IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Cracking pre-40S ribosomal subunit structure by systematic analyses of RNA-protein cross-linking. Cracking pre-40S ribosomal subunit structure by systematic analyses of RNA-protein cross-linking. The EMBO journal 2010 imex curation Granneman S., Petfalski E., Swiatkowska A., Tollervey D. d.tollervey@ed.ac.uk 17-Sep-2015: Contacted by IntAct-Help. Only protein-protein interactions imex curation yeast Saccharomyces cerevisiae Baker's yeast crac crac CRAC cross-linking and analysis of cDNAs crac partial rna sequence partial RNA sequence identification partial rna sequence Granneman S., Petfalski E., Swiatkowska A., Tollervey D. The Host organism was yeast strain BY4741 d.tollervey@ed.ac.uk Accepted 2015-JUN-30 by ORCHARD The EMBO journal 2010 imex curation Cracking pre-40S ribosomal subunit structure by systematic analyses of RNA-protein cross-linking. Cracking pre-40S ribosomal subunit structure by systematic analyses of RNA-protein cross-linking. The EMBO journal 2010 imex curation Granneman S., Petfalski E., Swiatkowska A., Tollervey D. d.tollervey@ed.ac.uk 17-Sep-2015: Contacted by IntAct-Help. Only protein-protein interactions imex curation yeast Saccharomyces cerevisiae Baker's yeast affinity chrom affinity chromatography technology Affinity purification Affinity purification affinity chrom rt-pcr reverse transcription pcr RT-PCR Granneman S., Petfalski E., Swiatkowska A., Tollervey D. d.tollervey@ed.ac.uk Accepted 2015-JUL-06 by ORCHARD The EMBO journal 2010 imex curation tsr1_yeast Ribosome biogenesis protein TSR1 TSR1 20S rRNA accumulation protein 1 YDL060W protein protein yeast Saccharomyces cerevisiae Baker's yeast MAGHSHRSSLKNGHKSYKSKHASKGALKRLYKGKVEKEPVGTGKPDKQVSKLQRKNKAKQLRAQRILDSIENRKLFEGKNGAAKIITIVPLVNDLDPLDILYKLLKCADDEGIMVQEVDSKRIFNVHIKKFKSNLKIIIPDMTNFLNILDCAKVADFVVFGLSGVQEVDEEFGEQIIRALELQGIASYIGVISNLSAVHEKEKFQLDVKQSLESYFKHFFPSEERVYNLEKNSDALNVLRTLCQRLPRSINWRDNRGYVVADFVDFVETSPDSGDLVIEGTVRGIGFNANRLVHIPDFGDFQLNKIEKISESSQKRKIIKEKATDSLSLELDLQTVFESNMNRDTLDEYAPEGTEDWSDYDEDFEYDGLTTARYDDHGFLPGREQTSKKAAVPKGTSDYQAKWYLDDVIDANEEEEAEQTNGKDETMMEIDDEMMVEQDNEEVAGDEEYDIEDNEGFEELSPEEEERQLREFRDMEKEDREFPDEIELEPSESAIERLKRYRGLKNLYNCDWQVDEKDPSSPAEWKRLLRIGNYKNTKNRIIKETKNEAQAIAGDRIRMFIRFPKFLLEKIQDPKQLLFAVYGLLLHEHKNAVVNFSLQRWEQYDKPVPSQEPIVVQYGVRRYTIQPLFSQGSNSPNNVHKYERFLHPDTVSVATCIAPVDFTQSPAIFFKPSPTDAKNIELIGHGTFLNADHSRILAKRAILTGHPFRFHKTVVTVRYMFFRPEDVEWFKSIPLFTKSGRSGFIKESLGTHGYFKATFDGKLSAQDVVAMSLYKRMWPMPSLPWNGM 71BA5461E8A858C7 71BA5461E8A858C7 rio2_yeast Serine/threonine-protein kinase RIO2 N1342 YNL207W RIO2 protein protein yeast Saccharomyces cerevisiae Baker's yeast MKLDTSHMRYLTTDDFRVLQAVEQGSRSHEVVPTPLIHQISGMRSQSGTNRAISDLAKLSLISKMRNVKYDGYRLTYNGIDYLALKTMLNRDTVYSVGNTIGVGKESDIYKVSDKNGNPRVMKIHRLGRTSFHSVRNNRDYLKKSNQGANWMHLSRLAANKEYQFMSMLYSKGFKVPEPFDNSRHIVVMELIEGYPMRRLRKHKNIPKLYSDLMCFIVDLANSGLIHCDFNEFNIMIKDKLEDENDCGFVVIDFPQCISIQHQDADYYFQRDVDCIRRFFKKKLKYEPKPDSSMLDTEGFGDGYKYAYPDFKRDVKRTDNLDELVQASGFSKKHPGDRGLETAVESMRNAVYNSDDDMSNDEAEEENGEGDYSEEDEYYDSELDNESSEDDSEDAQEEENERIIEALSSGVENLKMDKLGNYILE 02117D0328118E8D 02117D0328118E8D nob1_yeast 20S-pre-rRNA D-site endonuclease NOB1 Pre-rRNA-processing endonuclease NOB1 NIN1-binding protein NOB1 YOR29-07 YOR056C protein protein yeast Saccharomyces cerevisiae Baker's yeast MTENQTAHVRALILDATPLITQSYTHYQNYAQSFYTTPTVFQEIKDAQARKNLEIWQSLGTLKLVHPSENSIAKVSTFAKLTGDYSVLSANDLHILALTYELEIKLNNGDWRLRKKPGDALDASKADVGTDGKQKLTEDNKKEEDSESVPKKKNKRRGGKKQKAKREAREAREAENANLELESKAEEHVEEAGSKEQICNDENIKESSDLNEVFEDADDDGDWITPENLTEAIIKDSGEDTTGSLGVEASEEDRHVALNRPENQVALATGDFAVQNVALQMNLNLMNFMSGLKIKRIRNYMLRCHACFKIFPLPKDGKPKHFCASCGGQGTLLRCAVSVDSRTGNVTPHLKSNFQWNNRGNRYSVASPLSKNSQKRYGKKGHVHSKPQENVILREDQKEYEKVIKQEEWTRRHNEKILNNWIGGGSADNYISPFAITGLKQHNVRIGKGRYVNSSKRRS 96DEB818BB490A98 96DEB818BB490A98 enp1_yeast Essential nuclear protein 1 ENP1 MEG1 YBR247C YBR1635 protein protein yeast Saccharomyces cerevisiae Baker's yeast MARASSTKARKQRHDPLLKDLDAAQGTLKKINKKKLAQNDAANHDAANEEDGYIDSKASRKILQLAKEQQDEIEGEELAESERNKQFEARFTTMSYDDEDEDEDEDEEAFGEDISDFEPEGDYKEEEEIVEIDEEDAAMFEQYFKKSDDFNSLSGSYNLADKIMASIREKESQVEDMQDDEPLANEQNTSRGNISSGLKSGEGVALPEKVIKAYTTVGSILKTWTHGKLPKLFKVIPSLRNWQDVIYVTNPEEWSPHVVYEATKLFVSNLTAKESQKFINLILLERFRDNIETSEDHSLNYHIYRAVKKSLYKPSAFFKGFLFPLVETGCNVREATIAGSVLAKVSVPALHSSAALSYLLRLPFSPPTTVFIKILLDKKYALPYQTVDDCVYYFMRFRILDDGSNGEDATRVLPVIWHKAFLTFAQRYKNDITQDQRDFLLETVRQRGHKDIGPEIRRELLAGASREFVDPQEANDDLMIDVN 399A415131294637 399A415131294637 rrna 20s precursor ribosomal RNA 20S yeast rrna ribosomal rna rRNA rrna yeast Saccharomyces cerevisiae Baker's yeast UAUCUGGUUGAUCCUGCCAGUAGUCAUAUGCUUGUCUCAAAGAUUAAGCCAUGCAUGUCUAAGUAUAAGCAAUUUAUACAGUGAAACUGCGAAUGGCUCAUUAAAUCAGUUAUCGUUUAUUUGAUAGUUCCUUUACUACAUGGUAUAACUGUGGUAAUUCUAGAGCUAAUACAUGCUUAAAAUCUCGACCCUUUGGAAGAGAUGUAUUUAUUAGAUAAAAAAUCAAUGUCUUCGGACUCUUUGAUGAUUCAUAAUAACUUUUCGAAUCGCAUGGCCUUGUGCUGGCGAUGGUUCAUUCAAAUUUCUGCCCUAUCAACUUUCGAUGGUAGGAUAGUGGCCUACCAUGGUUUCAACGGGUAACGGGGAAUAAGGGUUCGAUUCCGGAGAGGGAGCCUGAGAAACGGCUACCACAUCCAAGGAAGGCAGCAGGCGCGCAAAUUACCCAAUCCUAAUUCAGGGAGGUAGUGACAAUAAAUAACGAUACAGGGCCCAUUCGGGUCUUGUAAUUGGAAUGAGUACAAUGUAAAUACCUUAACGAGGAACAAUUGGAGGGCAAGUCUGGUGCCAGCAGCCGCGGUAAUUCCAGCUCCAAUAGCGUAUAUUAAAGUUGUUGCAGUUAAAAAGCUCGUAGUUGAACUUUGGGCCCGGUUGGCCGGUCCGAUUUUUUCGUGUACUGGAUUUCCAACGGGGCCUUUCCUUCUGGCUAACCUUGAGUCCUUGUGGCUCUUGGCGAACCAGGACUUUUACUUUGAAAAAAUUAGAGUGUUCAAAGCAGGCGUAUUGCUCGAAUAUAUUAGCAUGGAAUAAUAGAAUAGGACGUUUGGUUCUAUUUUGUUGGUUUCUAGGACCAUCGUAAUGAUUAAUAGGGACGGUCGGGGGCAUCAGUAUUCAAUUGUCAGAGGUGAAAUUCUUGGAUUUAUUGAAGACUAACUACUGCGAAAGCAUUUGCCAAGGACGUUUUCAUUAAUCAAGAACGAAAGUUAGGGGAUCGAAGAUGAUCAGAUACCGUCGUAGUCUUAACCAUAAACUAUGCCGACUAGGGAUCGGGUGGUGUUUUUUUAAUGACCCACUCGGCACCUUACGAGAAAUCAAAGUCUUUGGGUUCUGGGGGGAGUAUGGUCGCAAGGCUGAAACUUAAAGGAAUUGACGGAAGGGCACCACCAGGAGUGGAGCCUGCGGCUUAAUUUGACUCAACACGGGGAAACUCACCAGGUCCAGACACAAUAAGGAUUGACAGAUUGAGAGCUCUUUCUUGAUUUUGUGGGUGGUGGUGCAUGGCCGUUCUUAGUUGGUGGAGUGAUUUGUCUGCUUAAUUGCGAUAACGAACGAGACCUUAACCUACUAAAUAGUGGUGCUAGCAUUUGCUGGUUAUCCACUUCUUAGAGGGACUAUCGGUUUCAAGCCGAUGGAAGUUUGAGGCAAUAACAGGUCUGUGAUGCCCUUAGACGUUCUGGGCCGCACGCGCGCUACACUGACGGAGCCAGCGAGUCUAACCUUGGCCGAGAGGUCUUGGUAAUCUUGUGAAACUCCGUCGUGCUGGGGAUAGAGCAUUGUAAUUAUUGCUCUUCAACGAGGAAUUCCUAGUAAGCGCAAGUCAUCAGCUUGCGUUGAUUACGUCCCUGCCCUUUGUACACACCGCCCGUCGCUAGUACCGAUUGAAUGGCUUAGUGAGGCCUCAGGAUCUGCUUAGAGAAGGGGGCAACUCCAUCUCAGAGCGGAGAAUUUGGACAAACUUGGUCAUUUAGAGGAACUAAAAGUCGUAACAAGGUUUCCGUAGGUGAACCUGCGGAAGGAUCAUUAAAGAAAUUUAAUAAUUUUGAAAAUGGAUUUUUUUGUUUUGGCAAGAGCAUGAGAGCUUUUACUGGGCAAGAAGACAAGAGAUGGAGAGUCCAGCCGGGCCUGCGCUUAAGUGCGCGGUCUUGCUAGGCUUGUAAGUUUCUUUCUUGCUAUUCCAAACGGUGAGAGAUUUCUGUGCUUUUGUUAUAGGACAAUUAAAACCGUUUCAAUACAAC ltv1_yeast Protein LTV1 LTV1 YKL143W YKL2 Low-temperature viability protein 1 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSKKFSSKNSQRYVVVHRPHDDPSFYDTDASAHVLVPVSNPNKTSPEADLRKKDVSSTKPKGRRAHVGEAALYGINFDDSEYDYTQHLKPIGLDPENSIFIASKGNEQKVEKKNIEDLFIEPKYRRDEIEKDDALPVFQRGMAKPEYLLHQQDTTDEIRGFKPDMNPALREVLEALEDEAYVVNDDVVVEDISKKTQLQGDNYGEEEKEDDIFAQLLGSGEAKDEDEFEDEFDEWDIDNVENFEDENYVKEMAQFDNIENLEDLENIDYQADVRRFQKDNSILEKHNSDDEFSNAGLDSVNPSEEEDVLGELPSIQDKSKTGKKKRKSRQKKGAMSDVSGFSMSSSAIARTETMTVLDDQYDQIINGYENYEEELEEDEEQNYQPFDMSAERSDFESMLDDFLDNYELESGGRKLAKKDKEIERLKEAADEVSKGKLSQRRNRERQEKKKLEKVTNTLSSLKF F432C239B3B0A68A F432C239B3B0A68A dim1_yeast Dimethyladenosine transferase YPL266W DIM1 S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase 18S rRNA dimethylase 18S rRNA (adenine(1779)-N(6)/adenine(1780)-N(6))-dimethyltransferase protein protein yeast Saccharomyces cerevisiae Baker's yeast MGKAAKKKYSGATSSKQVSAEKHLSSVFKFNTDLGQHILKNPLVAQGIVDKAQIRPSDVVLEVGPGTGNLTVRILEQAKNVVAVEMDPRMAAELTKRVRGTPVEKKLEIMLGDFMKTELPYFDICISNTPYQISSPLVFKLINQPRPPRVSILMFQREFALRLLARPGDSLYCRLSANVQMWANVTHIMKVGKNNFRPPPQVESSVVRLEIKNPRPQVDYNEWDGLLRIVFVRKNRTISAGFKSTTVMDILEKNYKTFLAMNNEMVDDTKGSMHDVVKEKIDTVLKETDLGDKRAGKCDQNDFLRLLYAFHQVGIHFS EE77C9DF574A042A EE77C9DF574A042A dim1_rrna20s-1 1 7 unspecified role unspecified role prey prey binding region binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain 9 unspecified role unspecified role bait bait region His Tev proteinA tag tandem tag tandem tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range physical association physical association Fig1A, suppl tab S4 tsr1_rrna20s-1 1 7 unspecified role unspecified role prey prey binding region binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain 3 unspecified role unspecified role bait bait region His Tev proteinA tag tandem tag tandem tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range physical association physical association Fig1, suppl tab 9 nob1_rrna 20s-1 1 7 unspecified role unspecified role prey prey binding region binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain 5 unspecified role unspecified role bait bait region His Tev proteinA tag tandem tag tandem tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range undefined mutation D15N mutation mutation mutation certain certain sequence position certain certain certain sequence position certain Wed Jul 06 17:10:28 BST 2016 Sequence change details about this feature cannot be ascertained or do not fit with the current version of the referenced protein, so they have been deleted as a result of our quality control procedures. The original label was 'mutation' yeast Saccharomyces cerevisiae Baker's yeast physical association physical association Fig1A, suppl tab 7, suppl fig 4 ltv1_rrna20s-1 1 7 unspecified role unspecified role prey prey binding region binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain 8 unspecified role unspecified role bait bait region His Tev proteinA tag tandem tag tandem tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range physical association physical association Fig1, suppl tab 6 enp1_rrna20s-1 1 7 unspecified role unspecified role prey prey binding region binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain 6 unspecified role unspecified role bait bait region His Tev proteinA tag tandem tag tandem tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range physical association physical association Fig1 S5 rio2-1 1 7 unspecified role unspecified role prey prey binding region binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain 4 unspecified role unspecified role bait bait region His Tev proteinA tag tandem tag tandem tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range physical association physical association fig1, suppl. tab 8 nob1-rrna-1 2 5 unspecified role unspecified role bait bait 7 unspecified role unspecified role prey prey D-site region D-site region sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Fig4 B,C