NTNU NTNU, Norwegian University of Science and Technology www.ntnu.no/home Gfi1b negatively regulates Rag expression directly and via the repression of FoxO1. Gfi1b negatively regulates Rag expression directly and via the repression of FoxO1. The Journal of experimental medicine 2012 imex curation Schulz D., Vassen L., Chow KT., McWhirter SM., Amin RH., Möröy T., Schlissel MS. mark_schlissel@brown.edu 22-Jun-2016: Contacted by IntAct-Help. Only protein-protein interactions imex curation mouse-pro b cells Mus musculus pro-b-cell pro b cell ch-ip chromatin immunoprecipitation assay ch-ip primer specific pcr primer specific pcr Schulz D., Vassen L., Chow KT., McWhirter SM., Amin RH., Möröy T., Schlissel MS. mark_schlissel@brown.edu Accepted 2016-JUN-01 AT 08:48 BST AT 08:48 BST by ORCHARD The Journal of experimental medicine 2012 Only protein-protein interactions imex curation imex curation gfi1b_mouse Zinc finger protein Gfi-1b Gfi1b Growth factor independent protein 1B protein protein mouse Mus musculus Mouse MPRSFLVKSKKAHTYHQPRAQGDELVWPPAVIPVAKEHSQSASPLLSTPLPSQTLDWNTIKQEREMLLNQSLPKMASAPEGPLVTPQPQDGESPLSESPPFYKPSFSWDTLASSYSHSYTQTPSTMQSAFLERSVRLYGSPLVPSTESPLDFRLRYSPGMDTYHCVKCNKVFSTPHGLEVHVRRSHSGTRPFACDVCGKTFGHAVSLEQHTHVHSQERSFECRMCGKAFKRSSTLSTHLLIHSDTRPYPCQFCGKRFHQKSDMKKHTYIHTGEKPHKCQVCGKAFSQSSNLITHSRKHTGFKPFSCELCTKGFQRKVDLRRHRESQHNLK 6194D1972112E6FC foxo1_mouse_gene Forkhead box protein O1 Foxo1 Fkhr Foxo1a gene gene mouse Mus musculus Mouse rag1_mouse_gene V(D)J recombination-activating protein 1 Rag1 gene gene mouse Mus musculus Mouse gfi1_mouse Zinc finger protein Gfi-1 Gfi1 Gfi-1 Growth factor independent protein 1 protein protein mouse Mus musculus Mouse MPRSFLVKSKKAHSYHQPRSPGPDYSLRLETVPAPGRAEGGAVSAGESKMEPRERLSPDSQLTEAPDRASASPNSCEGSVCDPCSEFEDFWRPPSPSVSPASEKSLCRSLDEAQPYTLPFKPYAWSGLAGSDLRHLVQSYRQCSALERSAGLSLFCERGSEPGRPAARYGPEQAAGGAGAGQPGRCGVAGGATSAAGLGLYGDFAPAAAGLYERPSTAAGRLYQDHGHELHADKSVGVKVESELLCTRLLLGGGSYKCIKCSKVFSTPHGLEVHVRRSHSGTRPFACEMCGKTFGHAVSLEQHKAVHSQERSFDCKICGKSFKRSSTLSTHLLIHSDTRPYPCQYCGKRFHQKSDMKKHTFIHTGEKPHKCQVCGKAFSQSSNLITHSRKHTGFKPFGCDLCGKGFQRKVDLRRHRETQHGLK 63AB726025300B6B rag2_mouse_gene Rag2 gene gene gene mouse Mus musculus Mouse gfi1b-rag1-1 1 2 unspecified role unspecified role bait bait flag-tagged-gfi1b Flag-tagged-Gfi1b flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 4 unspecified role unspecified role prey prey regulatory_region_rag1 Regulatory region Rag1 binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association Figure 8B O70237 decreases expression at RNA level of ENSMUSG00000061311 O70237 decreases the expression at RNA level of ENSMUSG00000061311 as ascertained by quantitative real-time PCR after overexpression of O70237 in AMuLV cells (Figure 1C). gfi1b-rag1-2 1 2 unspecified role unspecified role bait bait flag-tagged-gfi1b Flag-tagged-Gfi1b flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 6 unspecified role unspecified role prey prey regulatory_region_rag2 Regulatory region Rag2 binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association Figure 8B O70237 decreases expression at RNA level of ENSMUSG00000032864 O70237 decreases the expression at RNA level of ENSMUSG00000032864 as ascertained by quantitative real-time PCR after overexpression of O70237 in AMuLV cells (Figure 1C). gfi1b-foxo1_mouse_gene-1 1 2 unspecified role unspecified role bait bait flag-tagged-gfi1b Flag-tagged-Gfi1b flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 3 unspecified role unspecified role prey prey foxo1-regulatory-region Foxo1 regulatory region sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association Figure 10F O70237 decreases expression at RNA level of ENSMUSG00000032864 O70237 decreases expression at RNA level of ENSMUSG00000044167 O70237 decreases the expression at RNA level of ENSMUSG00000044167 as ascertained by quantitative real-time PCR after overexpression of O70237 in AMuLV cells (Figure 10B). gfi1-rag1-3 1 5 unspecified role unspecified role bait bait flag-tagged-gfi1 Flag-tagged-Gfi1 flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 4 unspecified role unspecified role prey prey regulatory_region_rag1 Regulatory region Rag1 binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association Figure 8B gfi1-rag2-1 1 5 unspecified role unspecified role bait bait flag-tagged-gfi1 Flag-tagged-Gfi1 flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 6 unspecified role unspecified role prey prey regulatory_region_rag2 Regulatory region Rag2 binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association Figure 8B