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  <entry>
    <source releaseDate="2022-02-04">
      <names>
        <shortLabel>IntAct</shortLabel>
        <fullName>European Bioinformatics Institute</fullName>
        <alias type="synonym" typeAc="MI:1041">IntAct</alias>
        <alias type="synonym" typeAc="MI:1041">intact</alias>
      </names>
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      </xref>
      <attributeList>
        <attribute name="url" nameAc="MI:0614">http://www.ebi.ac.uk/</attribute>
        <attribute name="search-url" nameAc="MI:0615">http://www.ebi.ac.uk/intact/query/${ac}</attribute>
        <attribute name="id-validation-regexp" nameAc="MI:0628">EBI-[0-9]+|IA:[0-9]+</attribute>
        <attribute name="definition">INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions.</attribute>
        <attribute name="url" nameAc="MI:0614">http://www.ebi.ac.uk/intact</attribute>
        <attribute name="postaladdress">European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom</attribute>
        <attribute name="url" nameAc="MI:0614">http://www.ebi.ac.uk/intact/</attribute>
      </attributeList>
    </source>
    <experimentList>
      <experimentDescription id="1">
        <names>
          <fullName>Structure and functional implications of a complex containing a segment of U6 RNA bound by a domain of Prp24.</fullName>
        </names>
        <bibref>
          <xref>
            <primaryRef db="pubmed" dbAc="MI:0446" id="20181740" refType="primary-reference" refTypeAc="MI:0358"/>
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          </xref>
          <attributeList>
            <attribute name="publication title" nameAc="MI:1091">Structure and functional implications of a complex containing a segment of U6 RNA bound by a domain of Prp24.</attribute>
            <attribute name="journal" nameAc="MI:0885">RNA (New York, N.Y.)</attribute>
            <attribute name="publication year" nameAc="MI:0886">2010</attribute>
            <attribute name="curation depth" nameAc="MI:0955">imex curation</attribute>
            <attribute name="imex curation" nameAc="MI:0959"/>
            <attribute name="author-list" nameAc="MI:0636">Martin-Tumasz S., Reiter NJ., Brow DA., Butcher SE.</attribute>
            <attribute name="contact-email" nameAc="MI:0634">butcher@biochem.wisc.edu</attribute>
            <attribute name="author-announcement">20-Dec-2016: Contacted by IntAct-Help.</attribute>
            <attribute name="full coverage" nameAc="MI:0957">Only protein-protein interactions</attribute>
            <attribute name="imex curation" nameAc="MI:0959">imex curation</attribute>
          </attributeList>
        </bibref>
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        </xref>
        <hostOrganismList>
          <hostOrganism ncbiTaxId="-1">
            <names>
              <shortLabel>in vitro</shortLabel>
              <fullName>In vitro</fullName>
            </names>
          </hostOrganism>
        </hostOrganismList>
        <interactionDetectionMethod>
          <names>
            <shortLabel>nmr</shortLabel>
            <fullName>nuclear magnetic resonance</fullName>
            <alias type="go synonym" typeAc="MI:0303">NMR</alias>
            <alias type="synonym" typeAc="MI:1041">NMR</alias>
            <alias type="synonym" typeAc="MI:1041">nmr</alias>
          </names>
          <xref>
            <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0077" refType="identity" refTypeAc="MI:0356"/>
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          </xref>
        </interactionDetectionMethod>
        <participantIdentificationMethod>
          <names>
            <shortLabel>predetermined</shortLabel>
            <fullName>predetermined participant</fullName>
            <alias type="synonym" typeAc="MI:1041">predetermined</alias>
          </names>
          <xref>
            <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0396" refType="identity" refTypeAc="MI:0356"/>
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          </xref>
        </participantIdentificationMethod>
        <attributeList>
          <attribute name="author-list" nameAc="MI:0636">Martin-Tumasz S., Reiter NJ., Brow DA., Butcher SE.</attribute>
          <attribute name="contact-email" nameAc="MI:0634">butcher@biochem.wisc.edu</attribute>
          <attribute name="accepted">Accepted 2016-OCT-31 AT 10:23 GMT AT 10:23 GMT by ORCHARD</attribute>
          <attribute name="journal" nameAc="MI:0885">RNA (New York, N.Y.)</attribute>
          <attribute name="publication year" nameAc="MI:0886">2010</attribute>
          <attribute name="curation depth" nameAc="MI:0955">imex curation</attribute>
        </attributeList>
      </experimentDescription>
    </experimentList>
    <interactorList>
      <interactor id="2">
        <names>
          <shortLabel>snru6_yeast</shortLabel>
          <fullName>small nuclear RNA U6 Yeast</fullName>
        </names>
        <xref>
          <primaryRef db="rnacentral" dbAc="MI:1357" id="URS00006184A9_559292" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="intact" dbAc="MI:0469" id="EBI-10824938" refType="identity" refTypeAc="MI:0356"/>
        </xref>
        <interactorType>
          <names>
            <shortLabel>snrna</shortLabel>
            <fullName>small nuclear rna</fullName>
            <alias type="go synonym" typeAc="MI:0303">snRNA</alias>
            <alias type="synonym" typeAc="MI:1041">snrna</alias>
          </names>
          <xref>
            <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0607" refType="identity" refTypeAc="MI:0356"/>
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          </xref>
        </interactorType>
        <organism ncbiTaxId="559292">
          <names>
            <shortLabel>yeast</shortLabel>
            <fullName>Saccharomyces cerevisiae</fullName>
            <alias type="synonym" typeAc="MI:1041">Baker's yeast</alias>
          </names>
        </organism>
        <sequence>GUUCGCGAAGUAACCCUUCGUGGACAUUUGGUCAAUUUGAAACAAUACAGAGAUGAUCAGCAGUUCCCCUGCAUAAGGAUGAACCGUUUUACAAAGAGAUUUAUUUCGUUUU</sequence>
      </interactor>
      <interactor id="3">
        <names>
          <shortLabel>prp24_yeast</shortLabel>
          <fullName>U4/U6 snRNA-associated-splicing factor PRP24</fullName>
          <alias type="gene name" typeAc="MI:0301">PRP24</alias>
          <alias type="orf name" typeAc="MI:0306">YM8156.10C</alias>
          <alias type="locus name" typeAc="MI:0305">YMR268C</alias>
        </names>
        <xref>
          <primaryRef db="uniprotkb" dbAc="MI:0486" id="P49960" version="SP_57" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="uniprotkb" dbAc="MI:0486" id="D6W094" version="SP_88" refType="secondary-ac" refTypeAc="MI:0360"/>
          <secondaryRef db="uniprotkb" dbAc="MI:0486" id="E9P966" version="SP_92" refType="secondary-ac" refTypeAc="MI:0360"/>
          <secondaryRef db="ensemblfungi" id="YMR268C" version="SP_164" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="intact" dbAc="MI:0469" id="EBI-212" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="refseq" dbAc="MI:0481" id="NP_013995.1"/>
          <secondaryRef db="sgd" dbAc="MI:0484" id="S000004881"/>
          <secondaryRef db="mint" dbAc="MI:0471" id="P49960"/>
          <secondaryRef db="dip" dbAc="MI:0465" id="DIP-2570N"/>
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          <secondaryRef db="go" dbAc="MI:0448" id="GO:0003723" version="SP_164"/>
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          <secondaryRef db="go" dbAc="MI:0448" id="GO:0017069" version="SP_164"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0017070" version="SP_164"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:1990904" version="SP_164"/>
          <secondaryRef db="interpro" dbAc="MI:0449" id="IPR000504" version="SP_164"/>
          <secondaryRef db="interpro" dbAc="MI:0449" id="IPR008669" version="SP_164"/>
          <secondaryRef db="interpro" dbAc="MI:0449" id="IPR012677" version="SP_164"/>
          <secondaryRef db="interpro" dbAc="MI:0449" id="IPR031766" version="SP_164"/>
          <secondaryRef db="interpro" dbAc="MI:0449" id="IPR034397" version="SP_164"/>
          <secondaryRef db="interpro" dbAc="MI:0449" id="IPR034398" version="SP_164"/>
          <secondaryRef db="interpro" dbAc="MI:0449" id="IPR035979" version="SP_164"/>
          <secondaryRef db="rcsb pdb" dbAc="MI:0460" id="2GHP" version="SP_164"/>
          <secondaryRef db="rcsb pdb" dbAc="MI:0460" id="2GO9" version="SP_164"/>
          <secondaryRef db="rcsb pdb" dbAc="MI:0460" id="2KH9" version="SP_164"/>
          <secondaryRef db="rcsb pdb" dbAc="MI:0460" id="2L9W" version="SP_164"/>
          <secondaryRef db="rcsb pdb" dbAc="MI:0460" id="4N0T" version="SP_164"/>
          <secondaryRef db="rcsb pdb" dbAc="MI:0460" id="5TF6" version="SP_164"/>
          <secondaryRef db="rcsb pdb" dbAc="MI:0460" id="5VSU" version="SP_164"/>
          <secondaryRef db="rcsb pdb" dbAc="MI:0460" id="6ASO" version="SP_164"/>
        </xref>
        <interactorType>
          <names>
            <shortLabel>protein</shortLabel>
            <fullName>protein</fullName>
          </names>
          <xref>
            <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0326" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="intact" dbAc="MI:0469" id="EBI-619654" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
            <secondaryRef db="so" dbAc="MI:0601" id="SO:0000358" refType="see-also" refTypeAc="MI:0361"/>
          </xref>
        </interactorType>
        <organism ncbiTaxId="559292">
          <names>
            <shortLabel>yeast</shortLabel>
            <fullName>Saccharomyces cerevisiae</fullName>
            <alias type="synonym" typeAc="MI:1041">Baker's yeast</alias>
          </names>
        </organism>
        <sequence>MEYGHHARPDSKRPLDEGSPAAAGLTSKKANEALTRNRELTTVLVKNLPKSYNQNKVYKYFKHCGPIIHVDVADSLKKNFRFARIEFARYDGALAAITKTHKVVGQNEIIVSHLTECTLWMTNFPPSYTQRNIRDLLQDINVVALSIRLPSLRFNTSRRFAYIDVTSKEDARYCVEKLNGLKIEGYTLVTKVSNPLEKSKRTDSATLEGREIMIRNLSTELLDENLLRESFEGFGSIEKINIPAGQKEHSFNNCCAFMVFENKDSAERALQMNRSLLGNREISVSLADKKPFLERNEVKRLLASRNSKELETLICLFPLSDKVSPSLICQFLQEEIHINEKDIRKILLVSDFNGAIIIFRDSKFAAKMLMILNGSQFQGKVIRSGTINDMKRYYNNQQNHSMKHVKPSCINMMEKGPNLQVKKKIPDKQEQMSNDDFRKMFLGE</sequence>
        <attributeList>
          <attribute name="crc64">6BCCE7A9AAC51CE0</attribute>
          <attribute name="crc64">6BCCE7A9AAC51CE0</attribute>
        </attributeList>
      </interactor>
    </interactorList>
    <interactionList>
      <interaction id="4" imexId="IM-26605-1">
        <names>
          <shortLabel>prp24 u6-1</shortLabel>
        </names>
        <xref>
          <primaryRef db="intact" dbAc="MI:0469" id="EBI-12560791" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="imex" dbAc="MI:0670" id="IM-26605-1" refType="imex-primary" refTypeAc="MI:0662"/>
        </xref>
        <experimentList>
          <experimentRef>1</experimentRef>
        </experimentList>
        <participantList>
          <participant id="5">
            <interactorRef>2</interactorRef>
            <biologicalRole>
              <names>
                <shortLabel>unspecified role</shortLabel>
                <fullName>unspecified role</fullName>
              </names>
              <xref>
                <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0499" refType="identity" refTypeAc="MI:0356"/>
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              </xref>
            </biologicalRole>
            <experimentalRoleList>
              <experimentalRole>
                <names>
                  <shortLabel>neutral component</shortLabel>
                  <fullName>neutral component</fullName>
                </names>
                <xref>
                  <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0497" refType="identity" refTypeAc="MI:0356"/>
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                </xref>
              </experimentalRole>
            </experimentalRoleList>
            <featureList>
              <feature id="6">
                <names>
                  <shortLabel>region</shortLabel>
                </names>
                <xref>
                  <primaryRef db="intact" dbAc="MI:0469" id="EBI-12560915" refType="identity" refTypeAc="MI:0356"/>
                </xref>
                <featureType>
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                    <shortLabel>sufficient to bind</shortLabel>
                    <fullName>sufficient binding region</fullName>
                    <alias type="synonym" typeAc="MI:1041">sufficient to bind</alias>
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                </featureType>
                <featureRangeList>
                  <featureRange>
                    <startStatus>
                      <names>
                        <shortLabel>certain</shortLabel>
                        <fullName>certain sequence position</fullName>
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                    </startStatus>
                    <begin position="49"/>
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                      <names>
                        <shortLabel>certain</shortLabel>
                        <fullName>certain sequence position</fullName>
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                    </endStatus>
                    <end position="54"/>
                  </featureRange>
                </featureRangeList>
              </feature>
            </featureList>
            <hostOrganismList>
              <hostOrganism ncbiTaxId="-1">
                <names>
                  <shortLabel>in vitro</shortLabel>
                  <fullName>In vitro</fullName>
                </names>
              </hostOrganism>
            </hostOrganismList>
          </participant>
          <participant id="7">
            <interactorRef>3</interactorRef>
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              <names>
                <shortLabel>unspecified role</shortLabel>
                <fullName>unspecified role</fullName>
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                <names>
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                  <fullName>neutral component</fullName>
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            <featureList>
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                  <shortLabel>region</shortLabel>
                </names>
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                </xref>
                <featureType>
                  <names>
                    <shortLabel>sufficient to bind</shortLabel>
                    <fullName>sufficient binding region</fullName>
                    <alias type="synonym" typeAc="MI:1041">sufficient to bind</alias>
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                  <xref>
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                    <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                  </xref>
                </featureType>
                <featureDetectionMethod>
                  <names>
                    <shortLabel>deletion analysis</shortLabel>
                    <fullName>deletion analysis</fullName>
                  </names>
                  <xref>
                    <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0033" refType="identity" refTypeAc="MI:0356"/>
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                </featureDetectionMethod>
                <featureRangeList>
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                        <shortLabel>certain</shortLabel>
                        <fullName>certain sequence position</fullName>
                        <alias type="synonym" typeAc="MI:1041">certain</alias>
                      </names>
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                    </startStatus>
                    <begin position="115"/>
                    <endStatus>
                      <names>
                        <shortLabel>certain</shortLabel>
                        <fullName>certain sequence position</fullName>
                        <alias type="synonym" typeAc="MI:1041">certain</alias>
                      </names>
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                      </xref>
                    </endStatus>
                    <end position="197"/>
                  </featureRange>
                </featureRangeList>
              </feature>
            </featureList>
            <hostOrganismList>
              <hostOrganism ncbiTaxId="83333">
                <names>
                  <shortLabel>ecoli</shortLabel>
                  <fullName>Escherichia coli (strain K12)</fullName>
                </names>
              </hostOrganism>
            </hostOrganismList>
          </participant>
        </participantList>
        <interactionType>
          <names>
            <shortLabel>direct interaction</shortLabel>
            <fullName>direct interaction</fullName>
          </names>
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            <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0407" refType="identity" refTypeAc="MI:0356"/>
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            <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
          </xref>
        </interactionType>
        <parameterList>
          <parameter term="kd" termAc="MI:0646" unit="molar" unitAc="MI:0648" base="10" exponent="-6" factor="90" uncertainty="10"/>
        </parameterList>
        <attributeList>
          <attribute name="figure legend" nameAc="MI:0599">Fig3, Fig5</attribute>
        </attributeList>
      </interaction>
    </interactionList>
  </entry>
</entrySet>