IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Separation of zeta (zeta) and various gamma (gamma) chains of human embryonic hemoglobin Portland I by reverse-phase high-performance liquid chromatography. Separation of zeta (zeta) and various gamma (gamma) chains of human embryonic hemoglobin Portland I by reverse-phase high-performance liquid chromatography. Anal. Biochem. (0003-2697) 1983 imex curation Randhawa Z.I., Jones R.T., Lie-Injo L.E. 10-Mar-2014: Contacted by IntAct-Help. Only protein-protein interactions human-erythrocyte Homo sapiens erythrocyte Erythrocyte, Red blood cell; Corpuscle; RBC electrophoresis electrophoretic mobility-based method electrophoresis predetermined predetermined participant predetermined Randhawa Z.I., Jones R.T., Lie-Injo L.E. HPLC. Haemoglobin was obtained from normal adults and neonates with Hydrops fetalis due to homozygous a-Thalassaemia. Accepted 2014-FEB-18 by MGT Anal. Biochem. (0003-2697) 1983 Only protein-protein interactions imex curation hbg1_human Hemoglobin subunit gamma-1 HBG1 Hemoglobin gamma-1 chain Gamma-1-globin Hemoglobin gamma-A chain Hb F Agamma PRO2979 protein protein human Homo sapiens Human MGHFTEEDKATITSLWGKVNVEDAGGETLGRLLVVYPWTQRFFDSFGNLSSASAIMGNPKVKAHGKKVLTSLGDAIKHLDDLKGTFAQLSELHCDKLHVDPENFKLLGNVLVTVLAIHFGKEFTPEVQASWQKMVTAVASALSSRYH 8FCDC3DA1B416DDE hbb_human Hemoglobin subunit beta Hemoglobin beta chain Beta-globin HBB protein protein human Homo sapiens Human MVHLTPEEKSAVTALWGKVNVDEVGGEALGRLLVVYPWTQRFFESFGDLSTPDAVMGNPKVKAHGKKVLGAFSDGLAHLDNLKGTFATLSELHCDKLHVDPENFRLLGNVLVCVLAHHFGKEFTPPVQAAYQKVVAGVANALAHKYH DIP protein P68871 original sequence version: 133 A31F6D621C6556A1 heme b Heme b small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) hbg2_human Hemoglobin subunit gamma-2 HBG2 Gamma-2-globin Hb F Ggamma Hemoglobin gamma-2 chain Hemoglobin gamma-G chain protein protein human Homo sapiens Human MGHFTEEDKATITSLWGKVNVEDAGGETLGRLLVVYPWTQRFFDSFGNLSSASAIMGNPKVKAHGKKVLTSLGDAIKHLDDLKGTFAQLSELHCDKLHVDPENFKLLGNVLVTVLAIHFGKEFTPEVQASWQKMVTGVASALSSRYH 8FCDC4441B416DDE hba_human Hemoglobin subunit alpha Hemoglobin alpha chain Alpha-globin HBA1 HBA2 protein protein human Homo sapiens Human MVLSPADKTNVKAAWGKVGAHAGEYGAEALERMFLSFPTTKTYFPHFDLSHGSAQVKGHGKKVADALTNAVAHVDDMPNALSALSDLHAHKLRVDPVNFKLLSHCLLVTLAAHLPAEFTPAVHASLDKFLASVSTVLTSKYR DIP protein P69905 original sequence version: 112 DIP protein P69905 original sequence version: 132 15E13666573BBBAE hbaz_human Hemoglobin subunit zeta Hemoglobin zeta chain Zeta-globin HBAZ HBZ HBZ2 protein protein human Homo sapiens Human MSLTKTERTIIVSMWAKISTQADTIGTETLERLFLSHPQTKTYFPHFDLHPGSAQLRAHGSKVVAAVGDAVKSIDDIGGALSKLSELHAYILRVDPVNFKLLSHCLLVTLAARFPADFTAEAHAAWDKFLSVVSSVLTEKYR B62A9B825743A155 hba1-hbb-1 1 3 unspecified role unspecified role neutral component neutral component 4 unspecified role unspecified role neutral component neutral component 6 unspecified role unspecified role neutral component neutral component physical association physical association Fig. 1a hbg1-hbg2 1 2 unspecified role unspecified role neutral component neutral component 4 unspecified role unspecified role neutral component neutral component 5 unspecified role unspecified role neutral component neutral component physical association physical association Fig. 1b, 2b, 2c hbg1-hbz 1 2 unspecified role unspecified role neutral component neutral component 5 unspecified role unspecified role neutral component neutral component 4 unspecified role unspecified role neutral component neutral component 7 unspecified role unspecified role neutral component neutral component physical association physical association Fig. 1c hbg1-hbz-2 1 5 unspecified role unspecified role neutral component neutral component 2 unspecified role unspecified role neutral component neutral component 3 unspecified role unspecified role neutral component neutral component 7 unspecified role unspecified role neutral component neutral component physical association physical association Fig. 3 Amino acid composition for zeta chain was determined by amino acid analysis. HPLC analysis of a non pre-purified sample can be found in Fig. 4.