MINT MINT, Dpt of Biology, University of Rome Tor Vergata MINT http://mint.bio.uniroma2.it/mint url>http://mint.bio.uniroma2.it/mint/ https://mint.bio.uniroma2.it MINT-[0-9]+ https://mint.bio.uniroma2.it/index.php/results-interactions/?id=${ac} The Molecular INTeraction database (MINT) is a relational database designed to store interactions between biological molecules. http://mint.bio.uniroma2.it Structural basis for the specific recognition of IL-18 by its alpha receptor. Structural basis for the specific recognition of IL-18 by its alpha receptor. FEBS Lett. (0014-5793) 2014 imex curation Wei H., Wang D., Qian Y., Liu X., Fan S., Yin H.S., Wang X. xinquanwang@mail.tsinghua.edu.cn 20-Oct-2014: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined xinquanwang@mail.tsinghua.edu.cn Accepted 2014-SEP-16 by LUANA 2014 imex curation Structural basis for the specific recognition of IL-18 by its alpha receptor. Structural basis for the specific recognition of IL-18 by its alpha receptor. FEBS Lett. (0014-5793) 2014 imex curation Wei H., Wang D., Qian Y., Liu X., Fan S., Yin H.S., Wang X. xinquanwang@mail.tsinghua.edu.cn 20-Oct-2014: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column weight by comassie molecular weight estimation by coomasie staining weight by comassie xinquanwang@mail.tsinghua.edu.cn Accepted 2014-SEP-16 by LUANA 2014 imex curation il18r_human Interleukin-18 receptor 1 Interleukin-18 receptor alpha IL18R1 IL1RRP CD218 antigen-like family member A CDw218a IL1 receptor-related protein protein protein human Homo sapiens Human MNCRELPLTLWVLISVSTAESCTSRPHITVVEGEPFYLKHCSCSLAHEIETTTKSWYKSSGSQEHVELNPRSSSRIALHDCVLEFWPVELNDTGSYFFQMKNYTQKWKLNVIRRNKHSCFTERQVTSKIVEVKKFFQITCENSYYQTLVNSTSLYKNCKKLLLENNKNPTIKKNAEFEDQGYYSCVHFLHHNGKLFNITKTFNITIVEDRSNIVPVLLGPKLNHVAVELGKNVRLNCSALLNEEDVIYWMFGEENGSDPNIHEEKEMRIMTPEGKWHASKVLRIENIGESNLNVLYNCTVASTGGTDTKSFILVRKADMADIPGHVFTRGMIIAVLILVAVVCLVTVCVIYRVDLVLFYRHLTRRDETLTDGKTYDAFVSYLKECRPENGEEHTFAVEILPRVLEKHFGYKLCIFERDVVPGGAVVDEIHSLIEKSRRLIIVLSKSYMSNEVRYELESGLHEALVERKIKIILIEFTPVTDFTFLPQSLKLLKSHRVLKWKADKSLSYNSRFWKNLLYLMPAKTVKPGRDEPEVLPVLSES 7173DB9C7EA71D32 il18_human Interleukin-18 IL18 IGIF IL1F4 Iboctadekin Interferon gamma-inducing factor Interleukin-1 gamma protein protein human Homo sapiens Human MAAEPVEDNCINFVAMKFIDNTLYFIAEDDENLESDYFGKLESKLSVIRNLNDQVLFIDQGNRPLFEDMTDSDCRDNAPRTIFIISMYKDSQPRGMAVTISVKCEKISTLSCENKIISFKEMNPPDNIKDTKSDIIFFQRSVPGHDNKMQFESSSYEGYFLACEKERDLFKLILKKEDELGDRSIMFTVQNED DIP protein Q14116 original sequence version: 95 323C62C203788D55 il18-il18r1 1 IL-18R alpha 3 predetermined predetermined participant predetermined predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified range binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain IL-18 4 predetermined predetermined participant predetermined predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified range binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain direct interaction direct interaction t1 f1 f3 f4 sf2 The final model in the asymmetric unit contains two IL-18-IL-18Rα complexes (Figure S2A), with residues Tyr1-Asn155 in IL-18 and residues Ser21-Cys43, Thr51-Arg71, Arg75-Leu161, Pro169-GLu253, and Asn260-Asp318 in IL-18Rα. il18-il18r1-1 2 IL-18 4 weight by comassie molecular weight estimation by coomasie staining weight by comassie weight by comassie molecular weight estimation by coomasie staining weight by comassie unspecified role unspecified role neutral component neutral component purified purified range binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain IL-18R alpha 3 weight by comassie molecular weight estimation by coomasie staining weight by comassie weight by comassie molecular weight estimation by coomasie staining weight by comassie unspecified role unspecified role neutral component neutral component purified purified range binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain direct interaction direct interaction sf1b The purified IL-18 (residues Tyr1-Asp157) bound to human IL-18Rα ectodomain (residues Ala19-Arg329), which was expressed and purified from insect cells, to form the IL-18-IL-18Rα complex (Figure S1B).