IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ α7β2 nicotinic acetylcholine receptors assemble, function, and are activated primarily via their α7-α7 interfaces. α7β2 nicotinic acetylcholine receptors assemble, function, and are activated primarily via their α7-α7 interfaces. Molecular pharmacology 2012 imex curation Murray TA., Bertrand D., Papke RL., George AA., Pantoja R., Srinivasan R., Liu Q., Wu J., Whiteaker P., Lester HA., Lukas RJ., Murray TA., Bertrand D., Papke RL., George AA., Pantoja R., Srinivasan R., Liu Q., Wu J., Whiteaker P., Lester HA., Lukas RJ. Rettig et al 1987, PMID:3028608 Table 1, provides evidence for the cell line. It's a few references back from this paper as Murray et al 2009 (ref from this paper) has a typo in the parental cell line (SK-H-SK). bioengineer1@hotmail.com Only protein-protein interactions imex curation human-SH-EP1 human SH-EP1 epithelial-like cell line subcloned from the SK-N-SH neuroblastoma line Human sh-ep1 human epithelial-like cell line ATCC: CRL-2269 - Discontinued (parent line SK-N-SH available from other sources, e.g. ATCC HTB-11, Abcam ab3956) fret fluorescent resonance energy transfer FRET RET FRET analysis FRET RET FRET analysis fret tag visualisation tag visualisation Murray TA., Bertrand D., Papke RL., George AA., Pantoja R., Srinivasan R., Liu Q., Wu J., Whiteaker P., Lester HA., Lukas RJ., Murray TA., Bertrand D., Papke RL., George AA., Pantoja R., Srinivasan R., Liu Q., Wu J., Whiteaker P., Lester HA., Lukas RJ. Rettig et al 1987, PMID:3028608 Table 1, provides evidence for the cell line. It's a few references back from this paper as Murray et al 2009 (ref from this paper) has a typo in the parental cell line (SK-H-SK). Fluorescent tags were inserted into the cytoplasmic (C2) loop of the subunits between transmembrane domains M3 and M4 where they are thought to not affect channel gating or ligand recognition functions. bioengineer1@hotmail.com Accepted 2015-MAY-07 by ORCHARD Molecular pharmacology 2012 Only protein-protein interactions imex curation imex curation α7β2 nicotinic acetylcholine receptors assemble, function, and are activated primarily via their α7-α7 interfaces. α7β2 nicotinic acetylcholine receptors assemble, function, and are activated primarily via their α7-α7 interfaces. Molecular pharmacology 2012 imex curation Murray TA., Bertrand D., Papke RL., George AA., Pantoja R., Srinivasan R., Liu Q., Wu J., Whiteaker P., Lester HA., Lukas RJ., Murray TA., Bertrand D., Papke RL., George AA., Pantoja R., Srinivasan R., Liu Q., Wu J., Whiteaker P., Lester HA., Lukas RJ. Rettig et al 1987, PMID:3028608 Table 1, provides evidence for the cell line. It's a few references back from this paper as Murray et al 2009 (ref from this paper) has a typo in the parental cell line (SK-H-SK). bioengineer1@hotmail.com Only protein-protein interactions imex curation human-SH-EP1 human SH-EP1 epithelial-like cell line subcloned from the SK-N-SH neuroblastoma line Human sh-ep1 human epithelial-like cell line ATCC: CRL-2269 - Discontinued (parent line SK-N-SH available from other sources, e.g. ATCC HTB-11, Abcam ab3956) confocal microscopy confocal microscopy tag visualisation tag visualisation Murray TA., Bertrand D., Papke RL., George AA., Pantoja R., Srinivasan R., Liu Q., Wu J., Whiteaker P., Lester HA., Lukas RJ. Fluorescent tags were inserted into the cytoplasmic (C2) loop of the subunits between transmembrane domains M3 and M4 where they are thought to not affect channel gating or ligand recognition functions. bioengineer1@hotmail.com Accepted 2015-MAY-07 by ORCHARD Molecular pharmacology 2012 IMEx acha7_mouse Neuronal acetylcholine receptor subunit alpha-7 Chrna7 Acra7 protein protein mouse Mus musculus Mouse MCGRRGGIWLALAAALLHVSLQGEFQRRLYKELVKNYNPLERPVANDSQPLTVYFSLSLLQIMDVDEKNQVLTTNIWLQMSWTDHYLQWNMSEYPGVKNVRFPDGQIWKPDILLYNSADERFDATFHTNVLVNASGHCQYLPPGIFKSSCYIDVRWFPFDVQQCKLKFGSWSYGGWSLDLQMQEADISSYIPNGEWDLMGIPGKRNEKFYECCKEPYPDVTYTVTMRRRTLYYGLNLLIPCVLISALALLVFLLPADSGEKISLGITVLLSLTVFMLLVAEIMPATSDSVPLIAQYFASTMIIVGLSVVVTVIVLRYHHHDPDGGKMPKWTRIILLNWCAWFLRMKRPGEDKVRPACQHKPRRCSLASVELSAGAGPPTSNGNLLYIGFRGLEGMHCAPTPDSGVVCGRLACSPTHDEHLMHGTHPSDGDPDLAKILEEVRYIANRFRCQDESEVICSEWKFAACVVDRLCLMAFSVFTIICTIGILMSAPNFVEAVSKDFA DIP protein P49582 original sequence version: 91 C9312E5226D120E3 achb2_mouse Neuronal acetylcholine receptor subunit beta-2 Chrnb2 protein protein mouse Mus musculus Mouse MARCSNSMALLFSFGLLWLCSGVLGTDTEERLVEHLLDPSRYNKLIRPATNGSELVTVQLMVSLAQLISVHEREQIMTTNVWLTQEWEDYRLTWKPEDFDNMKKVRLPSKHIWLPDVVLYNNADGMYEVSFYSNAVVSYDGSIFWLPPAIYKSACKIEVKHFPFDQQNCTMKFRSWTYDRTEIDLVLKSDVASLDDFTPSGEWDIIALPGRRNENPDDSTYVDITYDFIIRRKPLFYTINLIIPCVLITSLAILVFYLPSDCGEKMTLCISVLLALTVFLLLISKIVPPTSLDVPLVGKYLMFTMVLVTFSIVTSVCVLNVHHRSPTTHTMAPWVKVVFLEKLPTLLFLQQPRHRCARQRLRLRRRQREREGAGTLFFREGPAADPCTCFVNPASMQGLAGAFQAEPAAAGLGRSMGPCSCGLREAVDGVRFIADHMRSEDDDQSVREDWKYVAMVIDRLFLWIFVFVCVFGTIGMFLQPLFQNYTATTFLHSDHSAPSSK DIP protein Q9ERK7 original sequence version: 69 7FFAD47BD86B4493 chrna7-chrnb2-1 2 4 unspecified role unspecified role unspecified role unspecified role cherry tag mcherry mcherry fluorescent protein tag mcherry undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 3 unspecified role unspecified role unspecified role unspecified role YFP tag yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined colocalization colocalization Fig. 1h chrna7-chrnb2-2 1 4 unspecified role unspecified role fluorescence donor fluorescence donor cherry tag mcherry mcherry fluorescent protein tag mcherry undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 3 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept YFP tag yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined proximity proximity close-contact colocalization neighbourhood interaction Fig. 2c, 2d, Table 1