IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Immunochemical characterization and transacting properties of upstream stimulatory factor isoforms. Immunochemical characterization and transacting properties of upstream stimulatory factor isoforms. J. Biol. Chem. (0021-9258) 1996 imex curation Viollet B., Lefrançois-Martinez A.M., Henrion A., Kahn A., Raymondjean M., Martinez A. 11-Jun-2014: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro emsa supershift electrophoretic mobility supershift assay emsa supershift tag visualisation tag visualisation Viollet B., Lefrançois-Martinez A.M., Henrion A., Kahn A., Raymondjean M., Martinez A. Only the in vitro experiments were curated as in vivo the bands could potentially come from any protein binding to the MLP probe. Fig. 5b, lane 12, has not been curated as the antibody recognises both isoforms, USF2a and USF2b, but the band cannot distinguish which homo- or heterodimer were in fact supershifted. Accepted 2014-MAY-23 by ORCHARD J. Biol. Chem. (0021-9258) 1996 Only protein-protein interactions imex curation Immunochemical characterization and transacting properties of upstream stimulatory factor isoforms. Immunochemical characterization and transacting properties of upstream stimulatory factor isoforms. J. Biol. Chem. (0021-9258) 1996 imex curation Viollet B., Lefrançois-Martinez A.M., Henrion A., Kahn A., Raymondjean M., Martinez A. 11-Jun-2014: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro emsa electrophoretic mobility shift assay Gel retardation assay band shift band shift Gel retardation assay emsa tag visualisation tag visualisation Viollet B., Lefrançois-Martinez A.M., Henrion A., Kahn A., Raymondjean M., Martinez A. Only the in vitro experiments were curated as in vivo the bands could potentially come from any protein binding to the MLP probe. Accepted 2014-MAY-23 by ORCHARD J. Biol. Chem. (0021-9258) 1996 Only protein-protein interactions imex curation q15853-3 Upstream stimulatory factor 2 USF2 BHLHB12 Class B basic helix-loop-helix protein 12 FOS-interacting protein Major late transcription factor 2 Upstream transcription factor 2 protein protein human Homo sapiens Human MDMLDPGLDPAASATAAAAASHDKGPEAEEGVELQEGGDGPGAEEQTAVAITSVQQAAFGDHNIQYQFRTETNGGQAVIQNPFSNGGSPAAEAVSGEARFAYFPASSVGDTTAVSVQTTDQSLQAGGQFYVMMTPQDVLQTGTQRTIAPRTHPYSPKIDGTRTPRDERRRAQHNEVERRRRDKINNWIVQLSKIIPDCNADNSKTGASKGGILSKACDYIRELRQTNQRMQETFKEAERLQMDNELLRQQIEELKNENALLRAQLQQHNLEMVGEGTRQ 15C53A2CC946CEA7 mlp-dsdna probe MLP-dsDNA probe ds dna double stranded deoxyribonucleic acid ds DNA ds dna chemical synthesis Chemical synthesis (Chemical synthesis) GTAGGCCACGTGACCGGGT usf2_human Upstream stimulatory factor 2 USF2 Upstream transcription factor 2 FOS-interacting protein Major late transcription factor 2 BHLHB12 Class B basic helix-loop-helix protein 12 protein protein human Homo sapiens Human MDMLDPGLDPAASATAAAAASHDKGPEAEEGVELQEGGDGPGAEEQTAVAITSVQQAAFGDHNIQYQFRTETNGGQVTYRVVQVTDGQLDGQGDTAGAVSVVSTAAFAGGQQAVTQVGVDGAAQRPGPAAASVPPGPAAPFPLAVIQNPFSNGGSPAAEAVSGEARFAYFPASSVGDTTAVSVQTTDQSLQAGGQFYVMMTPQDVLQTGTQRTIAPRTHPYSPKIDGTRTPRDERRRAQHNEVERRRRDKINNWIVQLSKIIPDCNADNSKTGASKGGILSKACDYIRELRQTNQRMQETFKEAERLQMDNELLRQQIEELKNENALLRAQLQQHNLEMVGEGTRQ 78CEFE97AC4C10CF q15853-1 Upstream stimulatory factor 2 USF2 BHLHB12 Class B basic helix-loop-helix protein 12 FOS-interacting protein Major late transcription factor 2 Upstream transcription factor 2 protein protein human Homo sapiens Human MDMLDPGLDPAASATAAAAASHDKGPEAEEGVELQEGGDGPGAEEQTAVAITSVQQAAFGDHNIQYQFRTETNGGQVTYRVVQVTDGQLDGQGDTAGAVSVVSTAAFAGGQQAVTQVGVDGAAQRPGPAAASVPPGPAAPFPLAVIQNPFSNGGSPAAEAVSGEARFAYFPASSVGDTTAVSVQTTDQSLQAGGQFYVMMTPQDVLQTGTQRTIAPRTHPYSPKIDGTRTPRDERRRAQHNEVERRRRDKINNWIVQLSKIIPDCNADNSKTGASKGGILSKACDYIRELRQTNQRMQETFKEAERLQMDNELLRQQIEELKNENALLRAQLQQHNLEMVGEGTRQ 78CEFE97AC4C10CF usf1_human Upstream stimulatory factor 1 Major late transcription factor 1 USF1 USF BHLHB11 Class B basic helix-loop-helix protein 11 protein protein human Homo sapiens Human MKGQQKTAETEEGTVQIQEGAVATGEDPTSVAIASIQSAATFPDPNVKYVFRTENGGQVMYRVIQVSEGQLDGQTEGTGAISGYPATQSMTQAVIQGAFTSDDAVDTEGTAAETHYTYFPSTAVGDGAGGTTSGSTAAVVTTQGSEALLGQATPPGTGQFFVMMSPQEVLQGGSQRSIAPRTHPYSPKSEAPRTTRDEKRRAQHNEVERRRRDKINNWIVQLSKIIPDCSMESTKSGQSKGGILSKACDYIQELRQSNHRLSEELQGLDQLQLDNDVLRQQVEDLKNKNLLLRAQLRHHGLEVVIKNDSN BFDA91519B4B80AE mlp-usf1-1 2 4 unspecified role unspecified role bait bait region 32p radiolabel 32p radiolabel P32 32P 32P P32 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined chemical synthesis Chemical synthesis (Chemical synthesis) 7 weight identificat confirmation by molecular weight weight identificat tag visualisation tag visualisation unspecified role unspecified role prey prey myc-type, basic helix-loop-helix (bhlh) domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain rabit-reticulocyte Oryctolagus cuniculus reticulocyte reticulocyte Reticulocytes physical association physical association Fig. 2b, lanes 3, 8, 9 & 10; Fig. 5b, lanes 1, 4, 5, 7 & 8 mlp-usf1-usf2b-1 2 3 weight identificat confirmation by molecular weight weight identificat tag visualisation tag visualisation unspecified role unspecified role prey prey rabit-reticulocyte Oryctolagus cuniculus reticulocyte reticulocyte Reticulocytes 7 weight identificat confirmation by molecular weight weight identificat tag visualisation tag visualisation unspecified role unspecified role prey prey rabit-reticulocyte Oryctolagus cuniculus reticulocyte reticulocyte Reticulocytes 4 unspecified role unspecified role bait bait region 32p radiolabel 32p radiolabel P32 32P 32P P32 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined chemical synthesis Chemical synthesis (Chemical synthesis) physical association physical association Fig. 2b, lane 3; Fig. 5b, lane 7 mlp-usf2b-1 2 3 weight identificat confirmation by molecular weight weight identificat tag visualisation tag visualisation unspecified role unspecified role prey prey rabit-reticulocyte Oryctolagus cuniculus reticulocyte reticulocyte Reticulocytes 4 unspecified role unspecified role bait bait region 32p radiolabel 32p radiolabel P32 32P 32P P32 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined chemical synthesis Chemical synthesis (Chemical synthesis) physical association physical association Fig. 2b, lanes 2 & 3; Fig. 5b, lanes 3, 7, 9, 10 & 11 mlp-usf2a-usf2b-1 2 4 unspecified role unspecified role bait bait region 32p radiolabel 32p radiolabel P32 32P 32P P32 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined chemical synthesis Chemical synthesis (Chemical synthesis) 3 weight identificat confirmation by molecular weight weight identificat tag visualisation tag visualisation unspecified role unspecified role prey prey rabit-reticulocyte Oryctolagus cuniculus reticulocyte reticulocyte Reticulocytes 6 weight identificat confirmation by molecular weight weight identificat tag visualisation tag visualisation unspecified role unspecified role prey prey rabit-reticulocyte Oryctolagus cuniculus reticulocyte reticulocyte Reticulocytes physical association physical association Fig. 2b, lane 2; Fig. 5b, lane 10 mlp-usf2a-1 2 6 weight identificat confirmation by molecular weight weight identificat tag visualisation tag visualisation unspecified role unspecified role prey prey rabit-reticulocyte Oryctolagus cuniculus reticulocyte reticulocyte Reticulocytes 4 unspecified role unspecified role bait bait region 32p radiolabel 32p radiolabel P32 32P 32P P32 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined chemical synthesis Chemical synthesis (Chemical synthesis) physical association physical association Fig. 2b, lanes 2, 9 & 11; Fig. 5b, lanes 2, 4, 6 & 10 mlp-usf1-2 2 4 unspecified role unspecified role bait bait region 32p radiolabel 32p radiolabel P32 32P 32P P32 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined chemical synthesis Chemical synthesis (Chemical synthesis) 7 weight identificat confirmation by molecular weight weight identificat tag visualisation tag visualisation unspecified role unspecified role prey prey ecoli Escherichia coli (strain K12) physical association physical association Fig. 5a, lanes 7, 9 & 10 mlp-usf2a-2 2 6 weight identificat confirmation by molecular weight weight identificat tag visualisation tag visualisation unspecified role unspecified role prey prey ecoli Escherichia coli (strain K12) 4 unspecified role unspecified role bait bait region 32p radiolabel 32p radiolabel P32 32P 32P P32 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined chemical synthesis Chemical synthesis (Chemical synthesis) physical association physical association Fig. 5a, lanes 1, 2, 6, 8 & 9 mlp-usf1-usf2a-1 2 4 unspecified role unspecified role bait bait region 32p radiolabel 32p radiolabel P32 32P 32P P32 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined chemical synthesis Chemical synthesis (Chemical synthesis) 6 weight identificat confirmation by molecular weight weight identificat tag visualisation tag visualisation unspecified role unspecified role prey prey rabit-reticulocyte Oryctolagus cuniculus reticulocyte reticulocyte Reticulocytes 7 weight identificat confirmation by molecular weight weight identificat tag visualisation tag visualisation unspecified role unspecified role prey prey myc-type, basic helix-loop-helix (bhlh) domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain rabit-reticulocyte Oryctolagus cuniculus reticulocyte reticulocyte Reticulocytes physical association physical association Fig. 2b, lane 9; Fig. 5b, lane 4 mlp-usf1-usf2-1 2 4 unspecified role unspecified role bait bait region 32p radiolabel 32p radiolabel P32 32P 32P P32 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined chemical synthesis Chemical synthesis (Chemical synthesis) 5 weight identificat confirmation by molecular weight weight identificat tag visualisation tag visualisation unspecified role unspecified role prey prey myc-type, basic helix-loop-helix (bhlh) domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain rabit-reticulocyte Oryctolagus cuniculus reticulocyte reticulocyte Reticulocytes 7 weight identificat confirmation by molecular weight weight identificat tag visualisation tag visualisation unspecified role unspecified role prey prey rabit-reticulocyte Oryctolagus cuniculus reticulocyte reticulocyte Reticulocytes physical association physical association Fig. 2b, lane 10 mlp-usf2-1 2 5 weight identificat confirmation by molecular weight weight identificat tag visualisation tag visualisation unspecified role unspecified role prey prey myc-type, basic helix-loop-helix (bhlh) domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain rabit-reticulocyte Oryctolagus cuniculus reticulocyte reticulocyte Reticulocytes 4 unspecified role unspecified role bait bait region 32p radiolabel 32p radiolabel P32 32P 32P P32 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined chemical synthesis Chemical synthesis (Chemical synthesis) physical association physical association Fig. 2b, lanes 10 & 11 mlp-usf2b-2 2 3 weight identificat confirmation by molecular weight weight identificat tag visualisation tag visualisation unspecified role unspecified role prey prey ecoli Escherichia coli (strain K12) 4 unspecified role unspecified role bait bait region 32p radiolabel 32p radiolabel P32 32P 32P P32 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined chemical synthesis Chemical synthesis (Chemical synthesis) physical association physical association Fig. 5a, lanes 2-5 mlp-usf2a-usf2b-2 2 6 weight identificat confirmation by molecular weight weight identificat tag visualisation tag visualisation unspecified role unspecified role prey prey ecoli Escherichia coli (strain K12) 4 unspecified role unspecified role bait bait region 32p radiolabel 32p radiolabel P32 32P 32P P32 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined chemical synthesis Chemical synthesis (Chemical synthesis) 3 weight identificat confirmation by molecular weight weight identificat tag visualisation tag visualisation unspecified role unspecified role prey prey ecoli Escherichia coli (strain K12) physical association physical association Fig. 5a, lanes 1, 2 & 4 mlp-usf1-usf2a-2 2 7 weight identificat confirmation by molecular weight weight identificat tag visualisation tag visualisation unspecified role unspecified role prey prey ecoli Escherichia coli (strain K12) 6 weight identificat confirmation by molecular weight weight identificat tag visualisation tag visualisation unspecified role unspecified role prey prey ecoli Escherichia coli (strain K12) 4 unspecified role unspecified role bait bait region 32p radiolabel 32p radiolabel P32 32P 32P P32 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined chemical synthesis Chemical synthesis (Chemical synthesis) physical association physical association Fig. 5a, lanes 8-10 mlp-usf2a-3 1 4 unspecified role unspecified role bait bait region 32p radiolabel 32p radiolabel P32 32P 32P P32 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined chemical synthesis Chemical synthesis (Chemical synthesis) 6 weight identificat confirmation by molecular weight weight identificat tag visualisation tag visualisation unspecified role unspecified role prey prey rabit-reticulocyte Oryctolagus cuniculus reticulocyte reticulocyte Reticulocytes physical association physical association Fig. 5b, lanes 5 & 11 mlp-usf1-3 1 4 unspecified role unspecified role bait bait region 32p radiolabel 32p radiolabel P32 32P 32P P32 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined chemical synthesis Chemical synthesis (Chemical synthesis) 7 weight identificat confirmation by molecular weight weight identificat tag visualisation tag visualisation unspecified role unspecified role prey prey rabit-reticulocyte Oryctolagus cuniculus reticulocyte reticulocyte Reticulocytes physical association physical association Fig. 5b, lanes 6 & 9 mlp-usf2b-3 1 3 weight identificat confirmation by molecular weight weight identificat tag visualisation tag visualisation unspecified role unspecified role prey prey rabit-reticulocyte Oryctolagus cuniculus reticulocyte reticulocyte Reticulocytes 4 unspecified role unspecified role bait bait region 32p radiolabel 32p radiolabel P32 32P 32P P32 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined chemical synthesis Chemical synthesis (Chemical synthesis) physical association physical association Fig. 5b, lane 8