IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Rrp8p is a yeast nucleolar protein functionally linked to Gar1p and involved in pre-rRNA cleavage at site A2. Rrp8p is a yeast nucleolar protein functionally linked to Gar1p and involved in pre-rRNA cleavage at site A2. RNA (New York, N.Y.) 2000 imex curation Bousquet-Antonelli C., Vanrobays E., Gélugne JP., Caizergues-Ferrer M., Henry Y. jpg@ibcg+biotoul+fr 17-Sep-2015: Contacted by IntAct-Help. Only protein-protein interactions yeast Saccharomyces cerevisiae Baker's yeast affinity chrom affinity chromatography technology Affinity purification Affinity purification affinity chrom northern blot northern blot northen Bousquet-Antonelli C., Vanrobays E., Gélugne JP., Caizergues-Ferrer M., Henry Y. jpg@ibcg+biotoul+fr Accepted 2015-AUG-10 by ORCHARD RNA (New York, N.Y.) 2000 imex curation Rrp8p is a yeast nucleolar protein functionally linked to Gar1p and involved in pre-rRNA cleavage at site A2. Rrp8p is a yeast nucleolar protein functionally linked to Gar1p and involved in pre-rRNA cleavage at site A2. RNA (New York, N.Y.) 2000 imex curation Bousquet-Antonelli C., Vanrobays E., Gélugne JP., Caizergues-Ferrer M., Henry Y. jpg@ibcg+biotoul+fr 17-Sep-2015: Contacted by IntAct-Help. Only protein-protein interactions yeast Saccharomyces cerevisiae Baker's yeast cosedimentation cosedimentation anti tag western anti tag western blot anti tag western Bousquet-Antonelli C., Vanrobays E., Gélugne JP., Caizergues-Ferrer M., Henry Y. jpg@ibcg+biotoul+fr Accepted 2015-AUG-10 by ORCHARD RNA (New York, N.Y.) 2000 imex curation snr190 yeast small nucleolar RNA 190 yeast snorna small nucleolar rna snoRNA snorna yeast Saccharomyces cerevisiae Baker's yeast GGCCCUGAUGAUAAUGGUGUCUCUUCUUUCCUCGUCCGAUUCGACCAUGACGACAAGGGAUUUUAUCUCGUUCUCUUAAUGCGAAUGAUUUUGAAAAGAUGUUGCUUCUGUGACAUUUUUUUUUAAUCAUUUGUGUUUGCAAACGGGAACUUUUCUUGCCAGUGUUAUACAACACAUGCAGAUCUGAGCC snr42 yeast small nucleolar RNA 42 Yeast snorna small nucleolar rna snoRNA snorna yeast Saccharomyces cerevisiae Baker's yeast AUAACAAUUUGUGAUGCUUUAGGGAGCCUAUUGUUUGAUGGUUUAUAGGAGCCGGAUUUGUCUUUUUGACUAAUUUUCAACCUAUUCUUACUUCCAAAACUGUUGCAAUGGCUCUCGUUUUUUUUAAAUUUUUCUCUUUUCUCUUCUUUGGCAUAGUUUAUUUUUAAUAAAUUAUUUCAGGGUGAGAUAGAGAAAGGAUAAUGAGUAUCGUAGAUAAAUCUUCGGUUCCGAAUAGUGUAACUGUUGGUGCUGAGGUAAUCCAUCUUUAAAACCAUCGCCGUUAGAGGUUGCUUCUGAUAUUUUCGGUUAAGCGACCCAUGAAAUGGUGAUGACCUGAGGUAUGGAACAUCU rl3_yeast 60S ribosomal protein L3 MAK8 RPL3 TCM1 YOR063W YOR29-14 YL1 RP1 Maintenance of killer protein 8 Trichodermin resistance protein Large ribosomal subunit protein uL3 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSHRKYEAPRHGHLGFLPRKRAASIRARVKAFPKDDRSKPVALTSFLGYKAGMTTIVRDLDRPGSKFHKREVVEAVTVVDTPPVVVVGVVGYVETPRGLRSLTTVWAEHLSDEVKRRFYKNWYKSKKKAFTKYSAKYAQDGAGIERELARIKKYASVVRVLVHTQIRKTPLAQKKAHLAEIQLNGGSISEKVDWAREHFEKTVAVDSVFEQNEMIDAIAVTKGHGFEGVTHRWGTKKLPRKTHRGLRKVACIGAWHPAHVMWSVARAGQRGYHSRTSINHKIYRVGKGDDEANGATSFDRTKKTITPMGGFVHYGEIKNDFIMVKGCIPGNRKRIVTLRKSLYTNTSRKALEEVSLKWIDTASKFGKGRFQTPAEKHAFMGTLKKDL 986769A218E9698A fbrl_yeast rRNA 2'-O-methyltransferase fibrillarin NOP1 LOT3 YDL014W D2870 U3 small nucleolar RNA-associated protein NOP1 Histone-glutamine methyltransferase protein protein yeast Saccharomyces cerevisiae Baker's yeast MSFRPGSRGGSRGGSRGGFGGRGGSRGGARGGSRGGFGGRGGSRGGARGGSRGGFGGRGGSRGGARGGSRGGRGGAAGGARGGAKVVIEPHRHAGVYIARGKEDLLVTKNMAPGESVYGEKRISVEEPSKEDGVPPTKVEYRVWNPFRSKLAAGIMGGLDELFIAPGKKVLYLGAASGTSVSHVSDVVGPEGVVYAVEFSHRPGRELISMAKKRPNIIPIIEDARHPQKYRMLIGMVDCVFADVAQPDQARIIALNSHMFLKDQGGVVISIKANCIDSTVDAETVFAREVQKLREERIKPLEQLTLEPYERDHCIVVGRYMRSGLKK 56A8B958A7B6066E 56A8B958A7B6066E gar1_yeast H/ACA ribonucleoprotein complex subunit GAR1 YHR089C GAR1 snoRNP protein GAR1 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSFRGGNRGGRGGFRGGFRGGRTGSARSFQQGPPDTVLEMGAFLHPCEGDIVCRSINTKIPYFNAPIYLENKTQVGKVDEILGPLNEVFFTIKCGDGVQATSFKEGDKFYIAADKLLPIERFLPKPKVVGPPKPKNKKKRSGAPGGRGGASMGRGGSRGGFRGGRGGSSFRGGRGGSSFRGGSRGGSFRGGSRGGSRGGFRGGRR C197FE85644DD0F8 C197FE85644DD0F8 rrp8_yeast 25S rRNA (adenine(645)-N(1))-methyltransferase RRP8 Ribosomal RNA-processing protein 8 D4461 YDR083W protein protein yeast Saccharomyces cerevisiae Baker's yeast MALFNVEGWSIKTKTVAFDNKTNKSSKDKKKNNRKNGKLTREQKLKEETEAELKEQVEDIPSEGSVAKDIPKKNQEKSDQNETSKKRKHDEEAPLMQVKENIEKPTKKQLTPLQQKMMAKLTGSRFRWINEQLYTISSDEALKLIKEQPQLFDEYHDGFRSQVQAWPENPVDVFVDQIRYRCMKPVNAPGGLPGLKDSKEIVIADMGCGEAQLALEINNFFKNYNKKAKKYLKRRHKVHSFDLKKANERITVADIRNVPLPDESCTIVVFCLALMGTNFLDFIKEAYRILAPRGELWIAEIKSRFSDGKGNEFVDALKLMGFFHKKTFDENKMFTRFEFFKPPAEIIEERRQKLERRQKFIEVETEKEELEKKRRKIAEGKWLLKPCIYKRR 58675E3493D9B57B snr10_yeast_rna small nucleolar RNA 10 Yeast snorna small nucleolar rna snoRNA snorna yeast Saccharomyces cerevisiae Baker's yeast AACGCAAAUUUAACAGCCAUUCGUAACACGUACAGUAUCUCGUCGAGGUUGAACCCCUCCGGGGCGUUCUUGACCCAUGAAGAAUGUGGAUUGGUGUUGCAAUAUAUUUAUUAUUGGCUUGGACUGGAGAACAAAUUUAUCGAUCUUGGGUGCAACAGUCUUUCUGUCGUCUGUUUUUUAGCAGAUCUAAGGGUUUACCUUCGUGUGCCCGGAUGAGGACCGUUGCAAGGAUUGAUAAUACAACU rrp8_gar1:rpl3-1 2 5 unspecified role unspecified role neutral component neutral component 7 unspecified role unspecified role neutral component neutral component 8 unspecified role unspecified role neutral component neutral component tag zz tag zz tag ZZ tag ZZ tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range colocalization colocalization Fig9 nop1_snr190-1 1 3 unspecified role unspecified role prey prey 6 unspecified role unspecified role bait bait ZZ tag zz tag zz tag ZZ tag ZZ tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association Fig10B gar1p_snr10_42-1 1 9 unspecified role unspecified role prey prey 4 unspecified role unspecified role prey prey 7 unspecified role unspecified role bait bait zz tag zz tag zz tag ZZ tag ZZ tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range physical association physical association Fig10B