matrixdb Institut de Biologie et de Chimie des Prot\u00c3\u00a9ines, 7, passage du Vercors 69 367 Lyon cedex 07 FRANCE matrixdb@ibcp.fr http://matrixdb.ibcp.fr Mock publication and experiment for abstract interactions that are not interaction evidences. Mock publication and experiment for abstract interactions that are not interaction evidences. unspecified method Type XXIII collagen, a new transmembrane collagen identified in metastatic tumor cells. Type XXIII collagen, a new transmembrane collagen identified in metastatic tumor cells. J. Biol. Chem. (0021-9258) 2003 imex curation Banyard J., Bao L., Zetter B.R. bruce.zetter@tch.harvard.edu 15-Sep-2016: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro affinity chrom affinity chromatography technology Affinity purification Affinity purification affinity chrom anti tag western anti tag western blot anti tag western Banyard J., Bao L., Zetter B.R. bruce.zetter@tch.harvard.edu Accepted 2015-FEB-02 by ORCHARD J. Biol. Chem. (0021-9258) 2003 Only protein-protein interactions imex curation Type XXIII collagen, a new transmembrane collagen identified in metastatic tumor cells. Type XXIII collagen, a new transmembrane collagen identified in metastatic tumor cells. J. Biol. Chem. (0021-9258) 2003 imex curation Banyard J., Bao L., Zetter B.R. bruce.zetter@tch.harvard.edu 15-Sep-2016: Contacted by IntAct-Help. Only protein-protein interactions rat-r3327 Dunning rat prostate tumour cells r3327 Dunning rat prostate tumour cells http://www.atcc.org Catalog #JHU-92 electrophoresis electrophoretic mobility-based method electrophoresis anti tag western anti tag western blot anti tag western Banyard J., Bao L., Zetter B.R. bruce.zetter@tch.harvard.edu Accepted 2015-FEB-02 by ORCHARD J. Biol. Chem. (0021-9258) 2003 Only protein-protein interactions imex curation cona1_human Collagen alpha-1(XXIII) chain COL23A1 protein protein human Homo sapiens Human MGPGERAGGGGDAGKGNAAGGGGGGRSATTAGSRAVSALCLLLSVGSAAACLLLGVQAAALQGRVAALEEERELLRRAGPPGALDAWAEPHLERLLREKLDGLAKIRTAREAPSECVCPPGPPGRRGKPGRRGDPGPPGQSGRDGYPGPLGLDGKPGLPGPKGEKGAPGDFGPRGDQGQDGAAGPPGPPGPPGARGPPGDTGKDGPRGAQGPAGPKGEPGQDGEMGPKGPPGPKGEPGVPGKKGDDGTPSQPGPPGPKGEPGSMGPRGENGVDGAPGPKGEPGHRGTDGAAGPRGAPGLKGEQGDTVVIDYDGRILDALKGPPGPQGPPGPPGIPGAKGELGLPGAPGIDGEKGPKGQKGDPGEPGPAGLKGEAGEMGLSGLPGADGLKGEKGESASDSLQESLAQLIVEPGPPGPPGPPGPMGLQGIQGPKGLDGAKGEKGASGERGPSGLPGPVGPPGLIGLPGTKGEKGRPGEPGLDGFPGPRGEKGDRSERGEKGERGVPGRKGVKGQKGEPGPPGLDQPCPVGPDGLPVPGCWHK D64D3CA50F729297 cona1_rat Collagen alpha-1(XXIII) chain Col23a1 protein protein rat Rattus norvegicus (Rat) Rat MGAGERAAGGGGTQDPGAGCGARALGALCLLLSVGSATACLLLGAQAAALHGRVAALEQERELLRRAGPSGALAAWAETHLERLLREKLDGVAKLRTVREAPSECICPPGPPGRRGKPGRRGDPGPPGQSGRDGYPGPLGLDGKPGLPGPKGEKGAPGDFGPRGAQGQDGAAGPPGPPGPPGARGPPGDTGKDGPRGAQGPEGPRGESGQDGEMGPMGPPGPKGEPGTPGKKGDDGIPSQPGLPGPPGPKGEPGDVGPQGETGVDGAPGLKGEPGHPGTDGAIGPRGPPGLKGEQGDTVVIDYDGRILDALKGPPGPQGAPGPPGIPGAKGELGLPGAPGIDGEKGPKGPKGDPGEPGPAGPKGETGEMGLSGLPGADGPKGEKGESASDHLQESLAQIIVEPGPPGPPGPPGPMGLQGIQGPKGLDGAKGEKGASGERGPHGLPGPVGPPGLIGLPGTKGEKGRPGEPGLDGFPGPRGEKGDRSERGEKGERGVPGRKGVKGQKGEPGPPGLDQPCPVGPDGLPVPGCWHK 061E92AA7E8B76DF heparin Heparin small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) col23a1-col23a1-1 3 5 unspecified role unspecified role neutral component neutral component binding site sufficient to bind sufficient binding region sufficient to bind predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag predetermined featur predetermined feature predetermined featur n-term range n-terminal range n-term range n-term range n-terminal range n-term range tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag anti tag western anti tag western blot anti tag western undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Figure 9 col23a1-col23a1-2 3 5 unspecified role unspecified role neutral component neutral component tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag anti tag western anti tag western blot anti tag western undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag predetermined featur predetermined feature predetermined featur n-term range n-terminal range n-term range n-term range n-terminal range n-term range binding site sufficient to bind sufficient binding region sufficient to bind predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain physical association physical association Figure 9 heparin-collagen-1 2 19 unspecified role unspecified role prey prey tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag anti tag western anti tag western blot anti tag western c-term range C-terminal range c-term range c-term range C-terminal range c-term range binding site sufficient to bind sufficient binding region sufficient to bind predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag predetermined featur predetermined feature predetermined featur c-term range C-terminal range c-term range c-term range C-terminal range c-term range 6 unspecified role unspecified role bait bait physical association physical association Figure 10A collagen_xxiii_human Type XXIII collagen Type XXIII collagen COL23A1 trimer Collagen type XXIII trimer 4 unspecified role unspecified role direct interaction direct interaction human Homo sapiens Human stable complex stable complex biological system reconstruction evidence based on inference from background scientific knowledge used in manual assertion biological system reconstruction evidence based on inference from background scientific knowledge used in manual assertion Type II orientated transmembrane collagen. Homotrimer Triple-helices consisting of about 1000 amino acids per chain forming a coiled coil structure. Each polypeptide forms a left-handed helix in which every third residue, glycine, comes into the centre of the superhelix. created with intact-jami-dbscripts