MINT MINT, Dpt of Biology, University of Rome Tor Vergata MINT http://mint.bio.uniroma2.it/mint url>http://mint.bio.uniroma2.it/mint/ https://mint.bio.uniroma2.it MINT-[0-9]+ https://mint.bio.uniroma2.it/index.php/results-interactions/?id=${ac} The Molecular INTeraction database (MINT) is a relational database designed to store interactions between biological molecules. http://mint.bio.uniroma2.it The individual N- and C-lobes of calmodulin tether to the Cav1.2 channel and rescue the channel activity from run-down in ventricular myocytes of guinea-pig heart. The individual N- and C-lobes of calmodulin tether to the Cav1.2 channel and rescue the channel activity from run-down in ventricular myocytes of guinea-pig heart. FEBS Lett. (0014-5793) 2014 imex curation Shao D., Zhao M., Xu J., Feng R., Guo F., Hu H., Sun X., Gao Q., He G., Sun W., Wang H., Yu L., Liu S., Zhu Y., Etsuko M., Zhu T., Kameyama M., Hao L. lyhao@mail.cmu.edu.cn 20-Oct-2014: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie lyhao@mail.cmu.edu.cn Accepted 2014-SEP-17 by LUANA 2014 imex curation calm_human Calmodulin CALM1 CALM CAM CAM1 CALM2 CAMB CALM3 CAM3 CAMC CAMIII CAM2 CALML2 protein protein human Homo sapiens Human MADQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGYISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK DIP protein P62158 original sequence version: 97 DIP protein P62158 original sequence version: 144 DIP protein P62158 original sequence version: 146 DIP protein P62158 original sequence version: 73 DIP protein P62158 original sequence version: 102 DIP protein P62158 original sequence version: 98 DIP protein P62158 original sequence version: 96 DIP protein P62158 original sequence version: 111 DIP protein P62158 original sequence version: 131 DIP protein P62158 original sequence version: 136 DIP protein P62158 original sequence version: 145 DIP protein P62158 original sequence version: 154 DIP protein P62158 original sequence version: 148 DIP protein P62158 original sequence version: 116 DIP protein P62158 original sequence version: 120 DIP protein P62158 original sequence version: 130 6B4BC3FCDE10727B cac1c_cavpo Voltage-dependent L-type calcium channel subunit alpha-1C CACNA1C CACH2 CACN2 CACNL1A1 CCHL1A1 Calcium channel, L type, alpha-1 polypeptide, isoform 1, cardiac muscle Voltage-gated calcium channel subunit alpha Cav1.2 protein protein cavpo Cavia porcellus (Guinea pig) MVPLVQPTTPAYRPLPSHLSADTEVRGRGTLVHEAQLNCFYISPGGSNYGSPRPAHANINANAAAGLAPEHIPTPGAALSWQAAIDAGRQAKLMGSAGNTTISTVSSTQRKRQQYGKPKKQSGTTATRPPRALLCLTLKNPIRRACISIVEWKPFEIIILLTIFANCVALAIYIPFPEDDSNATNSNLERVEYLFLIIFTVEAFLKVIAYGLLFHPNAYLRNGWNLLDFIIVVVGLFSAILEQATKADGANALGGKGAGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHTALLVLFVIIIYAIIGLELFMGKMHKTCYNQEGITDVPAEEDPSPCALESGHGRQCQNGTVCKPGWDGPKHGITNFDNFAFAMLTVFQCITMEGWTDVLYWMQDAMGYELPWVYFVSLVIFGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEDEGVDEEKPRNMSMPTSETESVNTENVAGGDIEGENCGARLAHRISKSKFSRYWRRWNRFCRRKCRAAVKSNVFYWLVIFLVFLNTLTIASEHYNQPHWLTEVQDTANKALLALFTAEMLLKMYSLGLQAYFVSLFNRLDCFIVCGGILETILVETKIMSPLGISVLRCVRLLRIFKITRYWNSLSNLVASLLNSVRSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDEMRTRRSTFDNFPQSLLTVFQILTGEDWNSVMYDGIMAYGGPSFPGMLVCIYFIILFICGNYILLNVFLAIAVDNLADAESLTSAQKEEEEEKERKKLARTASPEKKQEVVEKPAVEETKEEKIELKSITADGESPPTTKINMDDLQPNENEDKSPYPNPDAAGEEDEEEPEMPVGPRPRPLSELHLKEKAVPMPEASAFFIFSPNNRFRLQCHRIVNDTIFTNLILFFILLSSISLAAEDPVQHTSFRNHILFYFDIVFTTIFTIEIALKMTAYGAFLHKGSFCRNYFNILDLLVVSVSLISFGIQSSAINVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIVIVTTLLQFMFACIGVQLFKGKLYTCSDSSKQTEAECKGNYITYKDGEVDQPIIQPRSWENSKFDFDNVLAAMMALFTVSTFEGWPELLYRSIDSHTEDKGPIYNYRVEISIFFIIYIIIIAFFMMNIFVGFVIVTFQEQGEQEYKNCELDKNQRQCVEYALKARPLRRYIPKNQHQYKVWYVVNSTYFEYLMFVLILLNTICLAMQHYGQSCLFKIAMNILNMLFTGLFTVEMILKLIAFKPKHYFCDAWNTFDALIVVGSIVDIAITEVNPAEHTQCSPSMNAEENSRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIVMLFFIYAVIGMQVFGKIALNDTTEINRNNNFQTFPQAVLLLFRCATGEAWQDIMLACMPGKKCAPESDPSNSTEGETPCGSSFAVFYFISFYMLCAFLIINLFVAVVMDNFDYLTRDWSILGPHHLDEFKRIWAEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVSMNMPLNSDGTAMFNATLFALVRTALRIKTEGNLEQANEELRAIIKKIWKRTSMKLLDQVVPPAGDDEVTVGKFYATFLIQEYFRKFKKRKEQGLVGKPSQRNALSLQAGLRTLHDIGPEIRRAISGDLTAEEELDKAMKEAVSAASEDDIFGRAGGLFGNHVSYYQSDSRSTFPQTFTTQRPLHINKAGNNQGDTESPSHEKLVDSTFTPSSYSSTGSNANINNANNTALGRFPHPAGYPSTVSTVEGHRPPSSPATWAQEATRKLGAMRCHSRESQIAVVCQEEPSQDKTYDVELNKDAEYCSEPSLLSTEMLSYKDDENRQLTPPEEDKGDTRPSPKKGFLRSASLGRRASFHLECLKRQKNHGGDISQKTVLPLHLVHHQALAVAGLSPLLQRSHSPTAIPRPCATPPATPGSRGWPPKPIPTLRLEGAESCEKLNSSFPSIHCSSWSEEPSPCGGGSSAARRARPVSLMVPSQAGAPGRQFHGSASSLAEAVLISEGLGQFAQDPKFIEVTTQELADACDMTIGEMENAADNILSGGAPQSPNGTLLPFVNCRDPGQDRAGGDEDEGCACALGRGWSEEELADSRVHVRSL 8B212C3D74ED2DC3 calm1-q9z305 1 Cav1.2 3 weight by comassie molecular weight estimation by coomasie staining weight by comassie weight by comassie molecular weight estimation by coomasie staining weight by comassie unspecified role unspecified role bait bait purified purified gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined CaM C-lobe 2 weight by comassie molecular weight estimation by coomasie staining weight by comassie weight by comassie molecular weight estimation by coomasie staining weight by comassie unspecified role unspecified role prey prey purified purified range binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain direct interaction direct interaction f2 t1 To further examine the Ca2+-dependence of C-lobe binding to the channel, we measured the interaction of CT1 with C-lobe34, an apo C-lobe mimic, at [Ca2+] of ≈ free to 2 mM. As shown in Fig. 2C, C-lobe34 was still able to bind to CT1 in a concentration-dependent manner, but the Ca2+-dependence was diminished. The Bmax was less than 1 mol/mol CaM/CT1 even in 2 mM Ca2+ (Fig. 2D). q9z305-calm1 1 Cav1.2 3 weight by comassie molecular weight estimation by coomasie staining weight by comassie weight by comassie molecular weight estimation by coomasie staining weight by comassie unspecified role unspecified role bait bait purified purified gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined CaM N-lobe 2 weight by comassie molecular weight estimation by coomasie staining weight by comassie weight by comassie molecular weight estimation by coomasie staining weight by comassie unspecified role unspecified role prey prey purified purified range binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain direct interaction direct interaction f3 t1 To confirm that N-lobe may bind with CT1 at low Ca2+, we examined the binding of N-lobe12 with CT1. As shown in Fig. 3C, N-lobe12 bound to CT1 in a concentration-dependent manner but not in a Ca2+-dependent manner. The parameters obtained (Table 1) revealed that Kd values had no obvious Ca2+-dependence. The maximal binding was about 0.75 mol/mol N-lobe12/CT1 (Fig. 3D), supporting that N-lobe was capable to bind to the channel at low [Ca2+].