UniProt UniProt http://www.uniprot.org The farnesoid X receptor controls gene expression in a ligand- and promoter-selective fashion. The farnesoid X receptor controls gene expression in a ligand- and promoter-selective fashion. The Journal of biological chemistry 2004 imex curation Lew JL., Zhao A., Yu J., Huang L., De Pedro N., Peláez F., Wright SD., Cui J., Lew JL., Zhao A., Yu J., Huang L., De Pedro N., Peláez F., Wright SD., Cui J. IBD - Inflammatory bowel disease Origin and expression of NCOA1/SRC-1 and HTRF set-up according PMID 9773982 jisong_cui@merck.com 22-Feb-2016: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro htrf homogeneous time resolved fluorescence homogeneous time-resolved fluorescence htrf tag fluorescence tag visualisation by fluorescence tag fluorescence Lew JL., Zhao A., Yu J., Huang L., De Pedro N., Peláez F., Wright SD., Cui J., Lew JL., Zhao A., Yu J., Huang L., De Pedro N., Peláez F., Wright SD., Cui J. IBD - Inflammatory bowel disease Origin and expression of NCOA1/SRC-1 and HTRF set-up according PMID 9773982 jisong_cui@merck.com Accepted 2016-FEB-05 AT 08:54 GMT AT 08:54 GMT by MGT The Journal of biological chemistry 2004 imex curation OK, just did one more interaction for 1a fxr:src1 without ligand, EBI-11565105 The farnesoid X receptor controls gene expression in a ligand- and promoter-selective fashion. The farnesoid X receptor controls gene expression in a ligand- and promoter-selective fashion. The Journal of biological chemistry 2004 imex curation Lew JL., Zhao A., Yu J., Huang L., De Pedro N., Peláez F., Wright SD., Cui J., Lew JL., Zhao A., Yu J., Huang L., De Pedro N., Peláez F., Wright SD., Cui J. IBD - Inflammatory bowel disease Origin and expression of NCOA1/SRC-1 and HTRF set-up according PMID 9773982 jisong_cui@merck.com 22-Feb-2016: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro pull down pull down weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Lew JL., Zhao A., Yu J., Huang L., De Pedro N., Peláez F., Wright SD., Cui J., Lew JL., Zhao A., Yu J., Huang L., De Pedro N., Peláez F., Wright SD., Cui J. IBD - Inflammatory bowel disease Origin and expression of NCOA1/SRC-1 and HTRF set-up according PMID 9773982 jisong_cui@merck.com Accepted 2016-FEB-05 AT 08:54 GMT AT 08:54 GMT by MGT The Journal of biological chemistry 2004 imex curation OK, just did one more interaction for 1a fxr:src1 without ligand, EBI-11565105 glycochenodeoxycholic acid glycochenodeoxycholic acid chenodeoxycholylglycine Chenodeoxyglycocholate chenodeoxyglycocholic acid GCDCA glycine chenodeoxycholate Glycochenodeoxycholate GLYCOCHENODEOXYCHOLIC ACID Glycochenodeoxycholic acid [(3alpha,7alpha-dihydroxy-24-oxo-5beta-cholan-24-yl)amino]acetic acid N-(3alpha,7alpha-dihydroxy-5beta-cholan-24-oyl)glycine small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) taurochenodeoxycholic acid taurochenodeoxycholic acid Chenodeoxycholoyltaurine taurine chenodeoxycholate taurochenodeoxycholate taurochenodeoxycholic acid Taurochenodeoxycholic acid 2-[(3alpha,7alpha-dihydroxy-24-oxo-5beta-cholan-24-yl)amino]ethanesulfonic acid small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) ncoa1_human Nuclear receptor coactivator 1 Steroid receptor coactivator 1 RIP160 Protein Hin-2 Renal carcinoma antigen NY-REN-52 NCOA1 SRC1 BHLHE74 Class E basic helix-loop-helix protein 74 protein protein human Homo sapiens Human MSGLGDSSSDPANPDSHKRKGSPCDTLASSTEKRRREQENKYLEELAELLSANISDIDSLSVKPDKCKILKKTVDQIQLMKRMEQEKSTTDDDVQKSDISSSSQGVIEKESLGPLLLEALDGFFFVVNCEGRIVFVSENVTSYLGYNQEELMNTSVYSILHVGDHAEFVKNLLPKSLVNGVPWPQEATRRNSHTFNCRMLIHPPDEPGTENQEACQRYEVMQCFTVSQPKSIQEDGEDFQSCLICIARRLPRPPAITGVESFMTKQDTTGKIISIDTSSLRAAGRTGWEDLVRKCIYAFFQPQGREPSYARQLFQEVMTRGTASSPSYRFILNDGTMLSAHTKCKLCYPQSPDMQPFIMGIHIIDREHSGLSPQDDTNSGMSIPRVNPSVNPSISPAHGVARSSTLPPSNSNMVSTRINRQQSSDLHSSSHSNSSNSQGSFGCSPGSQIVANVALNQGQASSQSSNPSLNLNNSPMEGTGISLAQFMSPRRQVTSGLATRPRMPNNSFPPNISTLSSPVGMTSSACNNNNRSYSNIPVTSLQGMNEGPNNSVGFSASSPVLRQMSSQNSPSRLNIQPAKAESKDNKEIASILNEMIQSDNSSSDGKPLDSGLLHNNDRLSDGDSKYSQTSHKLVQLLTTTAEQQLRHADIDTSCKDVLSCTGTSNSASANSSGGSCPSSHSSLTERHKILHRLLQEGSPSDITTLSVEPDKKDSASTSVSVTGQVQGNSSIKLELDASKKKESKDHQLLRYLLDKDEKDLRSTPNLSLDDVKVKVEKKEQMDPCNTNPTPMTKPTPEEIKLEAQSQFTADLDQFDQLLPTLEKAAQLPGLCETDRMDGAVTSVTIKSEILPASLQSATARPTSRLNRLPELELEAIDNQFGQPGTGDQIPWTNNTVTAINQSKSEDQCISSQLDELLCPPTTVEGRNDEKALLEQLVSFLSGKDETELAELDRALGIDKLVQGGGLDVLSERFPPQQATPPLIMEERPNLYSQPYSSPSPTANLPSPFQGMVRQKPSLGTMPVQVTPPRGAFSPGMGMQPRQTLNRPPAAPNQLRLQLQQRLQGQQQLIHQNRQAILNQFAATAPVGINMRSGMQQQITPQPPLNAQMLAQRQRELYSQQHRQRQLIQQQRAMLMRQQSFGNNLPPSSGLPVQMGNPRLPQGAPQQFPYPPNYGTNPGTPPASTSPFSQLAANPEASLANRNSMVSRGMTGNIGGQFGTGINPQMQQNVFQYPGAGMVPQGEANFAPSLSPGSSMVPMPIPPPQSSLLQQTPPASGYQSPDMKAWQQGAIGNNNVFSQAVQNQPTPAQPGVYNNMSITVSMAGGNTNVQNMNPMMAQMQMSSLQMPGMNTVCPEQINDPALRHTGLYCNQLSSTDLLKTEADGTQQVQQVQVFADVQCTVNLVGGDPYLNQPGPLGTQKPTSGPQTPQAQQKSLLQQLLTE DIP protein Q15788-1 original sequence version: 60 DIP protein Q15788-1 original sequence version: 62 DIP protein Q15788 original sequence version: 88 DIP protein Q15788 original sequence version: 107 DIP protein Q15788 original sequence version: 96 25EF6F389489121E chenodeoxycholic acid chenodeoxycholic acid 3alpha,7alpha-Dihydroxy-5beta-cholanic acid 7alpha-hydroxylithocholic acid anthropodeoxycholic acid anthropodesoxycholic acid CDCA chenic acid Chenix Chenodeoxycholic acid Chenodiol gallodesoxycholic acid 3alpha,7alpha-dihydroxy-5beta-cholan-24-oic acid small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) cholate cholate cholate 3alpha,7alpha,12alpha-trihydroxy-5beta-cholan-24-oate 3alpha,7alpha,12alpha-trihydroxy-5beta-cholanate small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) glycocholic acid glycocholic acid 3alpha,7alpha,12alpha-trihydroxy-5beta-cholan-24-oylglycine 3alpha,7alpha,12alpha-Trihydroxy-5beta-cholan-24-oylglycine Glycocholic acid GLYCOCHOLIC ACID N-[(3alpha,5beta,7alpha,12alpha)-3,7,12-trihydroxy-24-oxocholan-24-yl]glycine N-choloylglycine N-(3alpha,7alpha,12alpha-trihydroxy-5beta-cholan-24-oyl)glycine small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) nr1h4_human Bile acid receptor NR1H4 BAR FXR HRR1 RIP14 Farnesoid X-activated receptor Farnesol receptor HRR-1 Nuclear receptor subfamily 1 group H member 4 Retinoid X receptor-interacting protein 14 protein protein human Homo sapiens Human MVMQFQGLENPIQISPHCSCTPSGFFMEMMSMKPAKGVLTEQVAGPLGQNLEVEPYSQYSNVQFPQVQPQISSSSYYSNLGFYPQQPEEWYSPGIYELRRMPAETLYQGETEVAEMPVTKKPRMGASAGRIKGDELCVVCGDRASGYHYNALTCEGCKGFFRRSITKNAVYKCKNGGNCVMDMYMRRKCQECRLRKCKEMGMLAECMYTGLLTEIQCKSKRLRKNVKQHADQTVNEDSEGRDLRQVTSTTKSCREKTELTPDQQTLLHFIMDSYNKQRMPQEITNKILKEEFSAEENFLILTEMATNHVQVLVEFTKKLPGFQTLDHEDQIALLKGSAVEAMFLRSAEIFNKKLPSGHSDLLEERIRNSGISDEYITPMFSFYKSIGELKMTQEEYALLTAIVILSPDRQYIKDREAVEKLQEPLLDVLQKLCKIHQPENPQHFACLLGRLTELRTFNHHHAEMLMSWRVNDHKFTPLLCEIWDVQ C23283576A8CF76B taurocholic acid taurocholic acid 3alpha,7alpha,12alpha-trihydroxy-5beta-cholanic acid 24-taurine cholic acid taurine conjugate Choloyl-taurine Cholyltaurine N-choloyltaurine Taurocholate Taurocholic acid 2-[(3alpha,7alpha,12alpha-trihydroxy-24-oxo-5beta-cholan-24-yl)amino]ethanesulfonic acid small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) WBWWGRHZICKQGZ-HZAMXZRMSA-N WBWWGRHZICKQGZ-HZAMXZRMSA-N ncoa1-nr1h4-4 1 6 stimulator stimulator unspecified role unspecified role 5 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 9 unspecified role unspecified role fluorescence donor fluorescence donor region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined nucl_hrmn_rcpt_lig-bd sufficient to bind sufficient binding region sufficient to bind range range range range ecoli Escherichia coli (strain K12) direct interaction direct interaction 1 A/B CHEBI:16755 increases the binding of Q96RI1 to Q15788 CHEBI:16755 increases the expression at protein level of Q96RI1 ncoa1-nr1h4-5 1 7 stimulator stimulator unspecified role unspecified role 5 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 9 unspecified role unspecified role fluorescence donor fluorescence donor region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined nucl_hrmn_rcpt_lig-bd sufficient to bind sufficient binding region sufficient to bind range range range range ecoli Escherichia coli (strain K12) direct interaction direct interaction 1 A/B CHEBI:29747 increases the binding of Q96RI1 to Q15788 CHEBI:29747 increases the expression at protein level of Q96RI1 ncoa1-nr1h4-6 1 4 stimulator stimulator unspecified role unspecified role 5 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 9 unspecified role unspecified role fluorescence donor fluorescence donor region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined nucl_hrmn_rcpt_lig-bd sufficient to bind sufficient binding region sufficient to bind range range range range ecoli Escherichia coli (strain K12) direct interaction direct interaction 1B CHEBI:16525 increases the binding of Q96RI1 to Q15788 ncoa1-nr1h4-7 1 9 unspecified role unspecified role fluorescence donor fluorescence donor region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined nucl_hrmn_rcpt_lig-bd sufficient to bind sufficient binding region sufficient to bind range range range range ecoli Escherichia coli (strain K12) 3 stimulator stimulator unspecified role unspecified role 5 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) direct interaction direct interaction 1B CHEBI:36274 increases the binding of Q96RI1 to Q15788 ncoa1-nr1h4-8 1 8 stimulator stimulator unspecified role unspecified role 5 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 9 unspecified role unspecified role fluorescence donor fluorescence donor region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined nucl_hrmn_rcpt_lig-bd sufficient to bind sufficient binding region sufficient to bind range range range range ecoli Escherichia coli (strain K12) direct interaction direct interaction 1B CHEBI:17687 increases the binding of Q96RI1 to Q15788 ncoa1-nr1h4-9 1 10 stimulator stimulator unspecified role unspecified role 5 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 9 unspecified role unspecified role fluorescence donor fluorescence donor region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined nucl_hrmn_rcpt_lig-bd sufficient to bind sufficient binding region sufficient to bind range range range range ecoli Escherichia coli (strain K12) direct interaction direct interaction 1B CHEBI:28865 increases the binding of Q96RI1 to Q15788 ncoa1-nr1h4-10 2 5 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 9 unspecified role unspecified role prey prey nucl_hrmn_rcpt_lig-bd sufficient to bind sufficient binding region sufficient to bind range range range range region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro direct interaction direct interaction 2 chenodeoxycholic acid (CHEBI:16755) GW4064 (a synthetic FXR agonist) also promoted interaction nr1h4-ncoa1-1 1 5 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 9 unspecified role unspecified role fluorescence donor fluorescence donor region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined nucl_hrmn_rcpt_lig-bd sufficient to bind sufficient binding region sufficient to bind range range range range ecoli Escherichia coli (strain K12) direct interaction direct interaction 1 A