UniProt UniProt http://www.uniprot.org Rabenosyn-5 and EHD1 interact and sequentially regulate protein recycling to the plasma membrane. Rabenosyn-5 and EHD1 interact and sequentially regulate protein recycling to the plasma membrane. Molecular biology of the cell 2004 imex curation Naslavsky N., Boehm M., Backlund PS., Caplan S. scaplan@unmc.edu 15-Sep-2016: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro pull down pull down weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Naslavsky N., Boehm M., Backlund PS., Caplan S. scaplan@unmc.edu Accepted 2016-AUG-23 AT 14:23 BST AT 14:23 BST by MGT Molecular biology of the cell 2004 imex curation Rabenosyn-5 and EHD1 interact and sequentially regulate protein recycling to the plasma membrane. Rabenosyn-5 and EHD1 interact and sequentially regulate protein recycling to the plasma membrane. Molecular biology of the cell 2004 imex curation Naslavsky N., Boehm M., Backlund PS., Caplan S. scaplan@unmc.edu 15-Sep-2016: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Naslavsky N., Boehm M., Backlund PS., Caplan S. scaplan@unmc.edu Accepted 2016-AUG-23 AT 14:23 BST AT 14:23 BST by MGT Molecular biology of the cell 2004 imex curation Rabenosyn-5 and EHD1 interact and sequentially regulate protein recycling to the plasma membrane. Rabenosyn-5 and EHD1 interact and sequentially regulate protein recycling to the plasma membrane. Molecular biology of the cell 2004 imex curation Naslavsky N., Boehm M., Backlund PS., Caplan S. scaplan@unmc.edu 15-Sep-2016: Contacted by IntAct-Help. Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Naslavsky N., Boehm M., Backlund PS., Caplan S. scaplan@unmc.edu Accepted 2016-AUG-23 AT 14:23 BST AT 14:23 BST by MGT Molecular biology of the cell 2004 imex curation Rabenosyn-5 and EHD1 interact and sequentially regulate protein recycling to the plasma membrane. Rabenosyn-5 and EHD1 interact and sequentially regulate protein recycling to the plasma membrane. Molecular biology of the cell 2004 imex curation Naslavsky N., Boehm M., Backlund PS., Caplan S. scaplan@unmc.edu 15-Sep-2016: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro pull down pull down sequence tag sequence tag identification sequence tag Naslavsky N., Boehm M., Backlund PS., Caplan S. scaplan@unmc.edu Accepted 2016-AUG-23 AT 14:23 BST AT 14:23 BST by MGT Molecular biology of the cell 2004 imex curation Rabenosyn-5 and EHD1 interact and sequentially regulate protein recycling to the plasma membrane. Rabenosyn-5 and EHD1 interact and sequentially regulate protein recycling to the plasma membrane. Molecular biology of the cell 2004 imex curation Naslavsky N., Boehm M., Backlund PS., Caplan S. scaplan@unmc.edu 15-Sep-2016: Contacted by IntAct-Help. Only protein-protein interactions human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 confocal microscopy confocal microscopy immunostaining immunostaining Naslavsky N., Boehm M., Backlund PS., Caplan S. scaplan@unmc.edu Accepted 2016-AUG-23 AT 14:23 BST AT 14:23 BST by MGT Molecular biology of the cell 2004 imex curation ehd1_human EH domain-containing protein 1 Testilin CDABP0131 EHD1 PAST PAST1 PAST homolog 1 protein protein human Homo sapiens Human MFSWVSKDARRKKEPELFQTVAEGLRQLYAQKLLPLEEHYRFHEFHSPALEDADFDNKPMVLLVGQYSTGKTTFIRHLIEQDFPGMRIGPEPTTDSFIAVMHGPTEGVVPGNALVVDPRRPFRKLNAFGNAFLNRFMCAQLPNPVLDSISIIDTPGILSGEKQRISRGYDFAAVLEWFAERVDRIILLFDAHKLDISDEFSEVIKALKNHEDKIRVVLNKADQIETQQLMRVYGALMWSLGKIINTPEVVRVYIGSFWSHPLLIPDNRKLFEAEEQDLFKDIQSLPRNAALRKLNDLIKRARLAKVHAYIISSLKKEMPNVFGKESKKKELVNNLGEIYQKIEREHQISPGDFPSLRKMQELLQTQDFSKFQALKPKLLDTVDDMLANDIARLMVMVRQEESLMPSQVVKGGAFDGTMNGPFGHGYGEGAGEGIDDVEWVVGKDKPTYDEIFYTLSPVNGKITGANAKKEMVKSKLPNTVLGKIWKLADVDKDGLLDDEEFALANHLIKVKLEGHELPADLPPHLVPPSKRRHE 3330218772A26CE4 ehd2_human EH domain-containing protein 2 EHD2 PAST2 PAST homolog 2 protein protein human Homo sapiens Human MFSWLKRGGARGQQPEAIRTVTSALKELYRTKLLPLEEHYRFGAFHSPALEDADFDGKPMVLVAGQYSTGKTSFIQYLLEQEVPGSRVGPEPTTDCFVAVMHGDTEGTVPGNALVVDPDKPFRKLNPFGNTFLNRFMCAQLPNQVLESISIIDTPGILSGAKQRVSRGYDFPAVLRWFAERVDLIILLFDAHKLEISDEFSEAIGALRGHEDKIRVVLNKADMVETQQLMRVYGALMWALGKVVGTPEVLRVYIGSFWSQPLLVPDNRRLFELEEQDLFRDIQGLPRHAALRKLNDLVKRARLVRVHAYIISYLKKEMPSVFGKENKKKQLILKLPVIFAKIQLEHHISPGDFPDCQKMQELLMAHDFTKFHSLKPKLLEALDEMLTHDIAKLMPLLRQEELESTEVGVQGGAFEGTHMGPFVERGPDEAMEDGEEGSDDEAEWVVTKDKSKYDEIFYNLAPADGKLSGSKAKTWMVGTKLPNSVLGRIWKLSDVDRDGMLDDEEFALASHLIEAKLEGHGLPANLPRRLVPPSKRRHKGSAE 0EBF691B0A707359 ehd3_human EH domain-containing protein 3 EHD3 EHD2 PAST3 PAST homolog 3 protein protein human Homo sapiens Human MFSWLGTDDRRRKDPEVFQTVSEGLKKLYKSKLLPLEEHYRFHEFHSPALEDADFDNKPMVLLVGQYSTGKTTFIRYLLEQDFPGMRIGPEPTTDSFIAVMQGDMEGIIPGNALVVDPKKPFRKLNAFGNAFLNRFVCAQLPNPVLESISVIDTPGILSGEKQRISRGYDFAAVLEWFAERVDRIILLFDAHKLDISDEFSEVIKALKNHEDKMRVVLNKADQIETQQLMRVYGALMWSLGKIVNTPEVIRVYIGSFWSHPLLIPDNRKLFEAEEQDLFRDIQSLPRNAALRKLNDLIKRARLAKVHAYIISSLKKEMPSVFGKDNKKKELVNNLAEIYGRIEREHQISPGDFPNLKRMQDQLQAQDFSKFQPLKSKLLEVVDDMLAHDIAQLMVLVRQEESQRPIQMVKGGAFEGTLHGPFGHGYGEGAGEGIDDAEWVVARDKPMYDEIFYTLSPVDGKITGANAKKEMVRSKLPNSVLGKIWKLADIDKDGMLDDDEFALANHLIKVKLEGHELPNELPAHLLPPSKRKVAE 0F8AE99F84B966AD rbns5_human Rabenosyn-5 RBSN 110 kDa protein FYVE finger-containing Rab5 effector protein rabenosyn-5 RAB effector RBSN ZFYVE20 Zinc finger FYVE domain-containing protein 20 protein protein human Homo sapiens Human MASLDDPGEVREGFLCPLCLKDLQSFYQLHSHYEEEHSGEDRDVKGQIKSLVQKAKKAKDRLLKREGDDRAESGTQGYESFSYGGVDPYMWEPQELGAVRSHLSDFKKHRAARIDHYVVEVNKLIIRLEKLTAFDRTNTESAKIRAIEKSVVPWVNDQDVPFCPDCGNKFSIRNRRHHCRLCGSIMCKKCMELISLPLANKLTSASKESLSTHTSPSQSPNSVHGSRRGSISSMSSVSSVLDEKDDDRIRCCTHCKDTLLKREQQIDEKEHTPDIVKLYEKLRLCMEKVDQKAPEYIRMAASLNAGETTYSLEHASDLRVEVQKVYELIDALSKKILTLGLNQDPPPHPSNLRLQRMIRYSATLFVQEKLLGLMSLPTKEQFEELKKKRKEEMERKRAVERQAALESQRRLEERQSGLASRAANGEVASLRRGPAPLRKAEGWLPLSGGQGQSEDSDPLLQQIHNITSFIRQAKAAGRMDEVRTLQENLRQLQDEYDQQQTEKAIELSRRQAEEEDLQREQLQMLRERELEREREQFRVASLHTRTRSLDFREIGPFQLEPSREPRTHLAYALDLGSSPVPSSTAPKTPSLSSTQPTRVWSGPPAVGQERLPQSSMPQQHEGPSLNPFDEEDLSSPMEEATTGPPAAGVSLDPSARILKEYNPFEEEDEEEEAVAGNPFIQPDSPAPNPFSEEDEHPQQRLSSPLVPGNPFEEPTCINPFEMDSDSGPEAEEPIEEELLLQQIDNIKAYIFDAKQCGRLDEVEVLTENLRELKHTLAKQKGGTD D49E0E5A95B18616 e1b9v6_bovin RBSN protein protein bovin Bos taurus (Bovine) Bovine MASLDDPGEVREGFLCPLCLKDLQSFYQLQSHYEEEHSGEDRDVKGQIKSLVQKARKAKNRLLKREGDDRAESGTQGYESFSYGGVDPYMWEPQELGAMRSHLSDFKKHRAARIDHYVVEVNKLIIRLEKLTAFDRTNTESAKIRAIEKSVVPWVNDQDVPFCPDCGNKFSIRNRRHHCRLCGSIMCKKCMELISLPLANKLTSASKDSLSAHTSPSQSPNSVHGSRRGSISSVSSVSSVLDEKDDDRIRCCTHCKDTLLKREQQIDEKERTPDIVRLYEKLRLCMEKVDQKAPEYIKMAASLNAGETTYSLEHAGDLRVEVQKVYELIDALSKKILTLDMKQDPPPHPNTLRLQRMIRYSATLFVQEKLLGLMSLPTKEQFEELKQKRKQEMEQKRILERQAALESQRRLEERRSDLASRATNGEVAPPRRGPAPLRKAEGWLPLSEGQRQSEDPDPLLQQIHNITSFIRQAKAAGRTDEVRTLQENLRQLQDEYDQQQTEKAIALSRRQAEEEDLQREQLQVLREREREREQGQAASLHTRTRSLDFREIRPFQLEPSREPRTHLAHALDLGSSPARSGTATKTSPPSSAPEPFRGWSGPPALGQEFLPQSTASPRSEAPSLNPFDEEEDLASPSAEDTANPPAPEPSLGPPACVLREYNPFEEEDEEDAVAGNPFTKPDGPAPNPFEEEDEHPPRTPASPPVPGNPFEEAPCTNPFEEESDGGQEGAEPIEEELLLQQIDNIKAYIFDAKQCGRLDEVEVLTENLRELRRTLARQKGGTD B23FA595D306FD2E ehd1-rbsn-1 4 10 unspecified role unspecified role prey prey cell lysate cell lysate bovin-brain Bos taurus brain brain Brain [cerebrum] 6 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) physical association physical association 1B ehd1-rbsn-2 2 10 unspecified role unspecified role prey prey cell lysate cell lysate bovin-brain Bos taurus brain brain Brain [cerebrum] 6 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) physical association physical association 1C ehd1-rbsn-3 1 9 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain Q9H1K0:p.[Asn626Ala;Phe628Ala;Asn662Ala;Phe664Ala] mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain F A certain certain sequence position certain certain certain sequence position certain N A certain certain sequence position certain certain certain sequence position certain N A certain certain sequence position certain certain certain sequence position certain F A Wed May 02 14:57:56 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asn626ala,phe628ala,asn662ala,phe664ala' Tue Jun 30 18:16:02 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Asn626Ala;Phe628Ala;Asn662Ala;Phe664Ala]' Q9H1K0:p.[Asn626Ala;Phe628Ala] mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain N A certain certain sequence position certain certain certain sequence position certain F A Wed May 02 19:12:40 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asn626ala,phe628ala' Tue Jun 30 17:10:41 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Asn626Ala;Phe628Ala]' Q9H1K0:p.[Asn662Ala;Phe664Ala] mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain N A certain certain sequence position certain certain certain sequence position certain F A Wed May 02 18:48:43 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asn662ala,phe664ala' Tue Jun 30 18:24:10 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Asn662Ala;Phe664Ala]' in vitro In vitro 6 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) physical association physical association 1D and 3 ehd1-rbsn-4 3 9 unspecified role unspecified role prey prey 6 unspecified role unspecified role bait bait eh_domain sufficient to bind sufficient binding region sufficient to bind range range certain certain sequence position certain Q9H4M9:p.Trp485Ala mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain W A Wed May 02 15:43:07 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'trp485ala' Tue Jun 30 18:56:35 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Trp485Ala' physical association physical association 2 ehd2-rbsn-1 3 7 unspecified role unspecified role bait bait 9 unspecified role unspecified role prey prey physical association physical association 2 ehd3-rbsn-1 3 8 unspecified role unspecified role bait bait 9 unspecified role unspecified role prey prey physical association physical association 2 ehd1-rbsn-5 5 9 unspecified role unspecified role neutral component neutral component 6 anti tag immunost anti tag immunostaining anti tag immunost immunostaining immunostaining unspecified role unspecified role neutral component neutral component nucl transfection nucleic acid transfection nucl transfection region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined colocalization colocalization 4C