IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ The yeast ribosome synthesis factor Emg1 is a novel member of the superfamily of alpha/beta knot fold methyltransferases. The yeast ribosome synthesis factor Emg1 is a novel member of the superfamily of alpha/beta knot fold methyltransferases. Nucleic acids research 2008 imex curation Leulliot N., Bohnsack MT., Graille M., Tollervey D., Van Tilbeurgh H. nicolas.leulliot@u-psud.fr Only protein-protein interactions in vitro In vitro pull down pull down weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Leulliot N., Bohnsack MT., Graille M., Tollervey D., Van Tilbeurgh H. nicolas.leulliot@u-psud.fr Accepted 2017-FEB-27 AT 08:59 GMT AT 08:59 GMT by ORCHARD Nucleic acids research 2008 imex curation Sorry for replay so late. I have corrected EBI-11664448 accordingly and override its participant detection m. The yeast ribosome synthesis factor Emg1 is a novel member of the superfamily of alpha/beta knot fold methyltransferases. The yeast ribosome synthesis factor Emg1 is a novel member of the superfamily of alpha/beta knot fold methyltransferases. Nucleic acids research 2008 imex curation Leulliot N., Bohnsack MT., Graille M., Tollervey D., Van Tilbeurgh H. nicolas.leulliot@u-psud.fr Only protein-protein interactions in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Leulliot N., Bohnsack MT., Graille M., Tollervey D., Van Tilbeurgh H. nicolas.leulliot@u-psud.fr Accepted 2017-FEB-27 AT 08:59 GMT AT 08:59 GMT by ORCHARD Nucleic acids research 2008 imex curation Sorry for replay so late. I have corrected EBI-11664448 accordingly and override its participant detection m. rrna 18s yeast Ribosomal RNA 18S Yeast rrna ribosomal rna rRNA rrna yeast Saccharomyces cerevisiae Baker's yeast UAUCUGGUUGAUCCUGCCAGUAGUCAUAUGCUUGUCUCAAAGAUUAAGCCAUGCAUGUCUAAGUAUAAGCAAUUUAUACAGUGAAACUGCGAAUGGCUCAUUAAAUCAGUUAUCGUUUAUUUGAUAGUUCCUUUACUACAUGGUAUAACUGUGGUAAUUCUAGAGCUAAUACAUGCUUAAAAUCUCGACCCUUUGGAAGAGAUGUAUUUAUUAGAUAAAAAAUCAAUGUCUUCGGACUCUUUGAUGAUUCAUAAUAACUUUUCGAAUCGCAUGGCCUUGUGCUGGCGAUGGUUCAUUCAAAUUUCUGCCCUAUCAACUUUCGAUGGUAGGAUAGUGGCCUACCAUGGUUUCAACGGGUAACGGGGAAUAAGGGUUCGAUUCCGGAGAGGGAGCCUGAGAAACGGCUACCACAUCCAAGGAAGGCAGCAGGCGCGCAAAUUACCCAAUCCUAAUUCAGGGAGGUAGUGACAAUAAAUAACGAUACAGGGCCCAUUCGGGUCUUGUAAUUGGAAUGAGUACAAUGUAAAUACCUUAACGAGGAACAAUUGGAGGGCAAGUCUGGUGCCAGCAGCCGCGGUAAUUCCAGCUCCAAUAGCGUAUAUUAAAGUUGUUGCAGUUAAAAAGCUCGUAGUUGAACUUUGGGCCCGGUUGGCCGGUCCGAUUUUUUCGUGUACUGGAUUUCCAACGGGGCCUUUCCUUCUGGCUAACCUUGAGUCCUUGUGGCUCUUGGCGAACCAGGACUUUUACUUUGAAAAAAUUAGAGUGUUCAAAGCAGGCGUAUUGCUCGAAUAUAUUAGCAUGGAAUAAUAGAAUAGGACGUUUGGUUCUAUUUUGUUGGUUUCUAGGACCAUCGUAAUGAUUAAUAGGGACGGUCGGGGGCAUCAGUAUUCAAUUGUCAGAGGUGAAAUUCUUGGAUUUAUUGAAGACUAACUACUGCGAAAGCAUUUGCCAAGGACGUUUUCAUUAAUCAAGAACGAAAGUUAGGGGAUCGAAGAUGAUCAGAUACCGUCGUAGUCUUAACCAUAAACUAUGCCGACUAGGGAUCGGGUGGUGUUUUUUUAAUGACCCACUCGGCACCUUACGAGAAAUCAAAGUCUUUGGGUUCUGGGGGGAGUAUGGUCGCAAGGCUGAAACUUAAAGGAAUUGACGGAAGGGCACCACCAGGAGUGGAGCCUGCGGCUUAAUUUGACUCAACACGGGGAAACUCACCAGGUCCAGACACAAUAAGGAUUGACAGAUUGAGAGCUCUUUCUUGAUUUUGUGGGUGGUGGUGCAUGGCCGUUCUUAGUUGGUGGAGUGAUUUGUCUGCUUAAUUGCGAUAACGAACGAGACCUUAACCUACUAAAUAGUGGUGCUAGCAUUUGCUGGUUAUCCACUUCUUAGAGGGACUAUCGGUUUCAAGCCGAUGGAAGUUUGAGGCAAUAACAGGUCUGUGAUGCCCUUAGACGUUCUGGGCCGCACGCGCGCUACACUGACGGAGCCAGCGAGUCUAACCUUGGCCGAGAGGUCUUGGUAAUCUUGUGAAACUCCGUCGUGCUGGGGAUAGAGCAUUGUAAUUAUUGCUCUUCAACGAGGAAUUCCUAGUAAGCGCAAGUCAUCAGCUUGCGUUGAUUACGUCCCUGCCCUUUGUACACACCGCCCGUCGCUAGUACCGAUUGAAUGGCUUAGUGAGGCCUCAGGAUCUGCUUAGAGAAGGGGGCAACUCCAUCUCAGAGCGGAGAAUUUGGACAAACUUGGUCAUUUAGAGGAACUAAAAGUCGUAACAAGGUUUCCGUAGGUGAACCUGCGGAAGGAUCAUUA nep1_yeast Ribosomal RNA small subunit methyltransferase NEP1 EMG1 NEP1 YLR186W L9470.5 Nucleolar essential protein 1 18S rRNA (pseudouridine(1189)-N1)-methyltransferase Essential for mitotic growth protein 1 protein protein yeast Saccharomyces cerevisiae Baker's yeast MVEDSRVRDALKGGDQKALPASLVPQAPPVLTSKDKITKRMIVVLAMASLETHKISSNGPGGDKYVLLNCDDHQGLLKKMGRDISEARPDITHQCLLTLLDSPINKAGKLQVYIQTSRGILIEVNPTVRIPRTFKRFSGLMVQLLHKLSIRSVNSEEKLLKVIKNPITDHLPTKCRKVTLSFDAPVIRVQDYIEKLDDDESICVFVGAMARGKDNFADEYVDEKVGLSNYPLSASVACSKFCHGAEDAWNIL 4A8FCDA812051AD1 4A8FCDA812051AD1 s-adenosyl-l-methionine S adenosyl Methionine small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) emg1-rrna 18s yeast-1 1 3 unspecified role unspecified role prey prey label 32p radiolabel 32p radiolabel P32 32P 32P P32 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 4 unspecified role unspecified role bait bait tag ZZ-His tag tandem tag tandem tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range Q06287:p.Arg88Asp mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain R D Wed May 02 20:26:57 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg88asp' Tue Jun 30 16:29:28 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg88Asp' ecoli Escherichia coli (strain K12) direct interaction direct interaction Fig4A emg1-emg1-1 1 4 unspecified role unspecified role prey prey tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) 4 predetermined predetermined participant predetermined weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra unspecified role unspecified role bait bait tag ZZ-His tag tandem tag tandem tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range Q06287:p.Arg88Asp mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain R D Wed May 02 17:14:29 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg88asp' Tue Jun 30 18:07:22 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg88Asp' ecoli Escherichia coli (strain K12) direct interaction direct interaction Fig4B emg1-1 2 4 unspecified role unspecified role neutral component neutral component tag ZZ-His tag tandem tag tandem tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range direct interaction direct interaction Fig4C emg1-s_adenosyl_l_methionine_1-1 1 4 unspecified role unspecified role bait bait tag ZZ-His tag tandem tag tandem tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range Q06287:p.Asp214Arg mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain D R Fri Jul 22 10:59:04 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'asp214arg,lys232ser,ala237asp' Wed May 02 19:28:49 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp214arg' Tue Jun 30 18:35:54 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp214Arg' Q06287:p.Leu232Ser mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain L S Wed May 02 14:27:36 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu232ser' Tue Jun 30 20:14:59 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu232Ser' Q06287:p.Ala237Asp mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain A D Wed May 02 14:51:41 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ala237asp' Tue Jun 30 16:57:58 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ala237Asp' ecoli Escherichia coli (strain K12) 5 tag visualisation tag visualisation weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra unspecified role unspecified role prey prey label 3h radiolabel 3h radiolabel 3H tritium H3 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined direct interaction direct interaction Fig5