UniProt UniProt http://www.uniprot.org PTEN, NHERF1 and PHLPP form a tumor suppressor network that is disabled in glioblastoma. PTEN, NHERF1 and PHLPP form a tumor suppressor network that is disabled in glioblastoma. Oncogene 2012 imex curation Molina JR., Agarwal NK., Morales FC., Hayashi Y., Aldape KD., Cote G., Georgescu MM., Molina JR., Agarwal NK., Morales FC., Hayashi Y., Aldape KD., Cote G., Georgescu MM. PHLPP1 C-terminal contstruct has been mapped to the UniProt canonical form as PHLPP1 isoforms only differ in the N-terminus; thus seq coordinates differ from the publication. Origin of PTEN construct according Takahaski Y et al, 2006 (PubMed 16456542). mgeorges@mdanderson.org 15-Sep-2016: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Molina JR., Agarwal NK., Morales FC., Hayashi Y., Aldape KD., Cote G., Georgescu MM., Molina JR., Agarwal NK., Morales FC., Hayashi Y., Aldape KD., Cote G., Georgescu MM. PHLPP1 C-terminal contstruct has been mapped to the UniProt canonical form as PHLPP1 isoforms only differ in the N-terminus; thus seq coordinates differ from the publication. Origin of PTEN construct according Takahaski Y et al, 2006 (PubMed 16456542). mgeorges@mdanderson.org mgeorges@manderson.org Accepted 2016-AUG-11 AT 10:59 BST AT 10:59 BST by PPORRAS Oncogene 2012 Only protein-protein interactions imex curation imex curation PTEN, NHERF1 and PHLPP form a tumor suppressor network that is disabled in glioblastoma. PTEN, NHERF1 and PHLPP form a tumor suppressor network that is disabled in glioblastoma. Oncogene 2012 imex curation Molina JR., Agarwal NK., Morales FC., Hayashi Y., Aldape KD., Cote G., Georgescu MM., Molina JR., Agarwal NK., Morales FC., Hayashi Y., Aldape KD., Cote G., Georgescu MM. PHLPP1 C-terminal contstruct has been mapped to the UniProt canonical form as PHLPP1 isoforms only differ in the N-terminus; thus seq coordinates differ from the publication. Origin of PTEN construct according Takahaski Y et al, 2006 (PubMed 16456542). mgeorges@mdanderson.org 15-Sep-2016: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Molina JR., Agarwal NK., Morales FC., Hayashi Y., Aldape KD., Cote G., Georgescu MM., Molina JR., Agarwal NK., Morales FC., Hayashi Y., Aldape KD., Cote G., Georgescu MM. PHLPP1 C-terminal contstruct has been mapped to the UniProt canonical form as PHLPP1 isoforms only differ in the N-terminus; thus seq coordinates differ from the publication. Origin of PTEN construct according Takahaski Y et al, 2006 (PubMed 16456542). mgeorges@mdanderson.org mgeorges@manderson.org Accepted 2016-AUG-11 AT 10:59 BST AT 10:59 BST by PPORRAS Oncogene 2012 Only protein-protein interactions imex curation IMEx MIMIx imex curation PTEN, NHERF1 and PHLPP form a tumor suppressor network that is disabled in glioblastoma. PTEN, NHERF1 and PHLPP form a tumor suppressor network that is disabled in glioblastoma. Oncogene 2012 imex curation Molina JR., Agarwal NK., Morales FC., Hayashi Y., Aldape KD., Cote G., Georgescu MM., Molina JR., Agarwal NK., Morales FC., Hayashi Y., Aldape KD., Cote G., Georgescu MM. PHLPP1 C-terminal contstruct has been mapped to the UniProt canonical form as PHLPP1 isoforms only differ in the N-terminus; thus seq coordinates differ from the publication. Origin of PTEN construct according Takahaski Y et al, 2006 (PubMed 16456542). mgeorges@mdanderson.org 15-Sep-2016: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Molina JR., Agarwal NK., Morales FC., Hayashi Y., Aldape KD., Cote G., Georgescu MM., Molina JR., Agarwal NK., Morales FC., Hayashi Y., Aldape KD., Cote G., Georgescu MM. PHLPP1 C-terminal contstruct has been mapped to the UniProt canonical form as PHLPP1 isoforms only differ in the N-terminus; thus seq coordinates differ from the publication. Origin of PTEN construct according Takahaski Y et al, 2006 (PubMed 16456542). mgeorges@mdanderson.org mgeorges@manderson.org Accepted 2016-AUG-11 AT 10:59 BST AT 10:59 BST by PPORRAS Oncogene 2012 Only protein-protein interactions imex curation IMEx imex curation PTEN, NHERF1 and PHLPP form a tumor suppressor network that is disabled in glioblastoma. PTEN, NHERF1 and PHLPP form a tumor suppressor network that is disabled in glioblastoma. Oncogene 2012 imex curation Molina JR., Agarwal NK., Morales FC., Hayashi Y., Aldape KD., Cote G., Georgescu MM., Molina JR., Agarwal NK., Morales FC., Hayashi Y., Aldape KD., Cote G., Georgescu MM. PHLPP1 C-terminal contstruct has been mapped to the UniProt canonical form as PHLPP1 isoforms only differ in the N-terminus; thus seq coordinates differ from the publication. Origin of PTEN construct according Takahaski Y et al, 2006 (PubMed 16456542). mgeorges@mdanderson.org 15-Sep-2016: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-ln18 Homo sapiens LN18 glioblastoma cell line ln18 LN18 human glioblastoma cell line The LN-18 cell line was established in 1976 from cells taken from a patient with a right temporal lobe glioma. The cells are poorly differentiated. The cells are negative for glial fibrillary acidic proteins and S100 (S-100) protein. The cells exhibit mutated p53 (TP53) and possible homozygous deletions in the p16 and p14ARF tumor suppressor genes. They have a wild-type PTEN gene. Stimulation of the cells with Fas ligand lead to apoptotic cell death within 16 hours. The cells were also killed by puromycin in a dose dependent manner. Bcl-2 protects these cells from Fas ligand-induced cell death but was shown to have only a small protective effect on puromycin-induced apoptosis. This cell line is used in studies on apoptosis. (Source of this information is the ATCC entry). fluorescence imaging fluorescence microscopy fluorescence imaging immunostaining immunostaining Molina JR., Agarwal NK., Morales FC., Hayashi Y., Aldape KD., Cote G., Georgescu MM., Molina JR., Agarwal NK., Morales FC., Hayashi Y., Aldape KD., Cote G., Georgescu MM. PHLPP1 C-terminal contstruct has been mapped to the UniProt canonical form as PHLPP1 isoforms only differ in the N-terminus; thus seq coordinates differ from the publication. Origin of PTEN construct according Takahaski Y et al, 2006 (PubMed 16456542). mgeorges@mdanderson.org mgeorges@manderson.org Accepted 2016-AUG-11 AT 10:59 BST AT 10:59 BST by PPORRAS Oncogene 2012 Only protein-protein interactions imex curation IMEx imex curation PTEN, NHERF1 and PHLPP form a tumor suppressor network that is disabled in glioblastoma. PTEN, NHERF1 and PHLPP form a tumor suppressor network that is disabled in glioblastoma. Oncogene 2012 imex curation Molina JR., Agarwal NK., Morales FC., Hayashi Y., Aldape KD., Cote G., Georgescu MM., Molina JR., Agarwal NK., Morales FC., Hayashi Y., Aldape KD., Cote G., Georgescu MM. PHLPP1 C-terminal contstruct has been mapped to the UniProt canonical form as PHLPP1 isoforms only differ in the N-terminus; thus seq coordinates differ from the publication. Origin of PTEN construct according Takahaski Y et al, 2006 (PubMed 16456542). mgeorges@mdanderson.org 15-Sep-2016: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro filter binding filter binding Filter overlay assay Filter overlay assay anti tag western anti tag western blot anti tag western Molina JR., Agarwal NK., Morales FC., Hayashi Y., Aldape KD., Cote G., Georgescu MM., Molina JR., Agarwal NK., Morales FC., Hayashi Y., Aldape KD., Cote G., Georgescu MM. PHLPP1 C-terminal contstruct has been mapped to the UniProt canonical form as PHLPP1 isoforms only differ in the N-terminus; thus seq coordinates differ from the publication. Origin of PTEN construct according Takahaski Y et al, 2006 (PubMed 16456542). mgeorges@mdanderson.org mgeorges@manderson.org Accepted 2016-AUG-11 AT 10:59 BST AT 10:59 BST by PPORRAS Oncogene 2012 Only protein-protein interactions imex curation imex curation PTEN, NHERF1 and PHLPP form a tumor suppressor network that is disabled in glioblastoma. PTEN, NHERF1 and PHLPP form a tumor suppressor network that is disabled in glioblastoma. Oncogene 2012 imex curation Molina JR., Agarwal NK., Morales FC., Hayashi Y., Aldape KD., Cote G., Georgescu MM., Molina JR., Agarwal NK., Morales FC., Hayashi Y., Aldape KD., Cote G., Georgescu MM. PHLPP1 C-terminal contstruct has been mapped to the UniProt canonical form as PHLPP1 isoforms only differ in the N-terminus; thus seq coordinates differ from the publication. Origin of PTEN construct according Takahaski Y et al, 2006 (PubMed 16456542). mgeorges@mdanderson.org 15-Sep-2016: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-ln_229 Homo sapiens malignant glioma cell line LN-229 ln_229 Human malignant glioma cell line LN-229 anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Molina JR., Agarwal NK., Morales FC., Hayashi Y., Aldape KD., Cote G., Georgescu MM., Molina JR., Agarwal NK., Morales FC., Hayashi Y., Aldape KD., Cote G., Georgescu MM. PHLPP1 C-terminal contstruct has been mapped to the UniProt canonical form as PHLPP1 isoforms only differ in the N-terminus; thus seq coordinates differ from the publication. Origin of PTEN construct according Takahaski Y et al, 2006 (PubMed 16456542). mgeorges@mdanderson.org mgeorges@manderson.org Accepted 2016-AUG-11 AT 10:59 BST AT 10:59 BST by PPORRAS Oncogene 2012 Only protein-protein interactions imex curation IMEx imex curation o60346-2 PH domain leucine-rich repeat-containing protein phosphatase 1 PHLPP1 KIAA0606 PHLPP PLEKHE1 SCOP Pleckstrin homology domain-containing family E member 1 Suprachiasmatic nucleus circadian oscillatory protein alpha protein protein human Homo sapiens Human MDSEIGCLIRFYAGKPHSTGSSERIQLSGMYNVRKGKMQLPVNRWTRRQVILCGTCLIVSSVKDSLTGKMHVLPLIGGKVEEVKKHQHCLAFSSSGPQSQTYYICFDTFTEYLRWLRQVSKVASQRISSVDLSCCSLEHLPANLFYSQDLTHLNLKQNFLRQNPSLPAARGLNELQRFTKLKSLNLSNNHLGDFPLAVCSIPTLAELNVSCNALRSVPAAVGVMHNLQTFLLDGNFLQSLPAELENMKQLSYLGLSFNEFTDIPEVLEKLTAVDKLCMSGNCVETLRLQALRKMPHIKHVDLRLNVIRKLIADEVDFLQHVTQLDLRDNKLGDLDAMIFNNIEVLHCERNQLVTLDICGYFLKALYASSNELVQLDVYPVPNYLSYMDVSRNRLENVPEWVCESRKLEVLDIGHNQICELPARLFCNSSLRKLLAGHNQLARLPERLERTSVEVLDVQHNQLLELPPNLLMKADSLRFLNASANKLESLPPATLSEETNSILQELYLTNNSLTDKCVPLLTGHPHLKILHMAYNRLQSFPASKMAKLEELEEIDLSGNKLKAIPTTIMNCRRMHTVIAHSNCIEVFPEVMQLPEIKCVDLSCNELSEVTLPENLPPKLQELDLTGNPRLVLDHKTLELLNNIRCFKIDQPSTGDASGAPAVWSHGYTEASGVKNKLCVAALSVNNFCDNREALYGVFDGDRNVEVPYLLQCTMSDILAEELQKTKNEEEYMVNTFIVMQRKLGTAGQKLGGAAVLCHIKHDPVDPGGSFTLTSANVGKCQTVLCRNGKPLPLSRSYIMSCEEELKRIKQHKAIITEDGKVNGVTESTRILGYTFLHPSVVPRPHVQSVLLTPQDEFFILGSKGLWDSLSVEEAVEAVRNVPDALAAAKKLCTLAQSYGCHDSISAVVVQLSVTEDSFCCCELSAGGAVPPPSPGIFPPSVNMVIKDRPSDGLGVPSSSSGMASEISSELSTSEMSSEVGSTASDEPPPGALSENSPAYPSEQRCMLHPICLSNSFQRQLSSATFSSAFSDNGLDSDDEEPIEGVFTNGSRVEVEVDIHCSRAKEKEKQQHLLQVPAEASDEGIVISANEDEPGLPRKADFSAVGTIGRRRANGSVAPQERSHNVIEVATDAPLRKPGGYFAAPAQPDPDDQFIIPPELEEEVKEIMKHHQEQQQQQQPPPPPQLQPQLPRHYQLDQLPDYYDTPL CDBECB2FDA9C7064 q9wtr8-1 PH domain leucine-rich repeat protein phosphatase 1 Pleckstrin homology domain-containing family E member 1 Suprachiasmatic nucleus circadian oscillatory protein beta Phlpp1 Phlpp Plekhe1 Scop protein protein rat Rattus norvegicus (Rat) Rat MEPAAAAPAQRLADPTGEDRAPAAAAAAEGGRSPDSVLSAAAPSGGNGGAAREEAPCEAPPGPLPGRAGGTGRRRRRGVPQPAAGGAAPVTAAGGGANSLLLRRGRLKRNLSAAASSSSSPSSASSAAGGLPASCSASASLCTRSLDRKTLLQKHRQLLQLQPSDRDWVRHQLQRGCVHVFDRHMASSYLRPVLCTLDTTAAEVAARLLQLGHKGGGVVKVLGHGPPPAAAPAASDQTPATELGRDVEPPPSSSTVGAVRGPARAPPADLPLPGGAWTRCAPRVNPAPSDSSPGELFAGGPCSPSRAPRPASDTESFSLSPSAESVSDRLDPYSSGGGSSSSSEELEADPATVLTGPSGPPHHPVRSSQPRPPSPKTSALLQPKAPTGVDGTGLVVGEGPGDDKAVAAAAPGVPLWTPGRIRETVQKTSSPPSLYVQLHGETTRRLEADEKPLQIQNDYLFQLGFGELWRVQEEGMDSEIGCLIRFYAGKPHSTGSSERIQLSGMYNVRKGKMQLPVNRWTRRQVILCGTCLIVSSVKDSSSGKMHVLPLIGGKVEEVKKHQHCLAFSSSGPQSQTYYICFDTFTEYLRWLRQVSKVASQRISSVDLSCCSLEHLPANLFYSQDLTHLNLKQNFLRQNPSLPAARGLGELQRFTKLKSLNLSNNHLGAFPSAVCSIPTLAELNVSCNALQEVPAAVGAMQNLQTFLLDGNFLQSLPAELENMHQLSYLGLSFNEFTDIPEVLEKLTAVDKLCMAGNCMETLRLQALRRMPHIKHVDLRLNILRKLITDEVDFLQHVTQLDLRDNKLGDLDAMIFNNIEVLHCERNQLVTLNICGYFLKALYASSNELVQLDVYPVPNYLSYMDVSRNCLESVPEWVCESRKLEVLDIGHNQICELPARLFCNSSLRKLLAGHNRLARLPERLERTSVEVLDVQHNQIIELPPNLLMKADSLRFLNASANKLETLPPATLSEETSSILQELYLTNNSLTDKCVPLLTGHPRLKILHMAYNRLQSFPASKMAKLEELEEIDISGNKLKAIPTTIMNCRRMHTVIAHSNCIEVFPEVMQLPEVKCVDLSCNELSEITLPENLPPKLQELDLTGNPRLALDHKSLELLNNIRCFKIDQPSAGDASGAPAVWSHGYTEASGVKNKLCVAALSVNNFRDNREALYGVFDGDRNVEVPYLLQCTMSDILAEELQKTKNEEEYMVNTFIVMQRKLGTAGQKLGGAAVLCHIRHDPVDLGGSFTLTSANVGKCQTVLCRNGKPLSLSRSYTMSCEEERKRIKQHKAIITEDGKVNGVTESTRILGYTFLHPSVVPRPHVQSVLLTPQDEFFILGSKGLWDSLSIEEAVEAVRNVPDALAAAKKLCTLAQSYGCHDSISAVVVQLSVTEDSFCCCELSVGGSMPPPSPGIFPPSVSMVIKDRPSDGLGVPSSSSGMASEISSELSTSEMSSEVGSTASDEPPSGALSESSPAYPSEQRCMLHPVCLSNSFQRQLSSATFSSAFSDNGLDSDDEEPIEGVFSNGSRVEVEVDIHCSRAKEKERQQHLLQVPAEASDEGIVISANEDESGLSKKTDISAVGTIGRRRANGSVPPQERSHNVIEVATDAPLRKPGGYFAAPAQPDPDDQFIIPPELEEEVKEIMKHHQEQQQQQQQQQQQQQQQPPPPPQPPQAQAQAQAQAQRPFQMDHLPDCYDTPL B4C3CC777B8BB6A9 phlp1_human PH domain leucine-rich repeat-containing protein phosphatase 1 PHLPP1 KIAA0606 PHLPP PLEKHE1 SCOP Pleckstrin homology domain-containing family E member 1 Suprachiasmatic nucleus circadian oscillatory protein protein protein human Homo sapiens Human MEPAAAATVQRLPELGREDRASAPAAAAAAAAAAAAAAAALAAAAGGGRSPEPALTPAAPSGGNGSGSGAREEAPGEAPPGPLPGRAGGAGRRRRRGAPQPIAGGAAPVPGAGGGANSLLLRRGRLKRNLSAAAAAASSSSSSSAAAASHSPGAAGLPASCSASASLCTRSLDRKTLLLKHRQTLQLQPSDRDWVRHQLQRGCVHVFDRHMASTYLRPVLCTLDTTAGEVAARLLQLGHKGGGVVKVLGQGPGAAAAREPAEPPPEAGPRLAPPEPRDSEVPPARSAPGAFGGPPRAPPADLPLPVGGPGGWSRRASPAPSDSSPGEPFVGGPVSSPRAPRPVVSDTESFSLSPSAESVSDRLDPYSSGGGSSSSSEELEADAASAPTGVPGQPRRPGHPAQPLPLPQTASSPQPQQKAPRAIDSPGGAVREGSCEEKAAAAVAPGGLQSTPGRSGVTAEKAPPPPPPPTLYVQLHGETTRRLEAEEKPLQIQNDYLFQLGFGELWRVQEEGMDSEIGCLIRFYAGKPHSTGSSERIQLSGMYNVRKGKMQLPVNRWTRRQVILCGTCLIVSSVKDSLTGKMHVLPLIGGKVEEVKKHQHCLAFSSSGPQSQTYYICFDTFTEYLRWLRQVSKVASQRISSVDLSCCSLEHLPANLFYSQDLTHLNLKQNFLRQNPSLPAARGLNELQRFTKLKSLNLSNNHLGDFPLAVCSIPTLAELNVSCNALRSVPAAVGVMHNLQTFLLDGNFLQSLPAELENMKQLSYLGLSFNEFTDIPEVLEKLTAVDKLCMSGNCVETLRLQALRKMPHIKHVDLRLNVIRKLIADEVDFLQHVTQLDLRDNKLGDLDAMIFNNIEVLHCERNQLVTLDICGYFLKALYASSNELVQLDVYPVPNYLSYMDVSRNRLENVPEWVCESRKLEVLDIGHNQICELPARLFCNSSLRKLLAGHNQLARLPERLERTSVEVLDVQHNQLLELPPNLLMKADSLRFLNASANKLESLPPATLSEETNSILQELYLTNNSLTDKCVPLLTGHPHLKILHMAYNRLQSFPASKMAKLEELEEIDLSGNKLKAIPTTIMNCRRMHTVIAHSNCIEVFPEVMQLPEIKCVDLSCNELSEVTLPENLPPKLQELDLTGNPRLVLDHKTLELLNNIRCFKIDQPSTGDASGAPAVWSHGYTEASGVKNKLCVAALSVNNFCDNREALYGVFDGDRNVEVPYLLQCTMSDILAEELQKTKNEEEYMVNTFIVMQRKLGTAGQKLGGAAVLCHIKHDPVDPGGSFTLTSANVGKCQTVLCRNGKPLPLSRSYIMSCEEELKRIKQHKAIITEDGKVNGVTESTRILGYTFLHPSVVPRPHVQSVLLTPQDEFFILGSKGLWDSLSVEEAVEAVRNVPDALAAAKKLCTLAQSYGCHDSISAVVVQLSVTEDSFCCCELSAGGAVPPPSPGIFPPSVNMVIKDRPSDGLGVPSSSSGMASEISSELSTSEMSSEVGSTASDEPPPGALSENSPAYPSEQRCMLHPICLSNSFQRQLSSATFSSAFSDNGLDSDDEEPIEGVFTNGSRVEVEVDIHCSRAKEKEKQQHLLQVPAEASDEGIVISANEDEPGLPRKADFSAVGTIGRRRANGSVAPQERSHNVIEVATDAPLRKPGGYFAAPAQPDPDDQFIIPPELEEEVKEIMKHHQEQQQQQQPPPPPQLQPQLPRHYQLDQLPDYYDTPL A4AB53F334EA8AB6 pten_human Phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN Phosphatase and tensin homolog Mutated in multiple advanced cancers 1 PTEN MMAC1 TEP1 protein protein human Homo sapiens Human MTAIIKEIVSRNKRRYQEDGFDLDLTYIYPNIIAMGFPAERLEGVYRNNIDDVVRFLDSKHKNHYKIYNLCAERHYDTAKFNCRVAQYPFEDHNPPQLELIKPFCEDLDQWLSEDDNHVAAIHCKAGKGRTGVMICAYLLHRGKFLKAQEALDFYGEVRTRDKKGVTIPSQRRYVYYYSYLLKNHLDYRPVALLFHKMMFETIPMFSGGTCNPQFVVCQLKVKIYSSNSGPTRREDKFMYFEFPQPLPVCGDIKVEFFHKQNKMLKKDKMFHFWVNTFFIPGPEETSEKVENGSLCDQEIDSICSIERADNDKEYLVLTLTKNDLDKANKDKANRYFSPNFKVKLYFTKTVEEPSNPEASSSTSVTPDVSDNEPDHYRYSDTTDSDPENEPFDEDQHTQITKV 75F97C3DD6802BA9 nhrf1_human Na(+)/H(+) exchange regulatory cofactor NHE-RF1 SLC9A3R1 NHERF NHERF1 Ezrin-radixin-moesin-binding phosphoprotein 50 Regulatory cofactor of Na(+)/H(+) exchanger Sodium-hydrogen exchanger regulatory factor 1 Solute carrier family 9 isoform A3 regulatory factor 1 protein protein human Homo sapiens Human MSADAAAGAPLPRLCCLEKGPNGYGFHLHGEKGKLGQYIRLVEPGSPAEKAGLLAGDRLVEVNGENVEKETHQQVVSRIRAALNAVRLLVVDPETDEQLQKLGVQVREELLRAQEAPGQAEPPAAAEVQGAGNENEPREADKSHPEQRELRPRLCTMKKGPSGYGFNLHSDKSKPGQFIRSVDPDSPAEASGLRAQDRIVEVNGVCMEGKQHGDVVSAIRAGGDETKLLVVDRETDEFFKKCRVIPSQEHLNGPLPVPFTNGEIQKENSREALAEAALESPRPALVRSASSDTSEELNSQDSPPKQDSTAPSSTSSSDPILDFNISLAMAKERAHQKRSSKRAPQMDWSKKNELFSNL E33AF87016D37A65 phlp2_human PH domain leucine-rich repeat-containing protein phosphatase 2 PHLPP2 KIAA0931 PHLPPL PH domain leucine-rich repeat-containing protein phosphatase-like protein protein human Homo sapiens Human MKRNGSRNCLNRRSRFGSRERDWLREDVKRGCVYLYGADTTTATTTTTTSSSSSSSSSSSDLHLVLCTVETPASEICAGEGRESLYLQLHGDLVRRLEPTERPLQIVYDYLSRLGFDDPVRIQEEATNPDLGCMIRFYGEKPCHMDRLDRILLSGIYNVRKGKTQLHKWAERLVVLCGTCLIVSSVKDCQTGKMHILPLVGGKIEEVKRRQYSLAFSSAGAQAQTYHVSFETLAEYQRWQRQASKVVSQRISTVDLSCYSLEEVPEHLFYSQDITYLNLRHNFMQLERPGGLDTLYKFSQLKGLNLSHNKLGLFPILLCEISTLTELNLSCNGFHDLPSQIGNLLNLQTLCLDGNFLTTLPEELGNLQQLSSLGISFNNFSQIPEVYEKLTMLDRVVMAGNCLEVLNLGVLNRMNHIKHVDLRMNHLKTMVIENLEGNKHITHVDLRDNRLTDLDLSSLCSLEQLHCGRNQLRELTLSGFSLRTLYASSNRLTAVNVYPVPSLLTFLDLSRNLLECVPDWACEAKKIEVLDVSYNLLTEVPVRILSSLSLRKLMLGHNHVQNLPTLVEHIPLEVLDLQHNALTRLPDTLFSKALNLRYLNASANSLESLPSACTGEESLSMLQLLYLTNNLLTDQCIPVLVGHLHLRILHLANNQLQTFPASKLNKLEQLEELNLSGNKLKTIPTTIANCKRLHTLVAHSNNISIFPEILQLPQIQFVDLSCNDLTEILIPEALPATLQDLDLTGNTNLVLEHKTLDIFSHITTLKIDQKPLPTTDSTVTSTFWSHGLAEMAGQRNKLCVSALAMDSFAEGVGAVYGMFDGDRNEELPRLLQCTMADVLLEEVQQSTNDTVFMANTFLVSHRKLGMAGQKLGSSALLCYIRPDTADPASSFSLTVANVGTCQAVLCRGGKPVPLSKVFSLEQDPEEAQRVKDQKAIITEDNKVNGVTCCTRMLGCTYLYPWILPKPHISSTPLTIQDELLILGNKALWEHLSYTEAVNAVRHVQDPLAAAKKLCTLAQSYGCQDNVGAMVVYLNIGEEGCTCEMNGLTLPGPVGFASTTTIKDAPKPATPSSSSGIASEFSSEMSTSEVSSEVGSTASDEHNAGGLDTALLPRPERRCSLHPTPTSGLFQRQPSSATFSSNQSDNGLDSDDDQPVEGVITNGSKVEVEVDIHCCRGRDLENSPPLIESSPTLCSEEHARGSCFGIRRQNSVNSGMLLPMSKDRMELQKSPSTSCLYGKKLSNGSIVPLEDSLNLIEVATEVPKRKTGYFAAPTQMEPEDQFVVPHDLEEEVKEQMKQHQDSRLEPEPHEEDRTEPPEEFDTAL DIP protein Q6ZVD8 original sequence version: 104 04FC8550C2E22A6D slc9a3r1-phlpp1-1 1 9 unspecified role unspecified role prey prey human-ln_229 Homo sapiens malignant glioma cell line LN-229 ln_229 Human malignant glioma cell line LN-229 11 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined pdz_1 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) physical association physical association S2 slc9a3r1-phlpp2-1 1 11 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined pdz_2 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 12 unspecified role unspecified role prey prey cell lysate cell lysate human-ln18 Homo sapiens LN18 glioblastoma cell line ln18 LN18 human glioblastoma cell line The LN-18 cell line was established in 1976 from cells taken from a patient with a right temporal lobe glioma. The cells are poorly differentiated. The cells are negative for glial fibrillary acidic proteins and S100 (S-100) protein. The cells exhibit mutated p53 (TP53) and possible homozygous deletions in the p16 and p14ARF tumor suppressor genes. They have a wild-type PTEN gene. Stimulation of the cells with Fas ligand lead to apoptotic cell death within 16 hours. The cells were also killed by puromycin in a dose dependent manner. Bcl-2 protects these cells from Fas ligand-induced cell death but was shown to have only a small protective effect on puromycin-induced apoptosis. This cell line is used in studies on apoptosis. (Source of this information is the ATCC entry). physical association physical association S2 slc9a3r1-phlpp2-2 5 11 unspecified role unspecified role bait bait pdz_1 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined pdz_2 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain O14745:p.Gln37Arg mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain Q R Wed May 02 14:44:30 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gln37arg' Tue Jun 30 18:32:31 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln37Arg' ecoli Escherichia coli (strain K12) 12 unspecified role unspecified role prey prey in vitro translated in vitro translated protein in vitro translated region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 28 25 direct interaction direct interaction 1b slc9a3r1-phlpp1-2 5 7 unspecified role unspecified role prey prey region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 11 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined pdz_1 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain pdz_2 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain O14745:p.Gln37Arg mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain Q R Wed May 02 15:07:16 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gln37arg' Tue Jun 30 18:32:12 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln37Arg' ecoli Escherichia coli (strain K12) 38 40 direct interaction direct interaction 1b slc9a3r1-phlpp2-3 5 11 unspecified role unspecified role prey prey in vitro translated in vitro translated protein in vitro translated region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range pdz_2 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 12 unspecified role unspecified role bait bait cell lysate cell lysate region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) direct interaction direct interaction 1c slc9a3r1-phlpp1-3 5 7 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 11 unspecified role unspecified role prey prey in vitro translated in vitro translated protein in vitro translated region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range pdz_1 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain direct interaction direct interaction 1c pten-slc9a3r1-1 5 11 unspecified role unspecified role ancillary ancillary region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 12 unspecified role unspecified role prey prey in vitro translated in vitro translated protein in vitro translated n-terminus myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 10 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) physical association physical association 1d pten-phlpp1-1 5 7 unspecified role unspecified role prey prey n-terminus myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 11 unspecified role unspecified role ancillary ancillary region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 10 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) physical association physical association 1d phlpp1-slc9a3r1-1 2 11 unspecified role unspecified role prey prey nucl transfection nucleic acid transfection nucl transfection region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 7 unspecified role unspecified role bait bait nucl transfection nucleic acid transfection nucl transfection region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain physical association physical association 2a phlpp1-slc9a3r1-2 3 11 unspecified role unspecified role bait bait nucl transfection nucleic acid transfection nucl transfection region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 7 unspecified role unspecified role prey prey nucl transfection nucleic acid transfection nucl transfection region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain physical association physical association 2a phlpp1-slc9a3r1-3 3 11 unspecified role unspecified role bait bait nucl transfection nucleic acid transfection nucl transfection region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 8 unspecified role unspecified role prey prey nucl transfection nucleic acid transfection nucl transfection physical association physical association 2b slc9a3r1-1 3 12 unspecified role unspecified role prey prey nucl transfection nucleic acid transfection nucl transfection 11 unspecified role unspecified role bait bait nucl transfection nucleic acid transfection nucl transfection region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 2b phlpp1-slc9a3r1-4 6 9 unspecified role unspecified role prey prey 11 unspecified role unspecified role bait bait physical association physical association 2c phlpp1-slc9a3r1-5 4 11 unspecified role unspecified role neutral component neutral component nucl transfection nucleic acid transfection nucl transfection myr tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 7 unspecified role unspecified role neutral component neutral component nucl transfection nucleic acid transfection nucl transfection region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain colocalization colocalization 2d