IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ The Cfd1-Nbp35 complex acts as a scaffold for iron-sulfur protein assembly in the yeast cytosol. The Cfd1-Nbp35 complex acts as a scaffold for iron-sulfur protein assembly in the yeast cytosol. Nature chemical biology 2007 imex curation Netz DJ., Pierik AJ., Stümpfig M., Mühlenhoff U., Lill R. lill@staff.uni-marburg.de 22-Jun-2016: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro electron resonance electron resonance predetermined predetermined participant predetermined Netz DJ., Pierik AJ., Stümpfig M., Mühlenhoff U., Lill R. lill@staff.uni-marburg.de Accepted 2016-MAY-27 AT 08:27 BST AT 08:27 BST by MGT Nature chemical biology 2007 imex curation EBI-11665876 is lane 6 of 1C. All else done.; I found a +1 charge - it was a child of the one I'd picked - but I've only used it for the CFD1 entry where the statement is made. Otherwise I've played safe and left it as the parent.; I've added the comment only to the full-length CFD1; Curated complex link worked for me - must have been a glitch. The Cfd1-Nbp35 complex acts as a scaffold for iron-sulfur protein assembly in the yeast cytosol. The Cfd1-Nbp35 complex acts as a scaffold for iron-sulfur protein assembly in the yeast cytosol. Nature chemical biology 2007 imex curation Netz DJ., Pierik AJ., Stümpfig M., Mühlenhoff U., Lill R. lill@staff.uni-marburg.de 22-Jun-2016: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Netz DJ., Pierik AJ., Stümpfig M., Mühlenhoff U., Lill R. lill@staff.uni-marburg.de Accepted 2016-MAY-27 AT 08:27 BST AT 08:27 BST by MGT Nature chemical biology 2007 imex curation EBI-11665876 is lane 6 of 1C. All else done.; I found a +1 charge - it was a child of the one I'd picked - but I've only used it for the CFD1 entry where the statement is made. Otherwise I've played safe and left it as the parent.; I've added the comment only to the full-length CFD1; Curated complex link worked for me - must have been a glitch. The Cfd1-Nbp35 complex acts as a scaffold for iron-sulfur protein assembly in the yeast cytosol. The Cfd1-Nbp35 complex acts as a scaffold for iron-sulfur protein assembly in the yeast cytosol. Nature chemical biology 2007 imex curation Netz DJ., Pierik AJ., Stümpfig M., Mühlenhoff U., Lill R. lill@staff.uni-marburg.de 22-Jun-2016: Contacted by IntAct-Help. Only protein-protein interactions yeast Saccharomyces cerevisiae Baker's yeast affinity chrom affinity chromatography technology Affinity purification Affinity purification affinity chrom anti tag western anti tag western blot anti tag western Netz DJ., Pierik AJ., Stümpfig M., Mühlenhoff U., Lill R. lill@staff.uni-marburg.de Accepted 2016-MAY-27 AT 08:27 BST AT 08:27 BST by MGT Nature chemical biology 2007 imex curation EBI-11665876 is lane 6 of 1C. All else done.; I found a +1 charge - it was a child of the one I'd picked - but I've only used it for the CFD1 entry where the statement is made. Otherwise I've played safe and left it as the parent.; I've added the comment only to the full-length CFD1; Curated complex link worked for me - must have been a glitch. The Cfd1-Nbp35 complex acts as a scaffold for iron-sulfur protein assembly in the yeast cytosol. The Cfd1-Nbp35 complex acts as a scaffold for iron-sulfur protein assembly in the yeast cytosol. Nature chemical biology 2007 imex curation Netz DJ., Pierik AJ., Stümpfig M., Mühlenhoff U., Lill R. lill@staff.uni-marburg.de 22-Jun-2016: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro biochemical biochemical predetermined predetermined participant predetermined Netz DJ., Pierik AJ., Stümpfig M., Mühlenhoff U., Lill R. No details of purification of Leu1 but it must be pure, otherwise an assay like this just wouldn't work Purified recombinant Cfd1, Nbp35, and the Cfd1–Nbp35 complex were tested for their ability to transfer, and thus activate, their bound [Fe-S] clusters to the apoform of yeast isopropylmalate isomerase (apo-Leu1). Under strictly anoxic conditions variable amounts of Cfd1, Nbp35 and Cfd1–Nbp35 complex were added to apo-Leu1. Samples were assayed for isopropylmalate isomerase enzyme activity lill@staff.uni-marburg.de Accepted 2016-MAY-27 AT 08:27 BST AT 08:27 BST by MGT Nature chemical biology 2007 imex curation EBI-11665876 is lane 6 of 1C. All else done.; I found a +1 charge - it was a child of the one I'd picked - but I've only used it for the CFD1 entry where the statement is made. Otherwise I've played safe and left it as the parent.; I've added the comment only to the full-length CFD1; Curated complex link worked for me - must have been a glitch. The Cfd1-Nbp35 complex acts as a scaffold for iron-sulfur protein assembly in the yeast cytosol. The Cfd1-Nbp35 complex acts as a scaffold for iron-sulfur protein assembly in the yeast cytosol. Nature chemical biology 2007 imex curation Netz DJ., Pierik AJ., Stümpfig M., Mühlenhoff U., Lill R. lill@staff.uni-marburg.de 22-Jun-2016: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro uv-vis ultraviolet-visible spectroscopy uv-vis predetermined predetermined participant predetermined Netz DJ., Pierik AJ., Stümpfig M., Mühlenhoff U., Lill R. lill@staff.uni-marburg.de Accepted 2016-MAY-27 AT 08:27 BST AT 08:27 BST by MGT Nature chemical biology 2007 imex curation EBI-11665876 is lane 6 of 1C. All else done.; I found a +1 charge - it was a child of the one I'd picked - but I've only used it for the CFD1 entry where the statement is made. Otherwise I've played safe and left it as the parent.; I've added the comment only to the full-length CFD1; Curated complex link worked for me - must have been a glitch. Mock publication and experiment for abstract interactions that are not interaction evidences. Mock publication and experiment for abstract interactions that are not interaction evidences. unspecified method The Cfd1-Nbp35 complex acts as a scaffold for iron-sulfur protein assembly in the yeast cytosol. The Cfd1-Nbp35 complex acts as a scaffold for iron-sulfur protein assembly in the yeast cytosol. Nature chemical biology 2007 imex curation Netz DJ., Pierik AJ., Stümpfig M., Mühlenhoff U., Lill R. lill@staff.uni-marburg.de 22-Jun-2016: Contacted by IntAct-Help. Only protein-protein interactions ecoli Escherichia coli (strain K12) pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Netz DJ., Pierik AJ., Stümpfig M., Mühlenhoff U., Lill R. lill@staff.uni-marburg.de Accepted 2016-MAY-27 AT 08:27 BST AT 08:27 BST by MGT Nature chemical biology 2007 imex curation EBI-11665876 is lane 6 of 1C. All else done.; I found a +1 charge - it was a child of the one I'd picked - but I've only used it for the CFD1 entry where the statement is made. Otherwise I've played safe and left it as the parent.; I've added the comment only to the full-length CFD1; Curated complex link worked for me - must have been a glitch. cfd1_yeast Cytosolic Fe-S cluster assembly factor CFD1 Cytosolic Fe-S cluster-deficient protein 1 Ribosomal export protein 19 CFD1 DRE3 RIX19 YIA3W YIL003W protein protein yeast Saccharomyces cerevisiae Baker's yeast MEEQEIGVPAASLAGIKHIILILSGKGGVGKSSVTTQTALTLCSMGFKVGVLDIDLTGPSLPRMFGLENESIYQGPEGWQPVKVETNSTGSLSVISLGFLLGDRGNSVIWRGPKKTSMIKQFISDVAWGELDYLLIDTPPGTSDEHISIAEELRYSKPDGGIVVTTPQSVATADVKKEINFCKKVDLKILGIIENMSGFVCPHCAECTNIFSSGGGKRLSEQFSVPYLGNVPIDPKFVEMIENQVSSKKTLVEMYRESSLCPIFEEIMKKLRKQDTTTPVVDKHEQPQIESPK 5CDD563B51229D14 tetra-mu3-sulfido-tetrairon tetra-mu(3)-sulfido-tetrairon (IRON/SULFUR CLUSTER) small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) tetra-mu3-sulfido-tetrairon(1+) tetra-mu3-sulfido-tetrairon(1+) [4Fe-4S](1+) [4Fe-4S](1+) cluster [Fe4(mu3-S)4](+) [Fe4S4](+) Fe4S4(+) tetra-mu3-sulfido-tetrairon(1+) small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) nbp35_yeast Cytosolic Fe-S cluster assembly factor NBP35 NBP35 Nucleotide-binding protein 35 YGL091C protein protein yeast Saccharomyces cerevisiae Baker's yeast MTEILPHVNDEVLPAEYELNQPEPEHCPGPESDMAGKSDACGGCANKEICESLPKGPDPDIPLITDNLSGIEHKILVLSGKGGVGKSTFAAMLSWALSADEDLQVGAMDLDICGPSLPHMLGCIKETVHESNSGWTPVYVTDNLATMSIQYMLPEDDSAIIWRGSKKNLLIKKFLKDVDWDKLDYLVIDTPPGTSDEHISINKYMRESGIDGALVVTTPQEVALLDVRKEIDFCKKAGINILGLVENMSGFVCPNCKGESQIFKATTGGGEALCKELGIKFLGSVPLDPRIGKSCDMGESFLDNYPDSPASSAVLNVVEALRDAVGDV 4B2EADFD0555BCAA leuc_yeast 3-isopropylmalate dehydratase YGL009C LEU1 Isopropylmalate isomerase Alpha-IPM isomerase protein protein yeast Saccharomyces cerevisiae Baker's yeast MVYTPSKGPRTLYDKVFDAHVVHQDENGSFLLYIDRHLVHEVTSPQAFEGLENAGRKVRRVDCTLATVDHNIPTESRKNFKSLDTFIKQTDSRLQVKTLENNVKQFGVPYFGMSDARQGIVHTIGPEEGFTLPGTTVVCGDSHTSTHGAFGSLAFGIGTSEVEHVLATQTIIQAKSKNMRITVNGKLSPGITSKDLILYIIGLIGTAGGTGCVIEFAGEAIEALSMEARMSMCNMAIEAGARAGMIKPDETTFQYTKGRPLAPKGAEWEKAVAYWKTLKTDEGAKFDHEINIEAVDVIPTITWGTSPQDALPITGSVPDPKNVTDPIKKSGMERALAYMGLEPNTPLKSIKVDKVFIGSCTNGRIEDLRSAAAVVRGQKLASNIKLAMVVPGSGLVKKQAEAEGLDKIFQEAGFEWREAGCSICLGMNPDILDAYERCASTSNRNFEGRQGALSRTHLMSPAMAAAAGIAGHFVDIREFEYKDQDQSSPKVEVTSEDEKELESAAYDHAEPVQPEDAPQDIANDELKDIPVKSDDTPAKPSSSGMKPFLTLEGISAPLDKANVDTDAIIPKQFLKTIKRTGLKKGLFYEWRFRKDDQGKDQETDFVLNVEPWREAEILVVTGDNFGCGSSREHAPWALKDFGIKSIIAPSYGDIFYNNSFKNGLLPIRLDQQIIIDKLIPIANKGGKLCVDLPNQKILDSDGNVLVDHFEIEPFRKHCLVNGLDDIGITLQKEEYISRYEALRREKYSFLEGGSKLLKFDNVPKRKAVTTTFDKVHQDW BD409A9702AE3E57 cfd1-nbp35-1 3 11 unspecified role unspecified role prey prey c-terminal strep ii tag strep ii tag Strep (II) Strep II Strep (II) Strep II c-term range C-terminal range c-term range c-term range C-terminal range c-term range 8 unspecified role unspecified role bait bait c-terminal tap tagged calmodulin binding peptide plus protein a tag CBP-ProtA tagged CBP-ProtA tagged tap tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range physical association physical association Fig. 1B nbp35-cfd1-1 3 11 unspecified role unspecified role bait bait c-terminal tap tagged calmodulin binding peptide plus protein a tag CBP-ProtA tagged CBP-ProtA tagged tap tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range 8 unspecified role unspecified role prey prey c-terminal strep ii tag strep ii tag Strep (II) Strep II Strep (II) Strep II c-term range C-terminal range c-term range c-term range C-terminal range c-term range physical association physical association Fig. 1B nbp35-cfd1-2 7 11 unspecified role unspecified role bait bait c-terminal strep ii tag strep ii tag Strep (II) Strep II Strep (II) Strep II c-term range C-terminal range c-term range c-term range C-terminal range c-term range 8 unspecified role unspecified role prey prey n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range direct interaction direct interaction Fig. 1C cfd1-nbp35-2 7 11 weight by comassie molecular weight estimation by coomasie staining weight by comassie western blot western blot Immuno blot Immuno blot weight by comassie molecular weight estimation by coomasie staining weight by comassie unspecified role unspecified role prey prey c-terminal strep ii tag strep ii tag Strep (II) Strep II Strep (II) Strep II c-term range C-terminal range c-term range c-term range C-terminal range c-term range 8 weight by comassie molecular weight estimation by coomasie staining weight by comassie western blot western blot Immuno blot Immuno blot weight by comassie molecular weight estimation by coomasie staining weight by comassie unspecified role unspecified role bait bait n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range direct interaction direct interaction Fig. 1C cfd1-nbp35-3 5 8 unspecified role unspecified role neutral component neutral component n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) 10 unspecified role unspecified role neutral component neutral component direct interaction direct interaction Fig. 2 cfd1-nbp35-4 5 11 unspecified role unspecified role neutral component neutral component n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) 8 unspecified role unspecified role neutral component neutral component n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) 9 unspecified role unspecified role neutral component neutral component direct interaction direct interaction Fig. 2 nbp35-tetra_mu3_sulfido_tetrairon-1 5 11 unspecified role unspecified role neutral component neutral component n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) 9 unspecified role unspecified role neutral component neutral component direct interaction direct interaction Fig. 2 nbp35-tetra_mu3_sulfido_tetrairon-2 5 11 unspecified role unspecified role neutral component neutral component n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 9 unspecified role unspecified role neutral component neutral component direct interaction direct interaction Fig. 2 cfd1-nbp35-5 1 11 unspecified role unspecified role neutral component neutral component n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) 8 unspecified role unspecified role neutral component neutral component n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) 9 unspecified role unspecified role neutral component neutral component direct interaction direct interaction Fig.3 nbp35-tetra_mu3_sulfido_tetrairon-3 1 11 unspecified role unspecified role neutral component neutral component n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) 9 unspecified role unspecified role neutral component neutral component direct interaction direct interaction Fig. 3 Mutation of a cysteine residue (Cys253Ala) in the C-terminal cysteine-rich segment of Nbp35 abolished [Fe-S] cluster binding at this motif (Supplementary Fig. 2). cfd1-tetra_mu3_sulfido_tetrairon-1 1 8 unspecified role unspecified role neutral component neutral component n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) 9 unspecified role unspecified role neutral component neutral component direct interaction direct interaction Fig. 3 nbp35-tetra_mu3_sulfido_tetrairon-4 1 11 unspecified role unspecified role neutral component neutral component n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 9 unspecified role unspecified role neutral component neutral component direct interaction direct interaction Fig. 3 cfd1-tetra_mu3_sulfido_tetrairon-2 4 12 acceptor acceptor neutral component neutral component ecoli Escherichia coli (strain K12) 8 donor donor neutral component neutral component n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 4[Fe-S] binding region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 9 unspecified role unspecified role neutral component neutral component cfd1 binding region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 77 75 direct interaction direct interaction Fig. 4A, 4E A cluster transfer stoichiometry of approximately 1:1 was evident for the transfer from Cfd1 to apo-Leu1. nbp35-tetra_mu3_sulfido_tetrairon-5 4 12 acceptor acceptor unspecified role unspecified role ecoli Escherichia coli (strain K12) 11 donor donor neutral component neutral component n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 4[Fe-S] binding region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 9 unspecified role unspecified role neutral component neutral component nbp35 binding region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 82 84 direct interaction direct interaction Fig. 4B, 4E A cluster transfer stoichiometry of approximately 0.5:1 was evident for the transfer from Nbp35 to apo-Leu1. cfd1_nbp35_yeast-leu1-1 4 12 acceptor acceptor neutral component neutral component 88 donor donor neutral component neutral component direct interaction direct interaction Fig 4C, 4E A cluster transfer stoichiometry of approximately 0.5:1 was evident for the transfer from Cfd1–Nbp35 to apo-Leu1. cfd1-nbp35-6 2 11 unspecified role unspecified role neutral component neutral component c-terminal strep ii tag strep ii tag Strep (II) Strep II Strep (II) Strep II c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecoli Escherichia coli (strain K12) 8 unspecified role unspecified role neutral component neutral component n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) direct interaction direct interaction Supp. Fig. 1 nbp35-cfd1-3 7 8 unspecified role unspecified role bait bait c-terminal strep ii tag strep ii tag Strep (II) Strep II Strep (II) Strep II c-term range C-terminal range c-term range c-term range C-terminal range c-term range 11 unspecified role unspecified role prey prey direct interaction direct interaction Fig. 1C nbp35-tetra_mu3_sulfido_tetrairon-6 5 11 unspecified role unspecified role neutral component neutral component n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range P52920:p.Cys253Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain C A Wed May 02 14:56:08 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'cys253ala' Tue Jun 30 18:06:20 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Cys253Ala' ecoli Escherichia coli (strain K12) 9 unspecified role unspecified role neutral component neutral component direct interaction direct interaction Supp Fig. 2 cfd1-nbp35_yeast Nbp35-Cfd1 complex CFD1-NBP35 complex CFD1:NBP35 Ndor1-anamorsin complex NADPH-dependent diflavin oxidoreductase 1-anamorsin complex 11 unspecified role unspecified role cfd1 binding region binding region binding-associated region binding region n-term range n-terminal range n-term range n-term range n-terminal range n-term range undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 4[Fe-S] binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain 4[Fe-S] binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain dimerization region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 9 unspecified role unspecified role cfd1-nbp35 binding region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 8 unspecified role unspecified role nbp35 binding region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 4[Fe-S] binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain 9 unspecified role unspecified role nbp35 binding region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 9 unspecified role unspecified role cfd1-nbp35 binding region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 11 unspecified role unspecified role cfd1 binding region binding region binding-associated region binding region n-term range n-terminal range n-term range n-term range n-terminal range n-term range undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 4[Fe-S] binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain 4[Fe-S] binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain dimerization region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 8 unspecified role unspecified role nbp35 binding region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 4[Fe-S] binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain 9 unspecified role unspecified role nbp35 binding region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 111 104 105 114 106 109 109 112 107 121 124 116 120 116 118 123 119 126 direct interaction direct interaction yeast Saccharomyces cerevisiae Baker's yeast stable complex stable complex physical interaction evidence used in manual assertion physical interaction evidence used in manual assertion IPI inferred from physical interaction Scaffold complex that assembles nascent FeS clusters as part of the iron-sulfur cluster (ISC) assembly system, for transfer to FeS-requiring enzymes. NBP35 appears to bind the major portion of FeS clusters in the complex whereas CFD1 enhances this binding and drives the release of FeS to target apo-FeS proteins. The complex appears to have low level ATPase activity. Molecular weight =140 kDa. CFD1 and NBP35 bind a bridging [4Fe-4S] cluster at their C-termini and the lability of binding of this cluster may be ideal for its transfer from the scaffold complex toward apoproteins. Depending on isolation conditions, there is also evidence of the complex existing as a heterodimer. Heterotetramer Accepted 2016-APR-08 by BMELDAL