IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Rio1 mediates ATP-dependent final maturation of 40S ribosomal subunits. Rio1 mediates ATP-dependent final maturation of 40S ribosomal subunits. Nucleic acids research 2014 imex curation Turowski TW., Lebaron S., Zhang E., Peil L., Dudnakova T., Petfalski E., Granneman S., Rappsilber J., Tollervey D. d.tollervey@ed.ac.uk 21-10-2015: Contacted by IntAct-Help. Only protein-protein interactions yeast Saccharomyces cerevisiae Baker's yeast crac crac CRAC cross-linking and analysis of cDNAs crac partial nucleotide sequence partial nucleotide sequence identification partial nucleotide sequence Turowski TW., Lebaron S., Zhang E., Peil L., Dudnakova T., Petfalski E., Granneman S., Rappsilber J., Tollervey D. d.tollervey@ed.ac.uk Accepted 2015-SEP-04 by ORCHARD Nucleic acids research 2014 imex curation It is named in the text but I have deleted it; Fine, now only the aff chr exp is reported Rio1 mediates ATP-dependent final maturation of 40S ribosomal subunits. Rio1 mediates ATP-dependent final maturation of 40S ribosomal subunits. Nucleic acids research 2014 imex curation Turowski TW., Lebaron S., Zhang E., Peil L., Dudnakova T., Petfalski E., Granneman S., Rappsilber J., Tollervey D. d.tollervey@ed.ac.uk 21-10-2015: Contacted by IntAct-Help. Only protein-protein interactions yeast Saccharomyces cerevisiae Baker's yeast chromatography chromatography technology column chromatography chromatography de novo protein sequence de novo protein sequencing by mass spectrometry de novo protein sequence Turowski TW., Lebaron S., Zhang E., Peil L., Dudnakova T., Petfalski E., Granneman S., Rappsilber J., Tollervey D. d.tollervey@ed.ac.uk Accepted 2015-SEP-04 by ORCHARD Nucleic acids research 2014 imex curation It is named in the text but I have deleted it; Fine, now only the aff chr exp is reported Rio1 mediates ATP-dependent final maturation of 40S ribosomal subunits. Rio1 mediates ATP-dependent final maturation of 40S ribosomal subunits. Nucleic acids research 2014 imex curation Turowski TW., Lebaron S., Zhang E., Peil L., Dudnakova T., Petfalski E., Granneman S., Rappsilber J., Tollervey D. d.tollervey@ed.ac.uk 21-10-2015: Contacted by IntAct-Help. Only protein-protein interactions yeast Saccharomyces cerevisiae Baker's yeast affinity chrom affinity chromatography technology Affinity purification Affinity purification affinity chrom primer extension assay primer extension assay primer extension assay Turowski TW., Lebaron S., Zhang E., Peil L., Dudnakova T., Petfalski E., Granneman S., Rappsilber J., Tollervey D. d.tollervey@ed.ac.uk Accepted 2015-SEP-04 by ORCHARD Nucleic acids research 2014 imex curation It is named in the text but I have deleted it; Fine, now only the aff chr exp is reported dim1_yeast Dimethyladenosine transferase YPL266W DIM1 S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase 18S rRNA dimethylase 18S rRNA (adenine(1779)-N(6)/adenine(1780)-N(6))-dimethyltransferase protein protein yeast Saccharomyces cerevisiae Baker's yeast MGKAAKKKYSGATSSKQVSAEKHLSSVFKFNTDLGQHILKNPLVAQGIVDKAQIRPSDVVLEVGPGTGNLTVRILEQAKNVVAVEMDPRMAAELTKRVRGTPVEKKLEIMLGDFMKTELPYFDICISNTPYQISSPLVFKLINQPRPPRVSILMFQREFALRLLARPGDSLYCRLSANVQMWANVTHIMKVGKNNFRPPPQVESSVVRLEIKNPRPQVDYNEWDGLLRIVFVRKNRTISAGFKSTTVMDILEKNYKTFLAMNNEMVDDTKGSMHDVVKEKIDTVLKETDLGDKRAGKCDQNDFLRLLYAFHQVGIHFS EE77C9DF574A042A EE77C9DF574A042A enp1_yeast Essential nuclear protein 1 ENP1 MEG1 YBR247C YBR1635 protein protein yeast Saccharomyces cerevisiae Baker's yeast MARASSTKARKQRHDPLLKDLDAAQGTLKKINKKKLAQNDAANHDAANEEDGYIDSKASRKILQLAKEQQDEIEGEELAESERNKQFEARFTTMSYDDEDEDEDEDEEAFGEDISDFEPEGDYKEEEEIVEIDEEDAAMFEQYFKKSDDFNSLSGSYNLADKIMASIREKESQVEDMQDDEPLANEQNTSRGNISSGLKSGEGVALPEKVIKAYTTVGSILKTWTHGKLPKLFKVIPSLRNWQDVIYVTNPEEWSPHVVYEATKLFVSNLTAKESQKFINLILLERFRDNIETSEDHSLNYHIYRAVKKSLYKPSAFFKGFLFPLVETGCNVREATIAGSVLAKVSVPALHSSAALSYLLRLPFSPPTTVFIKILLDKKYALPYQTVDDCVYYFMRFRILDDGSNGEDATRVLPVIWHKAFLTFAQRYKNDITQDQRDFLLETVRQRGHKDIGPEIRRELLAGASREFVDPQEANDDLMIDVN 399A415131294637 399A415131294637 prp43_yeast Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP43 PRP43 Helicase JA1 YGL120C protein protein yeast Saccharomyces cerevisiae Baker's yeast MGSKRRFSSEHPDPVETSIPEQAAEIAEELSKQHPLPSEEPLVHHDAGEFKGLQRHHTSAEEAQKLEDGKINPFTGREFTPKYVDILKIRRELPVHAQRDEFLKLYQNNQIMVFVGETGSGKTTQIPQFVLFDEMPHLENTQVACTQPRRVAAMSVAQRVAEEMDVKLGEEVGYSIRFENKTSNKTILKYMTDGMLLREAMEDHDLSRYSCIILDEAHERTLATDILMGLLKQVVKRRPDLKIIIMSATLDAEKFQRYFNDAPLLAVPGRTYPVELYYTPEFQRDYLDSAIRTVLQIHATEEAGDILLFLTGEDEIEDAVRKISLEGDQLVREEGCGPLSVYPLYGSLPPHQQQRIFEPAPESHNGRPGRKVVISTNIAETSLTIDGIVYVVDPGFSKQKVYNPRIRVESLLVSPISKASAQQRAGRAGRTRPGKCFRLYTEEAFQKELIEQSYPEILRSNLSSTVLELKKLGIDDLVHFDFMDPPAPETMMRALEELNYLACLDDEGNLTPLGRLASQFPLDPMLAVMLIGSFEFQCSQEILTIVAMLSVPNVFIRPTKDKKRADDAKNIFAHPDGDHITLLNVYHAFKSDEAYEYGIHKWCRDHYLNYRSLSAADNIRSQLERLMNRYNLELNTTDYESPKYFDNIRKALASGFFMQVAKKRSGAKGYITVKDNQDVLIHPSTVLGHDAEWVIYNEFVLTSKNYIRTVTSVRPEWLIEIAPAYYDLSNFQKGDVKLSLERIKEKVDRLNELKQGKNKKKSKHSKK DA8A085EC50FF430 DA8A085EC50FF430 nob1_yeast 20S-pre-rRNA D-site endonuclease NOB1 Pre-rRNA-processing endonuclease NOB1 NIN1-binding protein NOB1 YOR29-07 YOR056C protein protein yeast Saccharomyces cerevisiae Baker's yeast MTENQTAHVRALILDATPLITQSYTHYQNYAQSFYTTPTVFQEIKDAQARKNLEIWQSLGTLKLVHPSENSIAKVSTFAKLTGDYSVLSANDLHILALTYELEIKLNNGDWRLRKKPGDALDASKADVGTDGKQKLTEDNKKEEDSESVPKKKNKRRGGKKQKAKREAREAREAENANLELESKAEEHVEEAGSKEQICNDENIKESSDLNEVFEDADDDGDWITPENLTEAIIKDSGEDTTGSLGVEASEEDRHVALNRPENQVALATGDFAVQNVALQMNLNLMNFMSGLKIKRIRNYMLRCHACFKIFPLPKDGKPKHFCASCGGQGTLLRCAVSVDSRTGNVTPHLKSNFQWNNRGNRYSVASPLSKNSQKRYGKKGHVHSKPQENVILREDQKEYEKVIKQEEWTRRHNEKILNNWIGGGSADNYISPFAITGLKQHNVRIGKGRYVNSSKRRS 96DEB818BB490A98 96DEB818BB490A98 rio2_yeast Serine/threonine-protein kinase RIO2 N1342 YNL207W RIO2 protein protein yeast Saccharomyces cerevisiae Baker's yeast MKLDTSHMRYLTTDDFRVLQAVEQGSRSHEVVPTPLIHQISGMRSQSGTNRAISDLAKLSLISKMRNVKYDGYRLTYNGIDYLALKTMLNRDTVYSVGNTIGVGKESDIYKVSDKNGNPRVMKIHRLGRTSFHSVRNNRDYLKKSNQGANWMHLSRLAANKEYQFMSMLYSKGFKVPEPFDNSRHIVVMELIEGYPMRRLRKHKNIPKLYSDLMCFIVDLANSGLIHCDFNEFNIMIKDKLEDENDCGFVVIDFPQCISIQHQDADYYFQRDVDCIRRFFKKKLKYEPKPDSSMLDTEGFGDGYKYAYPDFKRDVKRTDNLDELVQASGFSKKHPGDRGLETAVESMRNAVYNSDDDMSNDEAEEENGEGDYSEEDEYYDSELDNESSEDDSEDAQEEENERIIEALSSGVENLKMDKLGNYILE 02117D0328118E8D 02117D0328118E8D ltv1_yeast Protein LTV1 LTV1 YKL143W YKL2 Low-temperature viability protein 1 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSKKFSSKNSQRYVVVHRPHDDPSFYDTDASAHVLVPVSNPNKTSPEADLRKKDVSSTKPKGRRAHVGEAALYGINFDDSEYDYTQHLKPIGLDPENSIFIASKGNEQKVEKKNIEDLFIEPKYRRDEIEKDDALPVFQRGMAKPEYLLHQQDTTDEIRGFKPDMNPALREVLEALEDEAYVVNDDVVVEDISKKTQLQGDNYGEEEKEDDIFAQLLGSGEAKDEDEFEDEFDEWDIDNVENFEDENYVKEMAQFDNIENLEDLENIDYQADVRRFQKDNSILEKHNSDDEFSNAGLDSVNPSEEEDVLGELPSIQDKSKTGKKKRKSRQKKGAMSDVSGFSMSSSAIARTETMTVLDDQYDQIINGYENYEEELEEDEEQNYQPFDMSAERSDFESMLDDFLDNYELESGGRKLAKKDKEIERLKEAADEVSKGKLSQRRNRERQEKKKLEKVTNTLSSLKF F432C239B3B0A68A F432C239B3B0A68A rl24a_yeast 60S ribosomal protein L24-A RPL24A RP29 RPL30A YGL031C L30 YL21 Large ribosomal subunit protein eL24-A protein protein yeast Saccharomyces cerevisiae Baker's yeast MKVEIDSFSGAKIYPGRGTLFVRGDSKIFRFQNSKSASLFKQRKNPRRIAWTVLFRKHHKKGITEEVAKKRSRKTVKAQRPITGASLDLIKERRSLKPEVRKANREEKLKANKEKKKAEKAARKAEKAKSAGTQSSKFSKQQAKGAFQKVAATSR 9A79C4E409515716 rrna 20s precursor ribosomal RNA 20S yeast rrna ribosomal rna rRNA rrna yeast Saccharomyces cerevisiae Baker's yeast UAUCUGGUUGAUCCUGCCAGUAGUCAUAUGCUUGUCUCAAAGAUUAAGCCAUGCAUGUCUAAGUAUAAGCAAUUUAUACAGUGAAACUGCGAAUGGCUCAUUAAAUCAGUUAUCGUUUAUUUGAUAGUUCCUUUACUACAUGGUAUAACUGUGGUAAUUCUAGAGCUAAUACAUGCUUAAAAUCUCGACCCUUUGGAAGAGAUGUAUUUAUUAGAUAAAAAAUCAAUGUCUUCGGACUCUUUGAUGAUUCAUAAUAACUUUUCGAAUCGCAUGGCCUUGUGCUGGCGAUGGUUCAUUCAAAUUUCUGCCCUAUCAACUUUCGAUGGUAGGAUAGUGGCCUACCAUGGUUUCAACGGGUAACGGGGAAUAAGGGUUCGAUUCCGGAGAGGGAGCCUGAGAAACGGCUACCACAUCCAAGGAAGGCAGCAGGCGCGCAAAUUACCCAAUCCUAAUUCAGGGAGGUAGUGACAAUAAAUAACGAUACAGGGCCCAUUCGGGUCUUGUAAUUGGAAUGAGUACAAUGUAAAUACCUUAACGAGGAACAAUUGGAGGGCAAGUCUGGUGCCAGCAGCCGCGGUAAUUCCAGCUCCAAUAGCGUAUAUUAAAGUUGUUGCAGUUAAAAAGCUCGUAGUUGAACUUUGGGCCCGGUUGGCCGGUCCGAUUUUUUCGUGUACUGGAUUUCCAACGGGGCCUUUCCUUCUGGCUAACCUUGAGUCCUUGUGGCUCUUGGCGAACCAGGACUUUUACUUUGAAAAAAUUAGAGUGUUCAAAGCAGGCGUAUUGCUCGAAUAUAUUAGCAUGGAAUAAUAGAAUAGGACGUUUGGUUCUAUUUUGUUGGUUUCUAGGACCAUCGUAAUGAUUAAUAGGGACGGUCGGGGGCAUCAGUAUUCAAUUGUCAGAGGUGAAAUUCUUGGAUUUAUUGAAGACUAACUACUGCGAAAGCAUUUGCCAAGGACGUUUUCAUUAAUCAAGAACGAAAGUUAGGGGAUCGAAGAUGAUCAGAUACCGUCGUAGUCUUAACCAUAAACUAUGCCGACUAGGGAUCGGGUGGUGUUUUUUUAAUGACCCACUCGGCACCUUACGAGAAAUCAAAGUCUUUGGGUUCUGGGGGGAGUAUGGUCGCAAGGCUGAAACUUAAAGGAAUUGACGGAAGGGCACCACCAGGAGUGGAGCCUGCGGCUUAAUUUGACUCAACACGGGGAAACUCACCAGGUCCAGACACAAUAAGGAUUGACAGAUUGAGAGCUCUUUCUUGAUUUUGUGGGUGGUGGUGCAUGGCCGUUCUUAGUUGGUGGAGUGAUUUGUCUGCUUAAUUGCGAUAACGAACGAGACCUUAACCUACUAAAUAGUGGUGCUAGCAUUUGCUGGUUAUCCACUUCUUAGAGGGACUAUCGGUUUCAAGCCGAUGGAAGUUUGAGGCAAUAACAGGUCUGUGAUGCCCUUAGACGUUCUGGGCCGCACGCGCGCUACACUGACGGAGCCAGCGAGUCUAACCUUGGCCGAGAGGUCUUGGUAAUCUUGUGAAACUCCGUCGUGCUGGGGAUAGAGCAUUGUAAUUAUUGCUCUUCAACGAGGAAUUCCUAGUAAGCGCAAGUCAUCAGCUUGCGUUGAUUACGUCCCUGCCCUUUGUACACACCGCCCGUCGCUAGUACCGAUUGAAUGGCUUAGUGAGGCCUCAGGAUCUGCUUAGAGAAGGGGGCAACUCCAUCUCAGAGCGGAGAAUUUGGACAAACUUGGUCAUUUAGAGGAACUAAAAGUCGUAACAAGGUUUCCGUAGGUGAACCUGCGGAAGGAUCAUUAAAGAAAUUUAAUAAUUUUGAAAAUGGAUUUUUUUGUUUUGGCAAGAGCAUGAGAGCUUUUACUGGGCAAGAAGACAAGAGAUGGAGAGUCCAGCCGGGCCUGCGCUUAAGUGCGCGGUCUUGCUAGGCUUGUAAGUUUCUUUCUUGCUAUUCCAAACGGUGAGAGAUUUCUGUGCUUUUGUUAUAGGACAAUUAAAACCGUUUCAAUACAAC tsr1_yeast Ribosome biogenesis protein TSR1 TSR1 20S rRNA accumulation protein 1 YDL060W protein protein yeast Saccharomyces cerevisiae Baker's yeast MAGHSHRSSLKNGHKSYKSKHASKGALKRLYKGKVEKEPVGTGKPDKQVSKLQRKNKAKQLRAQRILDSIENRKLFEGKNGAAKIITIVPLVNDLDPLDILYKLLKCADDEGIMVQEVDSKRIFNVHIKKFKSNLKIIIPDMTNFLNILDCAKVADFVVFGLSGVQEVDEEFGEQIIRALELQGIASYIGVISNLSAVHEKEKFQLDVKQSLESYFKHFFPSEERVYNLEKNSDALNVLRTLCQRLPRSINWRDNRGYVVADFVDFVETSPDSGDLVIEGTVRGIGFNANRLVHIPDFGDFQLNKIEKISESSQKRKIIKEKATDSLSLELDLQTVFESNMNRDTLDEYAPEGTEDWSDYDEDFEYDGLTTARYDDHGFLPGREQTSKKAAVPKGTSDYQAKWYLDDVIDANEEEEAEQTNGKDETMMEIDDEMMVEQDNEEVAGDEEYDIEDNEGFEELSPEEEERQLREFRDMEKEDREFPDEIELEPSESAIERLKRYRGLKNLYNCDWQVDEKDPSSPAEWKRLLRIGNYKNTKNRIIKETKNEAQAIAGDRIRMFIRFPKFLLEKIQDPKQLLFAVYGLLLHEHKNAVVNFSLQRWEQYDKPVPSQEPIVVQYGVRRYTIQPLFSQGSNSPNNVHKYERFLHPDTVSVATCIAPVDFTQSPAIFFKPSPTDAKNIELIGHGTFLNADHSRILAKRAILTGHPFRFHKTVVTVRYMFFRPEDVEWFKSIPLFTKSGRSGFIKESLGTHGYFKATFDGKLSAQDVVAMSLYKRMWPMPSLPWNGM 71BA5461E8A858C7 71BA5461E8A858C7 rs14a_yeast 40S ribosomal protein S14-A RPS14A CRY1 RPL59 RP59A Small ribosomal subunit protein uS11-A YCR31C YCR031C protein protein yeast Saccharomyces cerevisiae Baker's yeast MSNVVQARDNSQVFGVARIYASFNDTFVHVTDLSGKETIARVTGGMKVKADRDESSPYAAMLAAQDVAAKCKEVGITAVHVKIRATGGTRTKTPGPGGQAALRALARSGLRIGRIEDVTPVPSDSTRKKGGRRGRRL 65A9212E10340A95 pno1_yeast Pre-rRNA-processing protein PNO1 PNO1 DIM2 Partner of NOB1 Ribosomal RNA-processing protein 20 RRP20 O3513 YOR3513C YOR145C protein protein yeast Saccharomyces cerevisiae Baker's yeast MVAPTALKKATVTPVSGQDGGSSRIIGINNTESIDEDDDDDVLLDDSDNNTAKEEVEGEEGSRKTHESKTVVVDDQGKPRFTSASKTQGNKIKFESRKIMVPPHRMTPLRNSWTKIYPPLVEHLKLQVRMNLKTKSVELRTNPKFTTDPGALQKGADFIKAFTLGFDLDDSIALLRLDDLYIETFEVKDVKTLTGDHLSRAIGRIAGKDGKTKFAIENATRTRIVLADSKIHILGGFTHIRMARESVVSLILGSPPGKVYGNLRTVASRLKERY F61E17CAB85BF60C F61E17CAB85BF60C rs4a_yeast 40S ribosomal protein S4-A RPS4A RP5 RPS7B S7 YS6 J2186 YJR145C Small ribosomal subunit protein eS4-A protein protein yeast Saccharomyces cerevisiae Baker's yeast MARGPKKHLKRLAAPHHWLLDKLSGCYAPRPSAGPHKLRESLPLIVFLRNRLKYALNGREVKAILMQRHVKVDGKVRTDTTYPAGFMDVITLDATNENFRLVYDVKGRFAVHRITDEEASYKLGKVKKVQLGKKGVPYVVTHDGRTIRYPDPNIKVNDTVKIDLASGKITDFIKFDAGKLVYVTGGRNLGRIGTIVHKERHDGGFDLVHIKDSLDNTFVTRLNNVFVIGEQGKPYISLPKGKGIKLSIAEERDRRRAQQGL mint F109594560F34734 rio1_yeast Serine/threonine-protein kinase RIO1 RIO1 RRP10 O3266 YOR3266C YOR119C Ribosomal RNA-processing protein 10 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSLEDKFDSLSVSQGASDHINNQLLEKYSHKIKTDELSFSRAKTSKDKANRATVENVLDPRTMRFLKSMVTRGVIADLNGCLSTGKEANVYHAFAGTGKAPVIDEETGQYEVLETDGSRAEYAIKIYKTSILVFKDRERYVDGEFRFRNSRSQHNPRKMIKIWAEKEFRNLKRIYQSGVIPAPKPIEVKNNVLVMEFLSRGNGFASPKLKDYPYKNRDEIFHYYHTMVAYMRLLYQVCRLVHADLSEYNTIVHDDKLYMIDVSQSVEPEHPMSLDFLRMDIKNVNLYFEKMGISIFPERVIFQFVISETLEKFKGDYNNISALVAYIASNLPIKSTEQDEAEDEIFRSLHLVRSLGGLEERDFDRYTDGKFDLLKSLIAHDNERNFAASEQFEFDNADHECSSGTEEFSDDEEDGSSGSEEDDEEEGEYYDDDEPKVLKGKKHEDKDLKKLRKQEAKDAKREKRKTKVKKHIKKKLVKKTKSKK 7051A55BFD37D0C0 rrna 18s yeast Ribosomal RNA 18S Yeast rrna ribosomal rna rRNA rrna yeast Saccharomyces cerevisiae Baker's yeast UAUCUGGUUGAUCCUGCCAGUAGUCAUAUGCUUGUCUCAAAGAUUAAGCCAUGCAUGUCUAAGUAUAAGCAAUUUAUACAGUGAAACUGCGAAUGGCUCAUUAAAUCAGUUAUCGUUUAUUUGAUAGUUCCUUUACUACAUGGUAUAACUGUGGUAAUUCUAGAGCUAAUACAUGCUUAAAAUCUCGACCCUUUGGAAGAGAUGUAUUUAUUAGAUAAAAAAUCAAUGUCUUCGGACUCUUUGAUGAUUCAUAAUAACUUUUCGAAUCGCAUGGCCUUGUGCUGGCGAUGGUUCAUUCAAAUUUCUGCCCUAUCAACUUUCGAUGGUAGGAUAGUGGCCUACCAUGGUUUCAACGGGUAACGGGGAAUAAGGGUUCGAUUCCGGAGAGGGAGCCUGAGAAACGGCUACCACAUCCAAGGAAGGCAGCAGGCGCGCAAAUUACCCAAUCCUAAUUCAGGGAGGUAGUGACAAUAAAUAACGAUACAGGGCCCAUUCGGGUCUUGUAAUUGGAAUGAGUACAAUGUAAAUACCUUAACGAGGAACAAUUGGAGGGCAAGUCUGGUGCCAGCAGCCGCGGUAAUUCCAGCUCCAAUAGCGUAUAUUAAAGUUGUUGCAGUUAAAAAGCUCGUAGUUGAACUUUGGGCCCGGUUGGCCGGUCCGAUUUUUUCGUGUACUGGAUUUCCAACGGGGCCUUUCCUUCUGGCUAACCUUGAGUCCUUGUGGCUCUUGGCGAACCAGGACUUUUACUUUGAAAAAAUUAGAGUGUUCAAAGCAGGCGUAUUGCUCGAAUAUAUUAGCAUGGAAUAAUAGAAUAGGACGUUUGGUUCUAUUUUGUUGGUUUCUAGGACCAUCGUAAUGAUUAAUAGGGACGGUCGGGGGCAUCAGUAUUCAAUUGUCAGAGGUGAAAUUCUUGGAUUUAUUGAAGACUAACUACUGCGAAAGCAUUUGCCAAGGACGUUUUCAUUAAUCAAGAACGAAAGUUAGGGGAUCGAAGAUGAUCAGAUACCGUCGUAGUCUUAACCAUAAACUAUGCCGACUAGGGAUCGGGUGGUGUUUUUUUAAUGACCCACUCGGCACCUUACGAGAAAUCAAAGUCUUUGGGUUCUGGGGGGAGUAUGGUCGCAAGGCUGAAACUUAAAGGAAUUGACGGAAGGGCACCACCAGGAGUGGAGCCUGCGGCUUAAUUUGACUCAACACGGGGAAACUCACCAGGUCCAGACACAAUAAGGAUUGACAGAUUGAGAGCUCUUUCUUGAUUUUGUGGGUGGUGGUGCAUGGCCGUUCUUAGUUGGUGGAGUGAUUUGUCUGCUUAAUUGCGAUAACGAACGAGACCUUAACCUACUAAAUAGUGGUGCUAGCAUUUGCUGGUUAUCCACUUCUUAGAGGGACUAUCGGUUUCAAGCCGAUGGAAGUUUGAGGCAAUAACAGGUCUGUGAUGCCCUUAGACGUUCUGGGCCGCACGCGCGCUACACUGACGGAGCCAGCGAGUCUAACCUUGGCCGAGAGGUCUUGGUAAUCUUGUGAAACUCCGUCGUGCUGGGGAUAGAGCAUUGUAAUUAUUGCUCUUCAACGAGGAAUUCCUAGUAAGCGCAAGUCAUCAGCUUGCGUUGAUUACGUCCCUGCCCUUUGUACACACCGCCCGUCGCUAGUACCGAUUGAAUGGCUUAGUGAGGCCUCAGGAUCUGCUUAGAGAAGGGGGCAACUCCAUCUCAGAGCGGAGAAUUUGGACAAACUUGGUCAUUUAGAGGAACUAAAAGUCGUAACAAGGUUUCCGUAGGUGAACCUGCGGAAGGAUCAUUA hrr25_yeast Casein kinase I homolog HRR25 YPL204W HRR25 protein protein yeast Saccharomyces cerevisiae Baker's yeast MDLRVGRKFRIGRKIGSGSFGDIYHGTNLISGEEVAIKLESIRSRHPQLDYESRVYRYLSGGVGIPFIRWFGREGEYNAMVIDLLGPSLEDLFNYCHRRFSFKTVIMLALQMFCRIQYIHGRSFIHRDIKPDNFLMGVGRRGSTVHVIDFGLSKKYRDFNTHRHIPYRENKSLTGTARYASVNTHLGIEQSRRDDLESLGYVLIYFCKGSLPWQGLKATTKKQKYDRIMEKKLNVSVETLCSGLPLEFQEYMAYCKNLKFDEKPDYLFLARLFKDLSIKLEYHNDHLFDWTMLRYTKAMVEKQRDLLIEKGDLNANSNAASASNSTDNKSETFNKIKLLAMKKFPTHFHYYKNEDKHNPSPEEIKQQTILNNNAASSLPEELLNALDKGMENLRQQQPQQQVQSSQPQPQPQQLQQQPNGQRPNYYPEPLLQQQQRDSQEQQQQVPMATTRATQYPPQINSNNFNTNQASVPPQMRSNPQQPPQDKPAGQSIWL C4D3107A470E8BAF C4D3107A470E8BAF nob1_20s-1 3 11 unspecified role unspecified role prey prey 7 unspecified role unspecified role bait bait HTP His Tev proteinA tag tandem tag tandem tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range physical association physical association Fig1A rio2_20s-1 3 11 unspecified role unspecified role prey prey 8 unspecified role unspecified role bait bait tag tap tagged calmodulin binding peptide plus protein a tag CBP-ProtA tagged CBP-ProtA tagged tap tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range physical association physical association Fig1A pno1_20s-1 3 11 unspecified role unspecified role prey prey 14 unspecified role unspecified role bait bait tag tap tagged calmodulin binding peptide plus protein a tag CBP-ProtA tagged CBP-ProtA tagged tap tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range physical association physical association Fig1A,B ltv1_20s-1 3 11 unspecified role unspecified role prey prey 9 unspecified role unspecified role bait bait HTP tag His Tev proteinA tag tandem tag tandem tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range physical association physical association Fig1A enp1_20s-1 3 11 unspecified role unspecified role prey prey 5 unspecified role unspecified role bait bait HTP tag His Tev proteinA tag tandem tag tandem tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range physical association physical association Fig1A rio1_20s-1 3 11 unspecified role unspecified role prey prey 16 unspecified role unspecified role bait bait Tag tap tagged calmodulin binding peptide plus protein a tag CBP-ProtA tagged CBP-ProtA tagged tap tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range physical association physical association Fig1, 2 dim1_20s-1 3 11 unspecified role unspecified role prey prey 4 unspecified role unspecified role bait bait HTP tag His Tev proteinA tag tandem tag tandem tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range physical association physical association Fig1A prp43_20s-1 3 11 unspecified role unspecified role prey prey 6 unspecified role unspecified role bait bait tag tap tagged calmodulin binding peptide plus protein a tag CBP-ProtA tagged CBP-ProtA tagged tap tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range physical association physical association Fig1A tsr1_20s-1 3 11 unspecified role unspecified role prey prey 12 unspecified role unspecified role bait bait Tag tap tagged calmodulin binding peptide plus protein a tag CBP-ProtA tagged CBP-ProtA tagged tap tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range physical association physical association Fig1A nob1-dim1-1 2 15 unspecified role unspecified role prey prey 11 rt-pcr reverse transcription pcr RT-PCR de novo protein sequence de novo protein sequencing by mass spectrometry de novo protein sequence unspecified role unspecified role prey prey 17 rt-pcr reverse transcription pcr RT-PCR de novo protein sequence de novo protein sequencing by mass spectrometry de novo protein sequence unspecified role unspecified role prey prey 10 unspecified role unspecified role prey prey 13 unspecified role unspecified role prey prey 12 unspecified role unspecified role prey prey 9 unspecified role unspecified role prey prey 5 unspecified role unspecified role prey prey 4 unspecified role unspecified role prey prey 18 unspecified role unspecified role prey prey 8 unspecified role unspecified role prey prey 7 unspecified role unspecified role bait bait HTP tag His Tev proteinA tag tandem tag tandem tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 14 unspecified role unspecified role prey prey association association Fig4, suppl fig 2C pno1-dim1-1 2 15 unspecified role unspecified role prey prey 17 rt-pcr reverse transcription pcr RT-PCR de novo protein sequence de novo protein sequencing by mass spectrometry de novo protein sequence unspecified role unspecified role prey prey 11 rt-pcr reverse transcription pcr RT-PCR de novo protein sequence de novo protein sequencing by mass spectrometry de novo protein sequence unspecified role unspecified role prey prey 13 unspecified role unspecified role prey prey 12 unspecified role unspecified role prey prey 9 unspecified role unspecified role prey prey 5 unspecified role unspecified role prey prey 4 unspecified role unspecified role prey prey 8 unspecified role unspecified role prey prey 7 unspecified role unspecified role prey prey 14 unspecified role unspecified role bait bait tag tandem tag tandem tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 16 unspecified role unspecified role prey prey association association Fig4, Suppl fig 2C rio1-1 2 15 unspecified role unspecified role prey prey 11 rt-pcr reverse transcription pcr RT-PCR de novo protein sequence de novo protein sequencing by mass spectrometry de novo protein sequence unspecified role unspecified role prey prey 10 unspecified role unspecified role prey prey 13 unspecified role unspecified role prey prey 7 unspecified role unspecified role prey prey 14 unspecified role unspecified role prey prey 16 unspecified role unspecified role bait bait Tag tap tagged calmodulin binding peptide plus protein a tag CBP-ProtA tagged CBP-ProtA tagged tap tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range association association Fig4 suppl fig 2C rio2-2 2 15 unspecified role unspecified role prey prey 11 unspecified role unspecified role prey prey 10 unspecified role unspecified role prey prey 13 unspecified role unspecified role prey prey 12 unspecified role unspecified role prey prey 9 unspecified role unspecified role prey prey 5 unspecified role unspecified role prey prey 4 unspecified role unspecified role prey prey 18 unspecified role unspecified role prey prey 8 unspecified role unspecified role bait bait tag tap tagged calmodulin binding peptide plus protein a tag CBP-ProtA tagged CBP-ProtA tagged tap tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range 7 unspecified role unspecified role prey prey 14 unspecified role unspecified role prey prey association association fig4 rio1-2 1 16 unspecified role unspecified role bait bait HTP His Tev proteinA tag tandem tag tandem tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 11 unspecified role unspecified role prey prey region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain The 20S rRNA is numbered as 18S nucleotides 1- 1800 and it has an additional 3' fragment (nucleotides 1800-2012) physical association physical association Fig5, Suppl Fig3A pno1-1 1 14 unspecified role unspecified role bait bait HTP tag His Tev proteinA tag tandem tag tandem tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 11 unspecified role unspecified role prey prey region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain The 20S rRNA is numbered as 18S nucleotides 1- 1800 and it has an additional 3' fragment (nucleotides 1800-2012) The 20S rRNA is numbered as 18S nucleotides 1- 1800 and it has an additional 3' fragment (nucleotides 1800-2012) physical association physical association Fig5, suppl fig3B nob1-1 1 7 unspecified role unspecified role bait bait HTP tag His Tev proteinA tag tandem tag tandem tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 11 unspecified role unspecified role prey prey region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain The 20S rRNA is numbered as 18S nucleotides 1- 1800 and it has an additional 3' fragment (nucleotides 1800-2012) physical association physical association Fig5, Suppl fig3B