MINT MINT, Dpt of Biology, University of Rome Tor Vergata MINT http://mint.bio.uniroma2.it/mint url>http://mint.bio.uniroma2.it/mint/ https://mint.bio.uniroma2.it MINT-[0-9]+ https://mint.bio.uniroma2.it/index.php/results-interactions/?id=${ac} The Molecular INTeraction database (MINT) is a relational database designed to store interactions between biological molecules. http://mint.bio.uniroma2.it NLS copy number variation governs efficiency of nuclear import: case study on dUTPases NLS copy number variation governs efficiency of nuclear import: case study on dUTPases 2014 imex curation rona.gergely@ttk.mta.hu, vertessy@mail.bme.hu 20-Oct-2014: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro comig non denat gel comigration in non denaturing gel electrophoresis comig non denat gel weight by comassie molecular weight estimation by coomasie staining weight by comassie rona.gergely@ttk.mta.hu, vertessy@mail.bme.hu Accepted 2014-SEP-19 by LUANA 2014 imex curation NLS copy number variation governs efficiency of nuclear import: case study on dUTPases NLS copy number variation governs efficiency of nuclear import: case study on dUTPases 2014 imex curation rona.gergely@ttk.mta.hu, vertessy@mail.bme.hu 20-Oct-2014: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column weight by comassie molecular weight estimation by coomasie staining weight by comassie rona.gergely@ttk.mta.hu, vertessy@mail.bme.hu Accepted 2014-SEP-19 by LUANA 2014 imex curation NLS copy number variation governs efficiency of nuclear import: case study on dUTPases NLS copy number variation governs efficiency of nuclear import: case study on dUTPases 2014 imex curation rona.gergely@ttk.mta.hu, vertessy@mail.bme.hu 20-Oct-2014: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined rona.gergely@ttk.mta.hu, vertessy@mail.bme.hu Accepted 2014-SEP-19 by LUANA 2014 imex curation b4ltg5_drovi dUTP diphosphatase Dvir\dUTPase Dvir_GJ10455 protein protein drovi Drosophila virilis (Fruit fly) MASPVIDDIQTAKKMKLDKKCVLRYAKIIEHALEPQRGSERAAGFDLRSAYDLIVPARGKAIVKTDLQVQVPEGSYGRVAPRSGLAVKNFIDVGAGVVDEDYRGNLGVVLFNHSDTDFEVKRGDRIAQFICERISIPELEQVDKLDDTVRGESGFGSTGVKELPGDKQQIVTAKTPTDKKCVLRFAKLTEHALEPQRGSEKAAGLDLRSAYDLKVPSRGKAIVKTDLQVQLPEGSYGRVAPRSGLAVKNFIDVGAGVVDEDYRGNLGVVLFNHSDTDFEVKRGDRIAQFICERIFYPELEQEDKLDDTERGEAGFGSTGVKELPGDKQQIVTAKTPTDKKCVLRFAKLTEHALEPQRGSEKAAGLDLRSAYDLIVPAHGKAIVKTDLQVQLPEGSYGRVAPRSGLAVKNFIDVGAGVVDEDYRGNLGVVLFNHSDTDFEVKRGDRIAQFICELIFYPEPKEEDKLDDTERGEAGFGSTGVKELPEAKLAKQQNGNGKTPLGENDATPIST 7CCFAE714403074A ima1_mouse Importin subunit alpha-1 Kpna2 Rch1 Importin alpha P1 Karyopherin subunit alpha-2 Pendulin Pore targeting complex 58 kDa subunit RAG cohort protein 1 SRP1-alpha protein protein mouse Mus musculus Mouse MSTNENANLPAARLNRFKNKGKDSTEMRRRRIEVNVELRKAKKDEQMLKRRNVSSFPDDATSPLQENRNNQGTVNWSVEDIVKGINSNNLESQLQATQAARKLLSREKQPPIDNIIRAGLIPKFVSFLGKTDCSPIQFESAWALTNIASGTSEQTKAVVDGGAIPAFISLLASPHAHISEQAVWALGNIAGDGSAFRDLVIKHGAIDPLLALLAVPDLSTLACGYLRNLTWTLSNLCRNKNPAPPLDAVEQILPTLVRLLHHNDPEVLADSCWAISYLTDGPNERIEMVVKKGVVPQLVKLLGATELPIVTPALRAIGNIVTGTDEQTQKVIDAGALAVFPSLLTNPKTNIQKEATWTMSNITAGRQDQIQQVVNHGLVPFLVGVLSKADFKTQKEAAWAITNYTSGGTVEQIVYLVHCGIIEPLMNLLSAKDTKIIQVILDAISNIFQAAEKLGETEKLSIMIEECGGLDKIEALQRHENESVYKASLNLIEKYFSVEEEEDQNVVPETTSEGFAFQVQDGAPGTFNF DIP protein P52293 original sequence version: 144 19E64AA3E9C3C7B5 dvir_gj10455-kpna2 2 importin-a 5 weight by comassie molecular weight estimation by coomasie staining weight by comassie weight by comassie molecular weight estimation by coomasie staining weight by comassie unspecified role unspecified role neutral component neutral component purified purified range binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ABC dUTPase 4 weight by comassie molecular weight estimation by coomasie staining weight by comassie weight by comassie molecular weight estimation by coomasie staining weight by comassie unspecified role unspecified role neutral component neutral component purified purified direct interaction direct interaction f6a f6b The size-exclusion chromatography profile for the complex of the ABC dUTPase:importin-α markedly differs from that for the AAA dUTPase:importin-α indicating some alterations in the complexes of different oligomers (Figure 6A). These qualitative observations are also apparent when the size exclusion chromatography profiles are investigated on SDS-PAGE of the chromatography fractions (Figure 6B). dvir_gj10455-kpna2-1 2 AAA dUTPase 4 weight by comassie molecular weight estimation by coomasie staining weight by comassie weight by comassie molecular weight estimation by coomasie staining weight by comassie unspecified role unspecified role neutral component neutral component purified purified range binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain importin-a 5 weight by comassie molecular weight estimation by coomasie staining weight by comassie weight by comassie molecular weight estimation by coomasie staining weight by comassie unspecified role unspecified role neutral component neutral component purified purified range binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain direct interaction direct interaction f6a f6b The size-exclusion chromatography profile for the complex of the ABC dUTPase:importin-α markedly differs from that for the AAA dUTPase:importin-α indicating some alterations in the complexes of different oligomers (Figure 6A). These qualitative observations are also apparent when the size exclusion chromatography profiles are investigated on SDS-PAGE of the chromatography fractions (Figure 6B). dvir_gj10455-kpna2-2 1 ABC dUTPase 4 weight by comassie molecular weight estimation by coomasie staining weight by comassie weight by comassie molecular weight estimation by coomasie staining weight by comassie unspecified role unspecified role neutral component neutral component purified purified importin-a 5 weight by comassie molecular weight estimation by coomasie staining weight by comassie weight by comassie molecular weight estimation by coomasie staining weight by comassie unspecified role unspecified role neutral component neutral component purified purified range binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain direct interaction direct interaction f6d In addition, the size exclusion chromatography profile for the AAA dUTPase:importin-α complex is highly asymmetric, hinting at the possibility of formation of multiple species with different molecular masses. This possibility was investigated in native gel experiments. Figure 6C and D shows that multiple oligomeric forms are present in the AAA dUTPase:importin-α mixtures while only one species can be distinguished in the ABC dUTPase:importin-α mixtures. dvir_gj10455-kpna2-3 1 importin-a 5 weight by comassie molecular weight estimation by coomasie staining weight by comassie weight by comassie molecular weight estimation by coomasie staining weight by comassie unspecified role unspecified role neutral component neutral component purified purified range binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain AAA dUTPase 4 weight by comassie molecular weight estimation by coomasie staining weight by comassie weight by comassie molecular weight estimation by coomasie staining weight by comassie unspecified role unspecified role neutral component neutral component purified purified range binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain direct interaction direct interaction f6c In addition, the size exclusion chromatography profile for the AAA dUTPase:importin-α complex is highly asymmetric, hinting at the possibility of formation of multiple species with different molecular masses. This possibility was investigated in native gel experiments. Figure 6C and D shows that multiple oligomeric forms are present in the AAA dUTPase:importin-α mixtures while only one species can be distinguished in the ABC dUTPase:importin-α mixtures. dvir_gj10455-kpna2-4 3 importin-a 5 predetermined predetermined participant predetermined predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified range binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ABC dUTPase 4 predetermined predetermined participant predetermined predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified range required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain direct interaction direct interaction f7 dvir_gj10455-kpna2-5 3 AAA dUTPase 4 predetermined predetermined participant predetermined predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified range required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain range binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain importin-a 5 predetermined predetermined participant predetermined predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified range binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain direct interaction direct interaction f7