IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ The Bowen-Conradi syndrome protein Nep1 (Emg1) has a dual role in eukaryotic ribosome biogenesis, as an essential assembly factor and in the methylation of Ψ1191 in yeast 18S rRNA. The Bowen-Conradi syndrome protein Nep1 (Emg1) has a dual role in eukaryotic ribosome biogenesis, as an essential assembly factor and in the methylation of Ψ1191 in yeast 18S rRNA. Nucleic acids research 2011 imex curation Meyer B., Wurm JP., Kötter P., Leisegang MS., Schilling V., Buchhaupt M., Held M., Bahr U., Karas M., Heckel A., Bohnsack MT., Wöhnert J., Entian KD. entian@bio.uni-frankfurt.de 17-Sep-2015: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Meyer B., Wurm JP., Kötter P., Leisegang MS., Schilling V., Buchhaupt M., Held M., Bahr U., Karas M., Heckel A., Bohnsack MT., Wöhnert J., Entian KD. entian@bio.uni-frankfurt.de Accepted 2015-AUG-10 by ORCHARD Nucleic acids research 2011 imex curation The Bowen-Conradi syndrome protein Nep1 (Emg1) has a dual role in eukaryotic ribosome biogenesis, as an essential assembly factor and in the methylation of Ψ1191 in yeast 18S rRNA. The Bowen-Conradi syndrome protein Nep1 (Emg1) has a dual role in eukaryotic ribosome biogenesis, as an essential assembly factor and in the methylation of Ψ1191 in yeast 18S rRNA. Nucleic acids research 2011 imex curation Meyer B., Wurm JP., Kötter P., Leisegang MS., Schilling V., Buchhaupt M., Held M., Bahr U., Karas M., Heckel A., Bohnsack MT., Wöhnert J., Entian KD. entian@bio.uni-frankfurt.de 17-Sep-2015: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro enzymatic study enzymatic study sequence tag sequence tag identification sequence tag Meyer B., Wurm JP., Kötter P., Leisegang MS., Schilling V., Buchhaupt M., Held M., Bahr U., Karas M., Heckel A., Bohnsack MT., Wöhnert J., Entian KD. entian@bio.uni-frankfurt.de Accepted 2015-AUG-10 by ORCHARD Nucleic acids research 2011 imex curation The Bowen-Conradi syndrome protein Nep1 (Emg1) has a dual role in eukaryotic ribosome biogenesis, as an essential assembly factor and in the methylation of Ψ1191 in yeast 18S rRNA. The Bowen-Conradi syndrome protein Nep1 (Emg1) has a dual role in eukaryotic ribosome biogenesis, as an essential assembly factor and in the methylation of Ψ1191 in yeast 18S rRNA. Nucleic acids research 2011 imex curation Meyer B., Wurm JP., Kötter P., Leisegang MS., Schilling V., Buchhaupt M., Held M., Bahr U., Karas M., Heckel A., Bohnsack MT., Wöhnert J., Entian KD. entian@bio.uni-frankfurt.de 17-Sep-2015: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro fps fluorescence polarization spectroscopy Fluorescence anisotropy FPS FPS Fluorescence anisotropy fps tag fluorescence tag visualisation by fluorescence tag fluorescence Meyer B., Wurm JP., Kötter P., Leisegang MS., Schilling V., Buchhaupt M., Held M., Bahr U., Karas M., Heckel A., Bohnsack MT., Wöhnert J., Entian KD. entian@bio.uni-frankfurt.de Accepted 2015-AUG-10 by ORCHARD Nucleic acids research 2011 imex curation nep1_yeast Ribosomal RNA small subunit methyltransferase NEP1 EMG1 NEP1 YLR186W L9470.5 Nucleolar essential protein 1 18S rRNA (pseudouridine(1189)-N1)-methyltransferase Essential for mitotic growth protein 1 protein protein yeast Saccharomyces cerevisiae Baker's yeast MVEDSRVRDALKGGDQKALPASLVPQAPPVLTSKDKITKRMIVVLAMASLETHKISSNGPGGDKYVLLNCDDHQGLLKKMGRDISEARPDITHQCLLTLLDSPINKAGKLQVYIQTSRGILIEVNPTVRIPRTFKRFSGLMVQLLHKLSIRSVNSEEKLLKVIKNPITDHLPTKCRKVTLSFDAPVIRVQDYIEKLDDDESICVFVGAMARGKDNFADEYVDEKVGLSNYPLSASVACSKFCHGAEDAWNIL 4A8FCDA812051AD1 4A8FCDA812051AD1 rrna 18s yeast Ribosomal RNA 18S Yeast rrna ribosomal rna rRNA rrna yeast Saccharomyces cerevisiae Baker's yeast UAUCUGGUUGAUCCUGCCAGUAGUCAUAUGCUUGUCUCAAAGAUUAAGCCAUGCAUGUCUAAGUAUAAGCAAUUUAUACAGUGAAACUGCGAAUGGCUCAUUAAAUCAGUUAUCGUUUAUUUGAUAGUUCCUUUACUACAUGGUAUAACUGUGGUAAUUCUAGAGCUAAUACAUGCUUAAAAUCUCGACCCUUUGGAAGAGAUGUAUUUAUUAGAUAAAAAAUCAAUGUCUUCGGACUCUUUGAUGAUUCAUAAUAACUUUUCGAAUCGCAUGGCCUUGUGCUGGCGAUGGUUCAUUCAAAUUUCUGCCCUAUCAACUUUCGAUGGUAGGAUAGUGGCCUACCAUGGUUUCAACGGGUAACGGGGAAUAAGGGUUCGAUUCCGGAGAGGGAGCCUGAGAAACGGCUACCACAUCCAAGGAAGGCAGCAGGCGCGCAAAUUACCCAAUCCUAAUUCAGGGAGGUAGUGACAAUAAAUAACGAUACAGGGCCCAUUCGGGUCUUGUAAUUGGAAUGAGUACAAUGUAAAUACCUUAACGAGGAACAAUUGGAGGGCAAGUCUGGUGCCAGCAGCCGCGGUAAUUCCAGCUCCAAUAGCGUAUAUUAAAGUUGUUGCAGUUAAAAAGCUCGUAGUUGAACUUUGGGCCCGGUUGGCCGGUCCGAUUUUUUCGUGUACUGGAUUUCCAACGGGGCCUUUCCUUCUGGCUAACCUUGAGUCCUUGUGGCUCUUGGCGAACCAGGACUUUUACUUUGAAAAAAUUAGAGUGUUCAAAGCAGGCGUAUUGCUCGAAUAUAUUAGCAUGGAAUAAUAGAAUAGGACGUUUGGUUCUAUUUUGUUGGUUUCUAGGACCAUCGUAAUGAUUAAUAGGGACGGUCGGGGGCAUCAGUAUUCAAUUGUCAGAGGUGAAAUUCUUGGAUUUAUUGAAGACUAACUACUGCGAAAGCAUUUGCCAAGGACGUUUUCAUUAAUCAAGAACGAAAGUUAGGGGAUCGAAGAUGAUCAGAUACCGUCGUAGUCUUAACCAUAAACUAUGCCGACUAGGGAUCGGGUGGUGUUUUUUUAAUGACCCACUCGGCACCUUACGAGAAAUCAAAGUCUUUGGGUUCUGGGGGGAGUAUGGUCGCAAGGCUGAAACUUAAAGGAAUUGACGGAAGGGCACCACCAGGAGUGGAGCCUGCGGCUUAAUUUGACUCAACACGGGGAAACUCACCAGGUCCAGACACAAUAAGGAUUGACAGAUUGAGAGCUCUUUCUUGAUUUUGUGGGUGGUGGUGCAUGGCCGUUCUUAGUUGGUGGAGUGAUUUGUCUGCUUAAUUGCGAUAACGAACGAGACCUUAACCUACUAAAUAGUGGUGCUAGCAUUUGCUGGUUAUCCACUUCUUAGAGGGACUAUCGGUUUCAAGCCGAUGGAAGUUUGAGGCAAUAACAGGUCUGUGAUGCCCUUAGACGUUCUGGGCCGCACGCGCGCUACACUGACGGAGCCAGCGAGUCUAACCUUGGCCGAGAGGUCUUGGUAAUCUUGUGAAACUCCGUCGUGCUGGGGAUAGAGCAUUGUAAUUAUUGCUCUUCAACGAGGAAUUCCUAGUAAGCGCAAGUCAUCAGCUUGCGUUGAUUACGUCCCUGCCCUUUGUACACACCGCCCGUCGCUAGUACCGAUUGAAUGGCUUAGUGAGGCCUCAGGAUCUGCUUAGAGAAGGGGGCAACUCCAUCUCAGAGCGGAGAAUUUGGACAAACUUGGUCAUUUAGAGGAACUAAAAGUCGUAACAAGGUUUCCGUAGGUGAACCUGCGGAAGGAUCAUUA nep1_nep1-1 1 4 unspecified role unspecified role neutral component neutral component Q06287:p.Asp90Gly mutation increasing mutation increasing interaction mutation increasing certain certain sequence position certain certain certain sequence position certain D G Wed May 02 15:41:05 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp90gly' Tue Jun 30 15:37:00 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp90Gly' His tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) direct interaction direct interaction Fig5A nep1_18s-1 3 5 unspecified role unspecified role neutral component neutral component binding region sufficient to bind sufficient binding region sufficient to bind predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain tag fluorescein fluorescein label fluorescein certain certain sequence position certain certain certain sequence position certain chemical synthesis Chemical synthesis (Chemical synthesis) 4 unspecified role unspecified role neutral component neutral component Q06287:p.Asp90Gly mutation increasing mutation increasing interaction mutation increasing certain certain sequence position certain certain certain sequence position certain D G Wed May 02 14:40:11 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp90gly' Tue Jun 30 18:58:45 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp90Gly' ecoli Escherichia coli (strain K12) direct interaction direct interaction Fig5B nep1_18s-2 2 5 enzyme target enzyme target substrate substrate neutral component neutral component binding region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain methylated u-1191 rna chemical modification RNA chemical modification RNA chemical modification post-transcriptional modification rna chemical modification certain certain sequence position certain certain certain sequence position certain chemical synthesis Chemical synthesis (Chemical synthesis) 4 enzyme enzyme neutral component neutral component ecoli Escherichia coli (strain K12) methylation methylation reaction methylation Fig6