MINT MINT, Dpt of Biology, University of Rome Tor Vergata MINT http://mint.bio.uniroma2.it/mint url>http://mint.bio.uniroma2.it/mint/ https://mint.bio.uniroma2.it MINT-[0-9]+ https://mint.bio.uniroma2.it/index.php/results-interactions/?id=${ac} The Molecular INTeraction database (MINT) is a relational database designed to store interactions between biological molecules. http://mint.bio.uniroma2.it Structural basis for the binding of the membrane-proximal C-terminal region of chemokine receptor CCR2 with the cytosolic regulator FROUNT Structural basis for the binding of the membrane-proximal C-terminal region of chemokine receptor CCR2 with the cytosolic regulator FROUNT 2014 imex curation terasawa@structbiol.com 20-Oct-2014: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined terasawa@structbiol.com Accepted 2014-SEP-19 by LUANA 2014 imex curation Structural basis for the binding of the membrane-proximal C-terminal region of chemokine receptor CCR2 with the cytosolic regulator FROUNT Structural basis for the binding of the membrane-proximal C-terminal region of chemokine receptor CCR2 with the cytosolic regulator FROUNT 2014 imex curation terasawa@structbiol.com 20-Oct-2014: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro nmr nuclear magnetic resonance NMR NMR nmr predetermined predetermined participant predetermined terasawa@structbiol.com Accepted 2014-SEP-19 by LUANA 2014 imex curation nup85_human Nuclear pore complex protein Nup85 PCNT1 Nucleoporin Nup85 85 kDa nucleoporin Nucleoporin Nup75 Pericentrin-1 FROUNT NUP85 NUP75 protein protein human Homo sapiens Human MEELDGEPTVTLIPGVNSKKNQMYFDWGPGEMLVCETSFNKKEKSEMVPSCPFIYIIRKDVDVYSQILRKLFNESHGIFLGLQRIDEELTGKSRKSQLVRVSKNYRSVIRACMEEMHQVAIAAKDPANGRQFSSQVSILSAMELIWNLCEILFIEVAPAGPLLLHLLDWVRLHVCEVDSLSADVLGSENPSKHDSFWNLVTILVLQGRLDEARQMLSKEADASPASAGICRIMGDLMRTMPILSPGNTQTLTELELKWQHWHEECERYLQDSTFATSPHLESLLKIMLGDEAALLEQKELLSNWYHFLVTRLLYSNPTVKPIDLHYYAQSSLDLFLGGESSPEPLDNILLAAFEFDIHQVIKECSIALSNWWFVAHLTDLLDHCKLLQSHNLYFGSNMREFLLLEYASGLFAHPSLWQLGVDYFDYCPELGRVSLELHIERIPLNTEQKALKVLRICEQRQMTEQVRSICKILAMKAVRNNRLGSALSWSIRAKDAAFATLVSDRFLRDYCERGCFSDLDLIDNLGPAMMLSDRLTFLGKYREFHRMYGEKRFADAASLLLSLMTSRIAPRSFWMTLLTDALPLLEQKQVIFSAEQTYELMRCLEDLTSRRPVHGESDTEQLQDDDIETTKVEMLRLSLARNLARAIIREGSLEGS 1AE2BA17D9CADF3D p41597-2 C-C chemokine receptor type 2 CCR2 CMKBR2 Monocyte chemoattractant protein 1 receptor protein protein human Homo sapiens Human MLSTSRSRFIRNTNESGEEVTTFFDYDYGAPCHKFDVKQIGAQLLPPLYSLVFIFGFVGNMLVVLILINCKKLKCLTDIYLLNLAISDLLFLITLPLWAHSAANEWVFGNAMCKLFTGLYHIGYFGGIFFIILLTIDRYLAIVHAVFALKARTVTFGVVTSVITWLVAVFASVPGIIFTKCQKEDSVYVCGPYFPRGWNNFHTIMRNILGLVLPLLIMVICYSGILKTLLRCRNEKKRHRAVRVIFTIMIVYFLFWTPYNIVILLNTFQEFFGLSNCESTSQLDQATQVTETLGMTHCCINPIIYAFVGEKFRRYLSVFFRKHITKRFCKQCPVFYRETVDGVTSTNTPSTGEQEVSAGL Mutagenesis of Leu-316 to Thr as well as Phe-320 to Asp decrease interaction with NUP85. EA352636D724D470 ccr2-nup85-1 2 CCR2 Pro-C 4 unspecified role unspecified role neutral component neutral component purified purified region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain FROUNT 3 predetermined predetermined participant predetermined predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified direct interaction direct interaction f3a f3c f3e f3g f4c nup85-ccr2-2 1 CCR2 Pro-C 4 unspecified role unspecified role prey prey region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain P41597-2:p.Leu316Thr mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain L T Wed May 02 14:37:05 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu316thr' Tue Jun 30 15:38:38 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu316Thr' FROUNT 3 predetermined predetermined participant predetermined predetermined predetermined participant predetermined unspecified role unspecified role bait bait purified purified direct interaction direct interaction f4h f5a nup85-ccr2-1 1 CCR2 Pro-C 4 unspecified role unspecified role prey prey purified purified region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain P41597-2:p.Phe319Asp mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain F D Wed May 02 20:25:11 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'phe319asp' Tue Jun 30 19:07:08 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe319Asp' FROUNT 3 predetermined predetermined participant predetermined predetermined predetermined participant predetermined unspecified role unspecified role bait bait purified purified direct interaction direct interaction f4h f5a