IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Surveillance of nuclear pore complex assembly by ESCRT-III/Vps4. Surveillance of nuclear pore complex assembly by ESCRT-III/Vps4. Cell (0092-8674) 2014 imex curation Webster B.M., Colombi P., Jäger J., Lusk C.P. Colocalisation data has not been added as I think all these just show subcellular location information. patrick.lusk@yale.edu 22-Jan-2015: Contacted by IntAct-Help. Only protein-protein interactions yeast Saccharomyces cerevisiae Baker's yeast fluorescence imaging fluorescence microscopy fluorescence imaging tag fluorescence tag visualisation by fluorescence tag fluorescence Webster B.M., Colombi P., Jäger J., Lusk C.P. Colocalisation data has not been added as I think all these just show subcellular location information. patrick.lusk@yale.edu Accepted 2015-JAN-15 by MGT Cell (0092-8674) 2014 Only protein-protein interactions imex curation Surveillance of nuclear pore complex assembly by ESCRT-III/Vps4. Surveillance of nuclear pore complex assembly by ESCRT-III/Vps4. Cell (0092-8674) 2014 imex curation Webster B.M., Colombi P., Jäger J., Lusk C.P. Colocalisation data has not been added as I think all these just show subcellular location information. patrick.lusk@yale.edu 22-Jan-2015: Contacted by IntAct-Help. Only protein-protein interactions yeast Saccharomyces cerevisiae Baker's yeast anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Webster B.M., Colombi P., Jäger J., Lusk C.P. Colocalisation data has not been added as I think all these just show subcellular location information. patrick.lusk@yale.edu Accepted 2015-JAN-15 by MGT Cell (0092-8674) 2014 Only protein-protein interactions imex curation Surveillance of nuclear pore complex assembly by ESCRT-III/Vps4. Surveillance of nuclear pore complex assembly by ESCRT-III/Vps4. Cell (0092-8674) 2014 imex curation Webster B.M., Colombi P., Jäger J., Lusk C.P. Colocalisation data has not been added as I think all these just show subcellular location information. patrick.lusk@yale.edu 22-Jan-2015: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Webster B.M., Colombi P., Jäger J., Lusk C.P. Colocalisation data has not been added as I think all these just show subcellular location information. patrick.lusk@yale.edu Accepted 2015-JAN-15 by MGT Cell (0092-8674) 2014 Only protein-protein interactions imex curation Surveillance of nuclear pore complex assembly by ESCRT-III/Vps4. Surveillance of nuclear pore complex assembly by ESCRT-III/Vps4. Cell (0092-8674) 2014 imex curation Webster B.M., Colombi P., Jäger J., Lusk C.P. Colocalisation data has not been added as I think all these just show subcellular location information. patrick.lusk@yale.edu 22-Jan-2015: Contacted by IntAct-Help. Only protein-protein interactions yeast Saccharomyces cerevisiae Baker's yeast bifc bimolecular fluorescence complementation bifc tag fluorescence tag visualisation by fluorescence tag fluorescence Webster B.M., Colombi P., Jäger J., Lusk C.P. Colocalisation data has not been added as I think all these just show subcellular location information. patrick.lusk@yale.edu Accepted 2015-JAN-15 by MGT Cell (0092-8674) 2014 Only protein-protein interactions imex curation vps20_yeast Vacuolar protein sorting-associated protein 20 ASI10 CHM6 VPT20 YM9582.02C YMR077C VPS20 Vacuolar protein-targeting protein 20 Amino acid sensor-independent protein 10 Charged multivesicular body protein 6 ESCRT-III complex subunit VPS20 protein protein yeast Saccharomyces cerevisiae Baker's yeast MGQKSSKVHITKTDRAILEVKRSKDEIHKFTRRTDNLILVEKSQLKDLIRKNPENYKSNMKVRFLLKRIHYQEHLLQQASDQLINLENMVSTLEFKMVEKQFINGLKNGNEILKKLNKEFSNVDELMDDVQDQIAYQNEINETLSRSLVGTSNYEDDLDKELDALESELNPEKMNNAKVANMPSTEGLPSLPQGEQTEQKEREEFATEERSDTKEPLALLS CDC88BC4ADA3D602 CDC88BC4ADA3D602 heh2_yeast Inner nuclear membrane protein HEH2 YDR458C D8035.2 HEH2 Helix-extension-helix domain-containing protein 2 protein protein yeast Saccharomyces cerevisiae Baker's yeast MDHRNLDPKTLKVSQLRRVLVENDVAFPANARKPVLVKLFEEKVRQRLQSSPEASKVRTSIQKVVKSGAKNADRKKTLKSKKLESSSSESKTVKDENVETNKRKREQISTDNEAKMQIQEEKSPKKKRKKRSSKANKPPESPPQSKSDGKATSADLTSELETVEELHKKDSSDDKPRVKELPKPELPNLKVSNEFLAQLNKELASAATENYDHSIKSTDLSSIRIETEEPVGPSTGAETRNESEVMENINLEVQPEVKEAKEELTKISETFDNQDEEDTSRLSSKKNIRSPKGRTRHFIANKTKRGIDIMKPFIAHLFIWLWNGAIFLSIICPILFGLWYREQRIQVGYCGHEKPLKSLAISAFPQTERVDSVLQAYRPNCLECPEHGICSSFMNVECEPGYEPKSSILETYGIIPFPKYCAKDESKEKEVDELVWKVNEYLKKKNAQHECGEGENLFESGETETKLYDIFSHSRPSWESQREFNDHWKNVLEILKKKDDIIWLPLDFETNGKREKSKSNNTNYIYRSTSKKWVTLQCHLEGDIQEYITKYGGSLFITLGVLFLIKKIQSTLDNYVQGEQIIEKLVKEAIDKLKDVKKNKGEEPFLTTVQLRATLLSDIPNIKEQNNLWAQTKEKIMKEQSENIELYLLEENGEIMTCWEWKE 1E11821F2542723E 1E11821F2542723E nu170_yeast Nucleoporin NUP170 NUP170 NLE3 YBL079W YBL0725 Nuclear pore protein NUP170 protein protein yeast Saccharomyces cerevisiae Baker's yeast MFQSFFHNNGPAAAGETFSDSRSYPLTNHQEVPRNGLNELASSATKAQQQPTHILNSYPITGSNPLMRASAMGATSGSINPNMSNMNEHIRVSGMGTSKPLDLAGKYIDHLQHKDSNTPVLDERSYYNSGVDYNFSREKNGLGAFTPFEKQDVFNIPDEILHEFSTSQTKTDMGIFPELNRCWITIDNKLILWNINNDNEYQVVDDMKHTIQKVALVRPKPNTFVPAVKHLLLISTTMELFMFAISLDKATNELSVFNTHLSVPVQGIDVIDIVSHERSGRIFFAGQASGLNIWELHYSGSDDWFNSKCSKVCLTKSALLSLLPTNMLSQIPGVDFIQALFEDNSNGNGGFSQETITQLTIDQQRGIIYSLSSKSTIRAYVITEKSLEGPMSIEPAYISRIIGTTTARAAPILGPKYLKIVKISSVAPEENNNLFLVALTVGGVRLYFNGSMGRFNIEALRLESIKFPPSSVTPEVIQQELLHQQQEQAKRSFPFFSNLMSSEPVLLKFQKKSSVLLETTKASTIISPGIFFSAVIKSSQQTHQQEKKENSSVTGTTATAGSKTVKQQPVTLQHKLFVSVPDYGILKTHGKYVENATFLETAGPVQQIIPLSGLFNATTKPQGFANEFATQYTSETLRVAVLTSTSIEIYKYRTPDEIFEDLIDNPLPFVLNYGAAEACSTALFVTCKSNKSEKLRSNALTFLTMGIPGVVDIKPVYNRYSVSTVSSLLSKPTLSTATTNLQQSITGFSKPSPANKEDFDLDDVILSPRFYGIALLITRLLRDIWGRHVFMTFTDNRVTSHAFISSSDPITPSINNLKSDEISQNRNIISKVSISKDCIEYYLSSINILNEFFITYGDSISQISAPYVLANNSNGRVIDKTEEVANQAESIAINAMIKMVQSIKEGLSFLNVLYEESEVEGFDNQYLGFKDIISFVSLDVQKDLVKLDFKDLFAPNDKTKSLIREILLSIINRNITKGASIEYTATALQERCGSFCSASDILGFRAIEHLRRAKEIGLRNYDSLNYHLKNATALLEQIVDDLSIEKLKEAVSMMLSVNYYPKSIEFLLNIANSMDKGKLACQYVANGFLENDDRKQYYDKRILVYDLVFDTLIKVDELAEKKQSSKTQNQISISNDDEVKLRQKSYEAALKYNDRLFHYHMYDWLVSQNREEKLLDIETPFILPYLMEKAGSSLKISNILWVYYSRRSKFFESAEILYRLATSNFDITLFERIEFLSRANGFCNSVSPLSQKQRIVQLASRIQDACEVAGIQGDILSLVYTDARIDSAIKDELIKTLDGKILSTSELFNDFAVPLSYHEIALFIFKIADFRDHEVIMAKWDELFQSLRMEFNNTGKKEDSMNFINLLSNVLIKIGKNVQDSEFIFPIFELFPIVCNFFYETLPKEHIVSGSIVSIFITAGVSFNKMYYILKELIETSDSDNSVFNKEMTWLIHEWYKSDRKFRDIISYNDIIHLKEYKIDNDPIEKYVKNSGNNLGICFYKE 3BEA65DAA2A5F99A 3BEA65DAA2A5F99A snf7_yeast Vacuolar-sorting protein SNF7 SNF7 VPS32 YLR025W DID1 Vacuolar protein-sorting-associated protein 32 DOA4-independent degradation protein 1 Sucrose nonfermenting protein 7 protein protein yeast Saccharomyces cerevisiae Baker's yeast MWSSLFGWTSSNAKNKESPTKAIVRLREHINLLSKKQSHLRTQITNQENEARIFLTKGNKVMAKNALKKKKTIEQLLSKVEGTMESMEQQLFSIESANLNLETMRAMQEGAKAMKTIHSGLDIDKVDETMDEIREQVELGDEISDAISRPLITGANEVDEDELDEELDMLAQENANQETSKIVNNNVNAAPISENKVSLPSVPSNKIKQSENSVKDGEEEEDEEDEDEKALRELQAEMGL 5241A18BB181F0C1 5241A18BB181F0C1 src1_yeast Inner nuclear membrane protein SRC1 YML033W YML034W SRC1 HEH1 Helix-extension-helix domain-containing protein 1 protein protein yeast Saccharomyces cerevisiae Baker's yeast MNSDLEYLEDGFDPNSMKVATLRRILVENNVDFPSNARKNALVGLFDEKVKPQIPQLRKMYLNVRPSDEGIVKMDRPSSSPSIASPRRSRRARREKSASPMAKQFKKNRILDDVSNDDDDDDDDDDDNDKKDDPLIVPSGTDTDEVDDEEDDVITSSSNKSDTNDFQQNSDTRKKRKDPDSDDWSESNSKENKIDNKHLNLLSSDSEIEQDYQKAKKRKTSDLNQEHGNGSAILGKLSVKTPIKNTNRKPVSMDNFNDSLTSSGTENDPFVPNIRHNPKELGTANGTGHSTPLSKLKVSASFADKLPQKEVPSTILVPEVEQQEPSQSERTPSLFSSEGSGSESEAPLLPEITTPGPHQPMGNTSNNVVEMIDTDSSNLVSDEDEVLVPTRIETPQLPTEKDVEKCEARVQELQEEVNEQLEHENGSEFDVKQGSGKVGNRHKFKRALKFLSKSLLALFLFCIFIVIPLLFGLWYREQRLLIGYCGHEVPSHRVSGNSFEFIQKLDNLLQDYRPKCIPCPPNGICYPYLKLKCKPDYKLAPSRLDFLEIIPAQGKCVKDDKKQQLVSEVVEKSLEFLRAKNAQISCGDGKDDIESGMTEDALYQIFNEARAPWIRDDEFEDLWIQVIKDLTEEPEILWRQLSPTDNNIGGNSNNIIKTNDVPRQKRHLPEKFISKTRNFRSTSKKYIGMKCRFEREIYQTYKKFQRPIWLMFLLIVISKVIEIKLKNYYRKKARIEELVTQTMEKLKFQKIKSMSDPKENAYLSIVQLRDIFLSDIVDLKYKNQLWSEVVKYLEHNNSNIKSNLTEIRGEIMKCWEWIGPMELNEPKDSAENKI 214CA064ECE160F4 214CA064ECE160F4 vps4_yeast Vacuolar protein sorting-associated protein 4 VPS4 CSC1 END13 GRD13 DID6 Protein END13 DOA4-independent degradation protein 6 Vacuolar protein-targeting protein 10 VPL4 VPT10 P9705.10 YPR173C protein protein yeast Saccharomyces cerevisiae Baker's yeast MSTGDFLTKGIELVQKAIDLDTATQYEEAYTAYYNGLDYLMLALKYEKNPKSKDLIRAKFTEYLNRAEQLKKHLESEEANAAKKSPSAGSGSNGGNKKISQEEGEDNGGEDNKKLRGALSSAILSEKPNVKWEDVAGLEGAKEALKEAVILPVKFPHLFKGNRKPTSGILLYGPPGTGKSYLAKAVATEANSTFFSVSSSDLVSKWMGESEKLVKQLFAMARENKPSIIFIDEVDALTGTRGEGESEASRRIKTELLVQMNGVGNDSQGVLVLGATNIPWQLDSAIRRRFERRIYIPLPDLAARTTMFEINVGDTPCVLTKEDYRTLGAMTEGYSGSDIAVVVKDALMQPIRKIQSATHFKDVSTEDDETRKLTPCSPGDDGAIEMSWTDIEADELKEPDLTIKDFLKAIKSTRPTVNEDDLLKQEQFTRDFGQEGN E65BCDB19C9510DD E65BCDB19C9510DD bro1_yeast Vacuolar-sorting protein BRO1 ASI6 NPI3 VPS31 YPL084W BRO1 LPF2 BRO domain-containing protein 1 BCK1-like resistance to osmotic shock protein 1 Vacuolar protein-sorting-associated protein 31 Amino acid sensor-independent protein 6 Nitrogen permease inactivating protein 3 protein protein yeast Saccharomyces cerevisiae Baker's yeast MKPYLFDLKLKDTEKLDWKKGLSSYLKKSYGSSQWRTFYDEKATSELDHLRNNANGELAPSSLSEQNLKYYSFLEHLYFRLGSKGSRLKMDFTWYDAEYSSAQKGLKYTQHTLAFEKSCTLFNIAVIFTQIARENINEDYKNSIANLTKAFSCFEYLSENFLNSPSVDLQSENTRFLANICHAEAQELFVLKLLNDQISSKQYTLISKLSRATCNLFQKCHDFMKEIDDDVAIYGEPKWKTTVTCKLHFYKSLSAYYHGLHLEEENRVGEAIAFLDFSMQQLISSLPFKTWLVEFIDFDGFKETLEKKQKELIKDNDFIYHESVPAVVQVDSIKALDAIKSPTWEKILEPYMQDVANKCDSLYRGIIPLDVYEKESIYSEEKATLLRKQVEETETANLEYSSFIEFTNLPRLLSDLEKQFSDGNIFSNTDTQGQLMRDQIQTWCKFIQTNEFRDIEEQMNKIVFKRKQILEILSALPNDQKENVTKLKSSLVAASNSDEKLFACVKPHIVEINLLNDNGKIWKKFDEFNRNTPPQPSLLDIDDTKNDKILELLKQVKGHAEDLRTLKEERSRNLSELRDEINNDDITKLLIINKGKSDVELKDLFEVELEKFEPLSTRIEATIYKQSSMIDDIKAKLDEIFHLSNFKDKSSGEEKFLEDRKNFFDKLQEAVKSFSIFASDLPKGIEFYDSLFNMSRDLAERVRVAKQTEDSTANSPAPPLPPLDSKASVVGGPPLLPQKSAAFQSLSRQGLNLGDQFQNLKISAGSDLPQGPGIPPRTYEASPYAATPTMAAPPVPPKQSQEDMYDLRRRKAVENEERELQENPTSFYNRPSVFDENMYSKYSS 34DE0CDD2F4AE55E snf7-vps20 4 8 unspecified role unspecified role neutral component neutral component c-terminal venus-n venus n-terminal fragment c-term range C-terminal range c-term range c-term range C-terminal range c-term range 5 unspecified role unspecified role neutral component neutral component c-terminal venus-c venus c-terminal fragment c-term range C-terminal range c-term range c-term range C-terminal range c-term range physical association physical association Fig. 2B bro1-snf7-1 4 8 unspecified role unspecified role neutral component neutral component c-terminal venus-n venus n-terminal fragment c-term range C-terminal range c-term range c-term range C-terminal range c-term range 11 unspecified role unspecified role neutral component neutral component c-terminal venus-c venus c-terminal fragment c-term range C-terminal range c-term range c-term range C-terminal range c-term range physical association physical association Fig. 2B snf7-src1 4 8 unspecified role unspecified role neutral component neutral component c-terminal venus-c venus c-terminal fragment c-term range C-terminal range c-term range c-term range C-terminal range c-term range 9 unspecified role unspecified role neutral component neutral component c-terminal venus-n venus n-terminal fragment c-term range C-terminal range c-term range c-term range C-terminal range c-term range physical association physical association Fig. 2B heh2-snf7 4 6 unspecified role unspecified role neutral component neutral component c-terminal venus-n venus n-terminal fragment c-term range C-terminal range c-term range c-term range C-terminal range c-term range 8 unspecified role unspecified role neutral component neutral component c-terminal venus-c venus c-terminal fragment c-term range C-terminal range c-term range c-term range C-terminal range c-term range physical association physical association Fig. 2B nup170-snf7 4 7 unspecified role unspecified role neutral component neutral component c-terminal venus-c venus c-terminal fragment c-term range C-terminal range c-term range c-term range C-terminal range c-term range 8 unspecified role unspecified role neutral component neutral component c-terminal venus-n venus n-terminal fragment c-term range C-terminal range c-term range c-term range C-terminal range c-term range physical association physical association Fig. 2B nup170-src1 4 7 unspecified role unspecified role neutral component neutral component c-terminal venus-c venus c-terminal fragment c-term range C-terminal range c-term range c-term range C-terminal range c-term range 9 unspecified role unspecified role neutral component neutral component c-terminal venus-n venus n-terminal fragment c-term range C-terminal range c-term range c-term range C-terminal range c-term range physical association physical association Fig. 2B heh2-nup170 4 6 unspecified role unspecified role neutral component neutral component c-terminal venus-n venus n-terminal fragment c-term range C-terminal range c-term range c-term range C-terminal range c-term range 7 unspecified role unspecified role neutral component neutral component c-terminal venus-c venus c-terminal fragment c-term range C-terminal range c-term range c-term range C-terminal range c-term range physical association physical association Fig. 2B heh2-snf7-1 2 8 unspecified role unspecified role prey prey 6 unspecified role unspecified role bait bait region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Fig. 3A This interaction was only detected in the absence of Vps4. heh2-snf7-2 3 6 unspecified role unspecified role bait bait n-terminal extracellular domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain range range region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 8 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined yeast Saccharomyces cerevisiae Baker's yeast physical association physical association Fig. 3C, 3D heh2-vps4 3 10 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined yeast Saccharomyces cerevisiae Baker's yeast 6 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined n-terminal extracellular domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain range range ecoli Escherichia coli (strain K12) physical association physical association Fig. 3D heh2-nup170-1 1 7 unspecified role unspecified role neutral component neutral component c-terminal mcherry mcherry fluorescent protein tag mcherry c-term range C-terminal range c-term range c-term range C-terminal range c-term range 6 unspecified role unspecified role neutral component neutral component c-terminal gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range colocalization colocalization Supp. Fig. 4 in vps4-Deltapom152-Delta cells heh2-nup170-2 1 8 unspecified role unspecified role neutral component neutral component c-terminal gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 7 unspecified role unspecified role neutral component neutral component c-terminal mcherry mcherry fluorescent protein tag mcherry c-term range C-terminal range c-term range c-term range C-terminal range c-term range colocalization colocalization Supp. Fig. 3 in vps4-Deltapom152-Delta cells