IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ The intermediate filament protein, vimentin, is a regulator of NOD2 activity. The intermediate filament protein, vimentin, is a regulator of NOD2 activity. Gut 2013 imex curation Stevens C., Henderson P., Nimmo ER., Soares DC., Dogan B., Simpson KW., Barrett JC., null null., Wilson DC., Satsangi J. Crohn's disease - Interactions of proteins identified as having a link to Crohn's disease IBD - Inflammatory bowel disease craig.stevens@ed.ac.uk 17-Sep-2015: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-sw480 Human SW480 adenocarcinoma cell line sw480 SW480- human primary colorectal adenocarcinoma cell line There is a G -> A mutation in codon 273 of the p53 gene resulting in an Arg -> His substitution and a C -> T mutation in codon 309 resulting in a Pro -> Ser substitution. http://www.atcc.org/ATCCAdvancedCatalogSearch/ProductDetails/tabid/452/Default.aspx?ATCCNum=CCL-228&Template=cellBiology anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Stevens C., Henderson P., Nimmo ER., Soares DC., Dogan B., Simpson KW., Barrett JC., null null., Wilson DC., Satsangi J. Crohn's disease - Interactions of proteins identified as having a link to Crohn's disease craig.stevens@ed.ac.uk Accepted 2015-JUL-13 by ORCHARD Gut 2013 imex curation The intermediate filament protein, vimentin, is a regulator of NOD2 activity. The intermediate filament protein, vimentin, is a regulator of NOD2 activity. Gut 2013 imex curation Stevens C., Henderson P., Nimmo ER., Soares DC., Dogan B., Simpson KW., Barrett JC., null null., Wilson DC., Satsangi J. Crohn's disease - Interactions of proteins identified as having a link to Crohn's disease IBD - Inflammatory bowel disease craig.stevens@ed.ac.uk 17-Sep-2015: Contacted by IntAct-Help. Only protein-protein interactions imex curation yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Stevens C., Henderson P., Nimmo ER., Soares DC., Dogan B., Simpson KW., Barrett JC., null null., Wilson DC., Satsangi J. Crohn's disease - Interactions of proteins identified as having a link to Crohn's disease craig.stevens@ed.ac.uk Accepted 2015-JUL-13 by ORCHARD Gut 2013 imex curation The intermediate filament protein, vimentin, is a regulator of NOD2 activity. The intermediate filament protein, vimentin, is a regulator of NOD2 activity. Gut 2013 imex curation Stevens C., Henderson P., Nimmo ER., Soares DC., Dogan B., Simpson KW., Barrett JC., null null., Wilson DC., Satsangi J. Crohn's disease - Interactions of proteins identified as having a link to Crohn's disease IBD - Inflammatory bowel disease craig.stevens@ed.ac.uk 17-Sep-2015: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. fluorescence imaging fluorescence microscopy fluorescence imaging immunocytochemistry immunocytochemistry Stevens C., Henderson P., Nimmo ER., Soares DC., Dogan B., Simpson KW., Barrett JC., null null., Wilson DC., Satsangi J. Crohn's disease - Interactions of proteins identified as having a link to Crohn's disease craig.stevens@ed.ac.uk Accepted 2015-JUL-13 by ORCHARD Gut 2013 Only protein-protein interactions imex curation imex curation The intermediate filament protein, vimentin, is a regulator of NOD2 activity. The intermediate filament protein, vimentin, is a regulator of NOD2 activity. Gut 2013 imex curation Stevens C., Henderson P., Nimmo ER., Soares DC., Dogan B., Simpson KW., Barrett JC., null null., Wilson DC., Satsangi J. Crohn's disease - Interactions of proteins identified as having a link to Crohn's disease IBD - Inflammatory bowel disease craig.stevens@ed.ac.uk 17-Sep-2015: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti bait coip anti bait coimmunoprecipitation anti bait coip anti tag western anti tag western blot anti tag western Stevens C., Henderson P., Nimmo ER., Soares DC., Dogan B., Simpson KW., Barrett JC., null null., Wilson DC., Satsangi J. Crohn's disease - Interactions of proteins identified as having a link to Crohn's disease craig.stevens@ed.ac.uk Accepted 2015-JUL-13 by ORCHARD Gut 2013 imex curation The intermediate filament protein, vimentin, is a regulator of NOD2 activity. The intermediate filament protein, vimentin, is a regulator of NOD2 activity. Gut 2013 imex curation Stevens C., Henderson P., Nimmo ER., Soares DC., Dogan B., Simpson KW., Barrett JC., null null., Wilson DC., Satsangi J. Crohn's disease - Interactions of proteins identified as having a link to Crohn's disease IBD - Inflammatory bowel disease craig.stevens@ed.ac.uk 17-Sep-2015: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-sw480 Human SW480 adenocarcinoma cell line sw480 SW480- human primary colorectal adenocarcinoma cell line There is a G -> A mutation in codon 273 of the p53 gene resulting in an Arg -> His substitution and a C -> T mutation in codon 309 resulting in a Pro -> Ser substitution. http://www.atcc.org/ATCCAdvancedCatalogSearch/ProductDetails/tabid/452/Default.aspx?ATCCNum=CCL-228&Template=cellBiology anti bait coip anti bait coimmunoprecipitation anti bait coip anti tag western anti tag western blot anti tag western Stevens C., Henderson P., Nimmo ER., Soares DC., Dogan B., Simpson KW., Barrett JC., null null., Wilson DC., Satsangi J. Crohn's disease - Interactions of proteins identified as having a link to Crohn's disease craig.stevens@ed.ac.uk Accepted 2015-JUL-13 by ORCHARD Gut 2013 imex curation vime_human Vimentin VIM protein protein human Homo sapiens Human MSTRSVSSSSYRRMFGGPGTASRPSSSRSYVTTSTRTYSLGSALRPSTSRSLYASSPGGVYATRSSAVRLRSSVPGVRLLQDSVDFSLADAINTEFKNTRTNEKVELQELNDRFANYIDKVRFLEQQNKILLAELEQLKGQGKSRLGDLYEEEMRELRRQVDQLTNDKARVEVERDNLAEDIMRLREKLQEEMLQREEAENTLQSFRQDVDNASLARLDLERKVESLQEEIAFLKKLHEEEIQELQAQIQEQHVQIDVDVSKPDLTAALRDVRQQYESVAAKNLQEAEEWYKSKFADLSEAANRNNDALRQAKQESTEYRRQVQSLTCEVDALKGTNESLERQMREMEENFAVEAANYQDTIGRLQDEIQNMKEEMARHLREYQDLLNVKMALDIEIATYRKLLEGEESRISLPLPNFSSLNLRETNLDSLPLVDTHSKRTLLIKTVETRDGQVINETSQHHDDLE DIP protein P08670 original sequence version: 195 DIP protein P08670 original sequence version: 209 DIP protein P08670 original sequence version: 192 DIP protein P08670 original sequence version: 175 BAB54026665B015A nod2_human Nucleotide-binding oligomerization domain-containing protein 2 IBD1 NOD2 CARD15 Caspase recruitment domain-containing protein 15 Inflammatory bowel disease protein 1 protein protein human Homo sapiens Human MGEEGGSASHDEEERASVLLGHSPGCEMCSQEAFQAQRSQLVELLVSGSLEGFESVLDWLLSWEVLSWEDYEGFHLLGQPLSHLARRLLDTVWNKGTWACQKLIAAAQEAQADSQSPKLHGCWDPHSLHPARDLQSHRPAIVRRLHSHVENMLDLAWERGFVSQYECDEIRLPIFTPSQRARRLLDLATVKANGLAAFLLQHVQELPVPLALPLEAATCKKYMAKLRTTVSAQSRFLSTYDGAETLCLEDIYTENVLEVWADVGMAGPPQKSPATLGLEELFSTPGHLNDDADTVLVVGEAGSGKSTLLQRLHLLWAAGQDFQEFLFVFPFSCRQLQCMAKPLSVRTLLFEHCCWPDVGQEDIFQLLLDHPDRVLLTFDGFDEFKFRFTDRERHCSPTDPTSVQTLLFNLLQGNLLKNARKVVTSRPAAVSAFLRKYIRTEFNLKGFSEQGIELYLRKRHHEPGVADRLIRLLQETSALHGLCHLPVFSWMVSKCHQELLLQEGGSPKTTTDMYLLILQHFLLHATPPDSASQGLGPSLLRGRLPTLLHLGRLALWGLGMCCYVFSAQQLQAAQVSPDDISLGFLVRAKGVVPGSTAPLEFLHITFQCFFAAFYLALSADVPPALLRHLFNCGRPGNSPMARLLPTMCIQASEGKDSSVAALLQKAEPHNLQITAAFLAGLLSREHWGLLAECQTSEKALLRRQACARWCLARSLRKHFHSIPPAAPGEAKSVHAMPGFIWLIRSLYEMQEERLARKAARGLNVGHLKLTFCSVGPTECAALAFVLQHLRRPVALQLDYNSVGDIGVEQLLPCLGVCKALYLRDNNISDRGICKLIECALHCEQLQKLALFNNKLTDGCAHSMAKLLACRQNFLALRLGNNYITAAGAQVLAEGLRGNTSLQFLGFWGNRVGDEGAQALAEALGDHQSLRWLSLVGNNIGSVGAQALALMLAKNVMLEELCLEENHLQDEGVCSLAEGLKKNSSLKILKLSNNCITYLGAEALLQALERNDTILEVWLRGNTFSLEEVDKLGCRDTRLLL DIP protein Q9HC29 original sequence version: 141 DIP protein Q9HC29 original sequence version: 77 DIP protein Q9HC29-1 original sequence version: 100 DIP protein Q9HC29-1 original sequence version: 106 mint homomint Induces NF-kappa-B via RICK (CARDIAK, RIP2) and IKK-gamma. Confers responsiveness to intracellular bacterial lipopolysaccharides (LPS) Defects in NOD2 are the cause of Blau syndrome (BS) [MIM:186580]. BS is a rare autosomal dominant disorder characterized by early-onset granulomatous arthritis, uveitis and skin rash Defects in NOD2 are a cause of susceptibility to Crohn disease (CD) [MIM:266600]. CD is a form of remitting inflammatory bowel disease (IBD). CD may involve any part of the gastrointestinal tract, but most frequently the terminal ileum and colon. Bowel inflammation is transmural and discontinuous. Crohn disease is commonly classified as autoimmune disease Defects in NOD2 are a cause of susceptibility to ulcerative colitis [MIM:191390]. It is a chronic inflammatory bowel disease. In ulcerative colitis, the inflammation is continuous and limited to rectal and colonic mucosal layers. Ulcerative colitis is commonly classified as autoimmune disease Defects in NOD2 are the cause of early-onset sarcoidosis (EOS) [MIM:609464]. Sarcoidosis is a multiorganic inflammatory disease with unknown etiology, characterized by the histologic features of non-caseating epithelioid granulomas. In childhood, 2 distinct types of sarcoidosis have been described. Usually the disease is detected in older children by chest radiography and the clinical manifestations are characterized by a classical triad of lung, lymph node, and eye involvement, similar to those in adults. In contrast, early-onset sarcoidosis (EOS), which usually appears in those younger than 4 years of age, is quite rare and has a distinct triad of skin, joint, and eye disorders, without apparent pulmonary involvement. Compared with an asymptomatic and sometimes naturally disappearing course of the disease in older children, EOS is progressive and in many cases causes severe complications, such as blindness, joint destruction, and visceral involvement 0037592D96D7DDFF nod2-vim-1 2 7 unspecified role unspecified role bait bait Q9HC29:p.Arg702Trp mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain R W Wed May 02 18:13:22 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg702trp' Tue Jun 30 16:33:08 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg702Trp' 6 unspecified role unspecified role prey prey physical association physical association 1B nod2-vim-2 1 7 unspecified role unspecified role bait bait region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 6 unspecified role unspecified role prey prey physical association physical association 1C nod2-vim-3 5 7 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 6 unspecified role unspecified role bait bait physical association physical association 1D vim-nod2-1 4 7 unspecified role unspecified role prey prey Ct_terminal_region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Q9HC29:p.Arg702Trp mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain R W Wed May 02 18:51:51 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg702trp' Tue Jun 30 18:06:13 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg702Trp' 6 unspecified role unspecified role bait bait physical association physical association 2 B/C, 4D, Supp fig 1. The NOD2-R702W mutant showed decreased binding and the L1007fs variant, which is an insertion/frameshift mutant truncated at amino acid 1007, did not bind to Vimentin. Withaferin A (WFA, CHEBI:69120 ), a steroidal lactone from the plant Withania somnifera and a Vimentin inhibitor. nod2-vim-4 3 7 unspecified role unspecified role neutral component neutral component 6 unspecified role unspecified role neutral component neutral component colocalization colocalization 3E