IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Bromine is an essential trace element for assembly of collagen IV scaffolds in tissue development and architecture. Bromine is an essential trace element for assembly of collagen IV scaffolds in tissue development and architecture. Cell (0092-8674) 2014 imex curation McCall A.S., Cummings C.F., Bhave G., Vanacore R., Page-McCaw A., Hudson B.G. billy.hudson@vanderbilt.edu 18-Sep-2014: Contacted by IntAct-Help. Only protein-protein interactions mouse-pfhr9 Mouse embryonal carcinoma PFHR9 cells pfhr9 Mouse embryonal carcinoma PFHR9 cells chromatography chromatography technology column chromatography chromatography sequence tag sequence tag identification sequence tag McCall A.S., Cummings C.F., Bhave G., Vanacore R., Page-McCaw A., Hudson B.G. I've only included the mass spec data as this pins it down to a single Col4 protein. I've not curated the gel/comassie staining, though have noted the results in the agonist field. Bovine data not added as no MS. Collagen IV matrix was purified from PFHR9 cells, NC1 domains were resolved by SDS-PAGE, excised, and “in-gel” trypsin digested. The resultant sample was enriched for sulfilimine-crosslinked peptides and analyzed using LC-MS. billy.hudson@vanderbilt.edu Accepted 2014-SEP-02 by MGT Cell (0092-8674) 2014 Only protein-protein interactions imex curation co4a1_mouse Collagen alpha-1(IV) chain Col4a1 protein protein mouse Mus musculus Mouse MGPRLSVWLLLLFAALLLHEERSRAAAKGDCGGSGCGKCDCHGVKGQKGERGLPGLQGVIGFPGMQGPEGPHGPPGQKGDAGEPGLPGTKGTRGPPGAAGYPGNPGLPGIPGQDGPPGPPGIPGCNGTKGERGPLGPPGLPGFSGNPGPPGLPGMKGDPGEILGHVPGTLLKGERGFPGIPGMPGSPGLPGLQGPVGPPGFTGPPGPPGPPGPPGEKGQMGSSFQGPKGDKGEQGVSGPPGVPGQAQVKEKGDFAPTGEKGQKGEPGFPGVPGYGEKGEPGKQGPRGKPGKDGEKGERGSPGIPGDSGYPGLPGRQGPQGEKGEAGLPGPPGTVIGTMPLGEKGDRGYPGAPGLRGEPGPKGFPGTPGQPGPPGFPTPGQAGAPGFPGERGEKGDQGFPGVSLPGPSGRDGAPGPPGPPGPPGQPGHTNGIVECQPGPPGDQGPPGTPGQPGLTGEVGQKGQKGESCLACDTEGLRGPPGPQGPPGEIGFPGQPGAKGDRGLPGRDGLEGLPGPQGSPGLIGQPGAKGEPGEIFFDMRLKGDKGDPGFPGQPGMPGRAGTPGRDGHPGLPGPKGSPGSIGLKGERGPPGGVGFPGSRGDIGPPGPPGVGPIGPVGEKGQAGFPGGPGSPGLPGPKGEAGKVVPLPGPPGAAGLPGSPGFPGPQGDRGFPGTPGRPGIPGEKGAVGQPGIGFPGLPGPKGVDGLPGEIGRPGSPGRPGFNGLPGNPGPQGQKGEPGIGLPGLKGQPGLPGIPGTPGEKGSIGGPGVPGEQGLTGPPGLQGIRGDPGPPGVQGPAGPPGVPGIGPPGAMGPPGGQGPPGSSGPPGIKGEKGFPGFPGLDMPGPKGDKGSQGLPGLTGQSGLPGLPGQQGTPGVPGFPGSKGEMGVMGTPGQPGSPGPAGTPGLPGEKGDHGLPGSSGPRGDPGFKGDKGDVGLPGMPGSMEHVDMGSMKGQKGDQGEKGQIGPTGDKGSRGDPGTPGVPGKDGQAGHPGQPGPKGDPGLSGTPGSPGLPGPKGSVGGMGLPGSPGEKGVPGIPGSQGVPGSPGEKGAKGEKGQSGLPGIGIPGRPGDKGDQGLAGFPGSPGEKGEKGSAGTPGMPGSPGPRGSPGNIGHPGSPGLPGEKGDKGLPGLDGVPGVKGEAGLPGTPGPTGPAGQKGEPGSDGIPGSAGEKGEQGVPGRGFPGFPGSKGDKGSKGEVGFPGLAGSPGIPGVKGEQGFMGPPGPQGQPGLPGTPGHPVEGPKGDRGPQGQPGLPGHPGPMGPPGFPGINGPKGDKGNQGWPGAPGVPGPKGDPGFQGMPGIGGSPGITGSKGDMGLPGVPGFQGQKGLPGLQGVKGDQGDQGVPGPKGLQGPPGPPGPYDVIKGEPGLPGPEGPPGLKGLQGPPGPKGQQGVTGSVGLPGPPGVPGFDGAPGQKGETGPFGPPGPRGFPGPPGPDGLPGSMGPPGTPSVDHGFLVTRHSQTTDDPLCPPGTKILYHGYSLLYVQGNERAHGQDLGTAGSCLRKFSTMPFLFCNINNVCNFASRNDYSYWLSTPEPMPMSMAPISGDNIRPFISRCAVCEAPAMVMAVHSQTIQIPQCPNGWSSLWIGYSFVMHTSAGAEGSGQALASPGSCLEEFRSAPFIECHGRGTCNYYANAYSFWLATIERSEMFKKPTPSTLKAGELRTHVSRCQVCMRRT Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen mint EFEEC72AF301E5CF col4a1-col4a1-1 1 2 unspecified role unspecified role neutral component neutral component region identified peptide identified peptide certain certain sequence position certain certain certain sequence position certain met-1533 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain 2 unspecified role unspecified role neutral component neutral component region identified peptide identified peptide certain certain sequence position certain certain certain sequence position certain lys-1651 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain 8 5 9 6 physical association physical association Fig. 1B, Supp. Fig. 1A CHEBI:15858 Bromine ions Peroxidasin