UniProt UniProt http://www.uniprot.org A human HAP1 homologue. Cloning, expression, and interaction with huntingtin. A human HAP1 homologue. Cloning, expression, and interaction with huntingtin. J. Biol. Chem. (0021-9258) 1998 imex curation Li S.H., Hosseini S.H., Gutekunst C.A., Hersch S.M., Ferrante R.J., Li X.J. Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. CoIP experiments using monkey brain tissues omitted as the host organism cannot be determined from the publication. xiaoli@genetics.emory.edu 12-May-2014: Contacted by IntAct-Help. Only protein-protein interactions human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. fluorescence imaging fluorescence microscopy fluorescence imaging immunostaining immunostaining Li S.H., Hosseini S.H., Gutekunst C.A., Hersch S.M., Ferrante R.J., Li X.J. Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. CoIP experiments using monkey brain tissues omitted as the host organism cannot be determined from the publication. xiaoli@genetics.emory.edu Accepted 2014-APR-17 by MGT J. Biol. Chem. (0021-9258) 1998 imex curation A human HAP1 homologue. Cloning, expression, and interaction with huntingtin. A human HAP1 homologue. Cloning, expression, and interaction with huntingtin. J. Biol. Chem. (0021-9258) 1998 imex curation Li S.H., Hosseini S.H., Gutekunst C.A., Hersch S.M., Ferrante R.J., Li X.J. Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. CoIP experiments using monkey brain tissues omitted as the host organism cannot be determined from the publication. xiaoli@genetics.emory.edu 12-May-2014: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro pull down pull down weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Li S.H., Hosseini S.H., Gutekunst C.A., Hersch S.M., Ferrante R.J., Li X.J. Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. CoIP experiments using monkey brain tissues omitted as the host organism cannot be determined from the publication. xiaoli@genetics.emory.edu Accepted 2014-APR-17 by MGT J. Biol. Chem. (0021-9258) 1998 imex curation A human HAP1 homologue. Cloning, expression, and interaction with huntingtin. A human HAP1 homologue. Cloning, expression, and interaction with huntingtin. J. Biol. Chem. (0021-9258) 1998 imex curation Li S.H., Hosseini S.H., Gutekunst C.A., Hersch S.M., Ferrante R.J., Li X.J. Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. CoIP experiments using monkey brain tissues omitted as the host organism cannot be determined from the publication. xiaoli@genetics.emory.edu 12-May-2014: Contacted by IntAct-Help. Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Li S.H., Hosseini S.H., Gutekunst C.A., Hersch S.M., Ferrante R.J., Li X.J. Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. CoIP experiments using monkey brain tissues omitted as the host organism cannot be determined from the publication. xiaoli@genetics.emory.edu Accepted 2014-APR-17 by MGT J. Biol. Chem. (0021-9258) 1998 imex curation A human HAP1 homologue. Cloning, expression, and interaction with huntingtin. A human HAP1 homologue. Cloning, expression, and interaction with huntingtin. J. Biol. Chem. (0021-9258) 1998 imex curation Li S.H., Hosseini S.H., Gutekunst C.A., Hersch S.M., Ferrante R.J., Li X.J. Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. CoIP experiments using monkey brain tissues omitted as the host organism cannot be determined from the publication. xiaoli@genetics.emory.edu 12-May-2014: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Li S.H., Hosseini S.H., Gutekunst C.A., Hersch S.M., Ferrante R.J., Li X.J. Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. CoIP experiments using monkey brain tissues omitted as the host organism cannot be determined from the publication. xiaoli@genetics.emory.edu Accepted 2014-APR-17 by MGT J. Biol. Chem. (0021-9258) 1998 imex curation p54257-2 Huntingtin-associated protein 1 HAP1 HAP2 HLP1 Neuroan 1 protein protein human Homo sapiens Human MRPKRLGRCCAGSRLGPGDPAALTCAPSPSASPAPEPSAQPQARGTGQRVGSRATSGSQFLSEARTGARPASEAGAKAGARRPSAFSAIQGDVRSMPDNSDAPWTRFVFQGPFGSRATGRGTGKAAGIWKTPAAYVGRRPGVSGPERAAFIRELEEALCPNLPPPVKKITQEDVKVMLYLLEELLPPVWESVTYGMVLQRERDLNTAARIGQSLVKQNSVLMEENSKLEALLGSAKEEILYLRHQVNLRDELLQLYSDSDEEDEDEEEEEEEKEAEEEQEEEEAEEDLQCAHPCDAPKLISQEALLHQHHCPQLEALQEKLRLLEEENHQLREEASQLDTLEDEEQMLILECVEQFSEASQQMAELSEVLVLRLENYERQQQEVARLQAQVLKLQQRCRMYGAETEKLQKQLASEKEIQMQLQEEETLPGFQETLAEELRTSLRRMISDPVYFMERNYEMPRGDTSSLRYDFRYSEDREQVRGFEAEEGLMLAADIMRGEDFTPAEEFVPQEELGAAKKVPAEEGVMEEAELVSEETEGWEEVELELDEATRMNVVTSALEASGLGPSHLDMNYVLQQLANWQDAHYRRQLRWKMLQKGECPHGALPAASRTSCRSSCR 6BF49457C5178317 hd_human Huntingtin HTT IT15 HD Huntington disease protein protein protein human Homo sapiens Human MATLEKLMKAFESLKSFQQQQQQQQQQQQQQQQQQQQQPPPPPPPPPPPQLPQPPPQAQPLLPQPQPPPPPPPPPPGPAVAEEPLHRPKKELSATKKDRVNHCLTICENIVAQSVRNSPEFQKLLGIAMELFLLCSDDAESDVRMVADECLNKVIKALMDSNLPRLQLELYKEIKKNGAPRSLRAALWRFAELAHLVRPQKCRPYLVNLLPCLTRTSKRPEESVQETLAAAVPKIMASFGNFANDNEIKVLLKAFIANLKSSSPTIRRTAAGSAVSICQHSRRTQYFYSWLLNVLLGLLVPVEDEHSTLLILGVLLTLRYLVPLLQQQVKDTSLKGSFGVTRKEMEVSPSAEQLVQVYELTLHHTQHQDHNVVTGALELLQQLFRTPPPELLQTLTAVGGIGQLTAAKEESGGRSRSGSIVELIAGGGSSCSPVLSRKQKGKVLLGEEEALEDDSESRSDVSSSALTASVKDEISGELAASSGVSTPGSAGHDIITEQPRSQHTLQADSVDLASCDLTSSATDGDEEDILSHSSSQVSAVPSDPAMDLNDGTQASSPISDSSQTTTEGPDSAVTPSDSSEIVLDGTDNQYLGLQIGQPQDEDEEATGILPDEASEAFRNSSMALQQAHLLKNMSHCRQPSDSSVDKFVLRDEATEPGDQENKPCRIKGDIGQSTDDDSAPLVHCVRLLSASFLLTGGKNVLVPDRDVRVSVKALALSCVGAAVALHPESFFSKLYKVPLDTTEYPEEQYVSDILNYIDHGDPQVRGATAILCGTLICSILSRSRFHVGDWMGTIRTLTGNTFSLADCIPLLRKTLKDESSVTCKLACTAVRNCVMSLCSSSYSELGLQLIIDVLTLRNSSYWLVRTELLETLAEIDFRLVSFLEAKAENLHRGAHHYTGLLKLQERVLNNVVIHLLGDEDPRVRHVAAASLIRLVPKLFYKCDQGQADPVVAVARDQSSVYLKLLMHETQPPSHFSVSTITRIYRGYNLLPSITDVTMENNLSRVIAAVSHELITSTTRALTFGCCEALCLLSTAFPVCIWSLGWHCGVPPLSASDESRKSCTVGMATMILTLLSSAWFPLDLSAHQDALILAGNLLAASAPKSLRSSWASEEEANPAATKQEEVWPALGDRALVPMVEQLFSHLLKVINICAHVLDDVAPGPAIKAALPSLTNPPSLSPIRRKGKEKEPGEQASVPLSPKKGSEASAASRQSDTSGPVTTSKSSSLGSFYHLPSYLKLHDVLKATHANYKVTLDLQNSTEKFGGFLRSALDVLSQILELATLQDIGKCVEEILGYLKSCFSREPMMATVCVQQLLKTLFGTNLASQFDGLSSNPSKSQGRAQRLGSSSVRPGLYHYCFMAPYTHFTQALADASLRNMVQAEQENDTSGWFDVLQKVSTQLKTNLTSVTKNRADKNAIHNHIRLFEPLVIKALKQYTTTTCVQLQKQVLDLLAQLVQLRVNYCLLDSDQVFIGFVLKQFEYIEVGQFRESEAIIPNIFFFLVLLSYERYHSKQIIGIPKIIQLCDGIMASGRKAVTHAIPALQPIVHDLFVLRGTNKADAGKELETQKEVVVSMLLRLIQYHQVLEMFILVLQQCHKENEDKWKRLSRQIADIILPMLAKQQMHIDSHEALGVLNTLFEILAPSSLRPVDMLLRSMFVTPNTMASVSTVQLWISGILAILRVLISQSTEDIVLSRIQELSFSPYLISCTVINRLRDGDSTSTLEEHSEGKQIKNLPEETFSRFLLQLVGILLEDIVTKQLKVEMSEQQHTFYCQELGTLLMCLIHIFKSGMFRRITAAATRLFRSDGCGGSFYTLDSLNLRARSMITTHPALVLLWCQILLLVNHTDYRWWAEVQQTPKRHSLSSTKLLSPQMSGEEEDSDLAAKLGMCNREIVRRGALILFCDYVCQNLHDSEHLTWLIVNHIQDLISLSHEPPVQDFISAVHRNSAASGLFIQAIQSRCENLSTPTMLKKTLQCLEGIHLSQSGAVLTLYVDRLLCTPFRVLARMVDILACRRVEMLLAANLQSSMAQLPMEELNRIQEYLQSSGLAQRHQRLYSLLDRFRLSTMQDSLSPSPPVSSHPLDGDGHVSLETVSPDKDWYVHLVKSQCWTRSDSALLEGAELVNRIPAEDMNAFMMNSEFNLSLLAPCLSLGMSEISGGQKSALFEAAREVTLARVSGTVQQLPAVHHVFQPELPAEPAAYWSKLNDLFGDAALYQSLPTLARALAQYLVVVSKLPSHLHLPPEKEKDIVKFVVATLEALSWHLIHEQIPLSLDLQAGLDCCCLALQLPGLWSVVSSTEFVTHACSLIYCVHFILEAVAVQPGEQLLSPERRTNTPKAISEEEEEVDPNTQNPKYITAACEMVAEMVESLQSVLALGHKRNSGVPAFLTPLLRNIIISLARLPLVNSYTRVPPLVWKLGWSPKPGGDFGTAFPEIPVEFLQEKEVFKEFIYRINTLGWTSRTQFEETWATLLGVLVTQPLVMEQEESPPEEDTERTQINVLAVQAITSLVLSAMTVPVAGNPAVSCLEQQPRNKPLKALDTRFGRKLSIIRGIVEQEIQAMVSKRENIATHHLYQAWDPVPSLSPATTGALISHEKLLLQINPERELGSMSYKLGQVSIHSVWLGNSITPLREEEWDEEEEEEADAPAPSSPPTSPVNSRKHRAGVDIHSCSQFLLELYSRWILPSSSARRTPAILISEVVRSLLVVSDLFTERNQFELMYVTLTELRRVHPSEDEILAQYLVPATCKAAAVLGMDKAVAEPVSRLLESTLRSSHLPSRVGALHGVLYVLECDLLDDTAKQLIPVISDYLLSNLKGIAHCVNIHSQQHVLVMCATAFYLIENYPLDVGPEFSASIIQMCGVMLSGSEESTPSIIYHCALRGLERLLLSEQLSRLDAESLVKLSVDRVNVHSPHRAMAALGLMLTCMYTGKEKVSPGRTSDPNPAAPDSESVIVAMERVSVLFDRIRKGFPCEARVVARILPQFLDDFFPPQDIMNKVIGEFLSNQQPYPQFMATVVYKVFQTLHSTGQSSMVRDWVMLSLSNFTQRAPVAMATWSLSCFFVSASTSPWVAAILPHVISRMGKLEQVDVNLFCLVATDFYRHQIEEELDRRAFQSVLEVVAAPGSPYHRLLTCLRNVHKVTTC DIP protein P42858 original sequence version: 148 DIP protein P42858 original sequence version: 145 DIP protein P42858 original sequence version: 142 DIP protein P42858 original sequence version: 157 DIP protein P42858 original sequence version: 158 DIP protein P42858 original sequence version: 156 A267509E84D52F0D p54256-2 Huntingtin-associated protein 1 Short Hap1 protein protein rat Rattus norvegicus (Rat) Rat MRPKDQVQSSAGDGTGSGDPATGTPTTQPAADPAPEPSAEPKPAPAQGTGSGQKSGSRTKTGGSFCRSRIRGDSDAPWTRYIFQGPYGPRATGLGTGRAEGIWKTPAAYIGRRPGVSGPERAAFIRELQEALCPNPLPRKKITEDDIKVMLYLLEEKERDLNTAARIGQSLVKQNSVLMEENNKLETMLGSAREEILHLRKQVNLRDDLLQLYSDSDDDEEDEEDEEEEEGEEEEREGQRDQDQQHDHPYGAPKPPPKAETLHHCPQLEALKQKLKLLEEENDHLREEASHLDNLEDKEQMLILECVEQFSEASQQMAELSEVLVLRLEGYERQQKEITQLQAEITKLQQRCQSYGAQTEKLQQQLASEKGVHPESLRAGSHMQDYGSRPRERQEDGKSHRQRSSMPAGSVTHYGYSVPLDALPSFPETLAEELRTSLRKFITDPAYFMERCDTRCREERKKEQGTMPPPPVQDLKPPEDFEAPEELVPEEELGAIEEVGTAEDGPAEETEQASEETEAWEEVEPEVDEATRMNVVVSALEASGLGPSHLDMKYVLQQLSNWQDAHSKRQQKQKVVPKGECSRRGHPPASGTSYRSSTL Contains a phosphothreonine at position 598. DA2949D7676C9434 hap1-htt-2 2 5 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain n_terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) 6 unspecified role unspecified role prey prey in vitro translated in vitro translated protein in vitro translated P42858:p.Gln18[44] q44 poly-glutamine extension mutation increasing mutation increasing interaction mutation increasing range range range range QQQQQQQQQQQQQQQQQQQQQ QQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQ Wed Jun 13 19:03:31 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'p.gln18[44]' Tue Jun 30 16:33:29 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln18[44]' region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain range range P42858:p.Gln18[23] q23 poly-glutamine extension mutation mutation range range range range QQQQQQQQQQQQQQQQQQQQQ QQQQQQQQQQQQQQQQQQQQQQQ Wed Jun 13 18:25:45 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'p.gln18[23]' Tue Jun 30 18:32:38 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln18[23]' region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined P42858:p.Gln18[73] q73 poly-glutamine extension mutation increasing mutation increasing interaction mutation increasing range range range range QQQQQQQQQQQQQQQQQQQQQ QQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQ Wed Jun 13 18:35:52 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'p.gln18[73]' Tue Jun 30 17:29:09 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln18[73]' The poly-Gln region of HTT is highly polymorphic. A variant with 23Q (UniProt FTId: VAR_005268) has been used as 'wild-type'. A HTT constructs with 44Q or 73Q show increased interaction. physical association physical association 5c htt-hap1-4 3 6 unspecified role unspecified role bait bait P42858:p.Gln18[23] q23 poly-glutamine extension mutation mutation range range range range QQQQQQQQQQQQQQQQQQQQQ QQQQQQQQQQQQQQQQQQQQQQQ Wed Jun 13 17:40:24 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'p.gln18[23]' Tue Jun 30 15:53:08 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln18[23]' region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain range range P42858:p.Gln18[44] q44 poly-glutamine extension mutation increasing mutation increasing interaction mutation increasing range range range range QQQQQQQQQQQQQQQQQQQQQ QQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQ Wed Jun 13 18:54:55 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'p.gln18[44]' Tue Jun 30 19:46:34 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln18[44]' The poly-Gln region of HTT is highly polymorphic. A variant with 23Q (UniProt FTId: VAR_005268) has been used as 'wild-type'. A HTT construct with 44Q shows increased interaction. 5 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association 5b htt-hap1-4 3 7 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 6 unspecified role unspecified role bait bait P42858:p.Gln18[44] q44 poly-glutamine extension mutation increasing mutation increasing interaction mutation increasing range range range range QQQQQQQQQQQQQQQQQQQQQ QQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQ Wed Jun 13 18:46:54 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'p.gln18[44]' Tue Jun 30 19:22:04 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln18[44]' region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain range range P42858:p.Gln18[23] q23 poly-glutamine extension mutation mutation range range range range QQQQQQQQQQQQQQQQQQQQQ QQQQQQQQQQQQQQQQQQQQQQQ Wed Jun 13 18:35:01 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'p.gln18[23]' Tue Jun 30 16:07:51 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln18[23]' The poly-Gln region of HTT is highly polymorphic. A variant with 23Q (UniProt FTId: VAR_005268) has been used as 'wild-type'. A HTT construct with 44Q shows increased interaction. physical association physical association 5b hap1-htt-1 4 6 unspecified role unspecified role prey prey cell lysate cell lysate human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. The poly-Gln region of HTT is highly polymorphic. A variant with 23Q (UniProt FTId: VAR_005268) has been used as 'wild-type'. A HTT constructs with 44Q or 73Q show increased interaction. 5 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain n_terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) physical association physical association 6a hap1-htt-3 1 5 unspecified role unspecified role neutral component neutral component 6 unspecified role unspecified role neutral component neutral component colocalization colocalization 7ef Huntingtin was co-localized with hHAP on the perinuclear inclusions.