IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ A role for Erbin in the regulation of Nod2-dependent NF-kappaB signaling. A role for Erbin in the regulation of Nod2-dependent NF-kappaB signaling. The Journal of biological chemistry 2005 imex curation McDonald C., Chen FF., Ollendorff V., Ogura Y., Marchetto S., Lécine P., Borg JP., Nuñez G. 22-Feb-2016: Contacted by IntAct-Help. Only protein-protein interactions yeast Saccharomyces cerevisiae Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined McDonald C., Chen FF., Ollendorff V., Ogura Y., Marchetto S., Lécine P., Borg JP., Nuñez G. Accepted 2016-JAN-27 AT 09:40 GMT AT 09:40 GMT by ORCHARD The Journal of biological chemistry 2005 imex curation A role for Erbin in the regulation of Nod2-dependent NF-kappaB signaling. A role for Erbin in the regulation of Nod2-dependent NF-kappaB signaling. The Journal of biological chemistry 2005 imex curation McDonald C., Chen FF., Ollendorff V., Ogura Y., Marchetto S., Lécine P., Borg JP., Nuñez G. 22-Feb-2016: Contacted by IntAct-Help. Only protein-protein interactions human-lovo Homo sapiens colon adenocarcinoma cell line lovo lovo colon adenocarcinoma cell line lovo anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot McDonald C., Chen FF., Ollendorff V., Ogura Y., Marchetto S., Lécine P., Borg JP., Nuñez G. Accepted 2016-JAN-27 AT 09:40 GMT AT 09:40 GMT by ORCHARD The Journal of biological chemistry 2005 imex curation A role for Erbin in the regulation of Nod2-dependent NF-kappaB signaling. A role for Erbin in the regulation of Nod2-dependent NF-kappaB signaling. The Journal of biological chemistry 2005 imex curation McDonald C., Chen FF., Ollendorff V., Ogura Y., Marchetto S., Lécine P., Borg JP., Nuñez G. 22-Feb-2016: Contacted by IntAct-Help. Only protein-protein interactions human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti bait coip anti bait coimmunoprecipitation anti bait coip anti tag western anti tag western blot anti tag western McDonald C., Chen FF., Ollendorff V., Ogura Y., Marchetto S., Lécine P., Borg JP., Nuñez G. Accepted 2016-JAN-27 AT 09:40 GMT AT 09:40 GMT by ORCHARD The Journal of biological chemistry 2005 imex curation A role for Erbin in the regulation of Nod2-dependent NF-kappaB signaling. A role for Erbin in the regulation of Nod2-dependent NF-kappaB signaling. The Journal of biological chemistry 2005 imex curation McDonald C., Chen FF., Ollendorff V., Ogura Y., Marchetto S., Lécine P., Borg JP., Nuñez G. 22-Feb-2016: Contacted by IntAct-Help. Only protein-protein interactions human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot McDonald C., Chen FF., Ollendorff V., Ogura Y., Marchetto S., Lécine P., Borg JP., Nuñez G. Accepted 2016-JAN-27 AT 09:40 GMT AT 09:40 GMT by ORCHARD The Journal of biological chemistry 2005 imex curation A role for Erbin in the regulation of Nod2-dependent NF-kappaB signaling. A role for Erbin in the regulation of Nod2-dependent NF-kappaB signaling. The Journal of biological chemistry 2005 imex curation McDonald C., Chen FF., Ollendorff V., Ogura Y., Marchetto S., Lécine P., Borg JP., Nuñez G. 22-Feb-2016: Contacted by IntAct-Help. Only protein-protein interactions human-sw480 Human SW480 adenocarcinoma cell line sw480 SW480- human primary colorectal adenocarcinoma cell line There is a G -> A mutation in codon 273 of the p53 gene resulting in an Arg -> His substitution and a C -> T mutation in codon 309 resulting in a Pro -> Ser substitution. http://www.atcc.org/ATCCAdvancedCatalogSearch/ProductDetails/tabid/452/Default.aspx?ATCCNum=CCL-228&Template=cellBiology anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot McDonald C., Chen FF., Ollendorff V., Ogura Y., Marchetto S., Lécine P., Borg JP., Nuñez G. Accepted 2016-JAN-27 AT 09:40 GMT AT 09:40 GMT by ORCHARD The Journal of biological chemistry 2005 imex curation A role for Erbin in the regulation of Nod2-dependent NF-kappaB signaling. A role for Erbin in the regulation of Nod2-dependent NF-kappaB signaling. The Journal of biological chemistry 2005 imex curation McDonald C., Chen FF., Ollendorff V., Ogura Y., Marchetto S., Lécine P., Borg JP., Nuñez G. 22-Feb-2016: Contacted by IntAct-Help. Only protein-protein interactions human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip sequence tag sequence tag identification sequence tag McDonald C., Chen FF., Ollendorff V., Ogura Y., Marchetto S., Lécine P., Borg JP., Nuñez G. Accepted 2016-JAN-27 AT 09:40 GMT AT 09:40 GMT by ORCHARD The Journal of biological chemistry 2005 imex curation A role for Erbin in the regulation of Nod2-dependent NF-kappaB signaling. A role for Erbin in the regulation of Nod2-dependent NF-kappaB signaling. The Journal of biological chemistry 2005 imex curation McDonald C., Chen FF., Ollendorff V., Ogura Y., Marchetto S., Lécine P., Borg JP., Nuñez G. 22-Feb-2016: Contacted by IntAct-Help. Only protein-protein interactions human-caco2 Human Caucasian colon adenocarcinoma caco2 Human Caucasian colon adenocarcinoma confocal microscopy confocal microscopy immunostaining immunostaining McDonald C., Chen FF., Ollendorff V., Ogura Y., Marchetto S., Lécine P., Borg JP., Nuñez G. Accepted 2016-JAN-27 AT 09:40 GMT AT 09:40 GMT by ORCHARD The Journal of biological chemistry 2005 imex curation A role for Erbin in the regulation of Nod2-dependent NF-kappaB signaling. A role for Erbin in the regulation of Nod2-dependent NF-kappaB signaling. The Journal of biological chemistry 2005 imex curation McDonald C., Chen FF., Ollendorff V., Ogura Y., Marchetto S., Lécine P., Borg JP., Nuñez G. 22-Feb-2016: Contacted by IntAct-Help. Only protein-protein interactions human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western McDonald C., Chen FF., Ollendorff V., Ogura Y., Marchetto S., Lécine P., Borg JP., Nuñez G. Accepted 2016-JAN-27 AT 09:40 GMT AT 09:40 GMT by ORCHARD The Journal of biological chemistry 2005 imex curation q96rt1-2 Erbin ERBIN Densin-180-like protein ERBB2IP Erbb2-interacting protein KIAA1225 LAP2 Protein LAP2 protein protein human Homo sapiens Human MTTKRSLFVRLVPCRCLRGEEETVTTLDYSHCSLEQVPKEIFTFEKTLEELYLDANQIEELPKQLFNCQSLHKLSLPDNDLTTLPASIANLINLRELDVSKNGIQEFPENIKNCKVLTIVEASVNPISKLPDGFSQLLNLTQLYLNDAFLEFLPANFGRLTKLQILELRENQLKMLPKTMNRLTQLERLDLGSNEFTEVPEVLEQLSGLKEFWMDANRLTFIPGFIGSLKQLTYLDVSKNNIEMVEEGISTCENLQDLLLSSNSLQQLPETIGSLKNITTLKIDENQLMYLPDSIGGLISVEELDCSFNEVEALPSSIGQLTNLRTFAADHNYLQQLPPEIGSWKNITVLFLHSNKLETLPEEMGDMQKLKVINLSDNRLKNLPFSFTKLQQLTAMWLSDNQSKPLIPLQKETDSETQKMVLTNYMFPQQPRTEDVMFISDNESFNPSLWEEQRKQRAQVAFECDEDKDEREAPPREGNLKRYPTPYPDELKNMVKTVQTIVHRLKDEETNEDSGRDLKPHEDQQDINKDVGVKTSESTTTVKSKVDEREKYMIGNSVQKISEPEAEISPGSLPVTANMKASENLKHIVNHDDVFEESEELSSDEEMKMAEMRPPLIETSINQPKVVALSNNKKDDTKETDSLSDEVTHNSNQNNSNCSSPSRMSDSVSLNTDSSQDTSLCSPVKQTHIDINSKIRQEDENFNSLLQNGDILNSSTEEKFKAHDKKDFNLPEYDLNVEERLVLIEKSVDSTATADDTHKLDHINMNLNKLITNDTFQPEIMERSKTQDIVLGTSFLSINSKEETEHLENGNKYPNLESVNKVNGHSEETSQSPNRTEPHDSDCSVDLGISKSTEDLSPQKSGPVGSVVKSHSITNMEIGGLKIYDILSDNGPQQPSTTVKITSAVDGKNIVRSKSATLLYDQPLQVFTGSSSSSDLISGTKAIFKFDSNHNPEEPNIIRGPTSGPQSAPQIYGPPQYNIQYSSSAAVKDTLWHSKQNPQIDHASFPPQLLPRSESTENQSYAKHSANMNFSNHNNVRANTAYHLHQRLGPARHGEMWAISPNDRLIPAVTRSTIQRQSSVSSTASVNLGDPGSTRRAQIPEGDYLSYREFHSAGRTPPMMPGSQRPLSARTYSIDGPNASRPQSARPSINEIPERTMSVSDFNYSRTSPSKRPNARVGSEHSLLDPPGKSKVPRDWREQVLRHIEAKKLEKMPLSNGQMGQPLRPQANYSQIHHPPQASVARHPSREQLIDYLMLKVAHQPPYTQPHCSPRQGHELAKQEIRVRVEKDPELGFSISGGVGGRGNPFRPDDDGIFVTRVQPEGPASKLLQPGDKIIQANGYSFINIEHGQAVSLLKTFQNTVELIIVREVSS mint C8F24683B0C4324D erbin_human Erbin ERBIN Densin-180-like protein ERBB2IP Erbb2-interacting protein KIAA1225 LAP2 Protein LAP2 protein protein human Homo sapiens Human MTTKRSLFVRLVPCRCLRGEEETVTTLDYSHCSLEQVPKEIFTFEKTLEELYLDANQIEELPKQLFNCQSLHKLSLPDNDLTTLPASIANLINLRELDVSKNGIQEFPENIKNCKVLTIVEASVNPISKLPDGFSQLLNLTQLYLNDAFLEFLPANFGRLTKLQILELRENQLKMLPKTMNRLTQLERLDLGSNEFTEVPEVLEQLSGLKEFWMDANRLTFIPGFIGSLKQLTYLDVSKNNIEMVEEGISTCENLQDLLLSSNSLQQLPETIGSLKNITTLKIDENQLMYLPDSIGGLISVEELDCSFNEVEALPSSIGQLTNLRTFAADHNYLQQLPPEIGSWKNITVLFLHSNKLETLPEEMGDMQKLKVINLSDNRLKNLPFSFTKLQQLTAMWLSDNQSKPLIPLQKETDSETQKMVLTNYMFPQQPRTEDVMFISDNESFNPSLWEEQRKQRAQVAFECDEDKDEREAPPREGNLKRYPTPYPDELKNMVKTVQTIVHRLKDEETNEDSGRDLKPHEDQQDINKDVGVKTSESTTTVKSKVDEREKYMIGNSVQKISEPEAEISPGSLPVTANMKASENLKHIVNHDDVFEESEELSSDEEMKMAEMRPPLIETSINQPKVVALSNNKKDDTKETDSLSDEVTHNSNQNNSNCSSPSRMSDSVSLNTDSSQDTSLCSPVKQTHIDINSKIRQEDENFNSLLQNGDILNSSTEEKFKAHDKKDFNLPEYDLNVEERLVLIEKSVDSTATADDTHKLDHINMNLNKLITNDTFQPEIMERSKTQDIVLGTSFLSINSKEETEHLENGNKYPNLESVNKVNGHSEETSQSPNRTEPHDSDCSVDLGISKSTEDLSPQKSGPVGSVVKSHSITNMEIGGLKIYDILSDNGPQQPSTTVKITSAVDGKNIVRSKSATLLYDQPLQVFTGSSSSSDLISGTKAIFKFDSNHNPEEPNIIRGPTSGPQSAPQIYGPPQYNIQYSSSAAVKDTLWHSKQNPQIDHASFPPQLLPRSESTENQSYAKHSANMNFSNHNNVRANTAYHLHQRLGPARHGEMWAISPNDRLIPAVTRSTIQRQSSVSSTASVNLGDPGSTRRAQIPEGDYLSYREFHSAGRTPPMMPGSQRPLSARTYSIDGPNASRPQSARPSINEIPERTMSVSDFNYSRTSPSKRPNARVGSEHSLLDPPGKSKVPRDWREQVLRHIEAKKLEKKHPQTSSSGDPCQDGIFISGQQNYSSATLSHKDVPPDSLMKMPLSNGQMGQPLRPQANYSQIHHPPQASVARHPSREQLIDYLMLKVAHQPPYTQPHCSPRQGHELAKQEIRVRVEKDPELGFSISGGVGGRGNPFRPDDDGIFVTRVQPEGPASKLLQPGDKIIQANGYSFINIEHGQAVSLLKTFQNTVELIIVREVSS 304DFC81578CF671 pkp4_human Plakophilin-4 p0071 PKP4 protein protein human Homo sapiens Human MPAPEQASLVEEGQPQTRQEAASTGPGMEPETTATTILASVKEQELQFQRLTRELEVERQIVASQLERCRLGAESPSIASTSSTEKSFPWRSTDVPNTGVSKPRVSDAVQPNNYLIRTEPEQGTLYSPEQTSLHESEGSLGNSRSSTQMNSYSDSGYQEAGSFHNSQNVSKADNRQQHSFIGSTNNHVVRNSRAEGQTLVQPSVANRAMRRVSSVPSRAQSPSYVISTGVSPSRGSLRTSLGSGFGSPSVTDPRPLNPSAYSSTTLPAARAASPYSQRPASPTAIRRIGSVTSRQTSNPNGPTPQYQTTARVGSPLTLTDAQTRVASPSQGQVGSSSPKRSGMTAVPQHLGPSLQRTVHDMEQFGQQQYDIYERMVPPRPDSLTGLRSSYASQHSQLGQDLRSAVSPDLHITPIYEGRTYYSPVYRSPNHGTVELQGSQTALYRTGSVGIGNLQRTSSQRSTLTYQRNNYALNTTATYAEPYRPIQYRVQECNYNRLQHAVPADDGTTRSPSIDSIQKDPREFAWRDPELPEVIHMLQHQFPSVQANAAAYLQHLCFGDNKVKMEVCRLGGIKHLVDLLDHRVLEVQKNACGALRNLVFGKSTDENKIAMKNVGGIPALLRLLRKSIDAEVRELVTGVLWNLSSCDAVKMTIIRDALSTLTNTVIVPHSGWNNSSFDDDHKIKFQTSLVLRNTTGCLRNLSSAGEEARKQMRSCEGLVDSLLYVIHTCVNTSDYDSKTVENCVCTLRNLSYRLELEVPQARLLGLNELDDLLGKESPSKDSEPSCWGKKKKKKKRTPQEDQWDGVGPIPGLSKSPKGVEMLWHPSVVKPYLTLLAESSNPATLEGSAGSLQNLSAGNWKFAAYIRAAVRKEKGLPILVELLRMDNDRVVSSVATALRNMALDVRNKELIGKYAMRDLVNRLPGGNGPSVLSDETMAAICCALHEVTSKNMENAKALADSGGIEKLVNITKGRGDRSSLKVVKAAAQVLNTLWQYRDLRSIYKKDGWNQNHFITPVSTLERDRFKSHPSLSTTNQQMSPIIQSVGSTSSSPALLGIRDPRSEYDRTQPPMQYYNSQGDATHKGLYPGSSKPSPIYISSYSSPAREQNRRLQHQQLYYSQDDSNRKNFDAYRLYLQSPHSYEDPYFDDRVHFPASTDYSTQYGLKSTTNYVDFYSTKRPSYRAEQYPGSPDSWV 48EA1579E323E019 nod2_human Nucleotide-binding oligomerization domain-containing protein 2 IBD1 NOD2 CARD15 Caspase recruitment domain-containing protein 15 Inflammatory bowel disease protein 1 protein protein human Homo sapiens Human MGEEGGSASHDEEERASVLLGHSPGCEMCSQEAFQAQRSQLVELLVSGSLEGFESVLDWLLSWEVLSWEDYEGFHLLGQPLSHLARRLLDTVWNKGTWACQKLIAAAQEAQADSQSPKLHGCWDPHSLHPARDLQSHRPAIVRRLHSHVENMLDLAWERGFVSQYECDEIRLPIFTPSQRARRLLDLATVKANGLAAFLLQHVQELPVPLALPLEAATCKKYMAKLRTTVSAQSRFLSTYDGAETLCLEDIYTENVLEVWADVGMAGPPQKSPATLGLEELFSTPGHLNDDADTVLVVGEAGSGKSTLLQRLHLLWAAGQDFQEFLFVFPFSCRQLQCMAKPLSVRTLLFEHCCWPDVGQEDIFQLLLDHPDRVLLTFDGFDEFKFRFTDRERHCSPTDPTSVQTLLFNLLQGNLLKNARKVVTSRPAAVSAFLRKYIRTEFNLKGFSEQGIELYLRKRHHEPGVADRLIRLLQETSALHGLCHLPVFSWMVSKCHQELLLQEGGSPKTTTDMYLLILQHFLLHATPPDSASQGLGPSLLRGRLPTLLHLGRLALWGLGMCCYVFSAQQLQAAQVSPDDISLGFLVRAKGVVPGSTAPLEFLHITFQCFFAAFYLALSADVPPALLRHLFNCGRPGNSPMARLLPTMCIQASEGKDSSVAALLQKAEPHNLQITAAFLAGLLSREHWGLLAECQTSEKALLRRQACARWCLARSLRKHFHSIPPAAPGEAKSVHAMPGFIWLIRSLYEMQEERLARKAARGLNVGHLKLTFCSVGPTECAALAFVLQHLRRPVALQLDYNSVGDIGVEQLLPCLGVCKALYLRDNNISDRGICKLIECALHCEQLQKLALFNNKLTDGCAHSMAKLLACRQNFLALRLGNNYITAAGAQVLAEGLRGNTSLQFLGFWGNRVGDEGAQALAEALGDHQSLRWLSLVGNNIGSVGAQALALMLAKNVMLEELCLEENHLQDEGVCSLAEGLKKNSSLKILKLSNNCITYLGAEALLQALERNDTILEVWLRGNTFSLEEVDKLGCRDTRLLL DIP protein Q9HC29 original sequence version: 141 DIP protein Q9HC29 original sequence version: 77 DIP protein Q9HC29-1 original sequence version: 100 DIP protein Q9HC29-1 original sequence version: 106 mint homomint Induces NF-kappa-B via RICK (CARDIAK, RIP2) and IKK-gamma. Confers responsiveness to intracellular bacterial lipopolysaccharides (LPS) Defects in NOD2 are the cause of Blau syndrome (BS) [MIM:186580]. BS is a rare autosomal dominant disorder characterized by early-onset granulomatous arthritis, uveitis and skin rash Defects in NOD2 are a cause of susceptibility to Crohn disease (CD) [MIM:266600]. CD is a form of remitting inflammatory bowel disease (IBD). CD may involve any part of the gastrointestinal tract, but most frequently the terminal ileum and colon. Bowel inflammation is transmural and discontinuous. Crohn disease is commonly classified as autoimmune disease Defects in NOD2 are a cause of susceptibility to ulcerative colitis [MIM:191390]. It is a chronic inflammatory bowel disease. In ulcerative colitis, the inflammation is continuous and limited to rectal and colonic mucosal layers. Ulcerative colitis is commonly classified as autoimmune disease Defects in NOD2 are the cause of early-onset sarcoidosis (EOS) [MIM:609464]. Sarcoidosis is a multiorganic inflammatory disease with unknown etiology, characterized by the histologic features of non-caseating epithelioid granulomas. In childhood, 2 distinct types of sarcoidosis have been described. Usually the disease is detected in older children by chest radiography and the clinical manifestations are characterized by a classical triad of lung, lymph node, and eye involvement, similar to those in adults. In contrast, early-onset sarcoidosis (EOS), which usually appears in those younger than 4 years of age, is quite rare and has a distinct triad of skin, joint, and eye disorders, without apparent pulmonary involvement. Compared with an asymptomatic and sometimes naturally disappearing course of the disease in older children, EOS is progressive and in many cases causes severe complications, such as blindness, joint destruction, and visceral involvement 0037592D96D7DDFF nod2-1 1 12 unspecified role unspecified role prey prey 9 unspecified role unspecified role bait bait physical association physical association Fig. 1D nod2-8 4 12 unspecified role unspecified role bait bait ?-? flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 10 unspecified role unspecified role prey prey physical association physical association Fig. 1B erbb2ip-nod2-2 5 9 unspecified role unspecified role prey prey 12 unspecified role unspecified role bait bait physical association physical association Fig. 1C erbb2ip-nod2-3 7 12 unspecified role unspecified role neutral component neutral component ?-? egfp tag enhanced green fluorescent protein tag enhanced green fluorescent protein EGFP enhanced green fluorescent protein EGFP egfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 10 unspecified role unspecified role neutral component neutral component colocalization colocalization Fig. 1E nod2-erbb2ip-4 8 12 unspecified role unspecified role bait bait Q9HC29:p.Arg702Trp mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain R W Wed May 02 17:46:34 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg702trp' Tue Jun 30 16:43:02 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg702Trp' Q9HC29:p.Gly908Arg mutation increasing mutation increasing interaction mutation increasing certain certain sequence position certain certain certain sequence position certain G R Wed May 02 16:17:35 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gly908arg' Tue Jun 30 19:53:49 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly908Arg' region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain Q9HC29:p.Arg334Trp mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain R W Wed May 02 18:05:58 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg334trp' Tue Jun 30 16:13:05 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg334Trp' Q9HC29:p.Leu1007Cys mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain L C Wed May 02 18:12:05 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu1007cys' Tue Jun 30 19:04:08 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu1007Cys' Q9HC29:p.His496Phe mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain H F Fri Jul 22 11:11:44 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'leu496phe' Wed May 02 15:29:57 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'his496phe' Tue Jun 30 17:49:47 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.His496Phe' region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 9 unspecified role unspecified role prey prey region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Fig. 2, 3A, 4A erbb2ip-nod2-1 3 9 unspecified role unspecified role prey prey region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 12 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Fig. 3A (left top panel) erbb2ip-nod2-4 8 9 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain Q96RT1-2:p.Pro315Leu mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain P L Wed May 02 19:33:37 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'pro315leu' Tue Jun 30 19:47:58 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Pro315Leu' region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain Q96RT1-2:p.His1347_Gly1348delinsTyrAsp mutation mutation certain certain sequence position certain certain certain sequence position certain HG YD Wed May 02 16:34:44 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'his_gly1347-1348tyr_asp' Tue Jun 30 16:32:27 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.His1347_Gly1348delinsTyrAsp' 12 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Fig. 3 B (top blot) nod2-erbb2ip-1 2 12 unspecified role unspecified role bait bait 10 unspecified role unspecified role prey prey physical association physical association Fig. 6E pkp4-1 1 9 unspecified role unspecified role bait bait 11 unspecified role unspecified role prey prey physical association physical association Fig. 1 D nod2-erbb2ip-3 6 12 unspecified role unspecified role bait bait ?-? flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 10 unspecified role unspecified role prey prey physical association physical association Fig. 1A