matrixdb Institut de Biologie et de Chimie des Prot\u00c3\u00a9ines, 7, passage du Vercors 69 367 Lyon cedex 07 FRANCE matrixdb@ibcp.fr http://matrixdb.ibcp.fr Interaction of the small proteoglycan decorin with fibronectin. Involvement of the sequence NKISK of the core protein. Interaction of the small proteoglycan decorin with fibronectin. Involvement of the sequence NKISK of the core protein. The Biochemical journal 1991 imex curation Schmidt G., Hausser H., Kresse H. kresse@uni-muenster.de 22-Jun-2016: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro solid phase assay solid phase assay antibody detection identification by antibody antibody detection Schmidt G., Hausser H., Kresse H. kresse@uni-muenster.de Accepted 2016-MAY-11 AT 12:39 BST AT 12:39 BST by ORCHARD The Biochemical journal 1991 Only protein-protein interactions imex curation imex curation Interaction of the small proteoglycan decorin with fibronectin. Involvement of the sequence NKISK of the core protein. Interaction of the small proteoglycan decorin with fibronectin. Involvement of the sequence NKISK of the core protein. The Biochemical journal 1991 imex curation Schmidt G., Hausser H., Kresse H. kresse@uni-muenster.de 22-Jun-2016: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro affinity chrom affinity chromatography technology Affinity purification Affinity purification affinity chrom tag visualisation tag visualisation Schmidt G., Hausser H., Kresse H. kresse@uni-muenster.de Accepted 2016-MAY-11 AT 12:39 BST AT 12:39 BST by ORCHARD The Biochemical journal 1991 Only protein-protein interactions imex curation imex curation Interaction of the small proteoglycan decorin with fibronectin. Involvement of the sequence NKISK of the core protein. Interaction of the small proteoglycan decorin with fibronectin. Involvement of the sequence NKISK of the core protein. The Biochemical journal 1991 imex curation Schmidt G., Hausser H., Kresse H. kresse@uni-muenster.de 22-Jun-2016: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro solid phase assay solid phase assay tag visualisation tag visualisation Schmidt G., Hausser H., Kresse H. kresse@uni-muenster.de Accepted 2016-MAY-11 AT 12:39 BST AT 12:39 BST by ORCHARD The Biochemical journal 1991 Only protein-protein interactions imex curation imex curation finc_human Fibronectin FN1 FN Cold-insoluble globulin protein protein human Homo sapiens Human MLRGPGPGLLLLAVQCLGTAVPSTGASKSKRQAQQMVQPQSPVAVSQSKPGCYDNGKHYQINQQWERTYLGNALVCTCYGGSRGFNCESKPEAEETCFDKYTGNTYRVGDTYERPKDSMIWDCTCIGAGRGRISCTIANRCHEGGQSYKIGDTWRRPHETGGYMLECVCLGNGKGEWTCKPIAEKCFDHAAGTSYVVGETWEKPYQGWMMVDCTCLGEGSGRITCTSRNRCNDQDTRTSYRIGDTWSKKDNRGNLLQCICTGNGRGEWKCERHTSVQTTSSGSGPFTDVRAAVYQPQPHPQPPPYGHCVTDSGVVYSVGMQWLKTQGNKQMLCTCLGNGVSCQETAVTQTYGGNSNGEPCVLPFTYNGRTFYSCTTEGRQDGHLWCSTTSNYEQDQKYSFCTDHTVLVQTRGGNSNGALCHFPFLYNNHNYTDCTSEGRRDNMKWCGTTQNYDADQKFGFCPMAAHEEICTTNEGVMYRIGDQWDKQHDMGHMMRCTCVGNGRGEWTCIAYSQLRDQCIVDDITYNVNDTFHKRHEEGHMLNCTCFGQGRGRWKCDPVDQCQDSETGTFYQIGDSWEKYVHGVRYQCYCYGRGIGEWHCQPLQTYPSSSGPVEVFITETPSQPNSHPIQWNAPQPSHISKYILRWRPKNSVGRWKEATIPGHLNSYTIKGLKPGVVYEGQLISIQQYGHQEVTRFDFTTTSTSTPVTSNTVTGETTPFSPLVATSESVTEITASSFVVSWVSASDTVSGFRVEYELSEEGDEPQYLDLPSTATSVNIPDLLPGRKYIVNVYQISEDGEQSLILSTSQTTAPDAPPDTTVDQVDDTSIVVRWSRPQAPITGYRIVYSPSVEGSSTELNLPETANSVTLSDLQPGVQYNITIYAVEENQESTPVVIQQETTGTPRSDTVPSPRDLQFVEVTDVKVTIMWTPPESAVTGYRVDVIPVNLPGEHGQRLPISRNTFAEVTGLSPGVTYYFKVFAVSHGRESKPLTAQQTTKLDAPTNLQFVNETDSTVLVRWTPPRAQITGYRLTVGLTRRGQPRQYNVGPSVSKYPLRNLQPASEYTVSLVAIKGNQESPKATGVFTTLQPGSSIPPYNTEVTETTIVITWTPAPRIGFKLGVRPSQGGEAPREVTSDSGSIVVSGLTPGVEYVYTIQVLRDGQERDAPIVNKVVTPLSPPTNLHLEANPDTGVLTVSWERSTTPDITGYRITTTPTNGQQGNSLEEVVHADQSSCTFDNLSPGLEYNVSVYTVKDDKESVPISDTIIPEVPQLTDLSFVDITDSSIGLRWTPLNSSTIIGYRITVVAAGEGIPIFEDFVDSSVGYYTVTGLEPGIDYDISVITLINGGESAPTTLTQQTAVPPPTDLRFTNIGPDTMRVTWAPPPSIDLTNFLVRYSPVKNEEDVAELSISPSDNAVVLTNLLPGTEYVVSVSSVYEQHESTPLRGRQKTGLDSPTGIDFSDITANSFTVHWIAPRATITGYRIRHHPEHFSGRPREDRVPHSRNSITLTNLTPGTEYVVSIVALNGREESPLLIGQQSTVSDVPRDLEVVAATPTSLLISWDAPAVTVRYYRITYGETGGNSPVQEFTVPGSKSTATISGLKPGVDYTITVYAVTGRGDSPASSKPISINYRTEIDKPSQMQVTDVQDNSISVKWLPSSSPVTGYRVTTTPKNGPGPTKTKTAGPDQTEMTIEGLQPTVEYVVSVYAQNPSGESQPLVQTAVTNIDRPKGLAFTDVDVDSIKIAWESPQGQVSRYRVTYSSPEDGIHELFPAPDGEEDTAELQGLRPGSEYTVSVVALHDDMESQPLIGTQSTAIPAPTDLKFTQVTPTSLSAQWTPPNVQLTGYRVRVTPKEKTGPMKEINLAPDSSSVVVSGLMVATKYEVSVYALKDTLTSRPAQGVVTTLENVSPPRRARVTDATETTITISWRTKTETITGFQVDAVPANGQTPIQRTIKPDVRSYTITGLQPGTDYKIYLYTLNDNARSSPVVIDASTAIDAPSNLRFLATTPNSLLVSWQPPRARITGYIIKYEKPGSPPREVVPRPRPGVTEATITGLEPGTEYTIYVIALKNNQKSEPLIGRKKTDELPQLVTLPHPNLHGPEILDVPSTVQKTPFVTHPGYDTGNGIQLPGTSGQQPSVGQQMIFEEHGFRRTTPPTTATPIRHRPRPYPPNVGEEIQIGHIPREDVDYHLYPHGPGLNPNASTGQEALSQTTISWAPFQDTSEYIISCHPVGTDEEPLQFRVPGTSTSATLTGLTRGATYNVIVEALKDQQRHKVREEVVTVGNSVNEGLNQPTDDSCFDPYTVSHYAVGDEWERMSESGFKLLCQCLGFGSGHFRCDSSRWCHDNGVNYKIGEKWDRQGENGQMMSCTCLGNGKGEFKCDPHEATCYDDGKTYHVGEQWQKEYLGAICSCTCFGGQRGWRCDNCRRPGGEPSPEGTTGQSYNQYSQRYHQRTNTNVNCPIECFMPLDVQADREDSRE DIP protein P02751 original sequence version: 119 DIP protein P02751 original sequence version: 131 DIP protein P02751 original sequence version: 154 DIP protein P02751 original sequence version: 156 DIP protein P02751 original sequence version: 157 DIP protein P02751 original sequence version: 155 DIP protein P02751 original sequence version: 194 6C436A7A5FEE6DEB pgs2_human Decorin DCN SLRR1B Bone proteoglycan II PG-S2 PG40 protein protein human Homo sapiens Human MKATIILLLLAQVSWAGPFQQRGLFDFMLEDEASGIGPEVPDDRDFEPSLGPVCPFRCQCHLRVVQCSDLGLDKVPKDLPPDTTLLDLQNNKITEIKDGDFKNLKNLHALILVNNKISKVSPGAFTPLVKLERLYLSKNQLKELPEKMPKTLQELRAHENEITKVRKVTFNGLNQMIVIELGTNPLKSSGIENGAFQGMKKLSYIRIADTNITSIPQGLPPSLTELHLDGNKISRVDAASLKGLNNLAKLGLSFNSISAVDNGSLANTPHLRELHLDNNKLTRVPGGLAEHKYIQVVYLHNNNISVVGSSDFCPPGHNTKKASYSGVSLFSNPVQYWEIQPSTFRCVYVRSAIQLGNYK FF511E871A1A52DD dcn-fn1-1 3 4 unspecified role unspecified role bait bait human-plasma homo sapiens blood plasma plasma blood plasma 5 unspecified role unspecified role prey prey Isotope label 35S methionine 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human-skin Homo sapiens skin skin direct interaction direct interaction Figure 1 fn1-dcn-1 3 5 unspecified role unspecified role prey prey Isotope label 35S methionine 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined PTM Dermatan sulfate dermatan 4-sulfate d-glucuronosyl-d-galactosyl-d-galactosyl-d-xylosyl-l-serine dermatan 4-sulfate D-glucuronosyl-D-galactosyl-D-galactosyl-D-xylosyl-L-serine beta-heparin chondroitin sulfate B dermatan 4-sulfate D-glucuronosyl-D-galactosyl-D-galactosyl-D-xylosyl-L-serine poly[beta-1,4-L-idopyranuronosyl-alpha-1,3-(2-acetamido-2-deoxy-4-sulfate D-galactosyl)]-beta-1,4-D-glucopyranuronosyl-beta-1,3-D-galactosyl-beta-1,3-D-galactosyl-beta-1,4-D-xylosyl-beta-1,3-L-serine undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human-skin Homo sapiens skin skin 4 unspecified role unspecified role bait bait human-plasma homo sapiens blood plasma plasma blood plasma direct interaction direct interaction Figure 1 dcn-dcn-1 3 5 competitor competitor competitor neutral component neutral component Binding region NKISK sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain chemical synthesis Chemical synthesis (Chemical synthesis) 5 unspecified role unspecified role prey prey Isotope label 35S methionine 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human-skin Homo sapiens skin skin 4 unspecified role unspecified role bait bait human-plasma homo sapiens blood plasma plasma blood plasma association association Figure 1 dcn-fn1-2 2 5 unspecified role unspecified role prey prey Isotope label 3H leucine 3h radiolabel 3h radiolabel 3H tritium H3 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human-skin Homo sapiens skin skin 4 unspecified role unspecified role bait bait human-plasma homo sapiens blood plasma plasma blood plasma direct interaction direct interaction Figure 2 dcn-fn1-3 2 5 unspecified role unspecified role prey prey region 3h radiolabel 3h radiolabel 3H tritium H3 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined PTM Dermatan sulfate dermatan 4-sulfate d-glucuronosyl-d-galactosyl-d-galactosyl-d-xylosyl-l-serine dermatan 4-sulfate D-glucuronosyl-D-galactosyl-D-galactosyl-D-xylosyl-L-serine beta-heparin chondroitin sulfate B dermatan 4-sulfate D-glucuronosyl-D-galactosyl-D-galactosyl-D-xylosyl-L-serine poly[beta-1,4-L-idopyranuronosyl-alpha-1,3-(2-acetamido-2-deoxy-4-sulfate D-galactosyl)]-beta-1,4-D-glucopyranuronosyl-beta-1,3-D-galactosyl-beta-1,3-D-galactosyl-beta-1,4-D-xylosyl-beta-1,3-L-serine undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human-skin Homo sapiens skin skin 4 unspecified role unspecified role bait bait human-plasma homo sapiens blood plasma plasma blood plasma direct interaction direct interaction Figure 2 dcn-dcn-2 1 5 unspecified role unspecified role prey prey human-skin Homo sapiens skin skin 5 competitor competitor competitor prey prey Binding region NKISK sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain chemical synthesis Chemical synthesis (Chemical synthesis) 4 unspecified role unspecified role bait bait human-plasma homo sapiens blood plasma plasma blood plasma association association Figure 3 dcn-dcn-3 1 5 competitor competitor competitor prey prey Binding region NKISK sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain chemical synthesis Chemical synthesis (Chemical synthesis) 5 unspecified role unspecified role prey prey PTM Dermatan sulfate dermatan 4-sulfate d-glucuronosyl-d-galactosyl-d-galactosyl-d-xylosyl-l-serine dermatan 4-sulfate D-glucuronosyl-D-galactosyl-D-galactosyl-D-xylosyl-L-serine beta-heparin chondroitin sulfate B dermatan 4-sulfate D-glucuronosyl-D-galactosyl-D-galactosyl-D-xylosyl-L-serine poly[beta-1,4-L-idopyranuronosyl-alpha-1,3-(2-acetamido-2-deoxy-4-sulfate D-galactosyl)]-beta-1,4-D-glucopyranuronosyl-beta-1,3-D-galactosyl-beta-1,3-D-galactosyl-beta-1,4-D-xylosyl-beta-1,3-L-serine undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human-skin Homo sapiens skin skin 4 unspecified role unspecified role bait bait human-plasma homo sapiens blood plasma plasma blood plasma association association Figure 4 fn1-dcn-2 1 5 unspecified role unspecified role prey prey human-skin Homo sapiens skin skin 4 unspecified role unspecified role bait bait human-plasma homo sapiens blood plasma plasma blood plasma association association Figure 3 fn1-dcn-3 1 5 unspecified role unspecified role prey prey PTM Dermatan sulfate dermatan 4-sulfate d-glucuronosyl-d-galactosyl-d-galactosyl-d-xylosyl-l-serine dermatan 4-sulfate D-glucuronosyl-D-galactosyl-D-galactosyl-D-xylosyl-L-serine beta-heparin chondroitin sulfate B dermatan 4-sulfate D-glucuronosyl-D-galactosyl-D-galactosyl-D-xylosyl-L-serine poly[beta-1,4-L-idopyranuronosyl-alpha-1,3-(2-acetamido-2-deoxy-4-sulfate D-galactosyl)]-beta-1,4-D-glucopyranuronosyl-beta-1,3-D-galactosyl-beta-1,3-D-galactosyl-beta-1,4-D-xylosyl-beta-1,3-L-serine undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human-skin Homo sapiens skin skin 4 unspecified role unspecified role bait bait human-plasma homo sapiens blood plasma plasma blood plasma association association Figure 4