IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Characterization of a Steroid Receptor Coactivator Small Molecule Stimulator that Overstimulates Cancer Cells and Leads to Cell Stress and Death. Characterization of a Steroid Receptor Coactivator Small Molecule Stimulator that Overstimulates Cancer Cells and Leads to Cell Stress and Death. Cancer cell 2015 imex curation Wang L., Yu Y., Chow DC., Yan F., Hsu CC., Stossi F., Mancini MA., Palzkill T., Liao L., Zhou S., Xu J., Lonard DM., O'Malley BW. GST-Src3 (and Flag-SRC-3) were determined to be of Human origin by following through references to PMID:15383283 and also by mutation mapping in Wu et al., 2007 (PMID:17574025). Mutation mapping indicates SRC3 isoform 1 was used, and Figure 5 of PMID:17574025 gives the "RID" (Receptor Interacting Domain) as amino acids 582-840. However the full length protein is stated to be 1417 amino acids in figure 5;this length does not correspond to any known SRC3 protein. dlonard@bcm.edu,berto@bcm.edu 11-Apr-2016: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro fluorescence spectr classical fluorescence spectroscopy fluorescence spectr predetermined predetermined participant predetermined Wang L., Yu Y., Chow DC., Yan F., Hsu CC., Stossi F., Mancini MA., Palzkill T., Liao L., Zhou S., Xu J., Lonard DM., O'Malley BW. (bug now fixed in PROD) bug reported for variable parameters -display. MArch 2016 - problem with UniProt - cannot deploy new version of ? - bug fixed but not in current version of PROD GST-Src3 (and Flag-SRC-3) were determined to be of Human origin by following through references to PMID:15383283 and also by mutation mapping in Wu et al., 2007 (PMID:17574025). Mutation mapping indicates SRC3 isoform 1 was used, and Figure 5 of PMID:17574025 gives the "RID" (Receptor Interacting Domain) as amino acids 582-840. However the full length protein is stated to be 1417 amino acids in figure 5;this length does not correspond to any known SRC3 protein. dlonard@bcm.edu,berto@bcm.edu Accepted 2016-APR-01 AT 08:49 BST AT 08:49 BST by ORCHARD Cancer cell 2015 Only protein-protein interactions imex curation imex curation altered Characterization of a Steroid Receptor Coactivator Small Molecule Stimulator that Overstimulates Cancer Cells and Leads to Cell Stress and Death. Characterization of a Steroid Receptor Coactivator Small Molecule Stimulator that Overstimulates Cancer Cells and Leads to Cell Stress and Death. Cancer cell 2015 imex curation Wang L., Yu Y., Chow DC., Yan F., Hsu CC., Stossi F., Mancini MA., Palzkill T., Liao L., Zhou S., Xu J., Lonard DM., O'Malley BW. GST-Src3 (and Flag-SRC-3) were determined to be of Human origin by following through references to PMID:15383283 and also by mutation mapping in Wu et al., 2007 (PMID:17574025). Mutation mapping indicates SRC3 isoform 1 was used, and Figure 5 of PMID:17574025 gives the "RID" (Receptor Interacting Domain) as amino acids 582-840. However the full length protein is stated to be 1417 amino acids in figure 5;this length does not correspond to any known SRC3 protein. dlonard@bcm.edu,berto@bcm.edu 11-Apr-2016: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Time in hours 1 4 8 MCB-613 concentration uM 6 8 10 0 Wang L., Yu Y., Chow DC., Yan F., Hsu CC., Stossi F., Mancini MA., Palzkill T., Liao L., Zhou S., Xu J., Lonard DM., O'Malley BW. Results from fig 4E not added as the specificity of the Abl antibody is not known. (bug now fixed in PROD) bug reported for variable parameters -display. MArch 2016 - problem with UniProt - cannot deploy new version of ? - bug fixed but not in current version of PROD GST-Src3 (and Flag-SRC-3) were determined to be of Human origin by following through references to PMID:15383283 and also by mutation mapping in Wu et al., 2007 (PMID:17574025). Mutation mapping indicates SRC3 isoform 1 was used, and Figure 5 of PMID:17574025 gives the "RID" (Receptor Interacting Domain) as amino acids 582-840. However the full length protein is stated to be 1417 amino acids in figure 5;this length does not correspond to any known SRC3 protein. dlonard@bcm.edu,berto@bcm.edu Accepted 2016-APR-01 AT 08:49 BST AT 08:49 BST by ORCHARD Cancer cell 2015 Only protein-protein interactions imex curation imex curation altered Characterization of a Steroid Receptor Coactivator Small Molecule Stimulator that Overstimulates Cancer Cells and Leads to Cell Stress and Death. Characterization of a Steroid Receptor Coactivator Small Molecule Stimulator that Overstimulates Cancer Cells and Leads to Cell Stress and Death. Cancer cell 2015 imex curation Wang L., Yu Y., Chow DC., Yan F., Hsu CC., Stossi F., Mancini MA., Palzkill T., Liao L., Zhou S., Xu J., Lonard DM., O'Malley BW. GST-Src3 (and Flag-SRC-3) were determined to be of Human origin by following through references to PMID:15383283 and also by mutation mapping in Wu et al., 2007 (PMID:17574025). Mutation mapping indicates SRC3 isoform 1 was used, and Figure 5 of PMID:17574025 gives the "RID" (Receptor Interacting Domain) as amino acids 582-840. However the full length protein is stated to be 1417 amino acids in figure 5;this length does not correspond to any known SRC3 protein. dlonard@bcm.edu,berto@bcm.edu 11-Apr-2016: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Wang L., Yu Y., Chow DC., Yan F., Hsu CC., Stossi F., Mancini MA., Palzkill T., Liao L., Zhou S., Xu J., Lonard DM., O'Malley BW. (bug now fixed in PROD) bug reported for variable parameters -display. MArch 2016 - problem with UniProt - cannot deploy new version of ? - bug fixed but not in current version of PROD GST-Src3 (and Flag-SRC-3) were determined to be of Human origin by following through references to PMID:15383283 and also by mutation mapping in Wu et al., 2007 (PMID:17574025). Mutation mapping indicates SRC3 isoform 1 was used, and Figure 5 of PMID:17574025 gives the "RID" (Receptor Interacting Domain) as amino acids 582-840. However the full length protein is stated to be 1417 amino acids in figure 5;this length does not correspond to any known SRC3 protein. dlonard@bcm.edu,berto@bcm.edu Accepted 2016-APR-01 AT 08:49 BST AT 08:49 BST by ORCHARD Cancer cell 2015 Only protein-protein interactions imex curation imex curation altered ncoa3_human Nuclear receptor coactivator 3 NCOA3 AIB1 RAC3 Thyroid hormone receptor activator molecule 1 Receptor-associated coactivator 3 BHLHE42 Steroid receptor coactivator protein 3 CBP-interacting protein Amplified in breast cancer 1 protein ACTR Class E basic helix-loop-helix protein 42 TRAM1 protein protein human Homo sapiens Human MSGLGENLDPLASDSRKRKLPCDTPGQGLTCSGEKRRREQESKYIEELAELISANLSDIDNFNVKPDKCAILKETVRQIRQIKEQGKTISNDDDVQKADVSSTGQGVIDKDSLGPLLLQALDGFLFVVNRDGNIVFVSENVTQYLQYKQEDLVNTSVYNILHEEDRKDFLKNLPKSTVNGVSWTNETQRQKSHTFNCRMLMKTPHDILEDINASPEMRQRYETMQCFALSQPRAMMEEGEDLQSCMICVARRITTGERTFPSNPESFITRHDLSGKVVNIDTNSLRSSMRPGFEDIIRRCIQRFFSLNDGQSWSQKRHYQEAYLNGHAETPVYRFSLADGTIVTAQTKSKLFRNPVTNDRHGFVSTHFLQREQNGYRPNPNPVGQGIRPPMAGCNSSVGGMSMSPNQGLQMPSSRAYGLADPSTTGQMSGARYGGSSNIASLTPGPGMQSPSSYQNNNYGLNMSSPPHGSPGLAPNQQNIMISPRNRGSPKIASHQFSPVAGVHSPMASSGNTGNHSFSSSSLSALQAISEGVGTSLLSTLSSPGPKLDNSPNMNITQPSKVSNQDSKSPLGFYCDQNPVESSMCQSNSRDHLSDKESKESSVEGAENQRGPLESKGHKKLLQLLTCSSDDRGHSSLTNSPLDSSCKESSVSVTSPSGVSSSTSGGVSSTSNMHGSLLQEKHRILHKLLQNGNSPAEVAKITAEATGKDTSSITSCGDGNVVKQEQLSPKKKENNALLRYLLDRDDPSDALSKELQPQVEGVDNKMSQCTSSTIPSSSQEKDPKIKTETSEEGSGDLDNLDAILGDLTSSDFYNNSISSNGSHLGTKQQVFQGTNSLGLKSSQSVQSIRPPYNRAVSLDSPVSVGSSPPVKNISAFPMLPKQPMLGGNPRMMDSQENYGSSMGGPNRNVTVTQTPSSGDWGLPNSKAGRMEPMNSNSMGRPGGDYNTSLPRPALGGSIPTLPLRSNSIPGARPVLQQQQQMLQMRPGEIPMGMGANPYGQAAASNQLGSWPDGMLSMEQVSHGTQNRPLLRNSLDDLVGPPSNLEGQSDERALLDQLHTLLSNTDATGLEEIDRALGIPELVNQGQALEPKQDAFQGQEAAVMMDQKAGLYGQTYPAQGPPMQGGFHLQGQSPSFNSMMNQMNQQGNFPLQGMHPRANIMRPRTNTPKQLRMQLQQRLQGQQFLNQSRQALELKMENPTAGGAAVMRPMMQPQVSSQQGFLNAQMVAQRSRELLSHHFRQQRVAMMMQQQQQQQQQQQQQQQQQQQQQQQQQQQQQTQAFSPPPNVTASPSMDGLLAGPTMPQAPPQQFPYQPNYGMGQQPDPAFGRVSSPPNAMMSSRMGPSQNPMMQHPQAASIYQSSEMKGWPSGNLARNSSFSQQQFAHQGNPAVYSMVHMNGSSGHMGQMNMNPMPMSGMPMGPDQKYC DIP protein Q9Y6Q9 original sequence version: 162 DIP protein Q9Y6Q9 original sequence version: 155 DIP protein Q9Y6Q9 original sequence version: 91 732CDF0423161679 mcb-613 MCB-613 small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) carm1_human Histone-arginine methyltransferase CARM1 CARM1 PRMT4 Protein arginine N-methyltransferase 4 Coactivator-associated arginine methyltransferase 1 protein protein human Homo sapiens Human MAAAAAAVGPGAGGAGSAVPGGAGPCATVSVFPGARLLTIGDANGEIQRHAEQQALRLEVRAGPDSAGIALYSHEDVCVFKCSVSRETECSRVGKQSFIITLGCNSVLIQFATPNDFCSFYNILKTCRGHTLERSVFSERTEESSAVQYFQFYGYLSQQQNMMQDYVRTGTYQRAILQNHTDFKDKIVLDVGCGSGILSFFAAQAGARKIYAVEASTMAQHAEVLVKSNNLTDRIVVIPGKVEEVSLPEQVDIIISEPMGYMLFNERMLESYLHAKKYLKPSGNMFPTIGDVHLAPFTDEQLYMEQFTKANFWYQPSFHGVDLSALRGAAVDEYFRQPVVDTFDIRILMAKSVKYTVNFLEAKEGDLHRIEIPFKFHMLHSGLVHGLAFWFDVAFIGSIMTVWLSTAPTEPLTHWYQVRCLFQSPLFAKAGDTLSGTCLLIANKRQSYDISIVAQVDQTGSKSSNLLDLKNPFFRYTGTTPSPPPGSHYTSPSENMWNTGSTYNLSSGMAVAGMPTAYDLSSVIASGSSVGHNNLIPLANTGIVNHTHSRMGSIMSTGIVQGSSGAQGSGGGSTSAHYAVNSQFTMGGPAISMASPMSIPTNTMHYGS DIP protein Q86X55 original sequence version: 115 094AC60D4A70B263 cbp_human CREB-binding protein CREBBP CBP Histone lysine acetyltransferase CREBBP Protein-lysine acetyltransferase CREBBP protein protein human Homo sapiens Human MAENLLDGPPNPKRAKLSSPGFSANDSTDFGSLFDLENDLPDELIPNGGELGLLNSGNLVPDAASKHKQLSELLRGGSGSSINPGIGNVSASSPVQQGLGGQAQGQPNSANMASLSAMGKSPLSQGDSSAPSLPKQAASTSGPTPAASQALNPQAQKQVGLATSSPATSQTGPGICMNANFNQTHPGLLNSNSGHSLINQASQGQAQVMNGSLGAAGRGRGAGMPYPTPAMQGASSSVLAETLTQVSPQMTGHAGLNTAQAGGMAKMGITGNTSPFGQPFSQAGGQPMGATGVNPQLASKQSMVNSLPTFPTDIKNTSVTNVPNMSQMQTSVGIVPTQAIATGPTADPEKRKLIQQQLVLLLHAHKCQRREQANGEVRACSLPHCRTMKNVLNHMTHCQAGKACQVAHCASSRQIISHWKNCTRHDCPVCLPLKNASDKRNQQTILGSPASGIQNTIGSVGTGQQNATSLSNPNPIDPSSMQRAYAALGLPYMNQPQTQLQPQVPGQQPAQPQTHQQMRTLNPLGNNPMNIPAGGITTDQQPPNLISESALPTSLGATNPLMNDGSNSGNIGTLSTIPTAAPPSSTGVRKGWHEHVTQDLRSHLVHKLVQAIFPTPDPAALKDRRMENLVAYAKKVEGDMYESANSRDEYYHLLAEKIYKIQKELEEKRRSRLHKQGILGNQPALPAPGAQPPVIPQAQPVRPPNGPLSLPVNRMQVSQGMNSFNPMSLGNVQLPQAPMGPRAASPMNHSVQMNSMGSVPGMAISPSRMPQPPNMMGAHTNNMMAQAPAQSQFLPQNQFPSSSGAMSVGMGQPPAQTGVSQGQVPGAALPNPLNMLGPQASQLPCPPVTQSPLHPTPPPASTAAGMPSLQHTTPPGMTPPQPAAPTQPSTPVSSSGQTPTPTPGSVPSATQTQSTPTVQAAAQAQVTPQPQTPVQPPSVATPQSSQQQPTPVHAQPPGTPLSQAAASIDNRVPTPSSVASAETNSQQPGPDVPVLEMKTETQAEDTEPDPGESKGEPRSEMMEEDLQGASQVKEETDIAEQKSEPMEVDEKKPEVKVEVKEEEESSSNGTASQSTSPSQPRKKIFKPEELRQALMPTLEALYRQDPESLPFRQPVDPQLLGIPDYFDIVKNPMDLSTIKRKLDTGQYQEPWQYVDDVWLMFNNAWLYNRKTSRVYKFCSKLAEVFEQEIDPVMQSLGYCCGRKYEFSPQTLCCYGKQLCTIPRDAAYYSYQNRYHFCEKCFTEIQGENVTLGDDPSQPQTTISKDQFEKKKNDTLDPEPFVDCKECGRKMHQICVLHYDIIWPSGFVCDNCLKKTGRPRKENKFSAKRLQTTRLGNHLEDRVNKFLRRQNHPEAGEVFVRVVASSDKTVEVKPGMKSRFVDSGEMSESFPYRTKALFAFEEIDGVDVCFFGMHVQEYGSDCPPPNTRRVYISYLDSIHFFRPRCLRTAVYHEILIGYLEYVKKLGYVTGHIWACPPSEGDDYIFHCHPPDQKIPKPKRLQEWYKKMLDKAFAERIIHDYKDIFKQATEDRLTSAKELPYFEGDFWPNVLEESIKELEQEEEERKKEESTAASETTEGSQGDSKNAKKKNNKKTNKNKSSISRANKKKPSMPNVSNDLSQKLYATMEKHKEVFFVIHLHAGPVINTLPPIVDPDPLLSCDLMDGRDAFLTLARDKHWEFSSLRRSKWSTLCMLVELHTQGQDRFVYTCNECKHHVETRWHCTVCEDYDLCINCYNTKSHAHKMVKWGLGLDDEGSSQGEPQSKSPQESRRLSIQRCIQSLVHACQCRNANCSLPSCQKMKRVVQHTKGCKRKTNGGCPVCKQLIALCCYHAKHCQENKCPVPFCLNIKHKLRQQQIQHRLQQAQLMRRRMATMNTRNVPQQSLPSPTSAPPGTPTQQPSTPQTPQPPAQPQPSPVSMSPAGFPSVARTQPPTTVSTGKPTSQVPAPPPPAQPPPAAVEAARQIEREAQQQQHLYRVNINNSMPPGRTGMGTPGSQMAPVSLNVPRPNQVSGPVMPSMPPGQWQQAPLPQQQPMPGLPRPVISMQAQAAVAGPRMPSVQPPRSISPSALQDLLRTLKSPSSPQQQQQVLNILKSNPQLMAAFIKQRTAKYVANQPGMQPQPGLQSQPGMQPQPGMHQQPSLQNLNAMQAGVPRPGVPPQQQAMGGLNPQGQALNIMNPGHNPNMASMNPQYREMLRRQLLQQQQQQQQQQQQQQQQQQGSAGMAGGMAGHGQFQQPQGPGGYPPAMQQQQRMQQHLPLQGSSMGQMAAQMGQLGQMGQPGLGADSTPNIQQALQQRILQQQQMKQQIGSPGQPNPMSPQQHMLSGQPQASHLPGQQIATSLSNQVRSPAPVQSPRPQSQPPHSSPSPRIQPQPSPHHVSPQTGSPHPGLAVTMASSIDQGHLGNPEQSAMLPQLNTPSRSALSSELSLVGDTTGDTLEKFVEGL DIP protein Q92793 original sequence version: 134 DIP protein Q92793-1 original sequence version: 171 DIP protein Q92793 original sequence version: 171 DIP protein Q92793 original sequence version: 185 DIP protein Q92793 original sequence version: 106 DIP protein Q92793 original sequence version: 104 DIP protein Q92793 original sequence version: 112 DIP protein Q92793 original sequence version: 135 DIP protein Q92793 original sequence version: 139 DIP protein Q92793 original sequence version: 157 DIP protein Q92793 original sequence version: 177 DIP protein Q92793 original sequence version: 168 DIP protein Q92793-1 original sequence version: 174 DIP protein Q92793 original sequence version: 146 DIP protein Q92793 original sequence version: 161 DIP protein Q92793 original sequence version: 172 3BEA9B8558BA1A5E ncoa3-mcb_613-1 10 11 unspecified role unspecified role bait bait rid_region sufficient to bind sufficient binding region sufficient to bind range range range range region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 12 unspecified role unspecified role prey prey direct interaction direct interaction 1G CHEBI:90954 increases the expression at the RNA level of Q9Y6Q9 CHEBI:90954 decreases the expression at the protein level of Q9Y6Q9 CHEBI:28939 decreased the stimulatory effect of CHEBI:90954 on the expression at the RNA level of Q9Y6Q9 CHEBI:28939 reversed the decrease in protein levels of Q9Y6Q9 caused by CHEBI:90954 ncoa3-carm1-1 2 14 unspecified role unspecified role prey prey 13 unspecified role unspecified role prey prey 11 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association 6 4 1H, Supp 1 G/H-1 MCB-613 - CHEBI:90954 ncoa3-carm1-2 2 13 unspecified role unspecified role prey prey 11 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 9 3 1H ncoa3-mcb_613-2 1 11 unspecified role unspecified role neutral component neutral component rid_region sufficient to bind sufficient binding region sufficient to bind range range range range region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 12 unspecified role unspecified role neutral component neutral component direct interaction direct interaction Supp 1F ncoa3-carm1-3 2 14 unspecified role unspecified role prey prey 13 unspecified role unspecified role prey prey 11 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association 5 6 1H, Supp 1 G/H-2 MCB-613 - CHEBI:90954 ncoa3-carm1-4 2 14 unspecified role unspecified role prey prey 13 unspecified role unspecified role prey prey 11 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association 7 3 1H, Supp 1 G/H-3 MCB-613 - CHEBI:90954 ncoa3-carm1-5 2 14 unspecified role unspecified role prey prey 13 unspecified role unspecified role prey prey 11 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association 8 3 1H, Supp 1 G/H-4 MCB-613 - CHEBI:90954 ncoa3-carm1-6 2 14 unspecified role unspecified role prey prey 13 unspecified role unspecified role prey prey 11 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association 6 3 1H, Supp 1 G/H-5 MCB-613 - CHEBI:90954 ncoa3-carm1-7 2 14 unspecified role unspecified role prey prey 13 unspecified role unspecified role prey prey 11 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association 7 4 1H, Supp 1 G/H-6 MCB-613 - CHEBI:90954 ncoa3-carm1-8 2 14 unspecified role unspecified role prey prey 13 unspecified role unspecified role prey prey 11 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association 9 4 1H, Supp 1 G/H-7 MCB-613 - CHEBI:90954 Longer treatment with MCB-613 for 4 or 8 hr increased the SRC-3-CBP interaction, whereas no further interaction between SRC-3 and CARM1 was observed. ncoa3-carm1-9 2 14 unspecified role unspecified role prey prey 13 unspecified role unspecified role prey prey 11 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association 8 4 1H, Supp 1 G/H-8 MCB-613 - CHEBI:90954 Longer treatment with MCB-613 for 4 or 8 hr increased the SRC-3-CBP interaction, whereas no further interaction between SRC-3 and CARM1 was observed. ncoa3-carm1-10 2 14 unspecified role unspecified role prey prey 13 unspecified role unspecified role prey prey 11 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association 9 5 1H, Supp 1 G/H-9 MCB-613 - CHEBI:90954 Longer treatment with MCB-613 for 4 or 8 hr increased the SRC-3-CBP interaction, whereas no further interaction between SRC-3 and CARM1 was observed. ncoa3-carm1-12 2 14 unspecified role unspecified role prey prey 13 unspecified role unspecified role prey prey 11 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association 8 5 1H, Supp 1 G/H-11 MCB-613 - CHEBI:90954 Longer treatment with MCB-613 for 4 or 8 hr increased the SRC-3-CBP interaction, whereas no further interaction between SRC-3 and CARM1 was observed. ncoa3-carm1-13 2 14 unspecified role unspecified role prey prey 13 unspecified role unspecified role prey prey 11 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association 7 5 1H, Supp 1 G/H-10 MCB-613 - CHEBI:90954 Longer treatment with MCB-613 for 4 or 8 hr increased the SRC-3-CBP interaction, whereas no further interaction between SRC-3 and CARM1 was observed.