IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Mechanism of platelet-derived growth factor (PDGF) AA, AB, and BB binding to alpha and beta PDGF receptor. Mechanism of platelet-derived growth factor (PDGF) AA, AB, and BB binding to alpha and beta PDGF receptor. J. Biol. Chem. (0021-9258) 1993 imex curation Fretto L.J., Snape A.J., Tomlinson J.E., Seroogy J.J., Wolf D.L., LaRochelle W.J., Giese N.A. 18-Feb-2014: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro competition binding competition binding tag visualisation tag visualisation Fretto L.J., Snape A.J., Tomlinson J.E., Seroogy J.J., Wolf D.L., LaRochelle W.J., Giese N.A. From text - showing that they did incubate with both radiolabelled AND free PDGF together -(despite expt details being very unclear):- "When PDGF receptor extracellular domain was immobilized in microtiter wells (see "Experimental Procedures") and incubated with increasing concentrations of "'1-PDGF AA, AB, or BB in the presence or absence of excess unlabeled ligand, specific binding was saturable and was >80% of total binding for each of the ligand concentrations tested....." You have not added Fig 8 without the competitor. This is a Scachard Analysis in which the ratio of bound/free ligand is plotted against increasing concentrations of bound ligand. Accepted 2014-FEB-11 by MGT J. Biol. Chem. (0021-9258) 1993 Only protein-protein interactions imex curation Mechanism of platelet-derived growth factor (PDGF) AA, AB, and BB binding to alpha and beta PDGF receptor. Mechanism of platelet-derived growth factor (PDGF) AA, AB, and BB binding to alpha and beta PDGF receptor. J. Biol. Chem. (0021-9258) 1993 imex curation Fretto L.J., Snape A.J., Tomlinson J.E., Seroogy J.J., Wolf D.L., LaRochelle W.J., Giese N.A. 18-Feb-2014: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro elisa enzyme linked immunosorbent assay ELISA ELISA elisa antibody detection identification by antibody antibody detection Fretto L.J., Snape A.J., Tomlinson J.E., Seroogy J.J., Wolf D.L., LaRochelle W.J., Giese N.A. Accepted 2014-FEB-11 by MGT J. Biol. Chem. (0021-9258) 1993 Only protein-protein interactions imex curation Mechanism of platelet-derived growth factor (PDGF) AA, AB, and BB binding to alpha and beta PDGF receptor. Mechanism of platelet-derived growth factor (PDGF) AA, AB, and BB binding to alpha and beta PDGF receptor. J. Biol. Chem. (0021-9258) 1993 imex curation Fretto L.J., Snape A.J., Tomlinson J.E., Seroogy J.J., Wolf D.L., LaRochelle W.J., Giese N.A. 18-Feb-2014: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro electrophoresis electrophoretic mobility-based method electrophoresis predetermined predetermined participant predetermined Fretto L.J., Snape A.J., Tomlinson J.E., Seroogy J.J., Wolf D.L., LaRochelle W.J., Giese N.A. Accepted 2014-FEB-11 by MGT J. Biol. Chem. (0021-9258) 1993 Only protein-protein interactions imex curation Mechanism of platelet-derived growth factor (PDGF) AA, AB, and BB binding to alpha and beta PDGF receptor. Mechanism of platelet-derived growth factor (PDGF) AA, AB, and BB binding to alpha and beta PDGF receptor. J. Biol. Chem. (0021-9258) 1993 imex curation Fretto L.J., Snape A.J., Tomlinson J.E., Seroogy J.J., Wolf D.L., LaRochelle W.J., Giese N.A. 18-Feb-2014: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro comig non denat gel comigration in non denaturing gel electrophoresis comig non denat gel weight silver stain molecular weight estimation by silver staining weight silver stain Fretto L.J., Snape A.J., Tomlinson J.E., Seroogy J.J., Wolf D.L., LaRochelle W.J., Giese N.A. Accepted 2014-FEB-11 by MGT J. Biol. Chem. (0021-9258) 1993 Only protein-protein interactions imex curation pgfra_human Platelet-derived growth factor receptor alpha PDGFRA CD140 antigen-like family member A CD140a antigen Alpha-type platelet-derived growth factor receptor Alpha platelet-derived growth factor receptor Platelet-derived growth factor receptor 2 RHEPDGFRA PDGFR2 Platelet-derived growth factor alpha receptor protein protein human Homo sapiens Human MGTSHPAFLVLGCLLTGLSLILCQLSLPSILPNENEKVVQLNSSFSLRCFGESEVSWQYPMSEEESSDVEIRNEENNSGLFVTVLEVSSASAAHTGLYTCYYNHTQTEENELEGRHIYIYVPDPDVAFVPLGMTDYLVIVEDDDSAIIPCRTTDPETPVTLHNSEGVVPASYDSRQGFNGTFTVGPYICEATVKGKKFQTIPFNVYALKATSELDLEMEALKTVYKSGETIVVTCAVFNNEVVDLQWTYPGEVKGKGITMLEEIKVPSIKLVYTLTVPEATVKDSGDYECAARQATREVKEMKKVTISVHEKGFIEIKPTFSQLEAVNLHEVKHFVVEVRAYPPPRISWLKNNLTLIENLTEITTDVEKIQEIRYRSKLKLIRAKEEDSGHYTIVAQNEDAVKSYTFELLTQVPSSILDLVDDHHGSTGGQTVRCTAEGTPLPDIEWMICKDIKKCNNETSWTILANNVSNIITEIHSRDRSTVEGRVTFAKVEETIAVRCLAKNLLGAENRELKLVAPTLRSELTVAAAVLVLLVIVIISLIVLVVIWKQKPRYEIRWRVIESISPDGHEYIYVDPMQLPYDSRWEFPRDGLVLGRVLGSGAFGKVVEGTAYGLSRSQPVMKVAVKMLKPTARSSEKQALMSELKIMTHLGPHLNIVNLLGACTKSGPIYIITEYCFYGDLVNYLHKNRDSFLSHHPEKPKKELDIFGLNPADESTRSYVILSFENNGDYMDMKQADTTQYVPMLERKEVSKYSDIQRSLYDRPASYKKKSMLDSEVKNLLSDDNSEGLTLLDLLSFTYQVARGMEFLASKNCVHRDLAARNVLLAQGKIVKICDFGLARDIMHDSNYVSKGSTFLPVKWMAPESIFDNLYTTLSDVWSYGILLWEIFSLGGTPYPGMMVDSTFYNKIKSGYRMAKPDHATSEVYEIMVKCWNSEPEKRPSFYHLSEIVENLLPGQYKKSYEKIHLDFLKSDHPAVARMRVDSDNAYIGVTYKNEEDKLKDWEGGLDEQRLSADSGYIIPLPDIDPVPEEEDLGKRNRHSSQTSEESAIETGSSSSTFIKREDETIEDIDMMDDIGIDSSDLVEDSFL 5E3FB9940ACD1BE8 pdgfa_human Platelet-derived growth factor subunit A PDGFA PDGF1 Platelet-derived growth factor A chain Platelet-derived growth factor alpha polypeptide PDGF-1 protein protein human Homo sapiens Human MRTLACLLLLGCGYLAHVLAEEAEIPREVIERLARSQIHSIRDLQRLLEIDSVGSEDSLDTSLRAHGVHATKHVPEKRPLPIRRKRSIEEAVPAVCKTRTVIYEIPRSQVDPTSANFLIWPPCVEVKRCTGCCNTSSVKCQPSRVHHRSVKVAKVEYVRKKPKLKEVQVRLEEHLECACATTSLNPDYREEDTGRPRESGKKRKRKRLKPT 48633DDE558EFA43 pdgfb_human Platelet-derived growth factor subunit B Platelet-derived growth factor B chain Platelet-derived growth factor beta polypeptide PDGF-2 Proto-oncogene c-Sis PDGFB PDGF2 SIS protein protein human Homo sapiens Human MNRCWALFLSLCCYLRLVSAEGDPIPEELYEMLSDHSIRSFDDLQRLLHGDPGEEDGAELDLNMTRSHSGGELESLARGRRSLGSLTIAEPAMIAECKTRTEVFEISRRLIDRTNANFLVWPPCVEVQRCSGCCNNRNVQCRPTQVQLRPVQVRKIEIVRKKPIFKKATVTLEDHLACKCETVAAARPVTRSPGGSQEQRAKTPQTRVTIRTVRVRRPPKGKHRKFKHTHDKTALKETLGA DIP protein P01127-1 original sequence version: 170 DIP protein P01127 original sequence version: 145 DIP protein P01127 original sequence version: 162 9F9A3474CE203C0B pgfrb_human Platelet-derived growth factor receptor beta CD140 antigen-like family member B PDGFRB Beta-type platelet-derived growth factor receptor PDGFR Platelet-derived growth factor receptor 1 Beta platelet-derived growth factor receptor PDGFR1 protein protein human Homo sapiens Human MRLPGAMPALALKGELLLLSLLLLLEPQISQGLVVTPPGPELVLNVSSTFVLTCSGSAPVVWERMSQEPPQEMAKAQDGTFSSVLTLTNLTGLDTGEYFCTHNDSRGLETDERKRLYIFVPDPTVGFLPNDAEELFIFLTEITEITIPCRVTDPQLVVTLHEKKGDVALPVPYDHQRGFSGIFEDRSYICKTTIGDREVDSDAYYVYRLQVSSINVSVNAVQTVVRQGENITLMCIVIGNEVVNFEWTYPRKESGRLVEPVTDFLLDMPYHIRSILHIPSAELEDSGTYTCNVTESVNDHQDEKAINITVVESGYVRLLGEVGTLQFAELHRSRTLQVVFEAYPPPTVLWFKDNRTLGDSSAGEIALSTRNVSETRYVSELTLVRVKVAEAGHYTMRAFHEDAEVQLSFQLQINVPVRVLELSESHPDSGEQTVRCRGRGMPQPNIIWSACRDLKRCPRELPPTLLGNSSEEESQLETNVTYWEEEQEFEVVSTLRLQHVDRPLSVRCTLRNAVGQDTQEVIVVPHSLPFKVVVISAILALVVLTIISLIILIMLWQKKPRYEIRWKVIESVSSDGHEYIYVDPMQLPYDSTWELPRDQLVLGRTLGSGAFGQVVEATAHGLSHSQATMKVAVKMLKSTARSSEKQALMSELKIMSHLGPHLNVVNLLGACTKGGPIYIITEYCRYGDLVDYLHRNKHTFLQHHSDKRRPPSAELYSNALPVGLPLPSHVSLTGESDGGYMDMSKDESVDYVPMLDMKGDVKYADIESSNYMAPYDNYVPSAPERTCRATLINESPVLSYMDLVGFSYQVANGMEFLASKNCVHRDLAARNVLICEGKLVKICDFGLARDIMRDSNYISKGSTFLPLKWMAPESIFNSLYTTLSDVWSFGILLWEIFTLGGTPYPELPMNEQFYNAIKRGYRMAQPAHASDEIYEIMQKCWEEKFEIRPPFSQLVLLLERLLGEGYKKKYQQVDEEFLRSDHPAILRSQARLPGFHGLRSPLDTSSVLYTAVQPNEGDNDYIIPLPDPKPEVADEGPLEGSPSLASSTLNEVNTSSTISCDSPLEPQDEPEPEPQLELQVEPEPELEQLPDSGCPAPRAEAEDSFL DIP protein P09619 original sequence version: 176 DIP protein P09619 original sequence version: 185 DIP protein P09619 original sequence version: 114 DIP protein P09619 original sequence version: 162 DIP protein P09619 original sequence version: 150 DIP protein P09619 original sequence version: 165 DIP protein P09619 original sequence version: 161 038C15E531D6E89D pdgfa-pdgfb 4 6 unspecified role unspecified role neutral component neutral component pdgf/vegf domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range Tag is attached to aa89 (aa 1-88 and 188-end deleted). c-terminal kt3 epitope (tpppepet) tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range Tag is attached to aa187 (aa 1-88 and 188-end deleted). ecoli Escherichia coli (strain K12) 7 unspecified role unspecified role neutral component neutral component pdgf/vegf domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range Tag is attached to aa182 (aa 1-82 and 183-end deleted). ecoli Escherichia coli (strain K12) 15 11 direct interaction direct interaction Fig. 1a, lane 5 pdgfra-pdgfa-4 1 6 unspecified role unspecified role prey prey region 125i radiolabel 125i radiolabel I125 125I 125I I125 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined pdgf/vegf domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain c-terminal kt3 epitope (tpppepet) tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range Tag is attached to aa187 (aa 1-88 and 188-end deleted). n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range Tag is attached to aa89 (aa 1-88 and 188-end deleted). ecoli Escherichia coli (strain K12) 5 unspecified role unspecified role bait bait extracellular domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. 24 20 direct interaction direct interaction Fig. 4a pdgfra-pdgfab 1 5 unspecified role unspecified role bait bait extracellular domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. 7 unspecified role unspecified role prey prey pdgf/vegf domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range Tag is attached to aa182 (aa 1-82 and 183-end deleted). region 125i radiolabel 125i radiolabel I125 125I 125I I125 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 6 unspecified role unspecified role prey prey c-terminal kt3 epitope (tpppepet) tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range Tag is attached to aa187 (aa 1-88 and 188-end deleted). n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range Tag is attached to aa89 (aa 1-88 and 188-end deleted). pdgf/vegf domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region 125i radiolabel 125i radiolabel I125 125I 125I I125 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 35 29 physical association physical association Fig. 4b pdgfra-pdgfb-6 1 5 unspecified role unspecified role bait bait extracellular domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. 7 unspecified role unspecified role prey prey c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range Tag is attached to aa182 (aa 1-82 and 183-end deleted). pdgf/vegf domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region 125i radiolabel 125i radiolabel I125 125I 125I I125 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 42 39 direct interaction direct interaction Fig. 4c pdgfrb-pdgfab 1 8 unspecified role unspecified role bait bait extracellular domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. 7 unspecified role unspecified role prey prey c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range Tag is attached to aa182 (aa 1-82 and 183-end deleted). pdgf/vegf domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region 125i radiolabel 125i radiolabel I125 125I 125I I125 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 6 unspecified role unspecified role prey prey c-terminal kt3 epitope (tpppepet) tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range Tag is attached to aa187 (aa 1-88 and 188-end deleted). n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range Tag is attached to aa89 (aa 1-88 and 188-end deleted). pdgf/vegf domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region 125i radiolabel 125i radiolabel I125 125I 125I I125 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 54 49 physical association physical association Fig. 4d pdgfrb-pdgfb-1 1 7 unspecified role unspecified role prey prey pdgf/vegf domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range Tag is attached to aa182 (aa 1-82 and 183-end deleted). region 125i radiolabel 125i radiolabel I125 125I 125I I125 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 8 unspecified role unspecified role bait bait extracellular domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. 62 58 direct interaction direct interaction Fig. 4e pdgfra-pdgfab-1 2 5 unspecified role unspecified role bait bait extracellular domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. 7 unspecified role unspecified role prey prey pdgf/vegf domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range Tag is attached to aa182 (aa 1-82 and 183-end deleted). ecoli Escherichia coli (strain K12) 6 unspecified role unspecified role prey prey n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range Tag is attached to aa89 (aa 1-88 and 188-end deleted). pdgf/vegf domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain c-terminal kt3 epitope (tpppepet) tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range Tag is attached to aa187 (aa 1-88 and 188-end deleted). pdgf/vegf domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 67 73 physical association physical association Fig. 5a, 8a The only difference between the two figures is that in Fig. 5a detection is with an anti-B chain antibody while in Fig. 8a detection is with an anti-PDGF chain antibody that detects A- and B-chains. pdgfra-pdgfb-7 2 7 unspecified role unspecified role prey prey pdgf/vegf domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range Tag is attached to aa182 (aa 1-82 and 183-end deleted). ecoli Escherichia coli (strain K12) 5 unspecified role unspecified role bait bait extracellular domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. 76 79 physical association physical association Fig. 5a pdgfrb-pdgfab-1 2 7 unspecified role unspecified role prey prey pdgf/vegf domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range Tag is attached to aa182 (aa 1-82 and 183-end deleted). pdgf/vegf domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 6 unspecified role unspecified role prey prey c-terminal kt3 epitope (tpppepet) tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range Tag is attached to aa187 (aa 1-88 and 188-end deleted). n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range Tag is attached to aa89 (aa 1-88 and 188-end deleted). pdgf/vegf domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 8 unspecified role unspecified role bait bait extracellular domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. 90 82 88 84 physical association physical association Fig. 5b pdgfrb-pdgfb-2 2 8 unspecified role unspecified role bait bait extracellular domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. 7 unspecified role unspecified role prey prey pdgf/vegf domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range Tag is attached to aa182 (aa 1-82 and 183-end deleted). ecoli Escherichia coli (strain K12) 93 95 physical association physical association Fig. 5b pdgfra-pdgfb-8 1 7 competitor competitor competitor prey prey P01127:p.Arg108_Arg109del mutation mutation certain certain sequence position certain certain certain sequence position certain RR .. Wed May 02 14:34:02 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg_arg108-109del_del' Tue Jun 30 20:18:02 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg108_Arg109del' pdgf/vegf domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range Tag is attached to aa182 (aa 1-82 and 183-end deleted). ecoli Escherichia coli (strain K12) 7 unspecified role unspecified role prey prey c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range Tag is attached to aa182 (aa 1-82 and 183-end deleted). region 125i radiolabel 125i radiolabel I125 125I 125I I125 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined pdgf/vegf domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 5 unspecified role unspecified role bait bait extracellular domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. 107 105 physical association physical association Fig. 6 pdgfrb-pdgfb-3 1 7 competitor competitor competitor prey prey pdgf/vegf domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain P01127:p.Arg108_Arg109del mutation mutation certain certain sequence position certain certain certain sequence position certain RR .. Wed May 02 18:51:56 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg_arg108-109del_del' Tue Jun 30 19:06:57 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg108_Arg109del' c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range Tag is attached to aa182 (aa 1-82 and 183-end deleted). ecoli Escherichia coli (strain K12) 8 unspecified role unspecified role bait bait extracellular domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. 7 unspecified role unspecified role prey prey pdgf/vegf domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range Tag is attached to aa182 (aa 1-82 and 183-end deleted). region 125i radiolabel 125i radiolabel I125 125I 125I I125 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 116 114 physical association physical association Fig.6 pdgfrb-pdgfab-2 1 6 competitor competitor competitor prey prey c-terminal kt3 epitope (tpppepet) tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range Tag is attached to aa187 (aa 1-88 and 188-end deleted). n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range Tag is attached to aa89 (aa 1-88 and 188-end deleted). pdgf/vegf domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 7 competitor competitor competitor prey prey pdgf/vegf domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain P01127:p.Arg108_Arg109del mutation mutation certain certain sequence position certain certain certain sequence position certain RR .. Wed May 02 20:14:43 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg_arg108-109del_del' Tue Jun 30 18:20:30 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg108_Arg109del' c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range Tag is attached to aa182 (aa 1-82 and 183-end deleted). ecoli Escherichia coli (strain K12) 8 unspecified role unspecified role bait bait extracellular domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. 6 unspecified role unspecified role prey prey pdgf/vegf domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain c-terminal kt3 epitope (tpppepet) tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range Tag is attached to aa187 (aa 1-88 and 188-end deleted). n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range Tag is attached to aa89 (aa 1-88 and 188-end deleted). region 125i radiolabel 125i radiolabel I125 125I 125I I125 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 7 unspecified role unspecified role prey prey c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range Tag is attached to aa182 (aa 1-82 and 183-end deleted). pdgf/vegf domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region 125i radiolabel 125i radiolabel I125 125I 125I I125 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined P01127:p.Arg108_Arg109del mutation mutation certain certain sequence position certain certain certain sequence position certain RR .. Wed May 02 17:46:46 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg_arg108-109del_del' Tue Jun 30 19:26:13 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg108_Arg109del' ecoli Escherichia coli (strain K12) 125 123 131 137 physical association physical association Fig. 8b pdgfa-4 3 6 unspecified role unspecified role neutral component neutral component n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range Tag is attached to aa89 (aa 1-88 and 188-end deleted). c-terminal kt3 epitope (tpppepet) tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range Tag is attached to aa187 (aa 1-88 and 188-end deleted). pdgf/vegf domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) direct interaction direct interaction Fig. 1a pdgfb-3 3 7 unspecified role unspecified role neutral component neutral component pdgf/vegf domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range Tag is attached to aa182 (aa 1-82 and 183-end deleted). ecoli Escherichia coli (strain K12) direct interaction direct interaction Fig. 1a pdgfra-pdgfb-9 1 5 unspecified role unspecified role bait bait extracellular domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. 7 unspecified role unspecified role prey prey c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range Tag is attached to aa182 (aa 1-82 and 183-end deleted). region 125i radiolabel 125i radiolabel I125 125I 125I I125 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined pdgf/vegf domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) direct interaction direct interaction Fig. 6 pdgfrb-pdgfb-4 1 7 unspecified role unspecified role prey prey pdgf/vegf domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range Tag is attached to aa182 (aa 1-82 and 183-end deleted). region 125i radiolabel 125i radiolabel I125 125I 125I I125 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 8 unspecified role unspecified role bait bait extracellular domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. physical association physical association Fig.6 pdgfra-pdgfa-2 1 5 unspecified role unspecified role bait bait extracellular domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. 6 unspecified role unspecified role prey prey pdgf/vegf domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range Tag is attached to aa89 (aa 1-88 and 188-end deleted). c-terminal kt3 epitope (tpppepet) tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range Tag is attached to aa187 (aa 1-88 and 188-end deleted). region 125i radiolabel 125i radiolabel I125 125I 125I I125 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 6 competitor competitor competitor prey prey n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range Tag is attached to aa89 (aa 1-88 and 188-end deleted). c-terminal kt3 epitope (tpppepet) tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range Tag is attached to aa187 (aa 1-88 and 188-end deleted). pdgf/vegf domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) direct interaction direct interaction Fig. 4a pdgfra-pdgfa-2 1 6 competitor competitor competitor prey prey pdgf/vegf domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range Tag is attached to aa89 (aa 1-88 and 188-end deleted). c-terminal kt3 epitope (tpppepet) tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range Tag is attached to aa187 (aa 1-88 and 188-end deleted). ecoli Escherichia coli (strain K12) 7 competitor competitor competitor prey prey c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range Tag is attached to aa182 (aa 1-82 and 183-end deleted). pdgf/vegf domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 5 unspecified role unspecified role bait bait extracellular domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. 7 unspecified role unspecified role prey prey c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range Tag is attached to aa182 (aa 1-82 and 183-end deleted). region 125i radiolabel 125i radiolabel I125 125I 125I I125 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined pdgf/vegf domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 6 unspecified role unspecified role prey prey c-terminal kt3 epitope (tpppepet) tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range Tag is attached to aa187 (aa 1-88 and 188-end deleted). region 125i radiolabel 125i radiolabel I125 125I 125I I125 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined pdgf/vegf domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range Tag is attached to aa89 (aa 1-88 and 188-end deleted). ecoli Escherichia coli (strain K12) physical association physical association Fig. 4b pdgfra-pdgfb-13 1 7 competitor competitor competitor prey prey c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range Tag is attached to aa182 (aa 1-82 and 183-end deleted). pdgf/vegf domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 7 unspecified role unspecified role prey prey c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range Tag is attached to aa182 (aa 1-82 and 183-end deleted). region 125i radiolabel 125i radiolabel I125 125I 125I I125 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined pdgf/vegf domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 5 unspecified role unspecified role bait bait extracellular domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. direct interaction direct interaction Fig. 4c pdgfrb-pdgfa-3 1 7 competitor competitor competitor prey prey pdgf/vegf domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range Tag is attached to aa182 (aa 1-82 and 183-end deleted). ecoli Escherichia coli (strain K12) 8 unspecified role unspecified role bait bait extracellular domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. 6 competitor competitor competitor prey prey pdgf/vegf domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain c-terminal kt3 epitope (tpppepet) tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range Tag is attached to aa187 (aa 1-88 and 188-end deleted). n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range Tag is attached to aa89 (aa 1-88 and 188-end deleted). ecoli Escherichia coli (strain K12) 6 unspecified role unspecified role prey prey region 125i radiolabel 125i radiolabel I125 125I 125I I125 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined c-terminal kt3 epitope (tpppepet) tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range Tag is attached to aa187 (aa 1-88 and 188-end deleted). pdgf/vegf domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range Tag is attached to aa89 (aa 1-88 and 188-end deleted). ecoli Escherichia coli (strain K12) 7 unspecified role unspecified role prey prey pdgf/vegf domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range Tag is attached to aa182 (aa 1-82 and 183-end deleted). region 125i radiolabel 125i radiolabel I125 125I 125I I125 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) physical association physical association Fig. 4d pdgfrb-pdgfb-7 1 7 competitor competitor competitor prey prey pdgf/vegf domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range Tag is attached to aa182 (aa 1-82 and 183-end deleted). ecoli Escherichia coli (strain K12) 8 unspecified role unspecified role bait bait extracellular domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. 7 unspecified role unspecified role prey prey pdgf/vegf domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range Tag is attached to aa182 (aa 1-82 and 183-end deleted). region 125i radiolabel 125i radiolabel I125 125I 125I I125 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) direct interaction direct interaction Fig. 4e pdgfrb-pdgfa-3 1 7 unspecified role unspecified role prey prey P01127:p.Arg108_Arg109del mutation mutation certain certain sequence position certain certain certain sequence position certain RR .. Wed May 02 17:54:33 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg_arg108-109del_del' Tue Jun 30 18:07:27 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg108_Arg109del' region 125i radiolabel 125i radiolabel I125 125I 125I I125 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range Tag is attached to aa182 (aa 1-82 and 183-end deleted). pdgf/vegf domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 6 unspecified role unspecified role prey prey c-terminal kt3 epitope (tpppepet) tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range Tag is attached to aa187 (aa 1-88 and 188-end deleted). n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range Tag is attached to aa89 (aa 1-88 and 188-end deleted). region 125i radiolabel 125i radiolabel I125 125I 125I I125 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined pdgf/vegf domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 8 unspecified role unspecified role bait bait extracellular domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. physical association physical association Fig. 8b