UniProt UniProt http://www.uniprot.org Structural basis for bile acid binding and activation of the nuclear receptor FXR. Structural basis for bile acid binding and activation of the nuclear receptor FXR. Molecular cell 2003 imex curation Mi LZ., Devarakonda S., Harp JM., Han Q., Pellicciari R., Willson TM., Khorasanizadeh S., Rastinejad F. IBD - Inflammatory bowel disease Sequences of the short synthetic NCOA2/GRIP-1 peptides used are identical for human, mouse and rat. However only mouse UniProt entry lists GRIP-1 as synonym and has been chosen. fr9c@virginia.edu 22-Feb-2016: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro fps fluorescence polarization spectroscopy Fluorescence anisotropy FPS FPS Fluorescence anisotropy fps tag fluorescence tag visualisation by fluorescence tag fluorescence Mi LZ., Devarakonda S., Harp JM., Han Q., Pellicciari R., Willson TM., Khorasanizadeh S., Rastinejad F. IBD - Inflammatory bowel disease Sequences of the short synthetic NCOA2/GRIP-1 peptides used are identical for human, mouse and rat. However only mouse UniProt entry lists GRIP-1 as synonym and has been chosen. fr9c@virginia.edu Accepted 2016-FEB-04 AT 08:42 GMT AT 08:42 GMT by MGT Molecular cell 2003 imex curation Structural basis for bile acid binding and activation of the nuclear receptor FXR. Structural basis for bile acid binding and activation of the nuclear receptor FXR. Molecular cell 2003 imex curation Mi LZ., Devarakonda S., Harp JM., Han Q., Pellicciari R., Willson TM., Khorasanizadeh S., Rastinejad F. IBD - Inflammatory bowel disease Sequences of the short synthetic NCOA2/GRIP-1 peptides used are identical for human, mouse and rat. However only mouse UniProt entry lists GRIP-1 as synonym and has been chosen. fr9c@virginia.edu 22-Feb-2016: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Mi LZ., Devarakonda S., Harp JM., Han Q., Pellicciari R., Willson TM., Khorasanizadeh S., Rastinejad F. IBD - Inflammatory bowel disease Sequences of the short synthetic NCOA2/GRIP-1 peptides used are identical for human, mouse and rat. However only mouse UniProt entry lists GRIP-1 as synonym and has been chosen. fr9c@virginia.edu Accepted 2016-FEB-04 AT 08:42 GMT AT 08:42 GMT by MGT Molecular cell 2003 imex curation Structural basis for bile acid binding and activation of the nuclear receptor FXR. Structural basis for bile acid binding and activation of the nuclear receptor FXR. Molecular cell 2003 imex curation Mi LZ., Devarakonda S., Harp JM., Han Q., Pellicciari R., Willson TM., Khorasanizadeh S., Rastinejad F. IBD - Inflammatory bowel disease Sequences of the short synthetic NCOA2/GRIP-1 peptides used are identical for human, mouse and rat. However only mouse UniProt entry lists GRIP-1 as synonym and has been chosen. fr9c@virginia.edu 22-Feb-2016: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Mi LZ., Devarakonda S., Harp JM., Han Q., Pellicciari R., Willson TM., Khorasanizadeh S., Rastinejad F. IBD - Inflammatory bowel disease Sequences of the short synthetic NCOA2/GRIP-1 peptides used are identical for human, mouse and rat. However only mouse UniProt entry lists GRIP-1 as synonym and has been chosen. fr9c@virginia.edu Accepted 2016-FEB-04 AT 08:42 GMT AT 08:42 GMT by MGT Molecular cell 2003 imex curation nr1h4_rat Bile acid receptor Farnesoid X-activated receptor Farnesol receptor HRR-1 Nuclear receptor subfamily 1 group H member 4 Retinoid X receptor-interacting protein 14 Nr1h4 Bar Fxr Rip14 protein protein rat Rattus norvegicus (Rat) Rat MNLIGPSHLQATDEFALSENLFGVLTEHAAGPLGQNLDLESYSPYNNVQFPQVQPQISSSSYYSNLGFYPQQPEDWYSPGLYELRRMPTESVYQGETEVSEMPVTKKPRMAASSAGRIKGDELCVVCGDRASGYHYNALTCEGCKGFFRRSITKNAVYKCKNGGNCVMDMYMRRKCQDCRLRKCREMGMLAECLLTEIQCKSKRLRKNVKQHADQTVNEDSEGRDLRQVTSTTKLCREKTELTVDQQTLLDYIMDSYSKQRMPQEITNKILKEEFSAEENFLILTEMATSHVQILVEFTKRLPGFQTLDHEDQIALLKGSAVEAMFLRSAEIFNKKLPAGHADLLEERIRKSGISDEYITPMFSFYKSVGELKMTQEEYALLTAIVILSPDRQYIKDREAVEKLQEPLLDVLQKLCKIYQPENPQHFACLLGRLTELRTFNHHHAEMLMSWRVNDHKFTPLLCEIWDVQ 2BEAF5847D9E403C 3-deoxychenodeoxycholic acid 3-deoxychenodeoxycholic acid 3-deoxy-CDCA 3-deoxyCDCA 7alpha-hydroxy-5beta-cholan-24-oic acid 7alpha-hydroxy-5beta-cholanic acid (5beta,7alpha)-7-hydroxycholan-24-oic acid small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) chenodeoxycholic acid chenodeoxycholic acid 3alpha,7alpha-Dihydroxy-5beta-cholanic acid 7alpha-hydroxylithocholic acid anthropodeoxycholic acid anthropodesoxycholic acid CDCA chenic acid Chenix Chenodeoxycholic acid Chenodiol gallodesoxycholic acid 3alpha,7alpha-dihydroxy-5beta-cholan-24-oic acid small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) 6-ethyl-chenodeoxycholic acid 6-ETHYL-CHENODEOXYCHOLIC ACID small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) ncoa2_mouse Nuclear receptor coactivator 2 Transcriptional intermediary factor 2 Glucocorticoid receptor-interacting protein 1 Ncoa2 Grip1 Tif2 Src2 Steroid receptor coactivator 2 protein protein mouse Mus musculus Mouse MSGMGENTSDPSRAETRKRKECPDQLGPSPKRSTEKRNREQENKYIEELAELIFANFNDIDNFNFKPDKCAILKETVKQIRQIKEQEKAAAANIDEVQKSDVSSTGQGVIDKDALGPMMLEALDGFFFVVNLEGSVVFVSENVTQYLRYNQEELMNKSVYSILHVGDHTEFVKNLLPKSMVNGGSWSGEPPRRSSHTFNCRMLVKPLPDSEEEGHDSQEAHQKYEAMQCFAVSQPKSIKEEGEDLQSCLICVARRVPMKERPTLPSSESFTTRQDLQGKITSLDTSTMRAAMKPGWEDLVRRCIQKFHTQHEGESLSYAKRHHHEVLRQGLAFSQIYRFSLSDGTLVAAQTKSKLIRSQTTNEPQLVISLHMLHREQNVCVMNPDLTGQAMGKPLNPISSSSPAHQALCSGNPGQDMTLGSNINFPMNGPKEQMGMPMGRFGGSGGMNHVSGMQATTPQGSNYALKMNSPSQSSPGMNPGQASSVLSPRQRMSPGVAGSPRIPPSQFSPAGSLHSPVGVCSSTGNSHSYTNSSLNALQALSEGHGVSLGSSLASPDLKMGNLQNSPVNMNPPPLSKMGSLDSKDCFGLYGEPSEGTTGQAEASCHPEEQKGPNDSSMPQAASGDRAEGHSRLHDSKGQTKLLQLLTTKSDQMEPSPLPSSLSDTNKDSTGSLPGPGSTHGTSLKEKHKILHRLLQDSSSPVDLAKLTAEATGKELSQESSSTAPGSEVTVKQEPASPKKKENALLRYLLDKDDTKDIGLPEITPKLERLDSKTDPASNTKLIAMKTVKEEVSFEPSDQPGSELDNLEEILDDLQNSQLPQLFPDTRPGAPTGSVDKQAIINDLMQLTADSSPVPPAGAQKAALRMSQSTFNNPRPGQLGRLLPNQNLPLDITLQSPTGAGPFPPIRNSSPYSVIPQPGMMGNQGMLGSQGNLGNNSTGMIGSSTSRPSMPSGEWAPQSPAVRVTCAATTGAMNRPVQGGMIRNPTASIPMRANSQPGQRQMLQSQVMNIGPSELEMNMGGPQYNQQQAPPNQTAPWPESILPIDQASFASQNRQPFGSSPDDLLCPHPAAESPSDEGALLDQLYLALRNFDGLEEIDRALGIPELVSQSQAVDAEQFSSQESSIMLEQKPPVFPQQYASQAQMAQGGYNPMQDPNFHTMGQRPNYTTLRMQPRPGLRPTGIVQNQPNQLRLQLQHRLQAQQNRQPLMNQISSVSNVNLTLRPGVPTQAPINAQMLAQRQREILNQHLRQRQMQQQVQQRTLMMRGQGLNVTPSMVAPAGLPAAMSNPRIPQANAQQFPFPPNYGISQQPDPGFTGATTPQSPLMSPRMAHTQSPMMQQSQANPAYQPTSDMNGWAQGSMGGNSMFSQQSPPHFGQQANTSMYSNNMNISVSMATNTGGLSSMNQMTGQMSMTSVTSVPTSGLPSMGPEQVNDPALRGGNLFPNQLPGMDMIKQEGDASRKYC DIP protein Q61026 original sequence version: 150 DIP protein Q61026 original sequence version: 105 DIP protein Q61026 original sequence version: 107 619462FF1ACC6067 ncoa2-nr1h4-1 2 7 unspecified role unspecified role neutral component neutral component 8 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 4 unspecified role unspecified role neutral component neutral component nucl_hrmn_rcpt_lig-bd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) direct interaction direct interaction 1, Table 1 2.5 A working: 24,2 %; free: 27,6 % ncoa2-nr1h4-2 2 5 unspecified role unspecified role unspecified role unspecified role 8 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 4 unspecified role unspecified role neutral component neutral component n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range nucl_hrmn_rcpt_lig-bd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) direct interaction direct interaction 1, Table 1 2.9 A working: 25.4 %; free: 29.0 % ncoa2-nr1h4-3 1 6 unspecified role unspecified role unspecified role unspecified role 8 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain n-terminus fluorescein fluorescein label fluorescein n-term range n-terminal range n-term range n-term range n-terminal range n-term range 4 unspecified role unspecified role neutral component neutral component nucl_hrmn_rcpt_lig-bd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) direct interaction direct interaction 3D and 2B ncoa2-nr1h4-4 1 6 unspecified role unspecified role unspecified role unspecified role 8 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain n-terminus fluorescein fluorescein label fluorescein n-term range n-terminal range n-term range n-term range n-terminal range n-term range 4 unspecified role unspecified role neutral component neutral component nucl_hrmn_rcpt_lig-bd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) direct interaction direct interaction 3D ncoa2-nr1h4-5 1 6 unspecified role unspecified role unspecified role unspecified role 8 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain n-terminus fluorescein fluorescein label fluorescein n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) 4 unspecified role unspecified role neutral component neutral component nucl_hrmn_rcpt_lig-bd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) direct interaction direct interaction 3D ncoa2-nr1h4-6 3 8 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 4 unspecified role unspecified role neutral component neutral component nucl_hrmn_rcpt_lig-bd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) direct interaction direct interaction 3E ncoa2-nr1h4-7 1 5 unspecified role unspecified role unspecified role unspecified role 8 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain n-terminus fluorescein fluorescein label fluorescein n-term range n-terminal range n-term range n-term range n-terminal range n-term range 4 unspecified role unspecified role neutral component neutral component nucl_hrmn_rcpt_lig-bd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) direct interaction direct interaction 2B