IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ LRRK2 functions in synaptic vesicle endocytosis through a kinase-dependent mechanism. LRRK2 functions in synaptic vesicle endocytosis through a kinase-dependent mechanism. J. Cell. Sci. (0021-9533) 2014 imex curation Arranz A.M., Delbroek L., Van Kolen K., Guimarães M.R., Mandemakers W., Daneels G., Matta S., Calafate S., Shaban H., Baatsen P., De Bock P.J., Gevaert K., Vanden Berghe P., Verstreken P., De Strooper B., Moechars D. Parkinsons - Interactions investigated in the context of Parkinsons disease Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. kvkolen@its.jnj.com Only protein-protein interactions in vitro In vitro protein kinase assay protein kinase assay sequence tag sequence tag identification sequence tag Arranz A.M., Delbroek L., Van Kolen K., Guimarães M.R., Mandemakers W., Daneels G., Matta S., Calafate S., Shaban H., Baatsen P., De Bock P.J., Gevaert K., Vanden Berghe P., Verstreken P., De Strooper B., Moechars D. Parkinsons - Interactions investigated in the context of Parkinsons disease The isoform, exact sequence used, tag location and expression system are not known for the substrate proteins. When details are unambiguous this info has been entered; otherwise canonical isoforms have been annotated here. kvkolen@its.jnj.com Accepted 2015-MAR-03 by ORCHARD J. Cell. Sci. (0021-9533) 2014 imex curation LRRK2 functions in synaptic vesicle endocytosis through a kinase-dependent mechanism. LRRK2 functions in synaptic vesicle endocytosis through a kinase-dependent mechanism. J. Cell. Sci. (0021-9533) 2014 imex curation Arranz A.M., Delbroek L., Van Kolen K., Guimarães M.R., Mandemakers W., Daneels G., Matta S., Calafate S., Shaban H., Baatsen P., De Bock P.J., Gevaert K., Vanden Berghe P., Verstreken P., De Strooper B., Moechars D. Parkinsons - Interactions investigated in the context of Parkinsons disease Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. kvkolen@its.jnj.com Only protein-protein interactions in vitro In vitro protein kinase assay protein kinase assay autoradiography autoradiography Arranz A.M., Delbroek L., Van Kolen K., Guimarães M.R., Mandemakers W., Daneels G., Matta S., Calafate S., Shaban H., Baatsen P., De Bock P.J., Gevaert K., Vanden Berghe P., Verstreken P., De Strooper B., Moechars D. Parkinsons - Interactions investigated in the context of Parkinsons disease The isoform, exact sequence used, tag location and expression system are not known for the substrate proteins. When details are unambiguous this info has been entered; otherwise canonical isoforms have been annotated here. Interactions involving GSK3B have not been entered as the origins of this protein are not known. kvkolen@its.jnj.com Accepted 2015-MAR-17 by ORCHARD J. Cell. Sci. (0021-9533) 2014 Only protein-protein interactions imex curation In vitro phosphorylation assay states "GST tagged substrates". -which means all of them - the Human Endophilin A1 (Abnova ones) state the tag is N-terminal. I have assumed AbNOva supplied this protein as Dario Alessi - the other "supplier" of proteins does not have any publications with this mol. However he does use GSK3B -so possibly supplied it- so have removed this GSK interaction and added a Data Proc statement. sh3g2_human Endophilin-A1 SH3GL2 CNSA2 SH3D2A Endophilin-1 SH3 domain-containing GRB2-like protein 2 SH3 domain protein 2A EEN-B1 protein protein human Homo sapiens Human MSVAGLKKQFHKATQKVSEKVGGAEGTKLDDDFKEMERKVDVTSRAVMEIMTKTIEYLQPNPASRAKLSMINTMSKIRGQEKGPGYPQAEALLAEAMLKFGRELGDDCNFGPALGEVGEAMRELSEVKDSLDIEVKQNFIDPLQNLHDKDLREIQHHLKKLEGRRLDFDYKKKRQGKIPDEELRQALEKFDESKEIAESSMFNLLEMDIEQVSQLSALVQAQLEYHKQAVQILQQVTVRLEERIRQASSQPRREYQPKPRMSLEFPTGDSTQPNGGLSHTGTPKPSGVQMDQPCCRALYDFEPENEGELGFKEGDIITLTNQIDENWYEGMLHGHSGFFPINYVEILVALPH DIP protein Q99962 original sequence version: 111 DIP protein Q99962 original sequence version: 141 F3AE2353E1EB7CF3 lrrk2_human Leucine-rich repeat serine/threonine-protein kinase 2 LRRK2 PARK8 Dardarin protein protein human Homo sapiens Human MASGSCQGCEEDEETLKKLIVRLNNVQEGKQIETLVQILEDLLVFTYSERASKLFQGKNIHVPLLIVLDSYMRVASVQQVGWSLLCKLIEVCPGTMQSLMGPQDVGNDWEVLGVHQLILKMLTVHNASVNLSVIGLKTLDLLLTSGKITLLILDEESDIFMLIFDAMHSFPANDEVQKLGCKALHVLFERVSEEQLTEFVENKDYMILLSALTNFKDEEEIVLHVLHCLHSLAIPCNNVEVLMSGNVRCYNIVVEAMKAFPMSERIQEVSCCLLHRLTLGNFFNILVLNEVHEFVVKAVQQYPENAALQISALSCLALLTETIFLNQDLEEKNENQENDDEGEEDKLFWLEACYKALTWHRKNKHVQEAACWALNNLLMYQNSLHEKIGDEDGHFPAHREVMLSMLMHSSSKEVFQASANALSTLLEQNVNFRKILLSKGIHLNVLELMQKHIHSPEVAESGCKMLNHLFEGSNTSLDIMAAVVPKILTVMKRHETSLPVQLEALRAILHFIVPGMPEESREDTEFHHKLNMVKKQCFKNDIHKLVLAALNRFIGNPGIQKCGLKVISSIVHFPDALEMLSLEGAMDSVLHTLQMYPDDQEIQCLGLSLIGYLITKKNVFIGTGHLLAKILVSSLYRFKDVAEIQTKGFQTILAILKLSASFSKLLVHHSFDLVIFHQMSSNIMEQKDQQFLNLCCKCFAKVAMDDYLKNVMLERACDQNNSIMVECLLLLGADANQAKEGSSLICQVCEKESSPKLVELLLNSGSREQDVRKALTISIGKGDSQIISLLLRRLALDVANNSICLGGFCIGKVEPSWLGPLFPDKTSNLRKQTNIASTLARMVIRYQMKSAVEEGTASGSDGNFSEDVLSKFDEWTFIPDSSMDSVFAQSDDLDSEGSEGSFLVKKKSNSISVGEFYRDAVLQRCSPNLQRHSNSLGPIFDHEDLLKRKRKILSSDDSLRSSKLQSHMRHSDSISSLASEREYITSLDLSANELRDIDALSQKCCISVHLEHLEKLELHQNALTSFPQQLCETLKSLTHLDLHSNKFTSFPSYLLKMSCIANLDVSRNDIGPSVVLDPTVKCPTLKQFNLSYNQLSFVPENLTDVVEKLEQLILEGNKISGICSPLRLKELKILNLSKNHISSLSENFLEACPKVESFSARMNFLAAMPFLPPSMTILKLSQNKFSCIPEAILNLPHLRSLDMSSNDIQYLPGPAHWKSLNLRELLFSHNQISILDLSEKAYLWSRVEKLHLSHNKLKEIPPEIGCLENLTSLDVSYNLELRSFPNEMGKLSKIWDLPLDELHLNFDFKHIGCKAKDIIRFLQQRLKKAVPYNRMKLMIVGNTGSGKTTLLQQLMKTKKSDLGMQSATVGIDVKDWPIQIRDKRKRDLVLNVWDFAGREEFYSTHPHFMTQRALYLAVYDLSKGQAEVDAMKPWLFNIKARASSSPVILVGTHLDVSDEKQRKACMSKITKELLNKRGFPAIRDYHFVNATEESDALAKLRKTIINESLNFKIRDQLVVGQLIPDCYVELEKIILSERKNVPIEFPVIDRKRLLQLVRENQLQLDENELPHAVHFLNESGVLLHFQDPALQLSDLYFVEPKWLCKIMAQILTVKVEGCPKHPKGIISRRDVEKFLSKKRKFPKNYMSQYFKLLEKFQIALPIGEEYLLVPSSLSDHRPVIELPHCENSEIIIRLYEMPYFPMGFWSRLINRLLEISPYMLSGRERALRPNRMYWRQGIYLNWSPEAYCLVGSEVLDNHPESFLKITVPSCRKGCILLGQVVDHIDSLMEEWFPGLLEIDICGEGETLLKKWALYSFNDGEEHQKILLDDLMKKAEEGDLLVNPDQPRLTIPISQIAPDLILADLPRNIMLNNDELEFEQAPEFLLGDGSFGSVYRAAYEGEEVAVKIFNKHTSLRLLRQELVVLCHLHHPSLISLLAAGIRPRMLVMELASKGSLDRLLQQDKASLTRTLQHRIALHVADGLRYLHSAMIIYRDLKPHNVLLFTLYPNAAIIAKIADYGIAQYCCRMGIKTSEGTPGFRAPEVARGNVIYNQQADVYSFGLLLYDILTTGGRIVEGLKFPNEFDELEIQGKLPDPVKEYGCAPWPMVEKLIKQCLKENPQERPTSAQVFDILNSAELVCLTRRILLPKNVIVECMVATHHNSRNASIWLGCGHTDRGQLSFLDLNTEGYTSEEVADSRILCLALVHLPVEKESWIVSGTQSGTLLVINTEDGKKRHTLEKMTDSVTCLYCNSFSKQSKQKNFLLVGTADGKLAIFEDKTVKLKGAAPLKILNIGNVSTPLMCLSESTNSTERNVMWGGCGTKIFSFSNDFTIQKLIETRTSQLFSYAAFSDSNIITVVVDTALYIAKQNSPVVEVWDKKTEKLCGLIDCVHFLREVMVKENKESKHKMSYSGRVKTLCLQKNTALWIGTGGGHILLLDLSTRRLIRVIYNFCNSVRVMMTAQLGSLKNVMLVLGYNRKNTEGTQKQKEIQSCLTVWDINLPHEVQNLEKHIEVRKELAEKMRRTSVE DIP protein Q5S007 original sequence version: 36 DIP protein Q5S007 original sequence version: 68 DIP protein Q5S007 original sequence version: 64 26142A0CECBBC3F4 lrrk2-sh3gl2-2 2 4 enzyme enzyme neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain Q5S007:p.Gly2019Ser mutation increasing rate mutation increasing interaction rate mutation increasing rate certain certain sequence position certain certain certain sequence position certain G S Wed May 02 20:04:05 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gly2019ser' Tue Jun 30 19:47:10 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly2019Ser' Q5S007:p.Asp1994Ala mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain D A Wed May 02 15:41:24 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp1994ala' Tue Jun 30 16:10:15 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp1994Ala' region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 3 enzyme target enzyme target substrate substrate neutral component neutral component Q99962:p.Thr73Ala mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain T A Wed May 02 17:36:51 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'thr73ala' Tue Jun 30 17:04:06 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Thr73Ala' Q99962:p.[Thr73Ala;Ser75Ala] mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain S A certain certain sequence position certain certain certain sequence position certain T A Fri Jul 22 11:10:30 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'ser75ala,thr73ala' Wed May 02 15:38:38 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'thr73ala,ser75ala' Tue Jun 30 16:43:00 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Thr73Ala;Ser75Ala]' Q99962:p.Ser75Ala mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain S A Wed May 02 16:11:16 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser75ala' Tue Jun 30 17:32:31 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser75Ala' n_terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range in vitro In vitro phosphorylation phosphorylation reaction phosphorylation 5,6, Supp fig 2 Q99962 ser-75 O-phospho-L-serine Q99962 thr-73 O-phospho-L-threonine Phosphorylation of double mutant EndoA1 was not significantly different from phosphorylation of T73A or S75A EndoA1. lrrk2-28 2 4 putative self putative self putative self putative self Q5S007:p.Gly2019Ser mutation increasing rate mutation increasing interaction rate mutation increasing rate certain certain sequence position certain certain certain sequence position certain G S Wed May 02 15:19:41 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gly2019ser' Tue Jun 30 20:17:32 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly2019Ser' Q5S007:p.Asp1994Ala mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain D A Wed May 02 14:48:10 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp1994ala' Tue Jun 30 20:19:16 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp1994Ala' region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain true region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined true phosphorylation phosphorylation reaction phosphorylation true 5,6, Supp Fig 2 Q5S007 phophores lrrk2-3 1 4 putative self putative self putative self putative self region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain true Q5S007:p.Asp1994Ala mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain true D A Wed May 02 20:16:55 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp1994ala' Tue Jun 30 18:24:53 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp1994Ala' region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined true Q5S007:p.Gly2019Ser mutation increasing rate mutation increasing interaction rate mutation increasing rate certain certain sequence position certain certain certain sequence position certain true G S Wed May 02 19:40:13 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gly2019ser' Tue Jun 30 19:09:21 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly2019Ser' phosphorylation phosphorylation reaction phosphorylation true Table S1 Q5S007 thr-985 O-phospho-L-threonine Q5S007 ser-992 O-phospho-L-serine Q5S007 ser-1058 O-phospho-L-serine Q5S007 ser-1443 O-phospho-L-serine On the table some phosphosites have been entered as individual phosphosites - but give results for multiple sites e.g. "1443". - no I have idea what this is meant to mean (if anything) , so have listed sites individually (cannot give res-ptm any other way)... there are 3 serines at 1443-5 - so presumably only 2 of 3 listed in LHS column as the other (1445) did not make the 99% confidence level??) Q5S007 ser-1444 O-phospho-L-serine Q5S007 ser-1467 O-phospho-L-serine Q5S007 ser-1508 O-phospho-L-serine Q5S007 ser-1636 O-phospho-L-serine Q5S007 ser-1951 O-phospho-L-serine Q5S007 ser-2032 O-phospho-L-serine Q5S007 thr-985 O-phospho-L-threonine Q5S007 thr-1343 O-phospho-L-threonine Q5S007 thr-1357 O-phospho-L-threonine Q5S007 thr-1368 O-phospho-L-threonine Q5S007 thr-1452 O-phospho-L-threonine Q5S007 thr-1491 O-phospho-L-threonine Q5S007 thr-1470 O-phospho-L-threonine Q5S007 thr-1503 O-phospho-L-threonine Q5S007 thr-1967 O-phospho-L-threonine Q5S007 thr-2035 O-phospho-L-threonine Q5S007 thr-2031 O-phospho-L-threonine Q5S007 thr-2267 O-phospho-L-threonine Q5S007 tyr-1331 O-phospho-L-tyrosine Q5S007 tyr-1485 O-phospho-L-tyrosine lrrk2-sh3gl2-3 1 3 enzyme target enzyme target substrate substrate neutral component neutral component n_terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range true in vitro In vitro 4 enzyme enzyme neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain true region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined true phosphorylation phosphorylation reaction phosphorylation Table S1 ser-75 O-phospho-L-serine thr-73 O-phospho-L-threonine