaAla mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain RR AA Fri Jul 22 10:58:02 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'arg285ala,arg286ala' Wed May 02 19:29:21 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg_arg285-286ala_ala' Tue Jun 30 17:26:01 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg285_Arg286delinsAlaAla' yeast Saccharomyces cerevisiae Baker's yeast 6 unspecified role unspecified role bait bait physical association physical association Fig. 2B atg2-atg18-2 3 7 unspecified role unspecified role neutral component neutral component c-terminal venus-c venus c-terminal fragment c-term range C-terminal range c-term range c-term range C-terminal range c-term range P43601:p.Thr90_Ser94delinsAlaAlaAlaAlaAla mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain TFPTS AAAAA Thu Dec 08 14:31:14 GMT 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'thr90ala,phe91ala,pro92ala,thr93ala,ser94ala' Wed May 02 18:30:59 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'thr_phe_pro_thr_ser90-94ala_ala_ala_ala_ala' Tue Jun 30 16:51:56 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Thr90_Ser94delinsAlaAlaAlaAlaAla' P43601:p.Arg285_Arg286delinsAlaAla mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain RR AA Fri Jul 22 10:55:42 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'arg285ala,arg286ala' Wed May 02 16:12:18 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg_arg285-286ala_ala' Tue Jun 30 16:55:28 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg285_Arg286delinsAlaAla' 5 unspecified role unspecified role neutral component neutral component c-terminal venus-n venus n-terminal fragment n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association Fig. 7A-D Interaction increased in atg3-/- cells, and was complete lost in atg13-/- cells.