IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ TSG-6 regulates bone remodeling through inhibition of osteoblastogenesis and osteoclast activation. TSG-6 regulates bone remodeling through inhibition of osteoblastogenesis and osteoclast activation. The Journal of biological chemistry 2008 imex curation Mahoney DJ., Mikecz K., Ali T., Mabilleau G., Benayahu D., Plaas A., Milner CM., Day AJ., Sabokbar A., Mahoney DJ., Mikecz K., Ali T., Mabilleau G., Benayahu D., Plaas A., Milner CM., Day AJ., Sabokbar A. Dark space = 30? The authors have stated that sRANKL and all BMP proteins used here are of Human origin. caroline.milner@manchester.ac.uk,anthony.day@manchester.ac.uk 15-Nov-2017: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Mahoney DJ., Mikecz K., Ali T., Mabilleau G., Benayahu D., Plaas A., Milner CM., Day AJ., Sabokbar A., Mahoney DJ., Mikecz K., Ali T., Mabilleau G., Benayahu D., Plaas A., Milner CM., Day AJ., Sabokbar A. Kds added on the features for Link and Cub_c regions. Dark space = 30? The authors have stated that sRANKL and all BMP proteins used here are of Human origin. caroline.milner@manchester.ac.uk,anthony.day@manchester.ac.uk Accepted 2017-SEP-08 AT 12:33 BST AT 12:33 BST by PPORRAS The Journal of biological chemistry 2008 Only protein-protein interactions imex curation imex curation I have added "purified" to participants - not sure what else you might have meant.; I have added Fig 8 where appropriate TSG-6 regulates bone remodeling through inhibition of osteoblastogenesis and osteoclast activation. TSG-6 regulates bone remodeling through inhibition of osteoblastogenesis and osteoclast activation. The Journal of biological chemistry 2008 imex curation Mahoney DJ., Mikecz K., Ali T., Mabilleau G., Benayahu D., Plaas A., Milner CM., Day AJ., Sabokbar A., Mahoney DJ., Mikecz K., Ali T., Mabilleau G., Benayahu D., Plaas A., Milner CM., Day AJ., Sabokbar A. Dark space = 30? The authors have stated that sRANKL and all BMP proteins used here are of Human origin. caroline.milner@manchester.ac.uk,anthony.day@manchester.ac.uk 15-Nov-2017: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro elisa enzyme linked immunosorbent assay ELISA ELISA elisa antibody detection identification by antibody antibody detection Mahoney DJ., Mikecz K., Ali T., Mabilleau G., Benayahu D., Plaas A., Milner CM., Day AJ., Sabokbar A., Mahoney DJ., Mikecz K., Ali T., Mabilleau G., Benayahu D., Plaas A., Milner CM., Day AJ., Sabokbar A. Dark space = 30? The authors have stated that sRANKL and all BMP proteins used here are of Human origin. caroline.milner@manchester.ac.uk,anthony.day@manchester.ac.uk Accepted 2017-SEP-08 AT 12:33 BST AT 12:33 BST by PPORRAS The Journal of biological chemistry 2008 Only protein-protein interactions imex curation imex curation I have added "purified" to participants - not sure what else you might have meant.; I have added Fig 8 where appropriate bmp6_human Bone morphogenetic protein 6 BMP6 VGR VG-1-related protein protein protein human Homo sapiens Human MPGLGRRAQWLCWWWGLLCSCCGPPPLRPPLPAAAAAAAGGQLLGDGGSPGRTEQPPPSPQSSSGFLYRRLKTQEKREMQKEILSVLGLPHRPRPLHGLQQPQPPALRQQEEQQQQQQLPRGEPPPGRLKSAPLFMLDLYNALSADNDEDGASEGERQQSWPHEAASSSQRRQPPPGAAHPLNRKSLLAPGSGSGGASPLTSAQDSAFLNDADMVMSFVNLVEYDKEFSPRQRHHKEFKFNLSQIPEGEVVTAAEFRIYKDCVMGSFKNQTFLISIYQVLQEHQHRDSDLFLLDTRVVWASEEGWLEFDITATSNLWVVTPQHNMGLQLSVVTRDGVHVHPRAAGLVGRDGPYDKQPFMVAFFKVSEVHVRTTRSASSRRRQQSRNRSTQSQDVARVSSASDYNSSELKTACRKHELYVSFQDLGWQDWIIAPKGYAANYCDGECSFPLNAHMNATNHAIVQTLVHLMNPEYVPKPCCAPTKLNAISVLYFDDNSNVILKKYRNMVVRACGCH 3F19155B36049278 p22003-pro_0000033867 Bone morphogenetic protein 5 BMP5 protein protein human Homo sapiens Human AANKRKNQNRNKSSSHQDSSRMSSVGDYNTSEQKQACKKHELYVSFRDLGWQDWIIAPEGYAAFYCDGECSFPLNAHMNATNHAIVQTLVHLMFPDHVPKPCCAPTKLNAISVLYFDDSSNVILKKYRNMVVRSCGCH 317 454 64886D623093E262 tnf11_human Tumor necrosis factor ligand superfamily member 11 TNFSF11 OPGL Osteoclast differentiation factor Osteoprotegerin ligand RANKL Receptor activator of nuclear factor kappa-B ligand TNF-related activation-induced cytokine TRANCE protein protein human Homo sapiens Human MRRASRDYTKYLRGSEEMGGGPGAPHEGPLHAPPPPAPHQPPAASRSMFVALLGLGLGQVVCSVALFFYFRAQMDPNRISEDGTHCIYRILRLHENADFQDTTLESQDTKLIPDSCRRIKQAFQGAVQKELQHIVGSQHIRAEKAMVDGSWLDLAKRSKLEAQPFAHLTINATDIPSGSHKVSLSSWYHDRGWAKISNMTFSNGKLIVNQDGFYYLYANICFRHHETSGDLATEYLQLMVYVTKTSIKIPSSHTLMKGGSTKYWSGNSEFHFYSINVGGFFKLRSGEEISIEVSNPSLLDPDQDATYFGAFKVRDID mint homomint 766176446348097F q6kf10-pro_0000042253 Growth/differentiation factor 6 GDF6 BMP13 GDF16 Bone morphogenetic protein 13 Growth/differentiation factor 16 protein protein human Homo sapiens Human TAFASRHGKRHGKKSRLRCSKKPLHVNFKELGWDDWIIAPLEYEAYHCEGVCDFPLRSHLEPTNHAIIQTLMNSMDPGSTPPSCCVPTKLTPISILYIDAGNNVVYKQYEDMVVESCGCR 336 455 7179C739375C9804 p43026-pro_0000033913 Growth/differentiation factor 5 GDF5 BMP14 CDMP1 Bone morphogenetic protein 14 Cartilage-derived morphogenetic protein 1 Lipopolysaccharide-associated protein 4 Radotermin protein protein human Homo sapiens Human APLATRQGKRPSKNLKARCSRKALHVNFKDMGWDDWIIAPLEYEAFHCEGLCEFPLRSHLEPTNHAVIQTLMNSMDPESTPPTCCVPTRLSPISILFIDSANNVVYKQYEDMVVESCGCR 382 501 E6F29EF85E97F3E6 tsg6_human Tumor necrosis factor-inducible gene 6 protein TSG6 TNFAIP6 Hyaluronate-binding protein TNF-stimulated gene 6 protein Tumor necrosis factor alpha-induced protein 6 protein protein human Homo sapiens Human MIILIYLFLLLWEDTQGWGFKDGIFHNSIWLERAAGVYHREARSGKYKLTYAEAKAVCEFEGGHLATYKQLEAARKIGFHVCAAGWMAKGRVGYPIVKPGPNCGFGKTGIIDYGIRLNRSERWDAYCYNPHAKECGGVFTDPKQIFKSPGFPNEYEDNQICYWHIRLKYGQRIHLSFLDFDLEDDPGCLADYVEIYDSYDDVHGFVGRYCGDELPDDIISTGNVMTLKFLSDASVTAGGFQIKYVAMDPVSKSSQGKNTSTTSTGNKNFLAGRFSHL E2B3AEB76353A782 p12644-pro_0000033857 Bone morphogenetic protein 4 BMP4 BMP2B DVR4 Bone morphogenetic protein 2B protein protein human Homo sapiens Human SPKHHSQRARKKNKNCRRHSLYVDFSDVGWNDWIVAPPGYQAFYCHGDCPFPLADHLNSTNHAIVQTLVNSVNSSIPKACCVPTELSAISMLYLDEYDKVVLKNYQEMVVEGCGCR 293 408 9958E94C3C2610DC p12643-pro_0000033825 Bone morphogenetic protein 2 BMP2 BMP2A Bone morphogenetic protein 2A protein protein human Homo sapiens Human QAKHKQRKRLKSSCKRHPLYVDFSDVGWNDWIVAPPGYHAFYCHGECPFPLADHLNSTNHAIVQTLVNSVNSKIPKACCVPTELSAISMLYLDENEKVVLKNYQDMVVEGCGCR 283 396 8F0C175EE6D508B5 p18075-pro_0000033877 Bone morphogenetic protein 7 Osteogenic protein 1 BMP7 OP1 protein protein human Homo sapiens Human STGSKQRSQNRSKTPKNQEALRMANVAENSSSDQRQACKKHELYVSFRDLGWQDWIIAPEGYAAYYCEGECAFPLNSYMNATNHAIVQTLVHFINPETVPKPCCAPTQLNAISVLYFDDSSNVILKKYRNMVVRACGCH 293 431 1B7EBF3043374581 bmp13-tsg6-1 2 6 unspecified role unspecified role bait bait purified purified ecoli Escherichia coli (strain K12) 8 unspecified role unspecified role prey prey purified purified c_terminus v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range c_terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range drome-schneider_2 Drosophila melanogaster late embryo cells Fruit fly schneider_2 Established from the late embryo of Drosophila melanogaster Mixture of diploid and tetraploid. Originally had some XY cells now predominantly XX. direct interaction direct interaction 6,7,8 The Link domain (aas36-133 of pre-protein) and Cub_C (aas 129-277) region of TSG6 have a much lower level of binding as compared to full-length TSG-6. gdf6-tnfaip6-1 2 11 unspecified role unspecified role bait bait purified purified crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. 8 unspecified role unspecified role prey prey purified purified c_terminus v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range c_terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range drome-schneider_2 Drosophila melanogaster late embryo cells Fruit fly schneider_2 Established from the late embryo of Drosophila melanogaster Mixture of diploid and tetraploid. Originally had some XY cells now predominantly XX. direct interaction direct interaction 6-2 bmp2-tnfaip6-1 2 10 unspecified role unspecified role bait bait purified purified n_terminus met L-methionine residue (2S)-2-amino-4-(methylsulfanyl)butanoic acid 2-amino-4-(methylthio)butanoic acid 2-amino-4-(methylthio)butyric acid 2-azanyl-4-(methylsulfanyl)butanoic acid L-(-)-methionine L-methionine S-methyl-L-homocysteine alpha-amino-gamma-methylmercaptobutyric acid alpha-amino-gamma-methylthiobutyric acid gamma-methylthio-alpha-aminobutyric acid Met n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) 8 unspecified role unspecified role prey prey purified purified c_terminus v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range c_terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range drome-schneider_2 Drosophila melanogaster late embryo cells Fruit fly schneider_2 Established from the late embryo of Drosophila melanogaster Mixture of diploid and tetraploid. Originally had some XY cells now predominantly XX. direct interaction direct interaction 6, 7,8 The Link domain (aas36-133 of pre-protein) and Cub_C (aas 129-277) region of TSG6 have a much lower level of binding as compared to full-length TSG-6. bmp5-tnfaip6-1 2 4 unspecified role unspecified role bait bait purified purified crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. 8 unspecified role unspecified role prey prey purified purified c_terminus v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range c_terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range drome-schneider_2 Drosophila melanogaster late embryo cells Fruit fly schneider_2 Established from the late embryo of Drosophila melanogaster Mixture of diploid and tetraploid. Originally had some XY cells now predominantly XX. direct interaction direct interaction 6-4 gdf6-tnfaip6-2 1 6 unspecified role unspecified role bait bait purified purified ecoli Escherichia coli (strain K12) 8 unspecified role unspecified role prey prey purified purified c_terminus v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range c_terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range drome-schneider_2 Drosophila melanogaster late embryo cells Fruit fly schneider_2 Established from the late embryo of Drosophila melanogaster Mixture of diploid and tetraploid. Originally had some XY cells now predominantly XX. direct interaction direct interaction Table 2 bmp2-tnfaip6-2 1 10 unspecified role unspecified role bait bait purified purified n_terminus met L-methionine residue (2S)-2-amino-4-(methylsulfanyl)butanoic acid 2-amino-4-(methylthio)butanoic acid 2-amino-4-(methylthio)butyric acid 2-azanyl-4-(methylsulfanyl)butanoic acid L-(-)-methionine L-methionine S-methyl-L-homocysteine alpha-amino-gamma-methylmercaptobutyric acid alpha-amino-gamma-methylthiobutyric acid gamma-methylthio-alpha-aminobutyric acid Met n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) 8 unspecified role unspecified role prey prey purified purified c_terminus v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range c_terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range drome-schneider_2 Drosophila melanogaster late embryo cells Fruit fly schneider_2 Established from the late embryo of Drosophila melanogaster Mixture of diploid and tetraploid. Originally had some XY cells now predominantly XX. direct interaction direct interaction Table 2 bmp4-tnfaip6-1 2 9 unspecified role unspecified role bait bait purified purified mouse-nso NSO-mouse myeloma subclone of NS-1 ns0 NS0 Mouse Myeloma subclone of NS-1 http://www.hpacultures.org.uk/products/celllines/generalcell/detail.jsp?refId=85110503&collection=ecacc_gc from ecacc 8 unspecified role unspecified role prey prey purified purified c_terminus v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range c_terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range drome-schneider_2 Drosophila melanogaster late embryo cells Fruit fly schneider_2 Established from the late embryo of Drosophila melanogaster Mixture of diploid and tetraploid. Originally had some XY cells now predominantly XX. direct interaction direct interaction 6-5 bmp6-tnfaip6-1 2 3 unspecified role unspecified role bait bait purified purified region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain mouse-nso NSO-mouse myeloma subclone of NS-1 ns0 NS0 Mouse Myeloma subclone of NS-1 http://www.hpacultures.org.uk/products/celllines/generalcell/detail.jsp?refId=85110503&collection=ecacc_gc from ecacc 8 unspecified role unspecified role prey prey purified purified c_terminus v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range c_terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range drome-schneider_2 Drosophila melanogaster late embryo cells Fruit fly schneider_2 Established from the late embryo of Drosophila melanogaster Mixture of diploid and tetraploid. Originally had some XY cells now predominantly XX. direct interaction direct interaction 6-6 gdf5-tnfaip6-1 2 7 unspecified role unspecified role bait bait purified purified ecoli Escherichia coli (strain K12) 8 unspecified role unspecified role prey prey purified purified c_terminus v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range c_terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range drome-schneider_2 Drosophila melanogaster late embryo cells Fruit fly schneider_2 Established from the late embryo of Drosophila melanogaster Mixture of diploid and tetraploid. Originally had some XY cells now predominantly XX. direct interaction direct interaction 6,7i The Link domain (aas36-133 of pre-protein) and Cub_C (aas 129-277) region of TSG6 have a much lower level of binding as compared to full-length TSG-6. gdf6-tnfaip6-3 1 5 unspecified role unspecified role bait bait purified purified region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain O14788:p.Ala142Met mutation mutation certain certain sequence position certain certain certain sequence position certain A M Wed May 02 17:41:34 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ala142met' Tue Jun 30 16:42:15 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ala142Met' ecoli Escherichia coli (strain K12) 8 unspecified role unspecified role prey prey purified purified c_terminus v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range c_terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range drome-schneider_2 Drosophila melanogaster late embryo cells Fruit fly schneider_2 Established from the late embryo of Drosophila melanogaster Mixture of diploid and tetraploid. Originally had some XY cells now predominantly XX. direct interaction direct interaction Table 2 No result was obtained with CUB_C_TSG6 construct. bmp2-tnfaip6-3 1 10 unspecified role unspecified role bait bait purified purified n_terminus met L-methionine residue (2S)-2-amino-4-(methylsulfanyl)butanoic acid 2-amino-4-(methylthio)butanoic acid 2-amino-4-(methylthio)butyric acid 2-azanyl-4-(methylsulfanyl)butanoic acid L-(-)-methionine L-methionine S-methyl-L-homocysteine alpha-amino-gamma-methylmercaptobutyric acid alpha-amino-gamma-methylthiobutyric acid gamma-methylthio-alpha-aminobutyric acid Met n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) 8 unspecified role unspecified role prey prey purified purified link_domain binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain cub_c_region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) direct interaction direct interaction Table 2 gdf6-tnfaip6-4 1 6 unspecified role unspecified role bait bait purified purified ecoli Escherichia coli (strain K12) 8 unspecified role unspecified role prey prey purified purified link_domain binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain cub_c_region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) direct interaction direct interaction Table 2 tnfsf11-tnfaip6-1 2 5 unspecified role unspecified role bait bait purified purified region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain O14788:p.Ala142Met mutation mutation certain certain sequence position certain certain certain sequence position certain A M Wed May 02 16:57:32 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ala142met' Tue Jun 30 17:11:29 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ala142Met' ecoli Escherichia coli (strain K12) 8 unspecified role unspecified role prey prey purified purified link_domain binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) direct interaction direct interaction 8B No result was obtained with CUB_C_TSG6 construct. tnfsf11-tnfaip6-2 2 5 unspecified role unspecified role bait bait purified purified region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain O14788:p.Ala142Met mutation mutation certain certain sequence position certain certain certain sequence position certain A M Wed May 02 20:01:54 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ala142met' Tue Jun 30 18:03:15 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ala142Met' ecoli Escherichia coli (strain K12) 8 unspecified role unspecified role prey prey c_terminus v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range c_terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range drome-schneider_2 Drosophila melanogaster late embryo cells Fruit fly schneider_2 Established from the late embryo of Drosophila melanogaster Mixture of diploid and tetraploid. Originally had some XY cells now predominantly XX. direct interaction direct interaction 8 A/B No significant result was obtained with CUB_C_TSG6 or Link constructs. tnfsf11-tnfaip6-3 2 5 unspecified role unspecified role prey prey purified purified region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain O14788:p.Ala142Met mutation mutation certain certain sequence position certain certain certain sequence position certain A M Wed May 02 19:00:32 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ala142met' Tue Jun 30 17:08:23 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ala142Met' region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 8 unspecified role unspecified role bait bait purified purified cub_c_region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain link_Region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) direct interaction direct interaction 8C the extent of binding to Link_TSG6 and CUB_C_TSG6 was lower than that seen for the full-length protein. gdf5-tnfaip6-2 1 7 unspecified role unspecified role bait bait purified purified ecoli Escherichia coli (strain K12) 8 unspecified role unspecified role prey prey purified purified c_terminus v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range c_terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range drome-schneider_2 Drosophila melanogaster late embryo cells Fruit fly schneider_2 Established from the late embryo of Drosophila melanogaster Mixture of diploid and tetraploid. Originally had some XY cells now predominantly XX. direct interaction direct interaction Table 2 gdf5-tnfaip6-3 1 7 unspecified role unspecified role bait bait purified purified ecoli Escherichia coli (strain K12) 8 unspecified role unspecified role prey prey purified purified link_domain binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain cub_c_region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) direct interaction direct interaction Table 2i