UniProt UniProt http://www.uniprot.org Localization and retention of p90 ribosomal S6 kinase 1 in the nucleus: implications for its function. Localization and retention of p90 ribosomal S6 kinase 1 in the nucleus: implications for its function. Molecular biology of the cell 2012 imex curation Gao X., Chaturvedi D., Patel TB. tpatel7@lumc.edu 11-Apr-2016: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Gao X., Chaturvedi D., Patel TB. cAMP agarose has been used for pull down in serum-starved HeLa cells tpatel7@lumc.edu Accepted 2016-APR-06 AT 09:50 BST AT 09:50 BST by MGT Molecular biology of the cell 2012 imex curation added the xref in the cell line (see also as I cannot find it in atcc etc). cannot see the problem with the host, looks ok to me Localization and retention of p90 ribosomal S6 kinase 1 in the nucleus: implications for its function. Localization and retention of p90 ribosomal S6 kinase 1 in the nucleus: implications for its function. Molecular biology of the cell 2012 imex curation Gao X., Chaturvedi D., Patel TB. tpatel7@lumc.edu 11-Apr-2016: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti bait coip anti bait coimmunoprecipitation anti bait coip anti tag western anti tag western blot anti tag western Gao X., Chaturvedi D., Patel TB. tpatel7@lumc.edu Accepted 2016-APR-06 AT 09:50 BST AT 09:50 BST by MGT Molecular biology of the cell 2012 imex curation added the xref in the cell line (see also as I cannot find it in atcc etc). cannot see the problem with the host, looks ok to me Localization and retention of p90 ribosomal S6 kinase 1 in the nucleus: implications for its function. Localization and retention of p90 ribosomal S6 kinase 1 in the nucleus: implications for its function. Molecular biology of the cell 2012 imex curation Gao X., Chaturvedi D., Patel TB. tpatel7@lumc.edu 11-Apr-2016: Contacted by IntAct-Help. Only protein-protein interactions imex curation mouse-b82l mouse B82L fibroblast cell line Mouse b82l B82L fibroblast cell line anti bait coip anti bait coimmunoprecipitation anti bait coip sequence tag sequence tag identification sequence tag Gao X., Chaturvedi D., Patel TB. tpatel7@lumc.edu Accepted 2016-APR-06 AT 09:50 BST AT 09:50 BST by MGT Molecular biology of the cell 2012 imex curation added the xref in the cell line (see also as I cannot find it in atcc etc). cannot see the problem with the host, looks ok to me Localization and retention of p90 ribosomal S6 kinase 1 in the nucleus: implications for its function. Localization and retention of p90 ribosomal S6 kinase 1 in the nucleus: implications for its function. Molecular biology of the cell 2012 imex curation Gao X., Chaturvedi D., Patel TB. tpatel7@lumc.edu 11-Apr-2016: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Gao X., Chaturvedi D., Patel TB. tpatel7@lumc.edu Accepted 2016-APR-06 AT 09:50 BST AT 09:50 BST by MGT Molecular biology of the cell 2012 imex curation added the xref in the cell line (see also as I cannot find it in atcc etc). cannot see the problem with the host, looks ok to me Localization and retention of p90 ribosomal S6 kinase 1 in the nucleus: implications for its function. Localization and retention of p90 ribosomal S6 kinase 1 in the nucleus: implications for its function. Molecular biology of the cell 2012 imex curation Gao X., Chaturvedi D., Patel TB. tpatel7@lumc.edu 11-Apr-2016: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 fluorescence imaging fluorescence microscopy fluorescence imaging immunostaining immunostaining Gao X., Chaturvedi D., Patel TB. tpatel7@lumc.edu Accepted 2016-APR-06 AT 09:50 BST AT 09:50 BST by MGT Molecular biology of the cell 2012 imex curation added the xref in the cell line (see also as I cannot find it in atcc etc). cannot see the problem with the host, looks ok to me actb_mouse Actin, cytoplasmic 1 Actb Beta-actin protein protein mouse Mus musculus Mouse MDDDIAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTGIVMDSGDGVTHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVALDFEQEMATAASSSSLEKSYELPDGQVITIGNERFRCPEALFQPSFLGMESCGIHETTFNSIMKCDVDIRKDLYANTVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDESGPSIVHRKCF 6AFD05CA94E360E2 ks6a1_rat Ribosomal protein S6 kinase alpha-1 Rps6ka1 Rsk1 90 kDa ribosomal protein S6 kinase 1 Ribosomal S6 kinase 1 MAP kinase-activated protein kinase 1a Mapkapk1a protein protein rat Rattus norvegicus (Rat) Rat MPLAQLKEPWPLMELVPLDPENGQASGEEAGLQPSKDEGILKEISITHHVKAGSEKADPSHFELLKVLGQGSFGKVFLVRKVTRPDNGHLYAMKVLKKATLKVRDRVRTKMERDILADVNHPFVVKLHYAFQTEGKLYLILDFLRGGDLFTRLSKEVMFTEEDVKFYLAELALGLDHLHSLGIIYRDLKPENILLDEEGHIKLTDFGLSKEAIDHEKKAYSFCGTVEYMAPEVVNRQGHTHSADWWSYGVLMFEMLTGSLPFQGKDRKETMTLILKAKLGMPQFLSTEAQSLLRALFKRNPANRLGSGPDGAEEIKRHIFYSTIDWNKLYRREIKPPFKPAVAQPDDTFYFDTEFTSRTPRDSPGIPPSAGAHQLFRGFSFVATGLMEDDSKPRATQAPLHSVVQQLHGKNLVFSDGYIVKETIGVGSYSVCKRCVHKATNMEYAVKVIDKSKRDPSEEIEILLRYGQHPNIITLKDVYDDSKHVYLVTELMRGGELLDKILRQKFFSEREASFVLYTISKTVEYLHSQGVVHRDLKPSNILYVDESGNPECLRICDFGFAKQLRAENGLLMTPCYTANFVAPEVLKRQGYDEGCDIWSLGVLLYTMLAGYTPFANGPSDTPEEILTRISSGKFTLSGGNWNTVSETAKDLVSKMLHVDPHQRLTAKQVLQHPWITQKDKLPQSQLSHQDLQLVKGGMAATYSALSSSKPTPQLKPIESSILAQRRVRKLPSTTL Serine/threonine kinase that may play a role in mediating the growth-factor and stress induced activation of the transcription factor CREB mint 12DEAD1E07140FDE ks6a1_mouse Ribosomal protein S6 kinase alpha-1 90 kDa ribosomal protein S6 kinase 1 Mapkapk1a Ribosomal S6 kinase 1 MAP kinase-activated protein kinase 1a Rps6ka1 Rsk1 protein protein mouse Mus musculus Mouse MPLAQLKEPWPLMELVPLDPENGQTSGEEAGLQPSKDEAILKEISITHHVKAGSEKADPSQFELLKVLGQGSFGKVFLVRKVTRPDSGHLYAMKVLKKATLKVRDRVRTKMERDILADVNHPFVVKLHYAFQTEGKLYLILDFLRGGDLFTRLSKEVMFTEEDVKFYLAELALGLDHLHSLGIIYRDLKPENILLDEEGHIKLTDFGLSKEAIDHEKKAYSFCGTVEYMAPEVVNRQGHTHSADWWSYGVLMGKDRKETMTLILKAKLGMPQFLSTEAQSLLRALFKRNPANRLGSGPDGAEEIKRHIFYSTIDWNKLYRREIKPPFKPAVAQPDDTFYFDTEFTSRTPRDSPGIPPSAGAHQLFRGFSFVATGLMEDDGKPRTTQAPLHSVVQQLHGKNLVFSDGYVVKETIGVGSYSVCKRCVHKATNMEYAVKVIDKSKRDPSEEIEILLRYGQHPNIITLKDVYDDGKHVYLVTELMRGGELLDKILRQKFFSEREASFVLHTISKTVEYLHSQGVVHRDLKPSNILYVDESGNPECLRICDFGFAKQLRAENGLLMTPCYTANFVAPEVLKRQGYDEGCDIWSLGILLYTMLAGYTPFANGPSDTPEEILTRIGSGKFTLSGGNWNTVSETAKDLVSKMLHVDPHQRLTAKQVLQHPWITQKDKLPQSQLSHQDLQLVKGAMAATYSALNSSKPTPQLKPIESSILAQRRVRKLPSTTL A5D8E5E5FDBCE4BF 3',5'-cyclic amp 3',5'-cyclic AMP 3',5'-Cyclic AMP adenosine 3',5'-cyclic monophosphate Adenosine 3',5'-cyclic phosphate Adenosine 3',5'-phosphate ADENOSINE-3',5'-CYCLIC-MONOPHOSPHATE cAMP Cyclic adenylic acid Cyclic AMP adenosine 3',5'-(hydrogen phosphate) small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) akap8_mouse A-kinase anchor protein 8 Akap8 Akap95 A-kinase anchor protein 95 kDa protein protein mouse Mus musculus Mouse MEQGYGGYGAWSAGPANTQGTYGSGMTSWQGYENYNYYNAQNTSVPAGTPYSYGPASWEATKTNDGGLAAGSPAMHVASFAPEPCTDNSDSLIAKINQRLDMLSKEGGRGGISSGGEGVQDRDSSFRFQPYESYDARPCIPEHNPYRPGYGYDYDFDLGTDRNGSFGGTFNDCRDPAPERGSLDGFLRGRGQGRFQDRSNSSTFIRSDPFMPPSASEPLSTTWNELNYMGGRGLGGPSTSRPPPSLFSQSMAPDYSMMGMQGVGGFGGTMPYGCGRSQTRIRDWPRRRGFERFGPDNMGRKRKQFPLYEEPDAKLARADSDGDLSENDDGAGDLRSGDEEFRGEDDLCDSRKQRGEKEDEDEDVKKRREKQRRRDRMRDRAADRIQFACSVCKFRSFEDEEIQKHLQSKFHKETLRFISTKLPDKTVEFLQEYIINRNKKIEKRRQELLEKESPKPKPDPFKGIGQEHFFKKIEAAHCLACDMLIPAQHQLLQRHLHSVDHNHNRRLAAEQFKKTSLHVAKSVLNNKHIVKMLEKYLKGEDPFVNETADLETEGDENVGEEKEETPEEVAAEVLAEVITAAVKAVEGEGEPAAAHSDVLTEVEGPVDTAEASSDPHTEKLLEEQTCEAASETRSIEDKTRGEAAEARNEAAMPTADAGSTLPVIAIPGIMEDELEQTGAEAKDIPTE BC1E320216A47E10 fxr2_mouse Fragile X mental retardation syndrome-related protein 2 Fxr2 Fxr2h protein protein mouse Mus musculus Mouse MGGLASGGDVEPGLPVEVRGSNGAFYKGFVKDVHEDSVTIFFENNWQSERQIPFGDVRLPPPADYNKEITEGDEVEVYSRANEQEPCGWCLARVRMMKGDFYVIEYAACDATYNEIVTLERLRPVNSSSLATKGSFFKVTMAVPEDLREACSNENVHKEFKKALGANCIFLNITNSELFILSTTEAPVKRASLLGDMHFRSLRTKLLLMSRNEEATKHLETSKQLAAAFQEEFTVREDLMGLAIGTHGANIQQARKVPGVTAIELGEETCTFRIYGETPEACRQARSYLEFSEDSVQVPRDLVGKVTGKTGKVIQGIVDKSGVVRVRVEGDNDKKNPKEGGMVPFIFVGTRENISNAQALLEYHLSYLQEVETLRLERLQIDEQLRQIGAGFRPPGSGRGGSGGGSDKAGYTTDESSSSSLHTTRTYGGSYGGRGRGRRTGGPAYGPSSDPSTASETESEKREESNRAGPGDRDPPSRGEESRRRRLGPGKGPPPVPRPTSRYNSSSISSVLKDPDSNPYSLLDTSEPEPPVDSEPGEPPPASARRRRSRRRRTDEDRTVMDGALESDGPNMTENGLEDESRPQRRNRSRRRRNRGNRTDGSISGDRQPVTVADYISRAESQSRQRPLGRTEPSEDSLSGQKGDSVSKLPKGPSENGELSAPLELGSLVNGVS RNA-binding protein mint 01F6814D4C3B5654 kap0_human cAMP-dependent protein kinase type I-alpha regulatory subunit Tissue-specific extinguisher 1 PRKAR1A PKR1 PRKAR1 TSE1 protein protein human Homo sapiens Human MESGSTAASEEARSLRECELYVQKHNIQALLKDSIVQLCTARPERPMAFLREYFERLEKEEAKQIQNLQKAGTRTDSREDEISPPPPNPVVKGRRRRGAISAEVYTEEDAASYVRKVIPKDYKTMAALAKAIEKNVLFSHLDDNERSDIFDAMFSVSFIAGETVIQQGDEGDNFYVIDQGETDVYVNNEWATSVGEGGSFGELALIYGTPRAATVKAKTNVKLWGIDRDSYRRILMGSTLRKRKMYEEFLSKVSILESLDKWERLTVADALEPVQFEDGQKIVVQGEPGDEFFIILEGSAAVLQRRSENEEFVEVGRLGPSDYFGEIALLMNRPRAATVVARGPLKCVKLDRPRFERVLGPCSDILKRNIQQYNSFVSLSV DIP protein P10644 original sequence version: 173 DIP protein P10644 original sequence version: 127 DIP protein P10644 original sequence version: 134 DIP protein P10644 original sequence version: 179 2D04F08CE8857A6D akap8_human A-kinase anchor protein 8 AKAP8 AKAP95 A-kinase anchor protein 95 kDa protein protein human Homo sapiens Human MDQGYGGYGAWSAGPANTQGAYGTGVASWQGYENYNYYGAQNTSVTTGATYSYGPASWEAAKANDGGLAAGAPAMHMASYGPEPCTDNSDSLIAKINQRLDMMSKEGGRGGSGGGGEGIQDRESSFRFQPFESYDSRPCLPEHNPYRPSYSYDYEFDLGSDRNGSFGGQYSECRDPARERGSLDGFMRGRGQGRFQDRSNPGTFMRSDPFVPPAASSEPLSTPWNELNYVGGRGLGGPSPSRPPPSLFSQSMAPDYGVMGMQGAGGYDSTMPYGCGRSQPRMRDRDRPKRRGFDRFGPDGTGRKRKQFQLYEEPDTKLARVDSEGDFSENDDAAGDFRSGDEEFKGEDELCDSGRQRGEKEDEDEDVKKRREKQRRRDRTRDRAADRIQFACSVCKFRSFDDEEIQKHLQSKFHKETLRFISTKLPDKTVEFLQEYIVNRNKKIEKRRQELMEKETAKPKPDPFKGIGQEHFFKKIEAAHCLACDMLIPAQPQLLQRHLHSVDHNHNRRLAAEQFKKTSLHVAKSVLNNRHIVKMLEKYLKGEDPFTSETVDPEMEGDDNLGGEDKKETPEEVAADVLAEVITAAVRAVDGEGAPAPESSGEPAEDEGPTDTAEAGSDPQAEQLLEEQVPCGTAHEKGVPKARSEAAEAGNGAETMAAEAESAQTRVAPAPAAADAEVEQTDAESKDAVPTE DIP protein O43823 original sequence version: 118 CBCD5F014FD94B66 pygm_mouse Glycogen phosphorylase, muscle form Pygm Myophosphorylase protein protein mouse Mus musculus Mouse MSRPLSDQDKRKQISVRGLAGVENVSELKKNFNRHLHFTLVKDRNVATPRDYYFALAHTVRDHLVGRWIRTQQHYYEKDPKRIYYLSLEFYMGRTLQNTMVNLALENACDEATYQLGLDMEELEEIEEDAGLGNGGLGRLAACFLDSMATLGLAAYGYGIRYEFGIFNQKICGGWQMEEADDWLRYGNPWEKARPEFTLPVHFYGRVEHTSQGAKWVDTQVVLAMPYDTPVPGYRNNVVNTMRLWSAKAPNDFNLKDFNVGGYIQAVLDRNLAENISRVLYPNDNFFEGKELRLKQEYFVVAATLQDIIRRFKSSKFGSRDPVRTNFDAFPDKVAIQLNDTHPSLAIPELMRILVDLERLDWDKAWDVTVKTCAYTNHTVLPEALERWPVHLMETLLPRHLQIIYEINQRFLNRVAAAFPGDVDRLRRMSLVEEGAVKRINMAHLCIAGSHAVNGVARIHSEILKKTIFKDFYELEPHKFQNKTNGITPRRWLVLCNPGLAEVIAERIGEDYISDLDQLRKLLSYVDDEAFIRDVAKVKQENKLKFSAYLEREYKVHINPNSLFDVQVKRIHEYKRQLLNCLHIITLYNRIKREPNRFMVPRTIMIGGKAAPGYHMAKMIIKLITAIGDVVNHDPAVGDRLRVIFLENYRVSLAEKVIPAADLSEQISTAGTEASGTGNMKFMLNGALTIGTMDGANVEMAEEAGEENFFIFGMRVEDVERLDQRGYNAQEYYDRIPELRQIIEQLSSGFFSPKQPDLFKDIVNMLMHHDRFKVFADYEEYIKCQDKVSELYKNPREWTRMVIRNIATSGKFSSDRTIAQYAREIWGVEPSRQRLPAPDEKI B55CC8EFF517DCD9 ago2_mouse Protein argonaute-2 Ago2 Argonaute RISC catalytic component 2 Eif2c2 Eukaryotic translation initiation factor 2C 2 Kiaa4215 Piwi/argonaute family protein meIF2C2 Protein slicer protein protein mouse Mus musculus Mouse MYSGAGPVLASPAPTTSPIPGYAFKPPPRPDFGTTGRTIKLQANFFEMDIPKIDIYHYELDIKPEKCPRRVNREIVEHMVQHFKTQIFGDRKPVFDGRKNLYTAMPLPIGRDKVELEVTLPGEGKDRIFKVSIKWVSCVSLQALHDALSGRLPSVPFETIQALDVVMRHLPSMRYTPVGRSFFTASEGCSNPLGGGREVWFGFHQSVRPSLWKMMLNIDVSATAFYKAQPVIEFVCEVLDFKSIEEQQKPLTDSQRVKFTKEIKGLKVEITHCGQMKRKYRVCNVTRRPASHQTFPLQQESGQTVECTVAQYFKDRHKLVLRYPHLPCLQVGQEQKHTYLPLEVCNIVAGQRCIKKLTDNQTSTMIRATARSAPDRQEEISKLMRSASFNTDPYVREFGIMVKDEMTDVTGRVLQPPSILYGGRNKAIATPVQGVWDMRNKQFHTGIEIKVWAIACFAPQRQCTEVHLKSFTEQLRKISRDAGMPIQGQPCFCKYAQGADSVEPMFRHLKNTYAGLQLVVVILPGKTPVYAEVKRVGDTVLGMATQCVQMKNVQRTTPQTLSNLCLKINVKLGGVNNILLPQGRPPVFQQPVIFLGADVTHPPAGDGKKPSIAAVVGSMDAHPNRYCATVRVQQHRQEIIQDLAAMVRELLIQFYKSTRFKPTRIIFYRDGVSEGQFQQVLHHELLAIREACIKLEKDYQPGITFIVVQKRHHTRLFCTDKNERVGKSGNIPAGTTVDTKITHPTEFDFYLCSHAGIQGTSRPSHYHVLWDDNRFSSDELQILTYQLCHTYVRCTRSVSIPAPAYYAHLVAFRARYHLVDKEHDSAEGSHTSGQSNGRDHQALAKAVQVHQDTLRTMYFA DIP protein Q8CJG0 original sequence version: 61 DIP protein Q8CJG0 original sequence version: 83 A4E13C633846062C ks6a1_human Ribosomal protein S6 kinase alpha-1 RPS6KA1 RSK1 90 kDa ribosomal protein S6 kinase 1 MAPKAPK1A Ribosomal S6 kinase 1 MAP kinase-activated protein kinase 1a protein protein human Homo sapiens Human MPLAQLKEPWPLMELVPLDPENGQTSGEEAGLQPSKDEGVLKEISITHHVKAGSEKADPSHFELLKVLGQGSFGKVFLVRKVTRPDSGHLYAMKVLKKATLKVRDRVRTKMERDILADVNHPFVVKLHYAFQTEGKLYLILDFLRGGDLFTRLSKEVMFTEEDVKFYLAELALGLDHLHSLGIIYRDLKPENILLDEEGHIKLTDFGLSKEAIDHEKKAYSFCGTVEYMAPEVVNRQGHSHSADWWSYGVLMFEMLTGSLPFQGKDRKETMTLILKAKLGMPQFLSTEAQSLLRALFKRNPANRLGSGPDGAEEIKRHVFYSTIDWNKLYRREIKPPFKPAVAQPDDTFYFDTEFTSRTPKDSPGIPPSAGAHQLFRGFSFVATGLMEDDGKPRAPQAPLHSVVQQLHGKNLVFSDGYVVKETIGVGSYSECKRCVHKATNMEYAVKVIDKSKRDPSEEIEILLRYGQHPNIITLKDVYDDGKHVYLVTELMRGGELLDKILRQKFFSEREASFVLHTIGKTVEYLHSQGVVHRDLKPSNILYVDESGNPECLRICDFGFAKQLRAENGLLMTPCYTANFVAPEVLKRQGYDEGCDIWSLGILLYTMLAGYTPFANGPSDTPEEILTRIGSGKFTLSGGNWNTVSETAKDLVSKMLHVDPHQRLTAKQVLQHPWVTQKDKLPQSQLSHQDLQLVKGAMAATYSALNSSKPTPQLKPIESSILAQRRVRKLPSTTL DIP protein Q15418 original sequence version: 111 DIP protein Q15418 original sequence version: 85 765731A4442A53DF akap8-rps6ka1-1 4 16 unspecified role unspecified role prey prey 13 unspecified role unspecified role bait bait physical association physical association 6A akap8-rps6ka1-2 5 16 unspecified role unspecified role neutral component neutral component 13 tag fluorescence tag visualisation by fluorescence tag fluorescence immunostaining immunostaining unspecified role unspecified role neutral component neutral component nucl transfection nucleic acid transfection nucl transfection region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range O43823:p.[Arg290Ser;Lys304Asn;Arg305Ser] mutation mutation certain certain sequence position certain certain certain sequence position certain R S certain certain sequence position certain certain certain sequence position certain K N certain certain sequence position certain certain certain sequence position certain R S Wed May 02 19:16:05 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg290ser,lys304asn,arg305ser' Tue Jun 30 18:48:29 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Arg290Ser;Lys304Asn;Arg305Ser]' colocalization colocalization 7c rps6ka1-akap8-1 2 16 unspecified role unspecified role bait bait 13 unspecified role unspecified role prey prey nucl transfection nucleic acid transfection nucl transfection n-terminus gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range O43823:p.[Arg290Ser;Lys304Asn;Arg305Ser] mutation mutation certain certain sequence position certain certain certain sequence position certain R S certain certain sequence position certain certain certain sequence position certain K N certain certain sequence position certain certain certain sequence position certain R S Wed May 02 16:07:26 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg290ser,lys304asn,arg305ser' Tue Jun 30 16:12:18 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Arg290Ser;Lys304Asn;Arg305Ser]' physical association physical association supplemental 3E rps6ka1-akap8-2 2 16 unspecified role unspecified role bait bait 13 unspecified role unspecified role prey prey nucl transfection nucleic acid transfection nucl transfection region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association supplemental 3E rps6ka1-akap8-3 2 16 unspecified role unspecified role bait bait 13 unspecified role unspecified role prey prey nucl transfection nucleic acid transfection nucl transfection n-terminus gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range O43823:p.[Lys304Ser;Arg305Asn] mutation mutation certain certain sequence position certain certain certain sequence position certain K S certain certain sequence position certain certain certain sequence position certain R N Fri Jul 22 11:03:59 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'lys204asn,arg305ser' Wed May 02 18:02:21 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys304ser,arg305asn' Tue Jun 30 16:09:49 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Lys304Ser;Arg305Asn]' physical association physical association supplemental 3E prkar1a-rps6ka1-1 1 12 unspecified role unspecified role prey prey human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 7 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 9 unspecified role unspecified role bait bait physical association physical association 2D N-palmitoylated pseudosubstrate (PS) region of PRKAR1A RPS6KA1/RSK1 mutant ser221ala also interacts and association is impaired by PS peptide rps6ka1-actb-1 3 15 unspecified role unspecified role prey prey 11 unspecified role unspecified role prey prey 10 unspecified role unspecified role prey prey 6 unspecified role unspecified role prey prey 14 unspecified role unspecified role prey prey 8 unspecified role unspecified role bait bait association association supplemental S1A and B Keratin 73 found associated; judged as contaminant