IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Solution structure of the dimerization domain of the eukaryotic stalk P1/P2 complex reveals the structural organization of eukaryotic stalk complex. Solution structure of the dimerization domain of the eukaryotic stalk P1/P2 complex reveals the structural organization of eukaryotic stalk complex. Nucleic acids research 2012 imex curation Lee KM., Yu CW., Chiu TY., Sze KH., Shaw PC., Wong KB. kbwong@cuhk.edu.hk 17-Sep-2015: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro nmr nuclear magnetic resonance NMR NMR nmr predetermined predetermined participant predetermined Lee KM., Yu CW., Chiu TY., Sze KH., Shaw PC., Wong KB. kbwong@cuhk.edu.hk Accepted 2015-JUL-30 by ORCHARD Nucleic acids research 2012 imex curation Solution structure of the dimerization domain of the eukaryotic stalk P1/P2 complex reveals the structural organization of eukaryotic stalk complex. Solution structure of the dimerization domain of the eukaryotic stalk P1/P2 complex reveals the structural organization of eukaryotic stalk complex. Nucleic acids research 2012 imex curation Lee KM., Yu CW., Chiu TY., Sze KH., Shaw PC., Wong KB. kbwong@cuhk.edu.hk 17-Sep-2015: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro pull down pull down weight by staining molecular weight estimation by staining weight by staining Lee KM., Yu CW., Chiu TY., Sze KH., Shaw PC., Wong KB. kbwong@cuhk.edu.hk Accepted 2015-JUL-30 by ORCHARD Nucleic acids research 2012 imex curation Solution structure of the dimerization domain of the eukaryotic stalk P1/P2 complex reveals the structural organization of eukaryotic stalk complex. Solution structure of the dimerization domain of the eukaryotic stalk P1/P2 complex reveals the structural organization of eukaryotic stalk complex. Nucleic acids research 2012 imex curation Lee KM., Yu CW., Chiu TY., Sze KH., Shaw PC., Wong KB. kbwong@cuhk.edu.hk 17-Sep-2015: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro sls static light scattering sls predetermined predetermined participant predetermined Lee KM., Yu CW., Chiu TY., Sze KH., Shaw PC., Wong KB. kbwong@cuhk.edu.hk Accepted 2015-JUL-30 by ORCHARD Nucleic acids research 2012 imex curation rla1_human 60S acidic ribosomal protein P1 RPLP1 RRP1 Large ribosomal subunit protein P1 protein protein human Homo sapiens Human MASVSELACIYSALILHDDEVTVTEDKINALIKAAGVNVEPFWPGLFAKALANVNIGSLICNVGAGGPAPAAGAAPAGGPAPSTAAAPAEEKKVEAKKEESEESDDDMGFGLFD 4C282AB8DCA079C8 rla0_human 60S acidic ribosomal protein P0 RPLP0 60S ribosomal protein L10E Large ribosomal subunit protein uL10 protein protein human Homo sapiens Human MPREDRATWKSNYFLKIIQLLDDYPKCFIVGADNVGSKQMQQIRMSLRGKAVVLMGKNTMMRKAIRGHLENNPALEKLLPHIRGNVGFVFTKEDLTEIRDMLLANKVPAAARAGAIAPCEVTVPAQNTGLGPEKTSFFQALGITTKISRGTIEILSDVQLIKTGDKVGASEATLLNMLNISPFSFGLVIQQVFDNGSIYNPEVLDITEETLHSRFLEGVRNVASVCLQIGYPTVASVPHSIINGYKRVLALSVETDYTFPLAEKVKAFLADPSAFVAAAPVAAATTAAPAAAAAPAKVEAKEESEESDEDMGFGLFD 255AD25571C51199 rla2_human 60S acidic ribosomal protein P2 RPLP2 RPP2 D11S2243E Renal carcinoma antigen NY-REN-44 Large ribosomal subunit protein P2 protein protein human Homo sapiens Human MRYVASYLLAALGGNSSPSAKDIKKILDSVGIEADDDRLNKVISELNGKNIEDVIAQGIGKLASVPAGGAVAVSAAPGSAAPAAGSAPAAAEEKKDEKKEESEESDDDMGFGLFD B5ECFE2510A756BF rplp1-rplp2-1 2 6 unspecified role unspecified role bait bait region rla2-his his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecoli Escherichia coli (strain K12) 4 unspecified role unspecified role prey prey region NTDP1 required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) direct interaction direct interaction Fig 4 A, Fig S2 rplp1-rplp2-2 1 6 unspecified role unspecified role neutral component neutral component region NTDP2 15n label 15n label 15N N15 15N N15 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region NTDP2 13c label 13c label C13 13C C13 13C undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region NTDP2 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 4 unspecified role unspecified role neutral component neutral component region NTDP1 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region NTDP1 15n label 15n label 15N N15 15N N15 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region NTDP1 13c label 13c label C13 13C C13 13C undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 16 18 direct interaction direct interaction Table 1 rplp0-rplp1-rplp2-3 3 5 unspecified role unspecified role neutral component neutral component n terminus rplp0 His tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range region PO sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 6 unspecified role unspecified role neutral component neutral component ecoli Escherichia coli (strain K12) 4 unspecified role unspecified role neutral component neutral component ecoli Escherichia coli (strain K12) physical association physical association Figure 5 rplp0-rplp1-1 3 5 unspecified role unspecified role neutral component neutral component n terminus rplp0 His tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range binding PO sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 6 unspecified role unspecified role neutral component neutral component ecoli Escherichia coli (strain K12) 4 unspecified role unspecified role neutral component neutral component ecoli Escherichia coli (strain K12) physical association physical association Figure 5 panel 3