IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Chick optic lobe contains a developmentally regulated alpha2alpha5beta2 nicotinic receptor subtype. Chick optic lobe contains a developmentally regulated alpha2alpha5beta2 nicotinic receptor subtype. Mol. Pharmacol. (0026-895X) 2000 imex curation Balestra B., Vailati S., Moretti M., Hanke W., Clementi F., Gotti C. I have not curated any of the Epi-binding expt after discussion with Sandra. Epi is the ligand of many of the AchR subunits and therefore the interactions from cell extracts are not specific by simply detecting relative % Epi-binding after several different rounds of IP. gotti@csfic.mi.cnr.it 17-Sep-2015: Contacted by IntAct-Help. Only protein-protein interactions chick-optic_lobe chicken optic lobe optic_lobe optic lobe anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Balestra B., Vailati S., Moretti M., Hanke W., Clementi F., Gotti C. I have not curated any of the Epi-binding expt after discussion with Sandra. Epi is the ligand of many of the AchR subunits and therefore the interactions from cell extracts are not specific by simply detecting relative % Epi-binding after several different rounds of IP. Preys were detected with poly- and monoclonal antibodies against different parts of the proteins. The secondary antibodies were revealed with 125[I]-protein A. In cases where the secondary antibody was monoclonal, blots were incubated with anti-rat IgG for a further 2 hours prior to detection by 125[I]-protein A. gotti@csfic.mi.cnr.it Accepted 2015-JUL-09 by PPORRAS Mol. Pharmacol. (0026-895X) 2000 Only protein-protein interactions imex curation Chick optic lobe contains a developmentally regulated alpha2alpha5beta2 nicotinic receptor subtype. Chick optic lobe contains a developmentally regulated alpha2alpha5beta2 nicotinic receptor subtype. Mol. Pharmacol. (0026-895X) 2000 imex curation Balestra B., Vailati S., Moretti M., Hanke W., Clementi F., Gotti C. I have not curated any of the Epi-binding expt after discussion with Sandra. Epi is the ligand of many of the AchR subunits and therefore the interactions from cell extracts are not specific by simply detecting relative % Epi-binding after several different rounds of IP. gotti@csfic.mi.cnr.it 17-Sep-2015: Contacted by IntAct-Help. Only protein-protein interactions chick-retina Gallus gallus retina retinal tissue anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Balestra B., Vailati S., Moretti M., Hanke W., Clementi F., Gotti C. I have not curated any of the Epi-binding expt after discussion with Sandra. Epi is the ligand of many of the AchR subunits and therefore the interactions from cell extracts are not specific by simply detecting relative % Epi-binding after several different rounds of IP. The secondary antibodies were revealed with 125[I]-protein A. In cases where the secondary antibody is monoclonal, blots were incubated with anti-rat IgG for a further 2 hours prior to development. gotti@csfic.mi.cnr.it Accepted 2015-JUL-09 by PPORRAS Mol. Pharmacol. (0026-895X) 2000 Only protein-protein interactions imex curation acha3_chick Neuronal acetylcholine receptor subunit alpha-3 CHRNA3 protein protein chick Gallus gallus (Chicken) Chicken MQSIHALLLTAAVCILFQGCGGSEPEHRLYAALFKNYNQFVRPVKNASDPVIIQFEVSMSQLVKVDEVNQIMETNLWLKHIWNDYKLRWNPVDYGGAEFIRVPSGQIWKPDIVLYNNAVGDFQVDDKTKALLKYTGDVTWIPPAIFKSSCKIDVTYFPFDYQNCTMKFGSWSYDKAKIDLVLIGSTMNLKDYWESGEWAIIKAPGYKHDIKYNCCEEIYTDITYSLYIRRLPLFYTINMIIPCLLISFLTVLVFYLPSDCGEKVTLCISVLLSLTVFLLVITETIPSTSLVIPLIGEYLLFTMIFVTLSIVITVFVLNVHYRTPKTHTMPVWVRTIFLNLLPRIMFMTRPTSDEENNQKPKPFYTSEFSNLNCFNSSEIKCCKDGFVCQDMACSCCQYQRMKFSDFSGNLTRSSSSESVDPLFSFSVLSPEMRDAIESVKYIAENMKMQNEAKEIQDDWKYVAMVIDRIFLWVFILVCILGTAGLFLQPLMTGDDM FD25BC02A9B601FF acha2_chick Neuronal acetylcholine receptor subunit alpha-2 CHRNA2 protein protein chick Gallus gallus (Chicken) Chicken MGWPCRSIIPLLVWCFVTLQAATREQKQPHGFAEDRLFKHLFTGYNRWSRPVPNTSDVVIVKFGLSIAQLIDVDEKNQMMTTNVWLKQEWSDYKLRWNPEDFDNVTSIRVPSEMIWIPDIVLYNNADGEFAVTHMTKAHLFSNGKVKWVPPAIYKSSCSIDVTYFPFDQQNCKMKFGSWTYDKAKIDLENMEHHVDLKDYWESGEWAIINAIGRYNSKKYDCCTEIYPDITFYFVIRRLPLFYTINLIIPCLLISCLTVLVFYLPSDCGEKITLCISVLLSLTVFLLLITEIIPSTSLVIPLIGEYLLFTMIFVTLSIIITVFVLNVHHRSPSTHTMPHWVRSFFLGFIPRWLFMKRPPLLLPAEGTTGQYDPPGTRLSTSRCWLETDVDDKWEEEEEEEEEEEEEEEEEKAYPSRVPSGGSQGTQCHYSCERQAGKASGGPAPQVPLKGEEVGSDQGLTLSPSILRALEGVQYIADHLRAEDADFSVKEDWKYVAMVIDRIFLWMFIIVCLLGTVGLFLPPYLAGMI E76C6360AF876364 acha4_chick Neuronal acetylcholine receptor subunit alpha-4 CHRNA4 protein protein chick Gallus gallus (Chicken) Chicken MGFLVSKGNLLLLLCASIFPAFGHVETRAHAEERLLKKLFSGYNKWSRPVANISDVVLVRFGLSIAQLIDVDEKNQMMTTNVWVKQEWHDYKLRWDPQEYENVTSIRIPSELIWRPDIVLYNNADGDFAVTHLTKAHLFYDGRIKWMPPAIYKSSCSIDVTFFPFDQQNCKMKFGSWTYDKAKIDLVSMHSHVDQLDYWESGEWVIINAVGNYNSKKYECCTEIYPDITYSFIIRRLPLFYTINLIIPCLLISCLTVLVFYLPSECGEKITLCISVLLSLTVFLLLITEIIPSTSLVIPLIGEYLLFTMIFVTLSIIITVFVLNVHHRSPRTHTMPDWVRRVFLDIVPRLLFMKRPSTVKDNCKKLIESMHKLTNSPRLWSETDMEPNFTTSSSPSPQSNEPSPTSSFCAHLEEPAKPMCKSPSGQYSMLHPEPPQVTCSSPKPSCHPLSDTQTTSISKGRSLSVQQMYSPNKTEEGSIRCRSRSIQYCYLQEDSSQTNGHSSASPASQRCHLNEEQPQHKPHQCKCKCRKGEAAGTPTQGSKSHSNKGEHLVLMSPALKLAVEGVHYIADHLRAEDADFSVKEDWKYVAMVIDRIFLWMFIIVCLLGTVGLFLPPWLAGMI 290B035893D6485A acha5_chick Neuronal acetylcholine receptor subunit alpha-5 CHRNA5 protein protein chick Gallus gallus (Chicken) Chicken MPLRARSRKPGAGPAARAPQAGVSEPSFVAKSEDRLFKHLFEDYQRWVRPVEHLNDTIKIKFGLAISQLVDVDEKNQLMTTNVWLKQEWIHVKLRWNPEDYAGITSIRVPSDSIWIPDIVLYDNADGRFEGTSTKTVVKYDGTIAWTPPVNYKSSCTIDVTFFPFDLQNCSMKFGSWTYDGSQVDIILEDYEVDKRDFFDNGEWEIVTATGSKGNRTDGCCWYPFVTYSFIIRRLPLFYTLFLIIPCIGLSFLTVLVFYLPSNEAEKISLCTSVLVSLTVFLLVIEEIIPSSSKVIPLIGEYLVFTMIFVTLSIVITVFAINIHHRSSSTHNAMAPWVRKIFLHKLPKLLCMRSHVDRYFAQKEEKGNMSGSESSRNTLEAALDSIRYITRHVMKENEVREVVEDWKFIAQVLDRMFLWAFLLVSIIGSLVLFIPVIHKWASIIVPVHIGSTNT A33D79ED1F799776 achb4_chick Neuronal acetylcholine receptor subunit beta-4 CHRNB4 Neuronal acetylcholine receptor non-alpha-3 chain protein protein chick Gallus gallus (Chicken) Chicken STAADAEEKLMNHLLSPDRYNKLIRPAVNSSQLVSIELQVSLAQLISVNEREQIMTTNVWLNQEWIDYRLAWKPSDYEGINMLRIPAKHIWLPDIVLYNNADGTYEVSLYTNAIVQNNGSIRWLPPAIYKSACKIEVKHFPFDQQNCTLKFRSWTYDHTEIDMVLKTSMASMDDFTPSGEWDIVALPGRRTENPLDPNYVDVTYDFIIKRKPLFYTINLIIPCVLITSLAILVFYLPSDCGEKMTLCISVLLALTVFLLLISKIVPPTSLDVPLIGKYLMFTMVLVTFSIVTSVCVLNVHHRSPSTHTMPPWVKLVFLERLPAYLFMKRPENNSPRQKPANCKKTRAENLCMDPADFYKNSTYFVNTASAKKYDMKITDTLDNVSSHQDFRLRTGTKFSPEVQEAIDGVSFIAEHMKSDDNDQSVIEDWKYVAMVVDRLFLWIFVLVCVLGTVGLFLQPLFQNHIAATNP 3117314772886B2D achb2_chick Neuronal acetylcholine receptor subunit beta-2 CHRNB2 Neuronal acetylcholine receptor non-alpha-1 chain protein protein chick Gallus gallus (Chicken) Chicken MALLRVLCLLAALRRSLCTDTEERLVEYLLDPTRYNKLIRPATNGSQLVTVQLMVSLAQLISVHEREQIMTTNVWLTQEWEDYRLTWKPEDFDNMKKVRLPSKHIWLPDVVLYNNADGMYEVSFYSNAVISYDGSIFWLPPAIYKSACKIEVKHFPFDQQNCTMKFRSWTYDRTEIDLVLKSEVASLDDFTPSGEWDIVALPGRRNENPDDSTYVDITYDFIIRRKPLFYTINLIIPCILITSLAILVFYLPSDCGEKMTLCISVLLALTVFLLLISKIVPPTSLDVPLVGKYLMFTMVLVTFSIVTSVCVLNVHHRSPTTHTMPPWVRTLFLRKLPALLFMKQPQQNCARQRLRQRRQTQERAAAATLFLRAGARACACYANPGAAKAEGLNGYRERQGQGPDPPAPCGCGLEEAVEGVRFIADHMRSEDDDQSVSEDWKYVAMVIDRLFLWIFVFVCVFGTVGMFLQPLFQNYATNSLLQLGQGTPTSK 984331E8A76D9750 chrnb2-chrna4-8 1 5 unspecified role unspecified role prey prey 8 unspecified role unspecified role bait bait physical association physical association Fig. 3 (bottom) In Fig 3, the tissue extract was first immunodepleted of alpha3-, beta4- and alpha 5-containing receptors before it was IPed twice with beta2. chrnb2-chrna2-2 1 6 unspecified role unspecified role prey prey 8 unspecified role unspecified role bait bait 4 unspecified role unspecified role prey prey 5 unspecified role unspecified role prey prey association association Fig. 1 (top) chrnb4-chrna3-1 2 7 unspecified role unspecified role bait bait 3 unspecified role unspecified role prey prey physical association physical association Fig. 1 (bottom) chrna2-chrna5-1 1 6 unspecified role unspecified role prey prey 4 unspecified role unspecified role bait bait 8 unspecified role unspecified role prey prey association association Fig. 3 (top) For this IP, the tissue extract was first immunodepleted of alpha3- and beta4-containing receptors before it was IPed twice with alpha2.