MINT MINT, Dpt of Biology, University of Rome Tor Vergata MINT http://mint.bio.uniroma2.it/mint url>http://mint.bio.uniroma2.it/mint/ https://mint.bio.uniroma2.it MINT-[0-9]+ https://mint.bio.uniroma2.it/index.php/results-interactions/?id=${ac} The Molecular INTeraction database (MINT) is a relational database designed to store interactions between biological molecules. http://mint.bio.uniroma2.it Arginine methylation of the cellular nucleic acid binding protein does not affect its subcellular localization but impedes RNA binding. Arginine methylation of the cellular nucleic acid binding protein does not affect its subcellular localization but impedes RNA binding. FEBS Lett. (0014-5793) 2014 imex curation Wei H.M., Hu H.H., Chang G.Y., Lee Y.J., Li Y.C., Chang H.H., Li C. cli@csmu.edu.tw 11-Jun-2014: Contacted by IntAct-Help. Only protein-protein interactions human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot cli@csmu.edu.tw Accepted 2014-APR-01 by LUANA 2014 imex curation Arginine methylation of the cellular nucleic acid binding protein does not affect its subcellular localization but impedes RNA binding. Arginine methylation of the cellular nucleic acid binding protein does not affect its subcellular localization but impedes RNA binding. FEBS Lett. (0014-5793) 2014 imex curation Wei H.M., Hu H.H., Chang G.Y., Lee Y.J., Li Y.C., Chang H.H., Li C. cli@csmu.edu.tw 11-Jun-2014: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot cli@csmu.edu.tw Accepted 2014-APR-01 by LUANA 2014 imex curation Arginine methylation of the cellular nucleic acid binding protein does not affect its subcellular localization but impedes RNA binding. Arginine methylation of the cellular nucleic acid binding protein does not affect its subcellular localization but impedes RNA binding. FEBS Lett. (0014-5793) 2014 imex curation Wei H.M., Hu H.H., Chang G.Y., Lee Y.J., Li Y.C., Chang H.H., Li C. cli@csmu.edu.tw 11-Jun-2014: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro enzymatic study enzymatic study autoradiography autoradiography cli@csmu.edu.tw Accepted 2014-APR-01 by LUANA 2014 imex curation anm1_human Protein arginine N-methyltransferase 1 PRMT1 Interferon receptor 1-bound protein 4 Histone-arginine N-methyltransferase PRMT1 HMT2 HRMT1L2 IR1B4 protein protein human Homo sapiens Human MAAAEAANCIMENFVATLANGMSLQPPLEEVSCGQAESSEKPNAEDMTSKDYYFDSYAHFGIHEEMLKDEVRTLTYRNSMFHNRHLFKDKVVLDVGSGTGILCMFAAKAGARKVIGIECSSISDYAVKIVKANKLDHVVTIIKGKVEEVELPVEKVDIIISEWMGYCLFYESMLNTVLYARDKWLAPDGLIFPDRATLYVTAIEDRQYKDYKIHWWENVYGFDMSCIKDVAIKEPLVDVVDPKQLVTNACLIKEVDIYTVKVEDLTFTSPFCLQVKRNDYVHALVAYFNIEFTRCHKRTGFSTSPESPYTHWKQTVFYMEDYLTVKTGEEIFGTIGMRPNAKNNRDLDFTIDLDFKGQLCELSCSTDYRMR DIP protein Q99873 original sequence version: 87 DIP protein Q99873 original sequence version: 125 544349801B0E1396 anm1_rat Protein arginine N-methyltransferase 1 Prmt1 Hrmt1l2 Histone-arginine N-methyltransferase PRMT1 protein protein rat Rattus norvegicus (Rat) Rat MAAAEAANCIMEVSCGQAESSEKPNAEDMTSKDYYFDSYAHFGIHEEMLKDEVRTLTYRNSMFHNRHLFKDKVVLDVGSGTGILCMFAAKAGARKVIGIECSSISDYAVKIVKANKLDHVVTIIKGKVEEVELPVEKVDIIISEWMGYCLFYESMLNTVLHARDKWLAPDGLIFPDRATLYVTAIEDRQYKDYKIHWWENVYGFDMSCIKDVAIKEPLVDVVDPKQLVTNACLIKEVDIYTVKVEDLTFTSPFCLQVKRNDYVHALVAYFNIEFTRCHKRTGFSTSPESPYTHWKQTVFYMEDYLTVKTGEEIFGTIGMRPNAKNNRDLDFTIDLDFKGQLCELSCSTDYRMR EDBB0587784C527E cnbp_human CCHC-type zinc finger nucleic acid binding protein Zinc finger protein 9 CNBP ZNF9 RNF163 Cellular nucleic acid-binding protein protein protein human Homo sapiens Human MSSNECFKCGRSGHWARECPTGGGRGRGMRSRGRGGFTSDRGFQFVSSSLPDICYRCGESGHLAKDCDLQEDACYNCGRGGHIAKDCKEPKREREQCCYNCGKPGHLARDCDHADEQKCYSCGEFGHIQKDCTKVKCYRCGETGHVAINCSKTSEVNCYRCGESGHLARECTIEATA 996F398285F52618 cnbp-prmt1 1 CNBP 6 anti tag western anti tag western blot anti tag western western blot western blot Immuno blot Immuno blot unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined PRMT1 4 western blot western blot Immuno blot Immuno blot western blot western blot Immuno blot Immuno blot unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level physical association physical association f1a cnbp-prmt1-1 2 PRMT1 5 anti tag western anti tag western blot anti tag western western blot western blot Immuno blot Immuno blot unspecified role unspecified role bait bait purified purified gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined CNBP 6 western blot western blot Immuno blot Immuno blot western blot western blot Immuno blot Immuno blot unspecified role unspecified role prey prey purified purified his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined direct interaction direct interaction SF2 cnbp-prmt1-2 3 PRMT1 5 weight by comassie molecular weight estimation by coomasie staining weight by comassie autoradiography autoradiography enzyme enzyme unspecified role unspecified role purified purified gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined CNBP 6 weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra autoradiography autoradiography enzyme target enzyme target substrate substrate unspecified role unspecified role purified purified his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined P62633:p.[Arg25Ala;Arg27Ala] mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain R A certain certain sequence position certain certain certain sequence position certain R A Wed May 02 15:08:14 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg25ala,arg27ala' Tue Jun 30 18:46:01 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Arg25Ala;Arg27Ala]' binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain methylation methylation reaction methylation F1B f4b