IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Molecular mechanism of action of plant DRM de novo DNA methyltransferases. Molecular mechanism of action of plant DRM de novo DNA methyltransferases. Cell (0092-8674) 2014 imex curation Zhong X., Du J., Hale C.J., Gallego-Bartolome J., Feng S., Vashisht A.A., Chory J., Wohlschlegel J.A., Patel D.J., Jacobsen S.E. pateld@mskcc.org, jacobsen@ucla.edu 18-Sep-2014: Contacted by IntAct-Help. Only protein-protein interactions arath-flower Arabidopsis thaliana flower flower Flower anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Zhong X., Du J., Hale C.J., Gallego-Bartolome J., Feng S., Vashisht A.A., Chory J., Wohlschlegel J.A., Patel D.J., Jacobsen S.E. pateld@mskcc.org, jacobsen@ucla.edu Accepted 2014-SEP-02 by MGT Cell (0092-8674) 2014 Only protein-protein interactions imex curation Molecular mechanism of action of plant DRM de novo DNA methyltransferases. Molecular mechanism of action of plant DRM de novo DNA methyltransferases. Cell (0092-8674) 2014 imex curation Zhong X., Du J., Hale C.J., Gallego-Bartolome J., Feng S., Vashisht A.A., Chory J., Wohlschlegel J.A., Patel D.J., Jacobsen S.E. pateld@mskcc.org, jacobsen@ucla.edu 18-Sep-2014: Contacted by IntAct-Help. Only protein-protein interactions nicbe-leaf Nicotiana benthamiana leaf leaf leaf anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Zhong X., Du J., Hale C.J., Gallego-Bartolome J., Feng S., Vashisht A.A., Chory J., Wohlschlegel J.A., Patel D.J., Jacobsen S.E. pateld@mskcc.org, jacobsen@ucla.edu Accepted 2014-SEP-02 by MGT Cell (0092-8674) 2014 Only protein-protein interactions imex curation Molecular mechanism of action of plant DRM de novo DNA methyltransferases. Molecular mechanism of action of plant DRM de novo DNA methyltransferases. Cell (0092-8674) 2014 imex curation Zhong X., Du J., Hale C.J., Gallego-Bartolome J., Feng S., Vashisht A.A., Chory J., Wohlschlegel J.A., Patel D.J., Jacobsen S.E. pateld@mskcc.org, jacobsen@ucla.edu 18-Sep-2014: Contacted by IntAct-Help. Only protein-protein interactions arath-flower Arabidopsis thaliana flower flower Flower anti tag coip anti tag coimmunoprecipitation anti tag coip ms participant Identification by mass spectrometry ms participant Zhong X., Du J., Hale C.J., Gallego-Bartolome J., Feng S., Vashisht A.A., Chory J., Wohlschlegel J.A., Patel D.J., Jacobsen S.E. pateld@mskcc.org, jacobsen@ucla.edu Accepted 2014-SEP-02 by MGT Cell (0092-8674) 2014 Only protein-protein interactions imex curation Molecular mechanism of action of plant DRM de novo DNA methyltransferases. Molecular mechanism of action of plant DRM de novo DNA methyltransferases. Cell (0092-8674) 2014 imex curation Zhong X., Du J., Hale C.J., Gallego-Bartolome J., Feng S., Vashisht A.A., Chory J., Wohlschlegel J.A., Patel D.J., Jacobsen S.E. pateld@mskcc.org, jacobsen@ucla.edu 18-Sep-2014: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Zhong X., Du J., Hale C.J., Gallego-Bartolome J., Feng S., Vashisht A.A., Chory J., Wohlschlegel J.A., Patel D.J., Jacobsen S.E. pateld@mskcc.org, jacobsen@ucla.edu Accepted 2014-SEP-02 by MGT Cell (0092-8674) 2014 Only protein-protein interactions imex curation hdt3_arath Histone deacetylase HDT3 HDT3 HD2C HD-tuins protein 3 Histone deacetylase 2c F17C15_160 MED24.1 At5g03740 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MEFWGVEVKNGKPLHLDPGLDRLVHISQVALGESKNNVTEPIQLYVTVGSDKLLIGTLSHEKFPQLSTEIVLERNFALSHTWKNGSVFFSGYKVDASDPEPEDLIDDQLEAAGFKAAPKSAAKQVNFQLPNEDVKAKQDDDADGSEEDSSDDDDSENSGDEEEEKVTAESDSEEDDSSDDEEDDSSEEETPKKPEEPKKRSAEPNSSKNPASNKKAKFVTPQKTDSKKPHVHVATPHPSKQAGKNSGGGSTGETSKQQQTPKSAGAFGCKSCTRTFTSEMGLQSHTKAKHSAAA 7B69F3BE8324C1D1 q76ku6_tobac NtDRM1 protein protein tobac Nicotiana tabacum (Common tobacco) Common tobacco MDNNLSGEDNDNIDWDTEDELEIQEIQDTTFSSCMDLRTTGQHTVRCDGEASSSSVPCQSKFIQQFVVMGFPEASIAKAIEQNGEDSDLVLDALLTFKALEDSPEEQPSASPQPCINSDDSSSEYNENLLDDVYEEDSWSSDSDSCRNSAKQCYVKVESSSSSEKEQTLLFLASMGYPAEEASIAMERCGPEASVAELTDFICAAQMARAEDVYLPEDVKPKLNHILNGSGGYKKRKMYNELCKRKKAKAIFEEETIRLPKPMIGFGVPTEPLPAMVRRTLPEQAVGPPFFYYENVALAPKGVWDTISRFLYDIEPEFVDSKYFCAAARKRGYIHNLPVENRFPLFPLAPRTIHEALPLSKKWWPSWDPRTKLNCLQTAIGSAQLTNRIRKAVEDFDGEPPMRVQKFVLDQCRKWNLVWVGRNKVAPLEPDEVEMLLGFPKNHTRGGGISRTDRYKSLGNSFQVDTVAYHLSVLKDLFPGGINVLSLFSGIGGGEVALYRLGIPLNTVVSVEKSEVNRDIVRSWWEQTNQRGNLIHFNDVQQLNGDRLEQLIESFGGFDLVIGGSPCNNLAGSNRVSRDGLEGKESSLFYDYVRILDLVKSIMSRHKH EA64814698B419A2 drm2_arath DNA (cytosine-5)-methyltransferase DRM2 DRM2 Protein DOMAINS REARRANGED METHYLASE 2 T15N1.110/T15N1.120 At5g14620/At5g14630 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MVIWNNDDDDFLEIDNFQSSPRSSPIHAMQCRVENLAGVAVTTSSLSSPTETTDLVQMGFSDEVFATLFDMGFPVEMISRAIKETGPNVETSVIIDTISKYSSDCEAGSSKSKAIDHFLAMGFDEEKVVKAIQEHGEDNMEAIANALLSCPEAKKLPAAVEEEDGIDWSSSDDDTNYTDMLNSDDEKDPNSNENGSKIRSLVKMGFSELEASLAVERCGENVDIAELTDFLCAAQMAREFSEFYTEHEEQKPRHNIKKRRFESKGEPRSSVDDEPIRLPNPMIGFGVPNEPGLITHRSLPELARGPPFFYYENVALTPKGVWETISRHLFEIPPEFVDSKYFCVAARKRGYIHNLPINNRFQIQPPPKYTIHDAFPLSKRWWPEWDKRTKLNCILTCTGSAQLTNRIRVALEPYNEEPEPPKHVQRYVIDQCKKWNLVWVGKNKAAPLEPDEMESILGFPKNHTRGGGMSRTERFKSLGNSFQVDTVAYHLSVLKPIFPHGINVLSLFTGIGGGEVALHRLQIKMKLVVSVEISKVNRNILKDFWEQTNQTGELIEFSDIQHLTNDTIEGLMEKYGGFDLVIGGSPCNNLAGGNRVSRVGLEGDQSSLFFEYCRILEVVRARMRGS DIP protein Q9M548 original sequence version: 60 DIP protein Q9M548 original sequence version: 86 10A38FC068636DB7 h2b6_arath Histone H2B.6 H2B H2BAt HTB9 F16L2.190 At3g45980 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MAPRAEKKPAEKKPAAEKPVEEKSKAEKAPAEKKPKAGKKLPKEAGAGGDKKKKMKKKSVETYKIYIFKVLKQVHPDIGISSKAMGIMNSFINDIFEKLASESSKLARYNKKPTITSREIQTAVRLVLPGELAKHAVSEGTKAVTKFTSS E7693DD90DEB994B msi4_arath WD-40 repeat-containing protein MSI4 MSI4 ACG1 FVE Altered cold-responsive gene 1 protein Nucleosome remodeling factor complex component 4 Protein MULTICOPY SUPPRESSOR OF IRA1 4 F3P11.12 At2g19520 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MESDEAAAVSPQATTPSGGTGASGPKKRGRKPKTKEDSQTPSSQQQSDVKMKESGKKTQQSPSVDEKYSQWKGLVPILYDWLANHNLVWPSLSCRWGPQLEQATYKNRQRLYLSEQTDGSVPNTLVIANCEVVKPRVAAAEHISQFNEEARSPFVKKYKTIIHPGEVNRIRELPQNSKIVATHTDSPDVLIWDVETQPNRHAVLGAANSRPDLILTGHQDNAEFALAMCPTEPFVLSGGKDKSVVLWSIQDHITTIGTDSKSSGSIIKQTGEGTDKNESPTVGPRGVYHGHEDTVEDVAFSPTSAQEFCSVGDDSCLILWDARTGTNPVTKVEKAHDADLHCVDWNPHDDNLILTGSADNTVRLFDRRKLTANGVGSPIYKFEGHKAAVLCVQWSPDKSSVFGSSAEDGLLNIWDYDRVSKKSDRAAKSPAGLFFQHAGHRDKVVDFHWNASDPWTIVSVSDDCETTGGGGTLQIWRMSDLIYRPEEEVVAELEKFKSHVMTCASKP DIP protein O22607 original sequence version: 102 C37F8000F8B33397 ago4_arath Protein argonaute 4 At2g27040 AGO4 OCP11 Protein OVEREXPRESSOR OF CATIONIC PEROXIDASE 11 T20P8.9 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MDSTNGNGADLESANGANGSGVTEALPPPPPVIPPNVEPVRVKTELAEKKGPVRVPMARKGFGTRGQKIPLLTNHFKVDVANLQGHFFHYSVALFYDDGRPVEQKGVGRKILDKVHQTYHSDLDGKEFAYDGEKTLFTYGALPSNKMDFSVVLEEVSATRANGNGSPNGNESPSDGDRKRLRRPNRSKNFRVEISYAAKIPLQALANAMRGQESENSQEAIRVLDIILRQHAARQGCLLVRQSFFHNDPTNCEPVGGNILGCRGFHSSFRTTQGGMSLNMDVTTTMIIKPGPVVDFLIANQNARDPYSIDWSKAKRTLKNLRVKVSPSGQEFKITGLSDKPCREQTFELKKRNPNENGEFETTEVTVADYFRDTRHIDLQYSADLPCINVGKPKRPTYIPLELCALVPLQRYTKALTTFQRSALVEKSRQKPQERMTVLSKALKVSNYDAEPLLRSCGISISSNFTQVEGRVLPAPKLKMGCGSETFPRNGRWNFNNKEFVEPTKIQRWVVVNFSARCNVRQVVDDLIKIGGSKGIEIASPFQVFEEGNQFRRAPPMIRVENMFKDIQSKLPGVPQFILCVLPDKKNSDLYGPWKKKNLTEFGIVTQCMAPTRQPNDQYLTNLLLKINAKLGGLNSMLSVERTPAFTVISKVPTIILGMDVSHGSPGQSDVPSIAAVVSSREWPLISKYRASVRTQPSKAEMIESLVKKNGTEDDGIIKELLVDFYTSSNKRKPEHIIIFRDGVSESQFNQVLNIELDQIIEACKLLDANWNPKFLLLVAQKNHHTKFFQPTSPENVPPGTIIDNKICHPKNNDFYLCAHAGMIGTTRPTHYHVLYDEIGFSADELQELVHSLSYVYQRSTSAISVVAPICYAHLAAAQLGTFMKFEDQSETSSSHGGITAPGPISVAQLPRLKDNVANSMFFC DIP protein Q9ZVD5 original sequence version: 48 C0323FAE9592B147 ago9_arath Protein argonaute 9 AGO9 T10F18.180 At5g21150 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MDSDEPNGSGLPPPPPFVPANLVPEVEPVKKNILLPMARPRGSGSKGQKIPLLTNHFGVKFNKPSGYFFHYSVAINYEDGRPVEAKGIGRKILDKVQETYQSDLGAKYFAYDGEKTLFTVGALPSNKLDFSVVLEEIPSSRNHAGNDTNDADRKRSRRPNQTKKFMVEISYAAKIPMQAIASALQGKETENLQDALRVLDIILRQSAARQGCLLVRQSFFHNDVKNFVPIGGGVSGCRGFHSSFRTTQGGLSLNIDTSTTMIVQPGPVVDFLLANQNKKDPYGMDWNKARRVLKNLRVQITLSNREYKISGLSEHSCKDQLFTWRKPNDKGEFEEVEITVLNYYKERNIEVRYSGDFPCINVGKPKRPTYFPIEFCNLVSLQRYTKSLTNFQRAALVEKSRQKPPERMASLTKGLKDSNYNADPVLQDSGVSIITNFTQVEGRILPTPMLKVGKGENLSPIKGKWNFMRKTLAEPTTVTRWAVVNFSARCDTNTLIRDLIKCGREKGINVEPPFKDVINENPQFRNAPATVRVENMFEQIKSKLPKPPLFLLCILAERKNSDVYGPWKKKNLVDLGIVTQCIAPTRLNDQYLTNVLLKINAKLGGLNSLLAMERSPAMPKVTQVPTIIVGMDVSHGSPGQSDIPSIAAVVSSRQWPLISKYKACVRTQSRKMEMIDNLFKPVNGKDEGMFRELLLDFYYSSENRKPEHIIIFRDGVSESQFNQVLNIELDQMMQACKFLDDTWHPKFTVIVAQKNHHTKFFQSRGPDNVPPGTIIDSQICHPRNFDFYLCAHAGMIGTTRPTHYHVLYDEIGFATDDLQELVHSLSYVYQRSTTAISVVAPVCYAHLAAAQMGTVMKYEELSETSSSHGGITTPGAVPVPPMPQLHNNVSTSMFFC F2043904C1293300 tpr2_arath Topless-related protein 2 TPR2 K20I9.6 At3g16830 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MSSLSRELVFLILQFLDEEKFKESVHKLEQESGFFFNIKYFEEKALAGEWDEVEKYLSGFTKVDDNRYSMKIFFEIRKQKYLEALDRNDRAKAVEILAKDLKVFATFNEELYKEITQLLTLENFRENEQLSKYGDTKSARSIMYTELKKLIEANPLFREKLAFPSFKASRLRTLINQSLNWQHQLCKNPRPNPDIKTLFLDHSCSPSNGARALTPVNLPVAAVARPSNFVPLGVHGGPFQSNPAPAPNANALAGWMANPNPSSSVPSGVVAASPFPMQPSQVNELKHPRAPSNSLGLMDYQSADHEQLMKRLRSAQTSNEVTYPAHSHPPASLDDLPRNVVSTIRQGSVVISMDFHPSHHTLLAVGCSSGEVTLWEVGSREKVVTEPFKIWNMAACSVIFQGSIVKEPSISVTRVAWSPDGNLLGVSFTKHLIHVYAYQGSDLRQHLEIDAHVGCVNDLAFAHPNKQMCVVTCGDDKLIKVWDLSGKKLFTFEGHEAPVYSICPHQKENIQFIFSTALDGKIKAWLYDNVGSRVDYDAPGQWCTTMLYSADGSRLFSCGTSKEGDSFLVEWNESEGALKRTYLGFRKKSAGVVQFDTTRNRFLAVGEDNQIKFWNMDNTNLLTVVEAEGGLPNLPRLRFNKDGNLLAVTTADNGFKILANTDGLRTLRAFEARSFEASKASIDMKVSTSAMASSISPAIGKIEHMDAGSPARPTPIPNGIEAMSRTMEKPRNLDSVDKSKPLELTEIVDPTQCRQVTMPDSKDSVSKVARLLYTNSGVGVLALGSNGVQRLWKWIRNEQNPTGKATASVTPQHWQPNSGLLMANDVPENPEGSVPCIALSKNDSYVMSACGGKVSLFNMMTFKVMTTFMPPPPASTFLAFHPQDNNIIAIGMEDSSIHIYNVRVDEVKTKLKGHQKHITGLAFSTALNILVSSGADAQLFFWTADSWEKKKSSAIQLPPGKAPVGDTRVQFHNDQIQLLVSHETQLAIYDASKMECIHKWVPQEALSSPITSASYSCNSQLVYASFADGNIAVFDAESLRLRCRIAPSAYMPQPTPNSAPIFPQVITAHPQEPNQLAVGLSDGSVKVIEPSELSRRWGVGVAAGSDKAGTENGRPSSSSAANNSSSDQIQR DIP protein Q9LRZ0 original sequence version: 65 mint 7AB804673A5359F4 sinefungin sinefungin (2S,5S)-2,5-diamino-6-[(2R,3S,R,5R)-5-(6-amino-9H-purin-9-yl)-3,4-dihydroxytetrahydrofuran-2-yl]hexanoic acid 6,9-Diamino-1-(6-amino-9H-purin-9-yl)-1,5,6,7,8,9-hexadeoxy-beta-D-ribo-decofuranuronic acid 6,9-Diamino-1-(6-amino-9H-purin-9-yl)-1,5,6,7,8,9-hexadeoxydecofuranuronic acid A 9145 ADENOSYL-ORNITHINE Antibiotic A 9145 sinefungin sinefungina sinefungine sinefunginum (5S)-5-amino-6-[(2R,3S,4R,5R)-5-(6-amino-9H-purin-9-yl)-3,4-dihydroxytetrahydrofuran-2-yl]-L-norleucine small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) LMXOHSDXUQEUSF-YECHIGJVSA-N LMXOHSDXUQEUSF-YECHIGJVSA-N skp1a_arath SKP1-like protein 1A UFO-binding protein 1 SKP1 UIP1 At1g75950 T4O12.17 SKP1A ASK1 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MSAKKIVLKSSDGESFEVEEAVALESQTIAHMVEDDCVDNGVPLPNVTSKILAKVIEYCKRHVEAAASKAEAVEGAATSDDDLKAWDADFMKIDQATLFELILAANYLNIKNLLDLTCQTVADMIKGKTPEEIRTTFNIKNDFTPEEEEEVRRENQWAFE DIP protein Q39255 original sequence version: 115 DIP protein Q39255 original sequence version: 118 DIP protein Q39255 original sequence version: 108 DIP protein Q39255 original sequence version: 88 DIP protein Q39255 original sequence version: 100 56B84C19FD5ABF17 ntdrm1-sinefungin 4 13 unspecified role unspecified role neutral component neutral component 6 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) direct interaction direct interaction 2.8A Rworking = 0.2 Rfree = 0.22 Supp. Table 1 ntdrm1-ntdrm1 2 6 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 6 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Fig. 3C drm2-ago4 3 12 unspecified role unspecified role prey prey 10 unspecified role unspecified role prey prey 5 unspecified role unspecified role prey prey 7 unspecified role unspecified role bait bait biotin ligase recognition peptide tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 11 unspecified role unspecified role prey prey 9 unspecified role unspecified role prey prey 14 unspecified role unspecified role prey prey 8 unspecified role unspecified role prey prey association association Fig. 5A drm2-ago4-1 1 7 unspecified role unspecified role bait bait biotin ligase recognition peptide tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 10 unspecified role unspecified role prey prey physical association physical association Fig. 5B ntdrm1-sinefungin-1 4 6 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain Q76KU6:p.[Glu283Ser;Arg309_Phe310delinsSerSer;Tyr590_Asp591delinsSerSer] mutation mutation certain certain sequence position certain certain certain sequence position certain YD SS certain certain sequence position certain certain certain sequence position certain E S certain certain sequence position certain certain certain sequence position certain RF SS Thu Jul 07 12:18:24 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was glu283ser_arg309ser_phe310ser_tyr590ser_asp591ser Fri Jul 22 10:50:43 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'glu283ser,arg309ser,phe310ser,tyr590ser,asp591ser' Wed May 02 14:54:39 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu283ser,arg_phe309-310ser_ser,tyr_asp590-591ser_ser' Tue Jun 30 18:52:38 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Glu283Ser;Arg309_Phe310delinsSerSer;Tyr590_Asp591delinsSerSer]' ecoli Escherichia coli (strain K12) 13 unspecified role unspecified role neutral component neutral component direct interaction direct interaction 2.5A Rworking = 0.187 Rfree = 0.23 Supp. Table 1