MINT MINT, Dpt of Biology, University of Rome Tor Vergata MINT http://mint.bio.uniroma2.it/mint url>http://mint.bio.uniroma2.it/mint/ https://mint.bio.uniroma2.it MINT-[0-9]+ https://mint.bio.uniroma2.it/index.php/results-interactions/?id=${ac} The Molecular INTeraction database (MINT) is a relational database designed to store interactions between biological molecules. http://mint.bio.uniroma2.it MMP-2 inhibits PCSK9-induced degradation of the LDL receptor in Hepa1-c1c7 cells. MMP-2 inhibits PCSK9-induced degradation of the LDL receptor in Hepa1-c1c7 cells. FEBS Lett. (0014-5793) 2015 imex curation Wang X., Berry E., Hernandez-Anzaldo S., Sun D., Adijiang A., Li L., Zhang D., Fernandez-Patron C. cf2@ualberta.ca Only protein-protein interactions in vitro In vitro enzymatic study enzymatic study weight by staining molecular weight estimation by staining weight by staining Wang X., Berry E., Hernandez-Anzaldo S., Sun D., Adijiang A., Li L., Zhang D., Fernandez-Patron C. cf2@ualberta.ca Accepted 2015-APR-28 by LUANA FEBS Lett. (0014-5793) 2015 Only protein-protein interactions imex curation MMP-2 inhibits PCSK9-induced degradation of the LDL receptor in Hepa1-c1c7 cells. MMP-2 inhibits PCSK9-induced degradation of the LDL receptor in Hepa1-c1c7 cells. FEBS Lett. (0014-5793) 2015 imex curation Wang X., Berry E., Hernandez-Anzaldo S., Sun D., Adijiang A., Li L., Zhang D., Fernandez-Patron C. cf2@ualberta.ca Only protein-protein interactions in vitro In vitro zymography zymography weight by comassie molecular weight estimation by coomasie staining weight by comassie Wang X., Berry E., Hernandez-Anzaldo S., Sun D., Adijiang A., Li L., Zhang D., Fernandez-Patron C. cf2@ualberta.ca Accepted 2015-APR-28 by LUANA FEBS Lett. (0014-5793) 2015 Only protein-protein interactions imex curation MMP-2 inhibits PCSK9-induced degradation of the LDL receptor in Hepa1-c1c7 cells. MMP-2 inhibits PCSK9-induced degradation of the LDL receptor in Hepa1-c1c7 cells. FEBS Lett. (0014-5793) 2015 imex curation Wang X., Berry E., Hernandez-Anzaldo S., Sun D., Adijiang A., Li L., Zhang D., Fernandez-Patron C. cf2@ualberta.ca Only protein-protein interactions in vitro In vitro enzymatic study enzymatic study western blot western blot Immuno blot Immuno blot Wang X., Berry E., Hernandez-Anzaldo S., Sun D., Adijiang A., Li L., Zhang D., Fernandez-Patron C. cf2@ualberta.ca Accepted 2015-APR-28 by LUANA FEBS Lett. (0014-5793) 2015 Only protein-protein interactions imex curation MMP-2 inhibits PCSK9-induced degradation of the LDL receptor in Hepa1-c1c7 cells. MMP-2 inhibits PCSK9-induced degradation of the LDL receptor in Hepa1-c1c7 cells. FEBS Lett. (0014-5793) 2015 imex curation Wang X., Berry E., Hernandez-Anzaldo S., Sun D., Adijiang A., Li L., Zhang D., Fernandez-Patron C. cf2@ualberta.ca Only protein-protein interactions in vitro In vitro bn-page blue native page bn-page western blot western blot Immuno blot Immuno blot Wang X., Berry E., Hernandez-Anzaldo S., Sun D., Adijiang A., Li L., Zhang D., Fernandez-Patron C. cf2@ualberta.ca Accepted 2015-APR-28 by LUANA FEBS Lett. (0014-5793) 2015 Only protein-protein interactions imex curation MMP-2 inhibits PCSK9-induced degradation of the LDL receptor in Hepa1-c1c7 cells. MMP-2 inhibits PCSK9-induced degradation of the LDL receptor in Hepa1-c1c7 cells. FEBS Lett. (0014-5793) 2015 imex curation Wang X., Berry E., Hernandez-Anzaldo S., Sun D., Adijiang A., Li L., Zhang D., Fernandez-Patron C. cf2@ualberta.ca Only protein-protein interactions in vitro In vitro enzymatic study enzymatic study ms participant Identification by mass spectrometry ms participant Wang X., Berry E., Hernandez-Anzaldo S., Sun D., Adijiang A., Li L., Zhang D., Fernandez-Patron C. cf2@ualberta.ca Accepted 2015-APR-28 by LUANA FEBS Lett. (0014-5793) 2015 Only protein-protein interactions imex curation pcsk9_human Proprotein convertase subtilisin/kexin type 9 Subtilisin/kexin-like protease PC9 Neural apoptosis-regulated convertase 1 Proprotein convertase 9 PCSK9 NARC1 PSEC0052 protein protein human Homo sapiens Human MGTVSSRRSWWPLPLLLLLLLLLGPAGARAQEDEDGDYEELVLALRSEEDGLAEAPEHGTTATFHRCAKDPWRLPGTYVVVLKEETHLSQSERTARRLQAQAARRGYLTKILHVFHGLLPGFLVKMSGDLLELALKLPHVDYIEEDSSVFAQSIPWNLERITPPRYRADEYQPPDGGSLVEVYLLDTSIQSDHREIEGRVMVTDFENVPEEDGTRFHRQASKCDSHGTHLAGVVSGRDAGVAKGASMRSLRVLNCQGKGTVSGTLIGLEFIRKSQLVQPVGPLVVLLPLAGGYSRVLNAACQRLARAGVVLVTAAGNFRDDACLYSPASAPEVITVGATNAQDQPVTLGTLGTNFGRCVDLFAPGEDIIGASSDCSTCFVSQSGTSQAAAHVAGIAAMMLSAEPELTLAELRQRLIHFSAKDVINEAWFPEDQRVLTPNLVAALPPSTHGAGWQLFCRTVWSAHSGPTRMATAVARCAPDEELLSCSSFSRSGKRRGERMEAQGGKLVCRAHNAFGGEGVYAIARCCLLPQANCSVHTAPPAEASMGTRVHCHQQGHVLTGCSSHWEVEDLGTHKPPVLRPRGQPNQCVGHREASIHASCCHAPGLECKVKEHGIPAPQEQVTVACEEGWTLTGCSALPGTSHVLGAYAVDNTCVVRSRDVSTTGSTSEGAVTAVAICCRSRHLAQASQELQ mint homomint May be implicated in the differentiation of cortical neurons and may play a role in cholesterol homeostasis Defects in PCSK9 are the cause of familial hypercholesterolemia 3 (FH3) [MIM:603776]. FH3 inheritance is autosomal dominant 9BCB9418B90AEE23 mmp2_human 72 kDa type IV collagenase MMP2 CLG4A 72 kDa gelatinase Matrix metalloproteinase-2 Gelatinase A TBE-1 protein protein human Homo sapiens Human MEALMARGALTGPLRALCLLGCLLSHAAAAPSPIIKFPGDVAPKTDKELAVQYLNTFYGCPKESCNLFVLKDTLKKMQKFFGLPQTGDLDQNTIETMRKPRCGNPDVANYNFFPRKPKWDKNQITYRIIGYTPDLDPETVDDAFARAFQVWSDVTPLRFSRIHDGEADIMINFGRWEHGDGYPFDGKDGLLAHAFAPGTGVGGDSHFDDDELWTLGEGQVVRVKYGNADGEYCKFPFLFNGKEYNSCTDTGRSDGFLWCSTTYNFEKDGKYGFCPHEALFTMGGNAEGQPCKFPFRFQGTSYDSCTTEGRTDGYRWCGTTEDYDRDKKYGFCPETAMSTVGGNSEGAPCVFPFTFLGNKYESCTSAGRSDGKMWCATTANYDDDRKWGFCPDQGYSLFLVAAHEFGHAMGLEHSQDPGALMAPIYTYTKNFRLSQDDIKGIQELYGASPDIDLGTGPTPTLGPVTPEICKQDIVFDGIAQIRGEIFFFKDRFIWRTVTPRDKPMGPLLVATFWPELPEKIDAVYEAPQEEKAVFFAGNEYWIYSASTLERGYPKPLTSLGLPPDVQRVDAAFNWSKNKKTYIFAGDKFWRYNEVKKKMDPGFPKLIADAWNAIPDNLDAVVDLQGGGHSYFFKGAYYLKLENQSLKSVKFGSIKSDWLGC BC7147DC8B49F289 ldlr_human Low-density lipoprotein receptor LDLR protein protein human Homo sapiens Human MGPWGWKLRWTVALLLAAAGTAVGDRCERNEFQCQDGKCISYKWVCDGSAECQDGSDESQETCLSVTCKSGDFSCGGRVNRCIPQFWRCDGQVDCDNGSDEQGCPPKTCSQDEFRCHDGKCISRQFVCDSDRDCLDGSDEASCPVLTCGPASFQCNSSTCIPQLWACDNDPDCEDGSDEWPQRCRGLYVFQGDSSPCSAFEFHCLSGECIHSSWRCDGGPDCKDKSDEENCAVATCRPDEFQCSDGNCIHGSRQCDREYDCKDMSDEVGCVNVTLCEGPNKFKCHSGECITLDKVCNMARDCRDWSDEPIKECGTNECLDNNGGCSHVCNDLKIGYECLCPDGFQLVAQRRCEDIDECQDPDTCSQLCVNLEGGYKCQCEEGFQLDPHTKACKAVGSIAYLFFTNRHEVRKMTLDRSEYTSLIPNLRNVVALDTEVASNRIYWSDLSQRMICSTQLDRAHGVSSYDTVISRDIQAPDGLAVDWIHSNIYWTDSVLGTVSVADTKGVKRKTLFRENGSKPRAIVVDPVHGFMYWTDWGTPAKIKKGGLNGVDIYSLVTENIQWPNGITLDLLSGRLYWVDSKLHSISSIDVNGGNRKTILEDEKRLAHPFSLAVFEDKVFWTDIINEAIFSANRLTGSDVNLLAENLLSPEDMVLFHNLTQPRGVNWCERTTLSNGGCQYLCLPAPQINPHSPKFTCACPDGMLLARDMRSCLTEAEAAVATQETSTVRLKVSSTAVRTQHTTTRPVPDTSRLPGATPGLTTVEIVTMSHQALGDVAGRGNEKKPSSVRALSIVLPIVLLVFLCLGVFLLWKNWRLKNINSINFDNPVYQKTTEDEVHICHNQDGYSYPSRQMVSLEDDVA DIP protein P01130 original sequence version: 115 DIP protein P01130 original sequence version: 122 DIP protein P01130 original sequence version: 140 DIP protein P01130 original sequence version: 173 A4C28E9B8BADAD5E pcsk9_mouse Proprotein convertase subtilisin/kexin type 9 Pcsk9 Narc1 Neural apoptosis-regulated convertase 1 Proprotein convertase 9 Subtilisin/kexin-like protease PC9 protein protein mouse Mus musculus Mouse MGTHCSAWLRWPLLPLLPPLLLLLLLLCPTGAGAQDEDGDYEELMLALPSQEDGLADEAAHVATATFRRCSKEAWRLPGTYIVVLMEETQRLQIEQTAHRLQTRAARRGYVIKVLHIFYDLFPGFLVKMSSDLLGLALKLPHVEYIEEDSFVFAQSIPWNLERIIPAWHQTEEDRSPDGSSQVEVYLLDTSIQGAHREIEGRVTITDFNSVPEEDGTRFHRQASKCDSHGTHLAGVVSGRDAGVAKGTSLHSLRVLNCQGKGTVSGTLIGLEFIRKSQLIQPSGPLVVLLPLAGGYSRILNAACRHLARTGVVLVAAAGNFRDDACLYSPASAPEVITVGATNAQDQPVTLGTLGTNFGRCVDLFAPGKDIIGASSDCSTCFMSQSGTSQAAAHVAGIVARMLSREPTLTLAELRQRLIHFSTKDVINMAWFPEDQQVLTPNLVATLPPSTHETGGQLLCRTVWSAHSGPTRTATATARCAPEEELLSCSSFSRSGRRRGDWIEAIGGQQVCKALNAFGGEGVYAVARCCLVPRANCSIHNTPAARAGLETHVHCHQKDHVLTGCSFHWEVEDLSVRRQPALRSRRQPGQCVGHQAASVYASCCHAPGLECKIKEHGISGPSEQVTVACEAGWTLTGCNVLPGASLTLGAYSVDNLCVARVHDTARADRTSGEATVAAAICCRSRPSAKASWVQ 977BD4BD1FAF98C0 mmp2-pcsk9 1 6 weight by comassie molecular weight estimation by coomasie staining weight by comassie weight by staining molecular weight estimation by staining weight by staining enzyme target enzyme target substrate substrate neutral component neutral component purified purified 7 predetermined predetermined participant predetermined weight by staining molecular weight estimation by staining weight by staining enzyme enzyme neutral component neutral component purified purified cleavage cleavage reaction cleavage f2a f2c f4a f5b mmp2-pcsk9-1 5 9 ms participant Identification by mass spectrometry ms participant ms participant Identification by mass spectrometry ms participant enzyme target enzyme target substrate substrate neutral component neutral component purified purified gly397 modres protein modification ModRes modified residue ms mass detection of residue modification modified residue ms certain certain sequence position certain certain certain sequence position certain gly617 modres protein modification ModRes modified residue ms mass detection of residue modification modified residue ms certain certain sequence position certain certain certain sequence position certain 7 predetermined predetermined participant predetermined ms participant Identification by mass spectrometry ms participant enzyme enzyme neutral component neutral component purified purified cleavage cleavage reaction cleavage sf2 f2b st2 two putative cleavage sites both occurring at Gly;Ile bonds proximal to the C-terminus of PCSK9 (Supplementary Fig. 2) ldlr-mmp2-1 4 6 western blot western blot Immuno blot Immuno blot western blot western blot Immuno blot Immuno blot unspecified role unspecified role neutral component neutral component purified purified 7 predetermined predetermined participant predetermined western blot western blot Immuno blot Immuno blot unspecified role unspecified role neutral component neutral component purified purified 8 predetermined predetermined participant predetermined western blot western blot Immuno blot Immuno blot unspecified role unspecified role neutral component neutral component purified purified range binding region binding-associated region binding region inferred by curator inferred by curator modelled by curator modeled by curator certain certain sequence position certain certain certain sequence position certain physical association physical association f3 mmp2-pcsk9-2 2 6 weight by comassie molecular weight estimation by coomasie staining weight by comassie weight by comassie molecular weight estimation by coomasie staining weight by comassie enzyme target enzyme target substrate substrate neutral component neutral component purified purified 7 predetermined predetermined participant predetermined weight by comassie molecular weight estimation by coomasie staining weight by comassie enzyme enzyme neutral component neutral component purified purified cleavage cleavage reaction cleavage f5a f3 mmp2-pcsk9-3 3 6 anti tag western anti tag western blot anti tag western western blot western blot Immuno blot Immuno blot enzyme target enzyme target substrate substrate neutral component neutral component purified purified his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 7 western blot western blot Immuno blot Immuno blot western blot western blot Immuno blot Immuno blot enzyme enzyme neutral component neutral component purified purified cleavage cleavage reaction cleavage f5c mmp2-pcsk9-4 4 6 unspecified role unspecified role neutral component neutral component purified purified 7 predetermined predetermined participant predetermined western blot western blot Immuno blot Immuno blot unspecified role unspecified role neutral component neutral component purified purified direct interaction direct interaction f3