HPIDb Host Pathogen Interaction database http://www.agbase.msstate.edu/hpi/main.html The Brucella TIR-like protein TcpB interacts with the death domain of MyD88. The Brucella TIR-like protein TcpB interacts with the death domain of MyD88. Biochemical and biophysical research communications 2012 imex curation Chaudhary A., Ganguly K., Cabantous S., Waldo GS., Micheva-Viteva SN., Nag K., Hlavacek WS., Tung CS. anuc@u.washington.edu, anuc@uw.edu 22-Jun-2016: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. split luciferase complementation split luciferase complementation predetermined predetermined participant predetermined Chaudhary A., Ganguly K., Cabantous S., Waldo GS., Micheva-Viteva SN., Nag K., Hlavacek WS., Tung CS. anuc@u.washington.edu, anuc@uw.edu Accepted 2016-MAY-25 AT 09:42 BST AT 09:42 BST by MGT Biochemical and biophysical research communications 2012 imex curation tirap_human Toll/interleukin-1 receptor domain-containing adapter protein MyD88 adapter-like protein Adaptor protein Wyatt TIRAP MAL protein protein human Homo sapiens Human MASSTSLPAPGSRPKKPLGKMADWFRQTLLKKPKKRPNSPESTSSDASQPTSQDSPLPPSLSSVTSPSLPPTHASDSGSSRWSKDYDVCVCHSEEDLVAAQDLVSYLEGSTASLRCFLQLRDATPGGAIVSELCQALSSSHCRVLLITPGFLQDPWCKYQMLQALTEAPGAEGCTIPLLSGLSRAAYPPELRFMYYVDGRGPDGGFRQVKEAVMRYLQTLS DIP protein P58753 original sequence version: 119 44A8454D04704540 myd88_human Myeloid differentiation primary response protein MyD88 MYD88 protein protein human Homo sapiens Human MAAGGPGAGSAAPVSSTSSLPLAALNMRVRRRLSLFLNVRTQVAADWTALAEEMDFEYLEIRQLETQADPTGRLLDAWQGRPGASVGRLLELLTKLGRDDVLLELGPSIEEDCQKYILKQQQEEAEKPLQVAAVDSSVPRTAELAGITTLDDPLGHMPERFDAFICYCPSDIQFVQEMIRQLEQTNYRLKLCVSDRDVLPGTCVWSIASELIEKRCRRMVVVVSDDYLQSKECDFQTKFALSLSPGAHQKRLIPIKYKAMKKEFPSILRFITVCDYTNPCTKSWFWTRLAKALSLP CEAE3F6B99524333 q8yf33_brume BMEI1694 protein protein brume Brucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094) MNIHASNLDIEKFRKVYALVTGGATDGERVAAQARARKIAERAGMSLNDAVSQLDSQPKPKPANFFEGFADWMEEKEPGYKAKRAREVVEREQRYASRRAEILKQFGTAKAFLDPTPNERLILKAAEPFIAELGEPYEDACGTWRRPISSFAGVHSHFFNLDDVDPEAIMAIKAAIPFPETICGAFEELKVWDKLNDDRAHFYGHHEYYYELPVELRIELLREVMRTQPVTSWGDLEARFHYKSYAWQRQWIDPKDFDDPEWSRLFDDVRILRALAEKPFREPVQNGRRTNAEKRSAVLSMLDTNPELSDREICRRVGVSPKTVGNWRRRRNDRLSQP A7B8EFDA7DFFE2F8 tcpb-myd88-1 1 3 unspecified role unspecified role neutral component neutral component DD sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain c terminus gaussia-c gaussia-c gaussia-c c-term range C-terminal range c-term range c-term range C-terminal range c-term range 4 unspecified role unspecified role neutral component neutral component n terminus gaussia-n gaussia-n gaussia-n n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association 1B tcpb-tirap-1 1 2 unspecified role unspecified role neutral component neutral component c terminus gaussia-c gaussia-c gaussia-c c-term range C-terminal range c-term range c-term range C-terminal range c-term range 4 unspecified role unspecified role neutral component neutral component n terminus gaussia-n gaussia-n gaussia-n n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association 1B myd88-myd88-9 1 3 inhibitor inhibitor unspecified role unspecified role TIR sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 3 unspecified role unspecified role neutral component neutral component DD sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain gaussia_tag tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 3 unspecified role unspecified role neutral component neutral component gaussia_tag tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined DD sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 20 24 physical association physical association 3B-B1 only MyD88DD and MyD88 can inhibit the homodimerization of full-length MyD88 myd88-myd88-11 1 3 unspecified role unspecified role neutral component neutral component DD sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain gaussia_tag tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 3 inhibitor inhibitor unspecified role unspecified role DD sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 3 unspecified role unspecified role neutral component neutral component DD sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain gaussia_tag tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 32 27 physical association physical association 3B-D1 myd88-myd88-12 1 3 unspecified role unspecified role neutral component neutral component gaussia_tag tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined DD sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 3 unspecified role unspecified role neutral component neutral component DD sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain gaussia_tag tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 3 inhibitor inhibitor unspecified role unspecified role 39 37 physical association physical association 3B-C1 myd88-myd88-4 1 3 unspecified role unspecified role neutral component neutral component n terminus gaussia-n gaussia-n gaussia-n n-term range n-terminal range n-term range n-term range n-terminal range n-term range 3 unspecified role unspecified role neutral component neutral component c terminus gaussia-c gaussia-c gaussia-c c-term range C-terminal range c-term range c-term range C-terminal range c-term range physical association physical association 3A-1 myd88-myd88-5 1 3 unspecified role unspecified role neutral component neutral component n terminus gaussia-n gaussia-n gaussia-n n-term range n-terminal range n-term range n-term range n-terminal range n-term range DD sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 3 unspecified role unspecified role neutral component neutral component c terminus gaussia-c gaussia-c gaussia-c c-term range C-terminal range c-term range c-term range C-terminal range c-term range physical association physical association 3A-2 myd88-myd88-6 1 3 unspecified role unspecified role neutral component neutral component TIR sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain n terminus gaussia-n gaussia-n gaussia-n n-term range n-terminal range n-term range n-term range n-terminal range n-term range 3 unspecified role unspecified role neutral component neutral component c terminus gaussia-c gaussia-c gaussia-c c-term range C-terminal range c-term range c-term range C-terminal range c-term range physical association physical association 3A-3 weak interaction myd88-myd88-7 1 3 unspecified role unspecified role neutral component neutral component n terminus gaussia-n gaussia-n gaussia-n n-term range n-terminal range n-term range n-term range n-terminal range n-term range DD sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 3 unspecified role unspecified role neutral component neutral component DD sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain c terminus gaussia-c gaussia-c gaussia-c c-term range C-terminal range c-term range c-term range C-terminal range c-term range 64 62 physical association physical association 3A-4 myd88-myd88-14 1 3 unspecified role unspecified role neutral component neutral component TIR sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain n terminus gaussia-n gaussia-n gaussia-n n-term range n-terminal range n-term range n-term range n-terminal range n-term range 3 unspecified role unspecified role neutral component neutral component TIR sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain c terminus gaussia-c gaussia-c gaussia-c c-term range C-terminal range c-term range c-term range C-terminal range c-term range 71 68 physical association physical association 3A-5 weak interaction myd88-myd88-15 1 3 unspecified role unspecified role neutral component neutral component n terminus gaussia-n gaussia-n gaussia-n n-term range n-terminal range n-term range n-term range n-terminal range n-term range DD sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 3 unspecified role unspecified role neutral component neutral component TIR sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain c terminus gaussia-c gaussia-c gaussia-c c-term range C-terminal range c-term range c-term range C-terminal range c-term range 76 78 physical association physical association 3A-6 myd88-myd88-16 1 3 unspecified role unspecified role neutral component neutral component n terminus gaussia-n gaussia-n gaussia-n n-term range n-terminal range n-term range n-term range n-terminal range n-term range 3 unspecified role unspecified role neutral component neutral component DD sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain c terminus gaussia-c gaussia-c gaussia-c c-term range C-terminal range c-term range c-term range C-terminal range c-term range physical association physical association 3A-7 myd88-myd88-17 1 3 unspecified role unspecified role neutral component neutral component n terminus gaussia-n gaussia-n gaussia-n n-term range n-terminal range n-term range n-term range n-terminal range n-term range 3 unspecified role unspecified role neutral component neutral component TIR sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain c terminus gaussia-c gaussia-c gaussia-c c-term range C-terminal range c-term range c-term range C-terminal range c-term range physical association physical association 3A-8 myd88-myd88-18 1 3 unspecified role unspecified role neutral component neutral component gaussia_tag tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined TIR sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 3 unspecified role unspecified role neutral component neutral component DD sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain gaussia_tag tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 4 inhibitor inhibitor unspecified role unspecified role 97 95 physical association physical association 3BA-2 TcpB does not significantly perturb homodimerization of full-length MyD88 but it disrupts homo- and heterotypic interactions mediated by the DD of MyD88. only MyD88DD and MyD88 can inhibit the Homodimerization of full-length MyD88 myd88-myd88-19 1 3 unspecified role unspecified role neutral component neutral component gaussia_tag tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined DD sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 3 unspecified role unspecified role neutral component neutral component gaussia_tag tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined DD sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 4 inhibitor inhibitor unspecified role unspecified role 106 103 physical association physical association 3BA-3 TcpB does not significantly perturb homodimerization of full-length MyD88 but it disrupts homo- and heterotypic interactions mediated by the DD of MyD88. only MyD88DD and MyD88 can inhibit the Homodimerization of full-length MyD88 myd88-myd88-20 1 3 unspecified role unspecified role neutral component neutral component TIR sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain gaussia_tag tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 3 unspecified role unspecified role neutral component neutral component gaussia_tag tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 4 stimulator stimulator unspecified role unspecified role physical association physical association 3BA-4 myd88-myd88-10 1 3 inhibitor inhibitor unspecified role unspecified role TIR sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 3 unspecified role unspecified role neutral component neutral component TIR sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain gaussia_tag tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 3 unspecified role unspecified role neutral component neutral component DD sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain gaussia_tag tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 122 119 physical association physical association 3B-B2 myd88-myd88-22 1 3 inhibitor inhibitor unspecified role unspecified role TIR sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 3 unspecified role unspecified role neutral component neutral component gaussia_tag tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 3 unspecified role unspecified role neutral component neutral component gaussia_tag tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined TIR sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 126 131 physical association physical association 3B-B4 myd88-myd88-23 1 3 unspecified role unspecified role neutral component neutral component gaussia_tag tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined TIR sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 3 unspecified role unspecified role neutral component neutral component DD sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain gaussia_tag tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 3 inhibitor inhibitor unspecified role unspecified role 135 137 physical association physical association 3B-C2 myd88-myd88-24 1 3 unspecified role unspecified role neutral component neutral component gaussia_tag tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 3 unspecified role unspecified role neutral component neutral component DD sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain gaussia_tag tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 3 inhibitor inhibitor unspecified role unspecified role physical association physical association 3B-C3 only MyD88DD and MyD88 can inhibit the homodimerization of full-length MyD88 myd88-myd88-25 1 3 unspecified role unspecified role neutral component neutral component gaussia_tag tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 3 unspecified role unspecified role neutral component neutral component gaussia_tag tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 3 inhibitor inhibitor unspecified role unspecified role physical association physical association 3B-C4 only MyD88DD and MyD88 can inhibit the homodimerization of full-length MyD88 myd88-myd88-26 1 3 unspecified role unspecified role neutral component neutral component TIR sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain gaussia_tag tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 3 inhibitor inhibitor unspecified role unspecified role DD sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 3 unspecified role unspecified role neutral component neutral component DD sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain gaussia_tag tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 155 160 physical association physical association 3B-D2 only MyD88DD and MyD88 can inhibit the homodimerization of full-length MyD88 myd88-myd88-27 1 3 unspecified role unspecified role neutral component neutral component gaussia_tag tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 3 inhibitor inhibitor unspecified role unspecified role DD sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 3 unspecified role unspecified role neutral component neutral component DD sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain gaussia_tag tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 3B-D3 only MyD88DD and MyD88 can inhibit the homodimerization of full-length MyD88 myd88-myd88-28 1 3 unspecified role unspecified role neutral component neutral component TIR sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain gaussia_tag tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 3 inhibitor inhibitor unspecified role unspecified role DD sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 3 unspecified role unspecified role neutral component neutral component gaussia_tag tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 3B-D4 myd88-myd88-29 1 3 unspecified role unspecified role neutral component neutral component gaussia_tag tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 3 inhibitor inhibitor unspecified role unspecified role DD sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 3 unspecified role unspecified role neutral component neutral component gaussia_tag tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 3B-D5 only MyD88DD and MyD88 can inhibit the homodimerization of full-length MyD88 myd88-myd88-30 1 3 inhibitor inhibitor unspecified role unspecified role TIR sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 3 unspecified role unspecified role neutral component neutral component gaussia_tag tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 3 unspecified role unspecified role neutral component neutral component DD sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain gaussia_tag tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 3B-B3i