HPIDb Host Pathogen Interaction database http://www.agbase.msstate.edu/hpi/main.html Classical swine fever virus N(pro) limits type I interferon induction in plasmacytoid dendritic cells by interacting with interferon regulatory factor 7. Classical swine fever virus N(pro) limits type I interferon induction in plasmacytoid dendritic cells by interacting with interferon regulatory factor 7. Journal of virology 2011 imex curation Fiebach AR., Guzylack-Piriou L., Python S., Summerfield A., Ruggli N. Virus - Publications including interactions involving viral proteins nicolas.ruggli@ivi.admin.ch 17-Oct-2016: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. gal4 vp16 complement gal4 vp16 complementation karyoplasmic interaction ion strategy KISS mammalian two hybrid gal4 vp16 complement predetermined predetermined participant predetermined Fiebach AR., Guzylack-Piriou L., Python S., Summerfield A., Ruggli N. Virus - Publications including interactions involving viral proteins nicolas.ruggli@ivi.admin.ch Accepted 2016-SEP-29 AT 12:29 BST AT 12:29 BST by MGT Journal of virology 2011 imex curation Classical swine fever virus N(pro) limits type I interferon induction in plasmacytoid dendritic cells by interacting with interferon regulatory factor 7. Classical swine fever virus N(pro) limits type I interferon induction in plasmacytoid dendritic cells by interacting with interferon regulatory factor 7. Journal of virology 2011 imex curation Fiebach AR., Guzylack-Piriou L., Python S., Summerfield A., Ruggli N. Virus - Publications including interactions involving viral proteins nicolas.ruggli@ivi.admin.ch 17-Oct-2016: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Fiebach AR., Guzylack-Piriou L., Python S., Summerfield A., Ruggli N. Virus - Publications including interactions involving viral proteins nicolas.ruggli@ivi.admin.ch Accepted 2016-SEP-29 AT 12:29 BST AT 12:29 BST by MGT Journal of virology 2011 imex curation a0zvr0_pig Irf7 IRF7 protein protein pig Sus scrofa (Pig) Pig MAAAPDRGCPRVLFGDWLLGEVSSGRYAGLRWLDEARTRFRVPWKHFARKDLGESDSRIFKAWAVARGRWPPRSSEGAQPIPEGALRASWKTNFRCALHSTRRFVMLQDNSGDPTDPHKVYMINPELGCGEGPGFNPGEEEALEDAPPPRGELPGPRLAAHTGERLRHELARPSPEPCTPSPAGDGGDLLLQALQQSCMEDHLLEAAWGVDLTPPEVPYAGPLPAPVPWKPQEAEPCTTLYPRVCTRMAGERGAASPSCSQLGLHAGPSLEALDLTIMYKGRTVLQEMVGGPSCVLLYGSPGPAGAAAEPQQVAFPSPAELPDQKQLHYTEKLLQHVAPGLQLEFRGPKLWARRLGKCKVYWEVGGPLGSGSPSTPACLLPRDCDTPIFDFGTFFQELMEFRARRRQGSPHYTIYLGFGQDLSVGRPKEKSLVLVKLEPWLCRTQLESVQREGVSSLDSGSLSLCLSPSNSLYDELERFLMEVEQPA C8A8EA11D86FDA37 irf3_pig Interferon regulatory factor 3 IRF3 protein protein pig Sus scrofa (Pig) Pig MGTQKPRILPWLISQLNQGQLEGVAWLDEGHTRFRIPWKHGLRQDAQQEDFGIFQAWAEASGAYTPGKDKPDLPTWKRNFRSALNRKEALRLAEDHSKDPHDPHKIYEFVTSGVGDFPEPDTSLDLSGRYSTSDTHEDSLDKLLSGMDLASDAGPQSLTLALEQPPQLSLSPSVDAPASCPNLGVRENPLKQLLANDDEWEFQVTVFYRGCQVFQQTVCSPGGLRLVGSEAEDGTLAGQPVRLPDPAASLTDRGVADYVRRVLSCLGGGLALWRAGQWLWAQRLGHCHVYWAMGEELIPDSGHKPDGEVPKDREGGVFDLGPFIEDLIAFIEGSRRSPRYTLWFCMGQSWPQDEPWVKRLVMVKVVPMCLRALVDMARDGGASSLENTVDLHISNSHPLSLTSDQYKACLRDLVEDMDF 8B731861BE002284 q68871_9flav Autoprotease p20 Capsid protein C Cysteine protease NS2 E(rns) glycoprotein Envelope glycoprotein E1 Envelope glycoprotein E2 Genome polyprotein N-terminal protease NS5B Non-structural protein 2 Non-structural protein 2-3 Non-structural protein 3 Non-structural protein 4A Non-structural protein 4B Non-structural protein 5A RNA-directed RNA polymerase Serine protease NS3 gp33 gp44/48 gp55 Viroporin p7 protein protein classical swine fever virus - alfort/187 classical swine fever virus - alfort/187 MELNHFELLYKTNKQKPMGVEEPVYDATGKPLFGDPSEVHPQSTLKLPHDRGRGNIKTTLKNLPRKGDCRSGNHLGPVSGIYVKPGPVFYQDYMGPVYHRAPLEFFSEAQFCEVTKRIGRVTGSDGRLYHIYVCIDGCILLKLAKRGEPRTLKWIRNFTDCPLWVTSCSDDGASGSKEKKPDRINKGKLKIAPKEHEKDSRTKPPDATIVVEGVKYQVKKKGKVKGKNTQDGLYHNKNKPPESRKKLEKALLAWAVIAIMLYQPVEAENITQWNLSDNGTNGIQHAMYLRGISRSLHGIWPEKICKGVPTYLATDTELKEIQGMMDASEGTNYTCCKLQRHEWNKHGWCNWYNIDPWIQLMNRTQANLAEGPPAKECAVTCRYDKDADVNVVTQARNRPTTLTGCKKGKNFSFAGTVIEGPCNFNVSVEDILYGDHECGSLLQDTALYLVDGMTNTIENARQGAARVTSWLGRQLRIAGKRLEGRSKTWFGAYALSPYCNVTSKIGYIWYTNNCTPACLPKNTKIIGPGKFDTNAEDGKILHEMGGHLSEFLLLSLVVLSDFAPETASALYLILHYVIPQSHEEPEGCDTNQLNLTVELRTEDVIPSSVWNVGKYVCVRPDWWPYETKVALLFEEAGQVVKLALRALRDLTRVWNSASTTAFLICLIKVLRGQIVQGVIWLLLVTGAQGQLACKEDYRYAISSTNEIGLLGAGGLTTTWKEYNHDLQLNDGTVKAICVAGSFKVTALNVVSRRYLASLHKEALPTSVTFELLFDGTNPSTEEMGDDFGFGLCPFDTSPVVKGKYNTTLLNGSAFYLVCPIGWTGVIECTAVSPTTLRTEVVKTFRRDKPFPHRMDCVTTTVENEDLFYCKLGGNWTCVKGEPVVYTGGLVKQCRWCGFDFNEPDGLPHYPIGKCILANETGYRIVDSTDCNRDGVVISTEGSHECLIGNTTVKVHASDERLGPMPCRPKEIVSSAGPVRKTSCTFNYAKTLKNKYYEPRDSYFQQYMLKGEYQYWFDLDVTDRHSDYFAEFVVLVVVALLGGRYILWLIVTYIVLTEQLAAGLPLGQGEVVLIGNLITHTDIEVVVYFLLLYLVMRDEPIKKWILLLFHAMTNNPVKTITVALLMVSGVARGGKIDGGWQRLPETSFDIQLALTVIVVAVMLLAKRDPTTVPLVVTVATLRTAKMTNGLSTDIAIATVSTALLTWTYISDYYRYKTWLQYLISTVTGIFLIRVLKGIGELDLHTPTLPSYRPLFFILVYLISTAVVTRWNLDIAGLLLQCVPTLLMVFTMWADILTLILILPTYELTKLYYLKEVKIGAERGWLWKTNFKRVNDIYEVDQAGEGVYLFPSKQKTSSITGTMLPLIKAILISCISNKWQFIYLLYLIFEVSYYLHKKIIDEIAGGTNFISRLVAALIEANWAFDNEEVRGLKKFFLLSSRVKELIIKHKVRNEVMVHWFGDEEVYGMPKLVGLVKAATLSKNKHCILCTVCEDREWRGETCPKCGRFGPPMTCGMTLADFEEKHYKRIFFREDQSEGPVREEYAGYLQYRARGQLFLRNLPVLATKVKMLLVGNLGTEVGDLEHLGWVLRGPAVCKKVTEHEKCTTSIMDKLTAFFGVMPRGTTPRAPVRFPTSLLKIRRGLETGWAYTHQGGISSVDHVTCGKDLLVCDTMGRTRVVCQSNNKMTDESEYGVKTDSGCPEGARCYVFNPEAVNISGTKGAMVHLQKTGGEFTCVTASGTPAFFDLKNLKGWSGLPIFEASSGRVVGRVKVGKNEDSKPTKLMSGIQTVSKSTTDLTEMVKKITTMNRGEFRQITLATGAGKTTELPRSVIEEIGRHKRVLVLIPLRAAAESVYQYMRQKHPSIAFNLRIGEMKEGDMATGITYASYGYFCQMPQPKLRAAMVEYSFIFLDEYHCATPEQLAIMGKIHRFSENLRVVAMTATPAGTVTTTGQKHPIEEFIAPEVMKGEDLGSEYLDIAGLKIPVEEMKSNMLVFVPTRNMAVETAKKLKAKGYNSGYYYSGEDPSNLRVVTSQSPYVVVATNAIESGVTLPDLDVVVDTGLKCEKRIRLSPKMPFIVTGLKRMAVTIGEQAQRRGRVGRVKPGRYYRSQETPVGSKDYHYDLLQAQRYGIEDGINITKSFREMNYDWSLYEEDSLMITQLEILNNLLISEELPMAVKNIMARTDHPEPIQLAYNSYETQVPVLFPKIKNGEVTDSYDNYTFLNARKLGDDVPPYVYATEDEDLAVELLGLDWPDPGNQGTVEAGRALKQVVGLSTAENALLVALFGYVGYQALSKRHIPVVTDIYSIEDHRLEDTTHLQYAPNAIKTEGKETELKELAQGDVQRCVEAMTNYAREGIQFMKSQALKVKETPTYKETMDTVTDYVKKFMEALTDSKEDIIKYGLWGTHTALYKSICARLGSETAFATLVVKWLAFGGESIADHVKQAATDLVVYYIINRPQFPGDTETQQEGRKFVASLLVSALVTYTYKSWNYNNLSKIVEPALATLPYAATALKLFAPTRLESVVILSTAIYKTYLSIRRGKSDGLLGTGVSAAMEIMSQNPVSVGIAVMLGVGAVAAHNAIEASEQKRTLLMKVFVKNFLDQAATDELVKESPEKIIMALFEAVQTVGNPLRLVYHLYGVFYKGWEAKELAQRTAGRNLFTLIMFEAVELLGVDSEGKIRQLSSNYILELLYKFRDSIKSSVREMAISWAPAPFSCDWTPTDDRIGLPQDNFLQVETKCPCGYKMKAVKNCAGELRLLEEEGSFLCRNKFGRGSRNYRVTKYYDDNLSEIKPVIRMEGHVELYYKGATIKLDFNNSKTILATDKWEVDHSTLVRVLKRHTGAGYHGAYLGEKPNHKHLIERDCATITKDKVCFLKMKRGCAFTYDLSLHNLTRLIELVHKNNLEDKEIPAVTVTTWLAYTFVNEDIGTIKPAFGEKVTPEMQEEITLQPAVVVDTTDVTVTVVGEAPTMTTGETPTAFTSSGSDPKGQQVLKLGVGEGQYPGTNPQRASLHEAIQGADERPSVLILGSDKATSNRVKTAKNVKVYRGRDPLEVRDMMRRGKILVIALSRVDNALLKFVDYKGTFLTRETLEALSLGRPKKKNITKAEAQWLLCLEDQMEELPDWFAAGEPIFLEANIKHDRYHLVGDIATIKEKAKQLGATDSTKISKEVGAKVYSMKLSNWVMQEENKQGNLTPLFEELLQQCPPGGQNKTAHMVSAYQLAQGNWMPTSCHVFMGTISARRTKTHPYEAYVKLRELVEEHKMKTLCPGSSLGKHNEWIIGKIKYQGNLRTKHMLNPGKVAEQLCREGHRRNVYNKTIGSVMTATGIRLEKLPVVRAQTDTTNFHQAIRDKIDKEENLQTPGLHKKLMEVFNALKRPELESSYDAVEWEELERGINRKGAAGFFERKNIGEILDSEKNKVEEIIDNLKKGRNIKYYETAIPKNEKRDVNDDWTSGDFVDEKKPRVIQYPEAKTRLAITKVMYKWVKQKPVVIPGYEGKTPLFQIFDKVKKEWDQFQNPVAVSFDTKAWDTQVTTKDLELIKDIQKYYFKKKWHKFIDTLTMHMSEVPVISADGEVYIRKGQRGSGQPDTSAGNSMLNVLTMVYAFCEATGVPYKSFDRVAKIHVCGDDGFLITERALGEKFASKGVQILYEAGKPQKITEGDKMKVAYQFDDIEFCSHTPIQVRWSDNTSSYMPGRNTTTILAKMATRLDSSGERGTIAYEKAVAFSFLLMYSWNPLIRRICLLVLSTELQVKPGKSTTYYYEGDPISAYKEVIGHNLFDLKRTSFEKLAKLNLSMSVLGAWTRHTSKRLLQDCVNMGVKEGNWLVNADRLVSSKTGNRYIPGEGHTLQGRHYEELVLARKQINNFQGTDRYNLGPIVNMVLRRLRVMMMTLIGRGV npro-irf7-1 1 3 unspecified role unspecified role prey prey 5 unspecified role unspecified role bait bait Q68871:p.Cys112Arg mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain C R Wed May 02 15:03:54 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'cys112arg' Tue Jun 30 15:55:38 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Cys112Arg' Q68871:p.Cys69Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain C A Mon Oct 03 15:02:33 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'cys69ala' Wed May 02 19:02:22 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'cys69ala' Tue Jun 30 17:50:18 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Cys69Ala' Q68871:p.Cys112Ala mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain C A Wed May 02 20:29:13 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'cys112ala' Tue Jun 30 16:07:06 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Cys112Ala' Q68871:p.Cys134Ala mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain C A Wed May 02 16:04:27 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'cys134ala' Tue Jun 30 16:36:46 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Cys134Ala' Q68871:p.Cys138Ala mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain C A Wed May 02 15:05:51 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'cys138ala' Tue Jun 30 19:00:08 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Cys138Ala' Q68871:p.Cys161Ala mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain C A Wed May 02 14:56:52 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'cys161ala' Tue Jun 30 16:45:19 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Cys161Ala' Q68871:p.Asp136Asn mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain D N Wed May 02 18:30:56 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp136asn' Tue Jun 30 18:41:56 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp136Asn' Npro region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association 1A, 2 and 3F npro-irf7-2 1 5 unspecified role unspecified role bait bait Npro region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 4 inhibitor inhibitor unspecified role unspecified role n terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range 3 unspecified role unspecified role prey prey physical association physical association 1A npro-irf7-4 2 5 unspecified role unspecified role prey prey Npro region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 3 unspecified role unspecified role bait bait n terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association 1B irf7-npro-1 2 5 unspecified role unspecified role bait bait n terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range Npro region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 3 unspecified role unspecified role prey prey physical association physical association 1B npro-irf7-3 1 5 unspecified role unspecified role bait bait Npro region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 3 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association 3F npro-irf3-4 1 5 unspecified role unspecified role bait bait Q68871:p.Cys112Arg mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain C R Wed May 02 20:27:30 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'cys112arg' Tue Jun 30 18:43:59 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Cys112Arg' Npro region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 4 unspecified role unspecified role prey prey physical association physical association 1,2 and 3E