UniProt UniProt http://www.uniprot.org HMGB1 is a cofactor in mammalian base excision repair. HMGB1 is a cofactor in mammalian base excision repair. Molecular cell 2007 imex curation Prasad R., Liu Y., Deterding LJ., Poltoratsky VP., Kedar PS., Horton JK., Kanno S., Asagoshi K., Hou EW., Khodyreva SN., Lavrik OI., Tomer KB., Yasui A., Wilson SH. Transfected Flag-POLB/Polbeta has been described in PubMed 10197627 by the authors. FEN1 used in CoIP exp with purified proteins cannot be mapped as source information is missing. Host system for purified POLB described in Pubmed 8594388 (not available online) is not specified. wilson5@niehs.nih.gov 22-Feb-2016: Contacted by IntAct-Help. Only protein-protein interactions mouse-embryonic_fibroblast Mus musculus embryonic fibroblasts embryonic_fibroblast Embryonic fibroblast Fibroblasts taken from an embryonic as opposed to a foetal or adult source. Foetus is defined as the stage at which tissue diferentiation is initiated. Tissue taken from an embryonic as opposed to a foetal or adult source. Embryo is defined as the development stage prior to organ formation. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Prasad R., Liu Y., Deterding LJ., Poltoratsky VP., Kedar PS., Horton JK., Kanno S., Asagoshi K., Hou EW., Khodyreva SN., Lavrik OI., Tomer KB., Yasui A., Wilson SH. Polbeta null MEF cells expressing (human) Flag-tagged POLB/Polbeta have been used Transfected Flag-POLB/Polbeta has been described in PubMed 10197627 by the authors. FEN1 used in CoIP exp with purified proteins cannot be mapped as source information is missing. Host system for purified POLB described in Pubmed 8594388 (not available online) is not specified. wilson5@niehs.nih.gov Accepted 2016-JAN-29 AT 08:58 GMT AT 08:58 GMT by ORCHARD Molecular cell 2007 imex curation HMGB1 is a cofactor in mammalian base excision repair. HMGB1 is a cofactor in mammalian base excision repair. Molecular cell 2007 imex curation Prasad R., Liu Y., Deterding LJ., Poltoratsky VP., Kedar PS., Horton JK., Kanno S., Asagoshi K., Hou EW., Khodyreva SN., Lavrik OI., Tomer KB., Yasui A., Wilson SH. Transfected Flag-POLB/Polbeta has been described in PubMed 10197627 by the authors. FEN1 used in CoIP exp with purified proteins cannot be mapped as source information is missing. Host system for purified POLB described in Pubmed 8594388 (not available online) is not specified. wilson5@niehs.nih.gov 22-Feb-2016: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Prasad R., Liu Y., Deterding LJ., Poltoratsky VP., Kedar PS., Horton JK., Kanno S., Asagoshi K., Hou EW., Khodyreva SN., Lavrik OI., Tomer KB., Yasui A., Wilson SH. Transfected Flag-POLB/Polbeta has been described in PubMed 10197627 by the authors. FEN1 used in CoIP exp with purified proteins cannot be mapped as source information is missing. Host system for purified POLB described in Pubmed 8594388 (not available online) is not specified. wilson5@niehs.nih.gov Accepted 2016-JAN-29 AT 08:58 GMT AT 08:58 GMT by ORCHARD Molecular cell 2007 imex curation apex1_mouse DNA-(apurinic or apyrimidinic site) endonuclease Apex1 Ape Apex REF-1 Redox factor-1 Ref1 Apurinic-apyrimidinic endonuclease 1 APEX nuclease protein protein mouse Mus musculus Mouse MPKRGKKAAADDGEEPKSEPETKKSKGAAKKTEKEAAGEGPVLYEDPPDQKTSPSGKSATLKICSWNVDGLRAWIKKKGLDWVKEEAPDILCLQETKCSENKLPAELQELPGLTHQYWSAPSDKEGYSGVGLLSRQCPLKVSYGIGEEEHDQEGRVIVAEFESFVLVTAYVPNAGRGLVRLEYRQRWDEAFRKFLKDLASRKPLVLCGDLNVAHEEIDLRNPKGNKKNAGFTPQERQGFGELLQAVPLADSFRHLYPNTAYAYTFWTYMMNARSKNVGWRLDYFLLSHSLLPALCDSKIRSKALGSDHCPITLYLAL mint Repairs oxidative DNA damages in vitro. May have a role in protection against cell lethality and suppression of mutations. Removes the blocking groups from the 3'-termini of the DNA strand breaks generated by ionizing radiations and bleomycin CF086691FAC89C4A dpolb_human DNA polymerase beta POLB protein protein human Homo sapiens Human MSKRKAPQETLNGGITDMLTELANFEKNVSQAIHKYNAYRKAASVIAKYPHKIKSGAEAKKLPGVGTKIAEKIDEFLATGKLRKLEKIRQDDTSSSINFLTRVSGIGPSAARKFVDEGIKTLEDLRKNEDKLNHHQRIGLKYFGDFEKRIPREEMLQMQDIVLNEVKKVDSEYIATVCGSFRRGAESSGDMDVLLTHPSFTSESTKQPKLLHQVVEQLQKVHFITDTLSKGETKFMGVCQLPSKNDEKEYPHRRIDIRLIPKDQYYCGVLYFTGSDIFNKNMRAHALEKGFTINEYTIRPLGVTGVAGEPLPVDSEKDIFDYIQWKYREPKDRSE FF9A6D0E6F9A9487 hmgb1_human High mobility group protein B1 HMGB1 HMG1 High mobility group protein 1 protein protein human Homo sapiens Human MGKGDPKKPRGKMSSYAFFVQTCREEHKKKHPDASVNFSEFSKKCSERWKTMSAKEKGKFEDMAKADKARYEREMKTYIPPKGETKKKFKDPNAPKRPPSAFFLFCSEYRPKIKGEHPGLSIGDVAKKLGEMWNNTAADDKQPYEKKAAKLKEKYEKDIAAYRAKGKPDAAKKGVVKAEKSKKKKEEEEDEEDEEDEEEEEDEEDEDEEEDDDDE DIP protein P09429 original sequence version: 141 8A868CF277D417B5 hmgb1_mouse High mobility group protein B1 Hmgb1 Hmg-1 Hmg1 High mobility group protein 1 protein protein mouse Mus musculus Mouse MGKGDPKKPRGKMSSYAFFVQTCREEHKKKHPDASVNFSEFSKKCSERWKTMSAKEKGKFEDMAKADKARYEREMKTYIPPKGETKKKFKDPNAPKRPPSAFFLFCSEYRPKIKGEHPGLSIGDVAKKLGEMWNNTAADDKQPYEKKAAKLKEKYEKDIAAYRAKGKPDAAKKGVVKAEKSKKKKEEEDDEEDEEDEEEEEEEEDEDEEEDDDDE 8A868DE266D552B5 fen1_mouse Flap endonuclease 1 Flap structure-specific endonuclease 1 Fen1 Fen-1 protein protein mouse Mus musculus Mouse MGIHGLAKLIADVAPSAIRENDIKSYFGRKVAIDASMSIYQFLIAVRQGGDVLQNEEGETTSLMGMFYRTIRMENGIKPVYVFDGKPPQLKSGELAKRSERRAEAEKQLQQAQEAGMEEEVEKFTKRLVKVTKQHNDECKHLLSLMGIPYLDAPSEAEASCAALAKAGKVYAAATEDMDCLTFGSPVLMRHLTASEAKKLPIQEFHLSRVLQELGLNQEQFVDLCILLGSDYCESIRGIGAKRAVDLIQKHKSIEEIVRRLDPSKYPVPENWLHKEAQQLFLEPEVVDPESVELKWSEPNEEELVKFMCGEKQFSEERIRSGVKRLSKSRQGSTQGRLDDFFKVTGSLSSAKRKEPEPKGPAKKKAKTGGAGKFRRGK 827946BD8BDCEF39 apex1_human DNA-(apurinic or apyrimidinic site) endonuclease Apurinic-apyrimidinic endonuclease 1 APEX nuclease APE1 APEX1 APE APEX APX HAP1 REF1 REF-1 Redox factor-1 protein protein human Homo sapiens Human MPKRGKKGAVAEDGDELRTEPEAKKSKTAAKKNDKEAAGEGPALYEDPPDQKTSPSGKPATLKICSWNVDGLRAWIKKKGLDWVKEEAPDILCLQETKCSENKLPAELQELPGLSHQYWSAPSDKEGYSGVGLLSRQCPLKVSYGIGDEEHDQEGRVIVAEFDSFVLVTAYVPNAGRGLVRLEYRQRWDEAFRKFLKGLASRKPLVLCGDLNVAHEEIDLRNPKGNKKNAGFTPQERQGFGELLQAVPLADSFRHLYPNTPYAYTFWTYMMNARSKNVGWRLDYFLLSHSLLPALCDSKIRSKALGSDHCPITLYLAL DIP protein P27695 original sequence version: 139 DIP protein P27695 original sequence version: 152 DIP protein P27695 original sequence version: 187 B88579C01BAF80C6 polb-apex1-1 1 3 unspecified role unspecified role prey prey 7 unspecified role unspecified role prey prey 6 unspecified role unspecified role prey prey 4 unspecified role unspecified role bait bait nucl transfection nucleic acid transfection nucl transfection n-terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range association association 6A hmgb1-apex1-1 2 3 unspecified role unspecified role prey prey 7 unspecified role unspecified role prey prey 6 unspecified role unspecified role bait bait 4 unspecified role unspecified role prey prey nucl transfection nucleic acid transfection nucl transfection n-terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range association association 6B polb-apex1-2 2 8 unspecified role unspecified role prey prey purified purified ecoli Escherichia coli (strain K12) 5 unspecified role unspecified role prey prey purified purified human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 4 unspecified role unspecified role bait bait purified purified association association 6C FEN1 is found associated but cannot be mapped to UniProt hmgb1-apex1-2 2 8 unspecified role unspecified role prey prey purified purified ecoli Escherichia coli (strain K12) 5 unspecified role unspecified role bait bait purified purified human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 4 unspecified role unspecified role prey prey purified purified association association 6D FEN1 is found associated but cannot be mapped to UniProt