IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Molecular mechanisms of HipA-mediated multidrug tolerance and its neutralization by HipB. Molecular mechanisms of HipA-mediated multidrug tolerance and its neutralization by HipB. Science (New York, N.Y.) 2009 imex curation Schumacher MA., Piro KM., Xu W., Hansen S., Lewis K., Brennan RG. Interactions involving EF-TU could not be captured as this could not be mapped to a single gene product. rgbrenna@mdanderson.org,maschuma@mdanderson.org 21-10-2015: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Schumacher MA., Piro KM., Xu W., Hansen S., Lewis K., Brennan RG. Interactions involving EF-TU could not be captured as this could not be mapped to a single gene product. rgbrenna@mdanderson.org,maschuma@mdanderson.org gro.nosrednadm@annerbgr, gro.nosrednadm@amuhcsam Accepted 2015-OCT-09 AT 11:52 BST AT 11:52 BST by MGT Science (New York, N.Y.) 2009 Only protein-protein interactions imex curation imex curation I don't see how the tags can have been removed, there is no cleavage site built into the construct. I assume this is another think not included on the new PDB website. Also, as evidence for EF-TU as being A is a bit circumstantial, I'm using it as an excuse not to include the interaction - the whole thing is challenged in PMID: 24095282 as an erroneous observation. Molecular mechanisms of HipA-mediated multidrug tolerance and its neutralization by HipB. Molecular mechanisms of HipA-mediated multidrug tolerance and its neutralization by HipB. Science (New York, N.Y.) 2009 imex curation Schumacher MA., Piro KM., Xu W., Hansen S., Lewis K., Brennan RG. Interactions involving EF-TU could not be captured as this could not be mapped to a single gene product. rgbrenna@mdanderson.org,maschuma@mdanderson.org 21-10-2015: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Schumacher MA., Piro KM., Xu W., Hansen S., Lewis K., Brennan RG. Interactions involving EF-TU could not be captured as this could not be mapped to a single gene product. rgbrenna@mdanderson.org,maschuma@mdanderson.org gro.nosrednadm@annerbgr, gro.nosrednadm@amuhcsam Accepted 2015-OCT-09 AT 11:52 BST AT 11:52 BST by MGT Science (New York, N.Y.) 2009 Only protein-protein interactions imex curation imex curation I don't see how the tags can have been removed, there is no cleavage site built into the construct. I assume this is another think not included on the new PDB website. Also, as evidence for EF-TU as being A is a bit circumstantial, I'm using it as an excuse not to include the interaction - the whole thing is challenged in PMID: 24095282 as an erroneous observation. atp ATP Adenosine 5'-triphosphate Adenosine triphosphate ADENOSINE-5'-TRIPHOSPHATE Adephos Adetol Adynol Atipi ATP Atriphos Cardenosine Fosfobion Glucobasin H4atp Myotriphos Triadenyl Triphosphaden adenosine 5'-(tetrahydrogen triphosphate) small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) hipb_ecoli Antitoxin HipB hipB b1508 JW1501 protein protein ecoli Escherichia coli (strain K12) MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISNFENNPDNTTLTTFFKILQSLELSMTLCDAKNASPESTEQQNLEW DIP protein P23873 original sequence version: 114 63C0E13C1C06CBDA hipa_ecoli Serine/threonine-protein kinase toxin HipA hipA b1507 JW1500 Toxin HipA protein protein ecoli Escherichia coli (strain K12) MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLSLPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYHAKSRQPFDLLSEIGRDSVGAVTLIPEDETVTHPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTALLRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVPDAEIIKAGNVRALAVERFDRRWNAERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIARIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQAGGSYRLTPFYDIISAFPVLGGTGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLRFPEVQMHEILSDFARMIPAALDNVKTSLPTDFPENVVTAVESNVLRLHGRLSREYGSK DIP protein P23874 original sequence version: 119 DIP protein P23874 original sequence version: null 378771C4CAB55319 actatccccttaaggggatag ds dna double stranded deoxyribonucleic acid ds DNA ds dna chemical synthesis Chemical synthesis (Chemical synthesis) ACTATCCCCTTAAGGGGATAG hipa-hipb-1 2 6 unspecified role unspecified role neutral component neutral component chemical synthesis Chemical synthesis (Chemical synthesis) 4 unspecified role unspecified role neutral component neutral component purified purified n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) 5 unspecified role unspecified role neutral component neutral component purified purified region se(s)met L-selenomethionine Delta:S(-1)Se(1) Se(S)Met Selenium replaces sulphur semetm undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range undefined mutation mutation mutation certain certain sequence position certain certain certain sequence position certain Wed Jul 06 17:06:22 BST 2016 Sequence change details about this feature cannot be ascertained or do not fit with the current version of the referenced protein, so they have been deleted as a result of our quality control procedures. The original label was 'asp309gln' ecoli Escherichia coli (strain K12) physical association physical association Supp Table 1 Rworking 0.228, Rfree 0.285 2.68A hipa-hipb-2 1 3 unspecified role unspecified role neutral component neutral component chemical synthesis Chemical synthesis (Chemical synthesis) 4 unspecified role unspecified role neutral component neutral component purified purified n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) 5 unspecified role unspecified role neutral component neutral component purified purified n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range undefined mutation mutation mutation certain certain sequence position certain certain certain sequence position certain Wed Jul 06 17:07:09 BST 2016 Sequence change details about this feature cannot be ascertained or do not fit with the current version of the referenced protein, so they have been deleted as a result of our quality control procedures. The original label was 'asp309gln' ecoli Escherichia coli (strain K12) direct interaction direct interaction Supp Figure 2 Atp was added to an existing HIPA-HIPB complex atp-hipa-1 1 3 unspecified role unspecified role neutral component neutral component chemical synthesis Chemical synthesis (Chemical synthesis) 5 unspecified role unspecified role neutral component neutral component purified purified n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range undefined mutation mutation mutation certain certain sequence position certain certain certain sequence position certain Wed Jul 06 17:08:41 BST 2016 Sequence change details about this feature cannot be ascertained or do not fit with the current version of the referenced protein, so they have been deleted as a result of our quality control procedures. The original label was 'asp309gln' ecoli Escherichia coli (strain K12) direct interaction direct interaction Supp Figure 2 actatccccttaaggggatag-atp-1 2 3 unspecified role unspecified role neutral component neutral component 6 unspecified role unspecified role neutral component neutral component chemical synthesis Chemical synthesis (Chemical synthesis) 4 unspecified role unspecified role neutral component neutral component purified purified n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) 5 unspecified role unspecified role neutral component neutral component purified purified region se(s)met L-selenomethionine Delta:S(-1)Se(1) Se(S)Met Selenium replaces sulphur semetm undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range undefined mutation mutation mutation certain certain sequence position certain certain certain sequence position certain Wed Jul 06 16:24:18 BST 2016 Sequence change details about this feature cannot be ascertained or do not fit with the current version of the referenced protein, so they have been deleted as a result of our quality control procedures. The original label was 'asp309gln' ecoli Escherichia coli (strain K12) physical association physical association Supp Table 1 Rworking 0.265, Rfree 0.283 2.98A atp-hipa-2 2 3 unspecified role unspecified role neutral component neutral component 5 unspecified role unspecified role neutral component neutral component purified purified region se(s)met L-selenomethionine Delta:S(-1)Se(1) Se(S)Met Selenium replaces sulphur semetm undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range undefined mutation mutation mutation certain certain sequence position certain certain certain sequence position certain Wed Jul 06 16:27:27 BST 2016 Sequence change details about this feature cannot be ascertained or do not fit with the current version of the referenced protein, so they have been deleted as a result of our quality control procedures. The original label was 'asp309gln' ecoli Escherichia coli (strain K12) physical association physical association Supp Table 1 Rworking 0.185, Rfree 0.217 1.66A