IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ α-Synuclein interacts with the switch region of Rab8a in a Ser129 phosphorylation-dependent manner. α-Synuclein interacts with the switch region of Rab8a in a Ser129 phosphorylation-dependent manner. Neurobiology of disease 2014 imex curation Yin G., Lopes da Fonseca T., Eisbach SE., Anduaga AM., Breda C., Orcellet ML., Szegő ÉM., Guerreiro P., Lázaro DF., Braus GH., Fernandez CO., Griesinger C., Becker S., Goody RS., Itzen A., Giorgini F., Outeiro TF., Zweckstetter M. Parkinsons - Interactions investigated in the context of Parkinsons disease Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Results from Figure 6 and Supp 4 not included as the origin of GFP-Rab8A is not stated. markus.zweckstetter@dzne.de,tiago.outeiro@med.uni-goettingen.de 21-10-2015: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro nmr nuclear magnetic resonance NMR NMR nmr predetermined predetermined participant predetermined Yin G., Lopes da Fonseca T., Eisbach SE., Anduaga AM., Breda C., Orcellet ML., Szegő ÉM., Guerreiro P., Lázaro DF., Braus GH., Fernandez CO., Griesinger C., Becker S., Goody RS., Itzen A., Giorgini F., Outeiro TF., Zweckstetter M. Parkinsons - Interactions investigated in the context of Parkinsons disease Results from Figure 6 and Supp 4 not included as the origin of GFP-Rab8A is not stated. From the Bleimling reference, the his-tag and chaperone proteins have been removed. ( 15C radiolabeled Rab8A was co-expressed with chaperones GroEL and GroES.) Sequence mapping of Rab8a is given in relation to Uniprot P61006, rather than residues given here- notabley for "A173-G181" and " I193-T194". markus.zweckstetter@dzne.de,tiago.outeiro@med.uni-goettingen.de Accepted 2015-OCT-07 AT 09:28 BST AT 09:28 BST by ORCHARD Neurobiology of disease 2014 imex curation I have altered all the spectrally defined regions to "binding region" rather than "sufficient to bind" - I have done this throughout -hope this is OK ("Rab8a selectively interacted with the acidic C-terminus of αS (Figs. 2A, B" and fig 4); I have added back links to EBI-11175363 - but they keep on vanishing (for both interactions) I must have forgotten to remove the dye after cloning. α-Synuclein interacts with the switch region of Rab8a in a Ser129 phosphorylation-dependent manner. α-Synuclein interacts with the switch region of Rab8a in a Ser129 phosphorylation-dependent manner. Neurobiology of disease 2014 imex curation Yin G., Lopes da Fonseca T., Eisbach SE., Anduaga AM., Breda C., Orcellet ML., Szegő ÉM., Guerreiro P., Lázaro DF., Braus GH., Fernandez CO., Griesinger C., Becker S., Goody RS., Itzen A., Giorgini F., Outeiro TF., Zweckstetter M. Parkinsons - Interactions investigated in the context of Parkinsons disease Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Results from Figure 6 and Supp 4 not included as the origin of GFP-Rab8A is not stated. markus.zweckstetter@dzne.de,tiago.outeiro@med.uni-goettingen.de 21-10-2015: Contacted by IntAct-Help. Only protein-protein interactions human-h4 Homo sapiens neurogioma cell line h4 Human neuroglioma fluorescence imaging fluorescence microscopy fluorescence imaging immunocytochemistry immunocytochemistry Yin G., Lopes da Fonseca T., Eisbach SE., Anduaga AM., Breda C., Orcellet ML., Szegő ÉM., Guerreiro P., Lázaro DF., Braus GH., Fernandez CO., Griesinger C., Becker S., Goody RS., Itzen A., Giorgini F., Outeiro TF., Zweckstetter M. Parkinsons - Interactions investigated in the context of Parkinsons disease Results from Figure 6 and Supp 4 not included as the origin of GFP-Rab8A is not stated. markus.zweckstetter@dzne.de,tiago.outeiro@med.uni-goettingen.de Accepted 2015-OCT-07 AT 09:28 BST AT 09:28 BST by ORCHARD Neurobiology of disease 2014 imex curation I have altered all the spectrally defined regions to "binding region" rather than "sufficient to bind" - I have done this throughout -hope this is OK ("Rab8a selectively interacted with the acidic C-terminus of αS (Figs. 2A, B" and fig 4); I have added back links to EBI-11175363 - but they keep on vanishing (for both interactions) I must have forgotten to remove the dye after cloning. α-Synuclein interacts with the switch region of Rab8a in a Ser129 phosphorylation-dependent manner. α-Synuclein interacts with the switch region of Rab8a in a Ser129 phosphorylation-dependent manner. Neurobiology of disease 2014 imex curation Yin G., Lopes da Fonseca T., Eisbach SE., Anduaga AM., Breda C., Orcellet ML., Szegő ÉM., Guerreiro P., Lázaro DF., Braus GH., Fernandez CO., Griesinger C., Becker S., Goody RS., Itzen A., Giorgini F., Outeiro TF., Zweckstetter M. Parkinsons - Interactions investigated in the context of Parkinsons disease Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Results from Figure 6 and Supp 4 not included as the origin of GFP-Rab8A is not stated. markus.zweckstetter@dzne.de,tiago.outeiro@med.uni-goettingen.de 21-10-2015: Contacted by IntAct-Help. Only protein-protein interactions mouse-synaptosome Mouse synaptosome synaptosome Synaptosome anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Yin G., Lopes da Fonseca T., Eisbach SE., Anduaga AM., Breda C., Orcellet ML., Szegő ÉM., Guerreiro P., Lázaro DF., Braus GH., Fernandez CO., Griesinger C., Becker S., Goody RS., Itzen A., Giorgini F., Outeiro TF., Zweckstetter M. Parkinsons - Interactions investigated in the context of Parkinsons disease Results from Figure 6 and Supp 4 not included as the origin of GFP-Rab8A is not stated. mouse cortical synaptosomes markus.zweckstetter@dzne.de,tiago.outeiro@med.uni-goettingen.de Accepted 2015-OCT-07 AT 09:28 BST AT 09:28 BST by ORCHARD Neurobiology of disease 2014 imex curation I have altered all the spectrally defined regions to "binding region" rather than "sufficient to bind" - I have done this throughout -hope this is OK ("Rab8a selectively interacted with the acidic C-terminus of αS (Figs. 2A, B" and fig 4); I have added back links to EBI-11175363 - but they keep on vanishing (for both interactions) I must have forgotten to remove the dye after cloning. α-Synuclein interacts with the switch region of Rab8a in a Ser129 phosphorylation-dependent manner. α-Synuclein interacts with the switch region of Rab8a in a Ser129 phosphorylation-dependent manner. Neurobiology of disease 2014 imex curation Yin G., Lopes da Fonseca T., Eisbach SE., Anduaga AM., Breda C., Orcellet ML., Szegő ÉM., Guerreiro P., Lázaro DF., Braus GH., Fernandez CO., Griesinger C., Becker S., Goody RS., Itzen A., Giorgini F., Outeiro TF., Zweckstetter M. Parkinsons - Interactions investigated in the context of Parkinsons disease Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Results from Figure 6 and Supp 4 not included as the origin of GFP-Rab8A is not stated. markus.zweckstetter@dzne.de,tiago.outeiro@med.uni-goettingen.de 21-10-2015: Contacted by IntAct-Help. Only protein-protein interactions rat-hippocampus Rattus norvegicus hippocampal neurons hippocampus Hippocampus [Brain hippocampus] anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Yin G., Lopes da Fonseca T., Eisbach SE., Anduaga AM., Breda C., Orcellet ML., Szegő ÉM., Guerreiro P., Lázaro DF., Braus GH., Fernandez CO., Griesinger C., Becker S., Goody RS., Itzen A., Giorgini F., Outeiro TF., Zweckstetter M. Parkinsons - Interactions investigated in the context of Parkinsons disease Results from Figure 6 and Supp 4 not included as the origin of GFP-Rab8A is not stated. mouse cortical synaptosomes markus.zweckstetter@dzne.de,tiago.outeiro@med.uni-goettingen.de Accepted 2015-OCT-07 AT 09:28 BST AT 09:28 BST by ORCHARD Neurobiology of disease 2014 imex curation I have altered all the spectrally defined regions to "binding region" rather than "sufficient to bind" - I have done this throughout -hope this is OK ("Rab8a selectively interacted with the acidic C-terminus of αS (Figs. 2A, B" and fig 4); I have added back links to EBI-11175363 - but they keep on vanishing (for both interactions) I must have forgotten to remove the dye after cloning. α-Synuclein interacts with the switch region of Rab8a in a Ser129 phosphorylation-dependent manner. α-Synuclein interacts with the switch region of Rab8a in a Ser129 phosphorylation-dependent manner. Neurobiology of disease 2014 imex curation Yin G., Lopes da Fonseca T., Eisbach SE., Anduaga AM., Breda C., Orcellet ML., Szegő ÉM., Guerreiro P., Lázaro DF., Braus GH., Fernandez CO., Griesinger C., Becker S., Goody RS., Itzen A., Giorgini F., Outeiro TF., Zweckstetter M. Parkinsons - Interactions investigated in the context of Parkinsons disease Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Results from Figure 6 and Supp 4 not included as the origin of GFP-Rab8A is not stated. markus.zweckstetter@dzne.de,tiago.outeiro@med.uni-goettingen.de 21-10-2015: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro fluorescence spectr classical fluorescence spectroscopy fluorescence spectr predetermined predetermined participant predetermined Yin G., Lopes da Fonseca T., Eisbach SE., Anduaga AM., Breda C., Orcellet ML., Szegő ÉM., Guerreiro P., Lázaro DF., Braus GH., Fernandez CO., Griesinger C., Becker S., Goody RS., Itzen A., Giorgini F., Outeiro TF., Zweckstetter M. Parkinsons - Interactions investigated in the context of Parkinsons disease Results from Figure 6 and Supp 4 not included as the origin of GFP-Rab8A is not stated. ThT is added as a dye to visualize and quantify the presence or fibrilization of misfolded protein aggregates. markus.zweckstetter@dzne.de,tiago.outeiro@med.uni-goettingen.de Accepted 2015-OCT-07 AT 09:28 BST AT 09:28 BST by ORCHARD Neurobiology of disease 2014 imex curation I have altered all the spectrally defined regions to "binding region" rather than "sufficient to bind" - I have done this throughout -hope this is OK ("Rab8a selectively interacted with the acidic C-terminus of αS (Figs. 2A, B" and fig 4); I have added back links to EBI-11175363 - but they keep on vanishing (for both interactions) I must have forgotten to remove the dye after cloning. α-Synuclein interacts with the switch region of Rab8a in a Ser129 phosphorylation-dependent manner. α-Synuclein interacts with the switch region of Rab8a in a Ser129 phosphorylation-dependent manner. Neurobiology of disease 2014 imex curation Yin G., Lopes da Fonseca T., Eisbach SE., Anduaga AM., Breda C., Orcellet ML., Szegő ÉM., Guerreiro P., Lázaro DF., Braus GH., Fernandez CO., Griesinger C., Becker S., Goody RS., Itzen A., Giorgini F., Outeiro TF., Zweckstetter M. Parkinsons - Interactions investigated in the context of Parkinsons disease Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Results from Figure 6 and Supp 4 not included as the origin of GFP-Rab8A is not stated. markus.zweckstetter@dzne.de,tiago.outeiro@med.uni-goettingen.de 21-10-2015: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro tem transmission electron microscopy tem predetermined predetermined participant predetermined Yin G., Lopes da Fonseca T., Eisbach SE., Anduaga AM., Breda C., Orcellet ML., Szegő ÉM., Guerreiro P., Lázaro DF., Braus GH., Fernandez CO., Griesinger C., Becker S., Goody RS., Itzen A., Giorgini F., Outeiro TF., Zweckstetter M. Parkinsons - Interactions investigated in the context of Parkinsons disease Results from Figure 6 and Supp 4 not included as the origin of GFP-Rab8A is not stated. markus.zweckstetter@dzne.de,tiago.outeiro@med.uni-goettingen.de Accepted 2015-OCT-07 AT 09:28 BST AT 09:28 BST by ORCHARD Neurobiology of disease 2014 imex curation I have altered all the spectrally defined regions to "binding region" rather than "sufficient to bind" - I have done this throughout -hope this is OK ("Rab8a selectively interacted with the acidic C-terminus of αS (Figs. 2A, B" and fig 4); I have added back links to EBI-11175363 - but they keep on vanishing (for both interactions) I must have forgotten to remove the dye after cloning. rab8a_rat Ras-related protein Rab-8A Rab8a Rab8 protein protein rat Rattus norvegicus (Rat) Rat MAKTYDYLFKLLLIGDSGVGKTCVLFRFSEDAFNSTFISTIGIDFKIRTIELDGKRIKLQIWDTAGQERFRTITTAYYRGAMGIMLVYDITNEKSFDNIRNWIRNIEEHASADVEKMILGNKCDVNDKRQVSKERGEKLALDYGIKFMETSAKANINVENAFFTLARDIKAKMDKKLEGNSPQGSSHGVKITVEQQKRTSFFRCSLL AC89DC85588FB8F8 syua_mouse Alpha-synuclein Snca Syn Non-A beta component of AD amyloid Non-A4 component of amyloid precursor protein protein mouse Mus musculus Mouse MDVFMKGLSKAKEGVVAAAEKTKQGVAEAAGKTKEGVLYVGSKTKEGVVHGVTTVAEKTKEQVTNVGGAVVTGVTAVAQKTVEGAGNIAAATGFVKKDQMGKGEEGYPQEGILEDMPVDPGSEAYEMPSEEGYQDYEPEA 1FFD19D7E15E636C syua_rat Alpha-synuclein Snca protein protein rat Rattus norvegicus (Rat) Rat MDVFMKGLSKAKEGVVAAAEKTKQGVAEAAGKTKEGVLYVGSKTKEGVVHGVTTVAEKTKEQVTNVGGAVVTGVTAVAQKTVEGAGNIAAATGFVKKDQMGKGEEGYPQEGILEDMPVDPSSEAYEMPSEEGYQDYEPEA 1FFD19CD3B9E636C guanosine 5'-[beta,gamma-imido]triphosphate guanosine 5'-[beta,gamma-imido]triphosphate 5'-Guanylic acid, monoanhydride with imidodiphosphoric acid 5'-guanylylimidodiphosphate GMP-PNP GMP-Pnp Gpp(NH)-p Gpp(NH)p Guanylyl imidodiphosphate 5'-O-(hydroxy{[hydroxy(phosphonoamino)phosphoryl]oxy}phosphoryl)guanosine small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) rab8a_mouse Ras-related protein Rab-8A Rab8a Mel Rab8 Oncogene c-mel protein protein mouse Mus musculus Mouse MAKTYDYLFKLLLIGDSGVGKTCVLFRFSEDAFNSTFISTIGIDFKIRTIELDGKRIKLQIWDTAGQERFRTITTAYYRGAMGIMLVYDITNEKSFDNIRNWIRNIEEHASADVEKMILGNKCDVNDKRQVSKERGEKLALDYGIKFMETSAKANINVENAFFTLARDIKAKMDKKLEGNSPQGSSHGVKITVEQQKRTSFFRCSLL DIP protein P55258 original sequence version: 143 AC89DC85588FB8F8 gdp guanosine 5-diphosphate small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) rab8a_human Ras-related protein Rab-8A Oncogene c-mel RAB8A MEL RAB8 protein protein human Homo sapiens Human MAKTYDYLFKLLLIGDSGVGKTCVLFRFSEDAFNSTFISTIGIDFKIRTIELDGKRIKLQIWDTAGQERFRTITTAYYRGAMGIMLVYDITNEKSFDNIRNWIRNIEEHASADVEKMILGNKCDVNDKRQVSKERGEKLALDYGIKFMETSAKANINVENAFFTLARDIKAKMDKKLEGNSPQGSNQGVKITPDQQKRSSFFRCVLL DIP protein P61006 original sequence version: 82 DIP protein P61006 original sequence version: 100 DIP protein P61006 original sequence version: 128 DIP protein P61006 original sequence version: 132 AA52DBF54A2CD056 syua_human Alpha-synuclein Non-A beta component of AD amyloid Non-A4 component of amyloid precursor SNCA NACP PARK1 protein protein human Homo sapiens Human MDVFMKGLSKAKEGVVAAAEKTKQGVAEAAGKTKEGVLYVGSKTKEGVVHGVATVAEKTKEQVTNVGGAVVTGVTAVAQKTVEGAGSIAAATGFVKKDQLGKNEEGAPQEGILEDMPVDPDNEAYEMPSEEGYQDYEPEA DIP protein P37840 original sequence version: 124 DIP protein P37840 original sequence version: 175 DIP protein P37840 original sequence version: 178 6BB2F12128931663 snca-rab8a-1 3 11 unspecified role unspecified role prey prey 8 unspecified role unspecified role bait bait physical association physical association 1A snca-rab8a-2 3 11 unspecified role unspecified role bait bait 8 unspecified role unspecified role prey prey physical association physical association 1B snca-rab8a-3 4 7 unspecified role unspecified role prey prey 9 unspecified role unspecified role bait bait physical association physical association 1A rab8a-snca-1 1 12 cofactor cofactor coenzyme neutral component neutral component whole_molecule sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined chemical synthesis Chemical synthesis (Chemical synthesis) 13 unspecified role unspecified role neutral component neutral component n_terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range whole_molecule sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 14 unspecified role unspecified role neutral component neutral component undefined mutation mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain Wed Jul 06 16:41:57 BST 2016 Sequence change details about this feature cannot be ascertained or do not fit with the current version of the referenced protein, so they have been deleted as a result of our quality control procedures. The original label was 'ser129asp' region 15n label 15n label 15N N15 15N N15 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ser129 opser O-phospho-L-serine (2S)-2-amino-3-(phosphonooxy)propanoic acid 2-amino-3-hydroxypropanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)propanoic acid ACT_SITE Phosphoserine intermediate MOD_RES Phosphoserine O-phospho-L-serine O-phosphonoserine O-phosphorylated L-serine O3-phosphoserine serine phosphate ester OPSer Phospho Phospho Seryl Phosphorylation phosphorylations certain certain sequence position certain certain certain sequence position certain increasing-ptm ptm increasing an interaction increasing-ptm region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 26 29 direct interaction direct interaction 2A, 4, Supp 2B gdp-rab8a-1 1 10 cofactor cofactor coenzyme neutral component neutral component whole_molecule sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined chemical synthesis Chemical synthesis (Chemical synthesis) 13 unspecified role unspecified role neutral component neutral component n_terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range whole_molecule sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 14 unspecified role unspecified role neutral component neutral component region 15n label 15n label 15N N15 15N N15 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ser129 opser O-phospho-L-serine (2S)-2-amino-3-(phosphonooxy)propanoic acid 2-amino-3-hydroxypropanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)propanoic acid ACT_SITE Phosphoserine intermediate MOD_RES Phosphoserine O-phospho-L-serine O-phosphonoserine O-phosphorylated L-serine O3-phosphoserine serine phosphate ester OPSer Phospho Phospho Seryl Phosphorylation phosphorylations certain certain sequence position certain certain certain sequence position certain observed-ptm observed-ptm observed ptm observed-ptm region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 37 40 44 40 direct interaction direct interaction 2B, Supp 2B snca-snca-13 5 12 unspecified role unspecified role neutral component neutral component whole_molecule sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 13 stimulator stimulator neutral component neutral component n_terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range whole_molecule sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 14 unspecified role unspecified role neutral component neutral component whole_molecule sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 14 unspecified role unspecified role neutral component neutral component whole_molecule sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 50 47 52 54 physical association physical association 5A gdp-rab8a-2 6 12 unspecified role unspecified role neutral component neutral component whole_molecule sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 13 stimulator stimulator neutral component neutral component whole_molecule sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain n_terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) 14 unspecified role unspecified role neutral component neutral component whole_molecule sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 14 unspecified role unspecified role neutral component neutral component whole_molecule sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 57 59 64 62 physical association physical association 5B gdp-rab8a-3 1 12 cofactor cofactor coenzyme neutral component neutral component whole_molecule sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined chemical synthesis Chemical synthesis (Chemical synthesis) 13 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region 15n label 15n label 15N N15 15N N15 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 14 unspecified role unspecified role neutral component neutral component ser129 opser O-phospho-L-serine (2S)-2-amino-3-(phosphonooxy)propanoic acid 2-amino-3-hydroxypropanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)propanoic acid ACT_SITE Phosphoserine intermediate MOD_RES Phosphoserine O-phospho-L-serine O-phosphonoserine O-phosphorylated L-serine O3-phosphoserine serine phosphate ester OPSer Phospho Phospho Seryl Phosphorylation phosphorylations certain certain sequence position certain certain certain sequence position certain increasing-ptm ptm increasing an interaction increasing-ptm region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain chemical synthesis Chemical synthesis (Chemical synthesis) 69 67 69 73 direct interaction direct interaction Supp 2C gdp-rab8a-4 1 12 cofactor cofactor coenzyme neutral component neutral component whole_molecule sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined chemical synthesis Chemical synthesis (Chemical synthesis) 13 unspecified role unspecified role neutral component neutral component region 15n label 15n label 15N N15 15N N15 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined alpha2helix_switch_regions binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined c_terminal_region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain whole_molecule sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 14 unspecified role unspecified role neutral component neutral component undefined mutation mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain Wed Jul 06 16:39:14 BST 2016 Sequence change details about this feature cannot be ascertained or do not fit with the current version of the referenced protein, so they have been deleted as a result of our quality control procedures. The original label was 'ser129asp' ser129 opser O-phospho-L-serine (2S)-2-amino-3-(phosphonooxy)propanoic acid 2-amino-3-hydroxypropanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)propanoic acid ACT_SITE Phosphoserine intermediate MOD_RES Phosphoserine O-phospho-L-serine O-phosphonoserine O-phosphorylated L-serine O3-phosphoserine serine phosphate ester OPSer Phospho Phospho Seryl Phosphorylation phosphorylations certain certain sequence position certain certain certain sequence position certain increasing-ptm ptm increasing an interaction increasing-ptm region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 81 76 direct interaction direct interaction 3 4235rw3-1 2 14 unspecified role unspecified role neutral component neutral component 13 unspecified role unspecified role neutral component neutral component colocalization colocalization Supp 4A gdp-rab8a-5 5 14 unspecified role unspecified role neutral component neutral component whole_molecule sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 14 unspecified role unspecified role neutral component neutral component whole_molecule sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 93 91 physical association physical association 5Ai snca-snca-14 6 14 unspecified role unspecified role neutral component neutral component whole_molecule sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 14 unspecified role unspecified role neutral component neutral component whole_molecule sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 98 96 physical association physical association 5Bi