UniProt UniProt http://www.uniprot.org QRX, a novel homeobox gene, modulates photoreceptor gene expression. QRX, a novel homeobox gene, modulates photoreceptor gene expression. Human molecular genetics 2004 imex curation Wang QL., Chen S., Esumi N., Swain PK., Haines HS., Peng G., Melia BM., McIntosh I., Heckenlively JR., Jacobson SG., Stone EM., Swaroop A., Zack DJ. Expression of recombinant proteins (IP fig 6A) according Ref. 73, De Spada et al (2001) PubMed:11580893. Origin of Crx accord. Ref 12, Chen et al (1997) PubMed:9390516. Origin of NRL accord. Ref 32, Mitton et al (2000) PubMed:10887186. dzak@bs.jhmi.edu 17-Sep-2015: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro pull down pull down weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Wang QL., Chen S., Esumi N., Swain PK., Haines HS., Peng G., Melia BM., McIntosh I., Heckenlively JR., Jacobson SG., Stone EM., Swaroop A., Zack DJ. Expression of recombinant proteins (IP fig 6A) according Ref. 73, De Spada et al (2001) PubMed:11580893. Origin of Crx accord. Ref 12, Chen et al (1997) PubMed:9390516. Origin of NRL accord. Ref 32, Mitton et al (2000) PubMed:10887186. dzak@bs.jhmi.edu Accepted 2015-JUL-15 by ORCHARD Human molecular genetics 2004 imex curation mimix curation QRX, a novel homeobox gene, modulates photoreceptor gene expression. QRX, a novel homeobox gene, modulates photoreceptor gene expression. Human molecular genetics 2004 imex curation Wang QL., Chen S., Esumi N., Swain PK., Haines HS., Peng G., Melia BM., McIntosh I., Heckenlively JR., Jacobson SG., Stone EM., Swaroop A., Zack DJ. Expression of recombinant proteins (IP fig 6A) according Ref. 73, De Spada et al (2001) PubMed:11580893. Origin of Crx accord. Ref 12, Chen et al (1997) PubMed:9390516. Origin of NRL accord. Ref 32, Mitton et al (2000) PubMed:10887186. dzak@bs.jhmi.edu 17-Sep-2015: Contacted by IntAct-Help. Only protein-protein interactions human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Wang QL., Chen S., Esumi N., Swain PK., Haines HS., Peng G., Melia BM., McIntosh I., Heckenlively JR., Jacobson SG., Stone EM., Swaroop A., Zack DJ. Expression of recombinant proteins (IP fig 6A) according Ref. 73, De Spada et al (2001) PubMed:11580893. Origin of Crx accord. Ref 12, Chen et al (1997) PubMed:9390516. Origin of NRL accord. Ref 32, Mitton et al (2000) PubMed:10887186. dzak@bs.jhmi.edu Accepted 2015-JUL-15 by ORCHARD Human molecular genetics 2004 IMEx imex curation QRX, a novel homeobox gene, modulates photoreceptor gene expression. QRX, a novel homeobox gene, modulates photoreceptor gene expression. Human molecular genetics 2004 imex curation Wang QL., Chen S., Esumi N., Swain PK., Haines HS., Peng G., Melia BM., McIntosh I., Heckenlively JR., Jacobson SG., Stone EM., Swaroop A., Zack DJ. Expression of recombinant proteins (IP fig 6A) according Ref. 73, De Spada et al (2001) PubMed:11580893. Origin of Crx accord. Ref 12, Chen et al (1997) PubMed:9390516. Origin of NRL accord. Ref 32, Mitton et al (2000) PubMed:10887186. dzak@bs.jhmi.edu 17-Sep-2015: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro anti bait coip anti bait coimmunoprecipitation anti bait coip weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Wang QL., Chen S., Esumi N., Swain PK., Haines HS., Peng G., Melia BM., McIntosh I., Heckenlively JR., Jacobson SG., Stone EM., Swaroop A., Zack DJ. Expression of recombinant proteins (IP fig 6A) according Ref. 73, De Spada et al (2001) PubMed:11580893. Origin of Crx accord. Ref 12, Chen et al (1997) PubMed:9390516. Origin of NRL accord. Ref 32, Mitton et al (2000) PubMed:10887186. dzak@bs.jhmi.edu Accepted 2015-JUL-15 by ORCHARD Human molecular genetics 2004 IMEx imex curation crx_bovin Cone-rod homeobox protein CRX protein protein bovin Bos taurus (Bovine) Bovine MMAYMNPGPHYSVNALALSGPSVDLMHPAVSYPSAPRKQRRERTTFTRSQLEELEALFAKTQYPDVYAREEVALKINLPESRVQVWFKNRRAKCRQQRQQQKQQQQPPGAQAKARPAKRKAGTSPRSSTDVCPDPLGISDSYSPPLPGPSGSPTTAVATVSIWSPASESPLPEAQRAGLVASGPSLTSAPYAMTYAPASAFCSSPSAYGSPSSYFSGLDPYLSPMVPPLGGPALSPLSGPSVGPSLTQSPTSLSGQSYGTYSPVDSLEFKDPTGTWKFTYNPMDPLDYKDQSAWKFQIL 512ED6A6DAFBAC19 rax2_human Retina and anterior neural fold homeobox protein 2 RAX2 QRX RAXL1 Q50-type retinal homeobox protein Retina and anterior neural fold homeobox-like protein 1 protein protein human Homo sapiens Human MFLSPGEGPATEGGGLGPGEEAPKKKHRRNRTTFTTYQLHQLERAFEASHYPDVYSREELAAKVHLPEVRVQVWFQNRRAKWRRQERLESGSGAVAAPRLPEAPALPFARPPAMSLPLEPWLGPGPPAVPGLPRLLGPGPGLQASFGPHAFAPTFADGFALEEASLRLLAKEHAQALDRAWPPA DE2FC85C4A4DE063 nrl_human Neural retina-specific leucine zipper protein NRL D14S46E protein protein human Homo sapiens Human MALPPSPLAMEYVNDFDLMKFEVKREPSEGRPGPPTASLGSTPYSSVPPSPTFSEPGMVGATEGTRPGLEELYWLATLQQQLGAGEALGLSPEEAMELLQGQGPVPVDGPHGYYPGSPEETGAQHVQLAERFSDAALVSMSVRELNRQLRGCGRDEALRLKQRRRTLKNRGYAQACRSKRLQQRRGLEAERARLAAQLDALRAEVARLARERDLYKARCDRLTSSGPGSGDPSHLFL CCABEDC1C1123614 rax2_bovin Retina and anterior neural fold homeobox protein 2 RAX2 QRX RAXL1 Q50-type retinal homeobox protein Retina and anterior neural fold homeobox-like protein 1 protein protein bovin Bos taurus (Bovine) Bovine MFLSPGEGPAAEGGGPGPGEEAPKKKHRRNRTTFTTYQLHQLERAFEASHYPDVYSREELAAKVHLPEVRVQVWFQNRRAKWRRQERLESGSGAVAAPRLPEVPALPFARPPTMPLSLEPWLGPGPPAVPALPRLLGSGPGLQGSFGPHAFSPAFMDGFTLEEGSLRLLAKEHVQALDRAWPSA F94082F544880A2E rx_mouse Retinal homeobox protein Rx Rax Rx Retina and anterior neural fold homeobox protein protein protein mouse Mus musculus Mouse MHLPGCAPAMADGSFSLAGHLLRSPGGSTSRLHSIEAILGFTKEDGILDTFPAERSSRSSKERDPRLGAQPACPKAPAGGSESSPPAAPGFVPEYEATRPCYPKEQGEARPSPGLSVGPAAGDSKLSEEEPPKKKHRRNRTTFTTYQLHELERAFEKSHYPDVYSREELAGKVNLPEVRVQVWFQNRRAKWRRQEKLEVSSMKLQDSPLLSFSRSPPSSALAPLGTGPGSGSGPPGSALPLEPWLGPPLPGGGATALQSLPGFGPPGQGLPASYTPPPPFLNSAPLGPGLQQLGPPPAYPCAPAFGDKFSLEEAYPRNSSIAALRLKAKEHIQAIGKPWQAL 8315DAF6B038D087 crx_human Cone-rod homeobox protein CRX CORD2 protein protein human Homo sapiens Human MMAYMNPGPHYSVNALALSGPSVDLMHQAVPYPSAPRKQRRERTTFTRSQLEELEALFAKTQYPDVYAREEVALKINLPESRVQVWFKNRRAKCRQQRQQQKQQQQPPGGQAKARPAKRKAGTSPRPSTDVCPDPLGISDSYSPPLPGPSGSPTTAVATVSIWSPASESPLPEAQRAGLVASGPSLTSAPYAMTYAPASAFCSSPSAYGSPSSYFSGLDPYLSPMVPQLGGPALSPLSGPSVGPSLAQSPTSLSGQSYGAYSPVDSLEFKDPTGTWKFTYNPMDPLDYKDQSAWKFQIL 46747C09277A0864 crx-rax2-1 3 7 unspecified role unspecified role prey prey in vitro translated in vitro translated protein in vitro translated region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined homeobox domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain Q7YRX0:p.Gly137Arg mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain G R Corresponds to human G137R disease variant in CORD11 Wed May 02 19:26:58 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gly137arg' Tue Jun 30 19:18:51 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly137Arg' Q7YRX0:p.Ser138_Gly139insGlyPro mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain SG SGPG Wed May 02 19:00:26 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gly139gly_pro_gly' Tue Jun 30 15:58:34 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser138_Gly139insGlyPro' rabit-reticulocyte Oryctolagus cuniculus reticulocyte reticulocyte Reticulocytes Mutation R87Q corresponding to human disease variant R87Q in ARMD6 is interacting 4 unspecified role unspecified role bait bait in vitro translated in vitro translated protein in vitro translated homeobox domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain rabit-reticulocyte Oryctolagus cuniculus reticulocyte reticulocyte Reticulocytes 17 13 physical association physical association 6A crx-rax2-2 2 5 unspecified role unspecified role prey prey nucl transfection nucleic acid transfection nucl transfection region xpress tag xpress tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 9 unspecified role unspecified role bait bait nucl transfection nucleic acid transfection nucl transfection region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 6B nrl-crx-4 1 4 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined homeobox domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain rabit-reticulocyte Oryctolagus cuniculus reticulocyte reticulocyte Reticulocytes 6 unspecified role unspecified role bait bait N/A gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 29 26 physical association physical association 6C nrl-rax-1 1 8 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined homeobox domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain rabit-reticulocyte Oryctolagus cuniculus reticulocyte reticulocyte Reticulocytes 6 unspecified role unspecified role bait bait N/A gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 36 33 physical association physical association 6C