MINT MINT, Dpt of Biology, University of Rome Tor Vergata MINT http://mint.bio.uniroma2.it/mint url>http://mint.bio.uniroma2.it/mint/ https://mint.bio.uniroma2.it MINT-[0-9]+ https://mint.bio.uniroma2.it/index.php/results-interactions/?id=${ac} The Molecular INTeraction database (MINT) is a relational database designed to store interactions between biological molecules. http://mint.bio.uniroma2.it Targeting Alp7/TACC to the spindle pole body is essential for mitotic spindle assembly in fission yeast. Targeting Alp7/TACC to the spindle pole body is essential for mitotic spindle assembly in fission yeast. FEBS Lett. (0014-5793) 2014 imex curation Tang N.H., Okada N., Fong C.S., Arai K., Sato M., Toda T. takashi.toda@cancer.org.uk 21-Jul-2014: Contacted by IntAct-Help. Only protein-protein interactions schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast fluorescence imaging fluorescence microscopy fluorescence imaging tag fluorescence tag visualisation by fluorescence tag fluorescence takashi.toda@cancer.org.uk Accepted 2014-JUN-04 by LUANA 2014 imex curation Targeting Alp7/TACC to the spindle pole body is essential for mitotic spindle assembly in fission yeast. Targeting Alp7/TACC to the spindle pole body is essential for mitotic spindle assembly in fission yeast. FEBS Lett. (0014-5793) 2014 imex curation Tang N.H., Okada N., Fong C.S., Arai K., Sato M., Toda T. takashi.toda@cancer.org.uk 21-Jul-2014: Contacted by IntAct-Help. Only protein-protein interactions schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot takashi.toda@cancer.org.uk Accepted 2014-JUN-04 by LUANA 2014 imex curation Targeting Alp7/TACC to the spindle pole body is essential for mitotic spindle assembly in fission yeast. Targeting Alp7/TACC to the spindle pole body is essential for mitotic spindle assembly in fission yeast. FEBS Lett. (0014-5793) 2014 imex curation Tang N.H., Okada N., Fong C.S., Arai K., Sato M., Toda T. takashi.toda@cancer.org.uk 21-Jul-2014: Contacted by IntAct-Help. Only protein-protein interactions schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot takashi.toda@cancer.org.uk Accepted 2014-JUN-04 by LUANA 2014 imex curation alp7_schpo Microtubule protein alp7 Altered polarity protein 7 Transforming acidic coiled-coil protein mia1 alp7 mia1 SPAC890.02c protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MSDIVSSSTDYSRRSPSSSSIGTNETDHTGFHEKRQGASSESLIPPAQRSSEESMPAPKLFPKLTSKPNPQLNLKDTLNKRVSDRLQALELNKSFDFSGTPRPMHPISHPLSQHKTPEFKHRKRNVESILTPKNPSLFSSSNAASQRGSLNTAPSNFAYSHSSSLQTSASSRPPVLSNGSFPRQTNTAPLNPPVHLKDNIRNSATPSTSQADIPTQYPINSTQKQQAKYEAEIEGYKAKLAGTYHEISVLQNTIVNVSGQLIAVNDQLQQLRSGKASTSPSTKDTNMRLVEGHNEETLALQRGKYTQEEVDKLIQERMEKVAEDLHAQYSAKHTQKINAFKANYARKYEATIQELQNQIGTAPNAPKISNSNWEEERRALKADNQTLQKQLEKAIQERQDMSDFLNNFKADMAKSDKLLMQQQSQQTGDLETLRLQLQALQEELRVEREERQQLIQMSEDLVIAMDQLNLEQKS 22B4216C196E310C 22B4216C196E310C alp14_schpo Spindle pole body component alp14 Altered polarity protein 14 alp14 mtc1 SPCC895.07 protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MSQDQEEDYSKLPLESRIVHKVWKVRLSAYEECSKSFSLSADGSDNCFELWNNQSELWKSVLTDSNVAAQEAGTAAFVAYCRFSDPSHLLKAREISVLSISEKCLTSPRAGTRENALEALMLLVEADSAAPVIESIIPSLSARSPKVIASNVAAIASLVEQFGAKVIPSKMIIPHISNLFGHADKNVRKEASRLTVNIYRWTGDPLKDLLFKDLRPVQTKELESLFAELPTEPPKQTRFLKSQQPTSEPNVETQVEEQPALENEESEPEPSDDQFDLVEEVDVLPNVDPNLETLMASSKWKDRKEALDKLLPVLSQPKIKDNDFFNLVAILTKSVSKDANIMVVINAAHCIQAMAKGLRSNFSKYASTSINALLERSKEKKANVIESLSSAMDAVLATSSLDDLAELIASFAGNKNPQIKSSCFSLFSRSFSNMTSLPSKFTVDTCAKACVPGVSDTFEPVRSAAAEALGVLMKLVGERAINQYLSPLDDIRKSKIRSFYETATVKAKAPTKKSKVKPSKQEESKVVVPSNAKAVKKSVVPSSPVVPSPRKATNKSLSMDVSKGNAFENGPLLPRPTTRPVSRGLSRGTSSSLQQKVKASTPLNSGALNETVQNLKNMELDDPAPQPAKHSRVDRYEHPKVLEDNDSTISSLESLKRENEELREQLKVEHEENISMQKQLSELKGELNTLRSARKASPIGDRKPAFMRRANTDFLELSTSPSFQRSVREFEPTRPKLYSSIDVNQRSPLASAKTNGNFTFHAELPRSPFSSRANNINPDWTKAIDLAAKLKQKITEMKQTDQRHQGLIH 78B2027F0EC9A939 78B2027F0EC9A939 alp4_schpo Spindle pole body component alp4 Altered polarity protein 4 alp4 SPBC365.15 protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MVRSISSVLAKEESENGSPIPALEEGSVYHVSLKSEGVSPFSDRIQLNYLYDGKTFSDPSNLNIHQQEACLINELLNAFMGMEGVFVHLQDMKASSEFETIIMPPSFYILPGFDLGIKDIASEMLEMGSHYLSITAFIESRSHFEYGFVNHALCAALRKFVMDYVVLIMQCENQSRIDPNFSLQTLRLYTLPTSRSLRQVYLILRDLLLSMEKNASSSDDLGLSNIDDLLEQLNEGNDISHVVNATRSKKKVCKGGQVISFLTESLTKYAGDPVARKILTYLLREASRPYTKMLNEWIHLGLVNDPYDEFMIKIHKGITSMQLDEDYTDEYWEKRYVIREDQVPPQLLDLQNKVLFAGKYLNVVLECRKGVNNLASLNAKDDTQNQLLWPSTFDDDNFTLNIMNAYVYANESLLQLLQSSQSLYAHLYSLKHYFFLDQSDFFTTFLDNAQHELRKPAKYISITKLQSQLDLALRQPGTITATDPHKEYVTVEVNQTSLIDWLMHIVSISGLEEGTSSQGNEVWNESITKQADVGNETRNFESEHNRSTQGTSKVGSDKDINGFETMQLCYKVPFPLSLILSRKAIIRYQLLFRYFLLLRHVEMQLENSWVQHSKNSAWRLNSSNAKIEQWKRNSWLLRTRMLSFVQKIIYYTTSEVIETHWGKFMGELENARTVDNLMQEHIDFLDTCLKECMLTNSRLLKVQSKLLNTCAMFASYTSTFTRSLYLLENSEESFDEGRMDKMEEILRRYEDSFSRHLKSLVNACNYFASTETAALLSLVMKLTG E529CE217FFDA2B2 pcp1_schpo Spindle pole body protein pcp1 pcp1 SPAC6G9.06c protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MSERDFNTQSPKFKDENANSVISQSDFLGKSLKNNNDDYSDFRGSYLNDKSSFQTPLRNGSYQPKGSLEFTPLLQSSNKNSDKYNGSLGDKGSFDPNSYGLSAISKQATQEALSISQGNDSYDVSKLTDLSKNSEIDHTDGELPANAALTLREQEKVLEKVSRENFGLRIKIVCLEKRLESMAPEQIKEAVKDNVELHAERANLQLQLKRTESLLQKSEDKNFKLEEKVDYLSKVNDVEQSQNVKVFTERIRFLENALEKVQREKDSLSTEMEEDKSNKEVDYEYEIRQLQNRLDELSEELDVAQDLLTEKEDEIATLKRQIEEKENSSSAFENEENSSYVHLQEDYAILQAKCDEFADRIQVLTADLEKEKENQIMHESEASIGLTDSMQVHTLQEQLHKANEEIEFLHDQISRMNEEGKNFEDIMLQFRSLEEERDVLESKLQTLEDDNNSLRLMTSSLGNQIESLRTQNREIDEEKNHLRLLASKNSDKALAETNIRLQEVTKELETLRMKNSNDLNEIHDLREENEGLTLKIDSITKEKDRLINELEQRIKSYEVNVSELNGTIDEYRNKLKDKEETYNEVMNAFQYKDNDLRRFHESINKLQDREKELTSNLEKKNLVISSLRETVAMLEKERESIKKYLSGNAKDLDNTNLMEILNDKISVLQRQLTDVKDELDVSEEEREEAIVAGQKLSASFELMSNEKQALELKYSSLKNELINAQNLLDRREEELSELSKKLFEERKIRSGSNDDIEKNKEINVLNSELADKLAQIRHLESDKMELDKLVHHLNRGIEEANIEENAVKKRLCLLMGCDYSSVSILQIVSQIEHFVNQQIQTIRSLKQELRHDFVQFSGKKEQELSRSFEKFGLGTETKHDILAQRNRNVSEKMNDLENAAQKFFSSPDRKNGYLYPSEHTSKIEYLEKTIEDLKLALQDELKNRNLLMDDISSYNKQTTKLQEKIKWLERERSILIDELESYRSNQFNYQNNLVQDKNELEERLKEIQKELEVYNNHFMKQAELMTSNVTDESQLMLKTLREALQSKTNNIDHLSTILERNRKEYKSLLDDYNQLRARYKNLQSNTPQSTQSGQYESEIKGLSKLTKYLQSKCRREHSLRLDLAFSKKFILMQLTGYETCNKINLRMLQKIGISPDPDLSKKHIKLKSLIIVVCSIERMKRMKNEWLKQAQLKQSLQRAAAKAKTANY mint Spindle pole body component that binds calmodulin. Overexpression of pcp1 causes the formation of supernumerary SPB-like structures and disrupts both mitotic spindle assembly and chromosome segregation 70264159ADD42424 alp14-alp7-5 1 Alp7 4 tag fluorescence tag visualisation by fluorescence tag fluorescence tag fluorescence tag visualisation by fluorescence tag fluorescence unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed fixed cell fixed cell Q9URY2:p.[Lys341Ala;Tyr344Ala;Arg346_Tyr348delinsAlaAlaAla] mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain RKY AAA certain certain sequence position certain certain certain sequence position certain Y A certain certain sequence position certain certain certain sequence position certain K A Thu Jul 07 12:15:30 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was lys341ala_tyr344ala_arg346ala_lys347ala_tyr348ala Fri Jul 22 11:04:54 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'lys341ala,tyr344ala,arg346ala,lys347ala,tyr348ala' Wed May 02 14:57:11 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys341ala,tyr344ala,arg_lys_tyr346-348ala_ala_ala' Tue Jun 30 18:06:56 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Lys341Ala;Tyr344Ala;Arg346_Tyr348delinsAlaAlaAla]' tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Alp14 5 tag fluorescence tag visualisation by fluorescence tag fluorescence tag fluorescence tag visualisation by fluorescence tag fluorescence unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed fixed cell fixed cell tag rfp tag red fluorescent protein tag red fluorescent protein RFP red fluorescent protein RFP rfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined colocalization colocalization f2b alp14-alp7-6 1 Alp7 4 tag fluorescence tag visualisation by fluorescence tag fluorescence tag fluorescence tag visualisation by fluorescence tag fluorescence unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed fixed cell fixed cell Q9URY2:p.[Lys341Ala;Tyr344Ala;Arg346_Tyr348delinsAlaAlaAla] mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain RKY AAA certain certain sequence position certain certain certain sequence position certain Y A certain certain sequence position certain certain certain sequence position certain K A Thu Jul 07 12:15:33 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was lys341ala_tyr344ala_arg346ala_lys347ala_tyr348ala Fri Jul 22 11:03:32 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'lys341ala,tyr344ala,arg346ala,lys347ala,tyr348ala' Wed May 02 14:41:54 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys341ala,tyr344ala,arg_lys_tyr346-348ala_ala_ala' Tue Jun 30 20:07:36 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Lys341Ala;Tyr344Ala;Arg346_Tyr348delinsAlaAlaAla]' tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Alp4 6 tag fluorescence tag visualisation by fluorescence tag fluorescence tag fluorescence tag visualisation by fluorescence tag fluorescence unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed fixed cell fixed cell tag fluorescent prot tag fluorescent protein tag fluorescent prot tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined colocalization colocalization f2d alp7-pcp1 1 Alp7 4 tag fluorescence tag visualisation by fluorescence tag fluorescence tag fluorescence tag visualisation by fluorescence tag fluorescence unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed fixed cell fixed cell Q9URY2:p.[Lys341Ala;Tyr344Ala;Arg346_Tyr348delinsAlaAlaAla] mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain Y A certain certain sequence position certain certain certain sequence position certain K A certain certain sequence position certain certain certain sequence position certain RKY AAA Thu Jul 07 12:07:26 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was lys341ala_tyr344ala_arg346ala_lys347ala_tyr348ala Fri Jul 22 11:10:11 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'lys341ala,tyr344ala,arg346ala,lys347ala,tyr348ala' Wed May 02 19:13:01 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys341ala,tyr344ala,arg_lys_tyr346-348ala_ala_ala' Tue Jun 30 16:59:49 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Lys341Ala;Tyr344Ala;Arg346_Tyr348delinsAlaAlaAla]' tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Pcp1 7 tag fluorescence tag visualisation by fluorescence tag fluorescence tag fluorescence tag visualisation by fluorescence tag fluorescence unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed fixed cell fixed cell tag rfp tag red fluorescent protein tag red fluorescent protein RFP red fluorescent protein RFP rfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined colocalization colocalization f2e alp7-pcp1-1 2 Alp7 4 anti tag western anti tag western blot anti tag western western blot western blot Immuno blot Immuno blot unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell Pcp1 7 western blot western blot Immuno blot Immuno blot western blot western blot Immuno blot Immuno blot unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level physical association physical association f4g pcp1-alp7 3 Alp7 4 anti tag western anti tag western blot anti tag western western blot western blot Immuno blot Immuno blot unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell Pcp1 7 western blot western blot Immuno blot Immuno blot western blot western blot Immuno blot Immuno blot unspecified role unspecified role bait bait living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level physical association physical association f4g