IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Essential regions of Saccharomyces cerevisiae telomerase RNA: separate elements for Est1p and Est2p interaction. Essential regions of Saccharomyces cerevisiae telomerase RNA: separate elements for Est1p and Est2p interaction. Molecular and cellular biology 2002 imex curation Livengood AJ., Zaug AJ., Cech TR. CECHT@hhmi.org 21-10-2015: Contacted by IntAct-Help. Only protein-protein interactions imex curation yeast Saccharomyces cerevisiae Baker's yeast anti tag coip anti tag coimmunoprecipitation anti tag coip northern blot northern blot northen Livengood AJ., Zaug AJ., Cech TR. CECHT@hhmi.org Accepted 2015-OCT-12 AT 09:51 BST AT 09:51 BST by ORCHARD Molecular and cellular biology 2002 imex curation Essential regions of Saccharomyces cerevisiae telomerase RNA: separate elements for Est1p and Est2p interaction. Essential regions of Saccharomyces cerevisiae telomerase RNA: separate elements for Est1p and Est2p interaction. Molecular and cellular biology 2002 imex curation Livengood AJ., Zaug AJ., Cech TR. CECHT@hhmi.org 21-10-2015: Contacted by IntAct-Help. Only protein-protein interactions imex curation yeast Saccharomyces cerevisiae Baker's yeast affinity chrom affinity chromatography technology Affinity purification Affinity purification affinity chrom northern blot northern blot northen Livengood AJ., Zaug AJ., Cech TR. CECHT@hhmi.org Accepted 2015-OCT-12 AT 09:51 BST AT 09:51 BST by ORCHARD Molecular and cellular biology 2002 imex curation tlc1_yeast_rna Yeast integral telomerase RNA template component, TLC1 rna ribonucleic acid RNA rna yeast Saccharomyces cerevisiae Baker's yeast AAUAAAACUAGAGAGGAAGAUAGGUACCCUAUGAAAAUGUCAAUGGCUGUUGCGUUUGCUUAAUCGUAUUUUUUUUUUUUUCAGUCCGUGUUUUUUGUACAUUCUACGUUUGAGUUUUCCAUCAUGCAGGCCUCAGAAAUUUGGUAGGCACUCGAUGGUGAAGAGAUAGUGUCGGAUUUCGGAUUGAUCUUUCAGUUGAUAGCCUGCUGCUCUUUUCUUUUCCAAAGAAUUUCGAGUAUGCUGGUGUCAGUGUAGAUGCUUGUGUGUGCGCAAUUUGUGGUUUUUUAUUGUGUUUCUACUUAUAGAUGGCUAAAAUCUGAGUUUAGAAAAUGCAAACCGUAAAUUCUUAAACACUGCUAUUGCAUUUAGUUGCUAAAGCAGUGUUUUUGAACUUAUUCCUGUUAUUCCUUCUUCGUACCGAUCCUCUUCUCGACCUAACCUUUUAAUUACCAUGGGAAGCCUACCAUCACCACACCCACACACAAAUGUUACAGCUAAUUGUUUAUUAGCAAAGUUUGCACGAGUUCGCUGUUUAUUUUUUUCUCGUUUUCUUAUACCUAGUAUUUUUUCUGACACUGUUUAAGGUGACAGAAAAAAAGGAGUUUAAGUUAGAUUUGCAAACAGACGGUGCUAAGCGCUGUCACUUUAUGUCUAUCUUAUCGUUAACUCUGGAAAAAGAAAAAGGAAAAAGAACGUCAGGGAACAUGAGUAUAUAUAGAAAUGGUUUAUUCUAGUUUUUUCCGUUUUUUCAGUAGAUUUUUGCCUUUAAAAGAAUAAAUCCCACUACAAAAAGGUAAAAUAAAAAAUCUAUUCACUGAACUUACUGAUGAAAUUUCCAAAUGUGCCCCGUACAUCGAACGAUGUGACAGAGAAAAAUACGAGUAGGUAAAUAAGCCAAAAGGCAAGGGUGUCCUUUCUUAAGCAUCGGUUAGGUUUGCGGGCGAUCAGUAACUGAACAAUGACACAAGAUCAAGAACGUAAUUUGAGAUUUUUCAAGAUGGUUUUUUUAGGUAUCUAUUAAAACUACUUUGAUGAUCAAUACGGUAUUUUUGUCGCAUUAUUUUCCAAGCGGAAGGAACCGUGUGUUCAUUUUAUGAAUCUUGGUGUUGUAUUCACAGCUACUUCUCCUAAUGCCUUCGAUGCAUUUAGAUAAUUUUUGGAAACAUUUUUUUUCUUGAUGUAUAUUUUUUGUAUUGUAGAAAUCGCGCGUACUGUACUUGUAUAUCGCUUUAUAAGCGCUUUUAAUUGAUUGUUCAUGACGAGGAUAGGCGGAUAGGCGGAGGUAUGCCUCUUAAUAUUUA TLC1 RNA contains a template sequence that Est2p uses to add irregular repeats of TG(1-3) residues onto a DNA end; RNA template component of telomerase est1_yeast Telomere elongation protein EST1 EST1 YLR233C L8083.15 Ever shorter telomeres protein 1 protein protein yeast Saccharomyces cerevisiae Baker's yeast MDNEEVNEECMRLFFKNARAHLDKHLTSRLTCDENAYITFRCFLDGIHRKSTRFLEELLLKQENMYHNNNYERINDSVIPLVLKLLWLQIHEPTLQWFEHWFHDIMRLSNRRKFRVFRIFQKKMIQFFKITHRYYYDIIEHLCAKYDMNSVISNALFAKLNLMQYTDGLSTHEKIILNTSNPLTFSIVISLQRCVINLGSTHFYKTLLNKPSNKPKSVEGFEKSIRYLNIASLYLPAVGDTYFQRAKIYLITGKFSLYFFELVRGALVRIPSKCALNNLKDFILTPDFPERRRLMKKLAILVSKDLKGEKSFFEGQIVLQFLSIVEHTLVPQSWNASRASNCWLLKEHLQMAALKYHSGNINVILENLAATMGSFDLMFTTRKSKEQKNKLKYADLSERQVFFLDLSFDFIANIIDVVIKPSWQKNMEDFRYLAIIRLLMCWIKSYRSILQYTHRHRKFCTSFALLLNDLINSPLNCSGNIYSHRPKRSYLFREDIIFREFSCINFALTDFNDDYVYDSPDMINNIIGCPTLTKVLSPKEECVLRIRSIIFSGMKFLEKNDTGVIWNASKYKFDLISPNIKIKRQIALSEISSKINVKTQQERVVSSRKVEAKRDEQQRKRAGKIAVTELEKQFANVRRTKKLSPLPEKDGVSSELVKHAASRGRKTITGPLSSDFLSYPDEAIDADEDITVQVPDTPT 9CF552AFDA6BDEDE 9CF552AFDA6BDEDE est1_tlc-1 1 3 unspecified role unspecified role prey prey region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain 4 unspecified role unspecified role bait bait tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association Fig5 est1_tlc-2 2 3 unspecified role unspecified role prey prey region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain 4 unspecified role unspecified role bait bait tag protein a tag protein a tag protein A protein A n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association Fig6, Fig7