IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Hsp90-Tau complex reveals molecular basis for specificity in chaperone action. Hsp90-Tau complex reveals molecular basis for specificity in chaperone action. Cell (0092-8674) 2014 imex curation Karagöz G.E., Duarte A.M., Akoury E., Ippel H., Biernat J., Morán Luengo T., Radli M., Didenko T., Nordhues B.A., Veprintsev D.B., Dickey C.A., Mandelkow E., Zweckstetter M., Boelens R., Madl T., Rüdiger S.G. Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Alzheimers - Interactions investigated in the context of Alzheimers disease t.madl@tum.de, s.g.d.rudiger@uu.nl 11-Jun-2014: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro fluorescence spectr classical fluorescence spectroscopy fluorescence spectr predetermined predetermined participant predetermined Karagöz G.E., Duarte A.M., Akoury E., Ippel H., Biernat J., Morán Luengo T., Radli M., Didenko T., Nordhues B.A., Veprintsev D.B., Dickey C.A., Mandelkow E., Zweckstetter M., Boelens R., Madl T., Rüdiger S.G. Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. t.madl@tum.de, s.g.d.rudiger@uu.nl Accepted 2014-MAY-30 by MGT Cell (0092-8674) 2014 Only protein-protein interactions imex curation Hsp90-Tau complex reveals molecular basis for specificity in chaperone action. Hsp90-Tau complex reveals molecular basis for specificity in chaperone action. Cell (0092-8674) 2014 imex curation Karagöz G.E., Duarte A.M., Akoury E., Ippel H., Biernat J., Morán Luengo T., Radli M., Didenko T., Nordhues B.A., Veprintsev D.B., Dickey C.A., Mandelkow E., Zweckstetter M., Boelens R., Madl T., Rüdiger S.G. Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Alzheimers - Interactions investigated in the context of Alzheimers disease t.madl@tum.de, s.g.d.rudiger@uu.nl 11-Jun-2014: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro nmr nuclear magnetic resonance NMR NMR nmr predetermined predetermined participant predetermined Karagöz G.E., Duarte A.M., Akoury E., Ippel H., Biernat J., Morán Luengo T., Radli M., Didenko T., Nordhues B.A., Veprintsev D.B., Dickey C.A., Mandelkow E., Zweckstetter M., Boelens R., Madl T., Rüdiger S.G. Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. t.madl@tum.de, s.g.d.rudiger@uu.nl Accepted 2014-MAY-30 by MGT Cell (0092-8674) 2014 Only protein-protein interactions imex curation Hsp90-Tau complex reveals molecular basis for specificity in chaperone action. Hsp90-Tau complex reveals molecular basis for specificity in chaperone action. Cell (0092-8674) 2014 imex curation Karagöz G.E., Duarte A.M., Akoury E., Ippel H., Biernat J., Morán Luengo T., Radli M., Didenko T., Nordhues B.A., Veprintsev D.B., Dickey C.A., Mandelkow E., Zweckstetter M., Boelens R., Madl T., Rüdiger S.G. Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Alzheimers - Interactions investigated in the context of Alzheimers disease t.madl@tum.de, s.g.d.rudiger@uu.nl 11-Jun-2014: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro saxs x ray scattering saxs predetermined predetermined participant predetermined Karagöz G.E., Duarte A.M., Akoury E., Ippel H., Biernat J., Morán Luengo T., Radli M., Didenko T., Nordhues B.A., Veprintsev D.B., Dickey C.A., Mandelkow E., Zweckstetter M., Boelens R., Madl T., Rüdiger S.G. Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. t.madl@tum.de, s.g.d.rudiger@uu.nl Accepted 2014-MAY-30 by MGT Cell (0092-8674) 2014 Only protein-protein interactions imex curation adenosine 5'-[gamma-thio]triphosphate Adenosine 5'-[gamma-thio]triphosphate small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) p10636-8 Microtubule-associated protein tau Tau-4 MAPT MAPTL MTBT1 TAU Neurofibrillary tangle protein Paired helical filament-tau 2N4R protein protein human Homo sapiens Human MAEPRQEFEVMEDHAGTYGLGDRKDQGGYTMHQDQEGDTDAGLKESPLQTPTEDGSEEPGSETSDAKSTPTAEDVTAPLVDEGAPGKQAAAQPHTEIPEGTTAEEAGIGDTPSLEDEAAGHVTQARMVSKSKDGTGSDDKKAKGADGKTKIATPRGAAPPGQKGQANATRIPAKTPPAPKTPPSSGEPPKSGDRSGYSSPGSPGTPGSRSRTPSLPTPPTREPKKVAVVRTPPKSPSSAKSRLQTAPVPMPDLKNVKSKIGSTENLKHQPGGGKVQIINKKLDLSNVQSKCGSKDNIKHVPGGGSVQIVYKPVDLSKVTSKCGSLGNIHHKPGGGQVEVKSEKLDFKDRVQSKIGSLDNITHVPGGGNKKIETHKLTFRENAKAKTDHGAEIVYKSPVVSGDTSPRHLSNVSSTGSIDMVDSPQLATLADEVSASLAKQGL DIP protein P10636-8 original sequence version: 206 DIP protein P10636-8 original sequence version: 203 DIP protein P10636-8 original sequence version: 166 DIP protein P10636-8 original sequence version: 218 835A8706D847A8CC hs90b_human Heat shock protein HSP 90-beta HSP90AB1 HSP90B HSPC2 HSPCB Heat shock 84 kDa protein protein human Homo sapiens Human MPEEVHHGEEEVETFAFQAEIAQLMSLIINTFYSNKEIFLRELISNASDALDKIRYESLTDPSKLDSGKELKIDIIPNPQERTLTLVDTGIGMTKADLINNLGTIAKSGTKAFMEALQAGADISMIGQFGVGFYSAYLVAEKVVVITKHNDDEQYAWESSAGGSFTVRADHGEPIGRGTKVILHLKEDQTEYLEERRVKEVVKKHSQFIGYPITLYLEKEREKEISDDEAEEEKGEKEEEDKDDEEKPKIEDVGSDEEDDSGKDKKKKTKKIKEKYIDQEELNKTKPIWTRNPDDITQEEYGEFYKSLTNDWEDHLAVKHFSVEGQLEFRALLFIPRRAPFDLFENKKKKNNIKLYVRRVFIMDSCDELIPEYLNFIRGVVDSEDLPLNISREMLQQSKILKVIRKNIVKKCLELFSELAEDKENYKKFYEAFSKNLKLGIHEDSTNRRRLSELLRYHTSQSGDEMTSLSEYVSRMKETQKSIYYITGESKEQVANSAFVERVRKRGFEVVYMTEPIDEYCVQQLKEFDGKSLVSVTKEGLELPEDEEEKKKMEESKAKFENLCKLMKEILDKKVEKVTISNRLVSSPCCIVTSTYGWTANMERIMKAQALRDNSTMGYMMAKKHLEINPDHPIVETLRQKAEADKNDKAVKDLVVLLFETALLSSGFSLEDPQTHSNRIYRMIKLGLGIDEDEVAAEEPNAAVPDEIPPLEGDEDASRMEEVD DIP protein P08238 original sequence version: 91 DIP protein P08238 original sequence version: 149 DIP protein P08238 original sequence version: 165 DIP protein P08238 original sequence version: 167 DIP protein P08238 original sequence version: 210 DIP protein P08238 original sequence version: 187 A93118C214D03810 hsp90ab1-mapt 1 6 unspecified role unspecified role neutral component neutral component n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 5 unspecified role unspecified role neutral component neutral component ecoli Escherichia coli (strain K12) direct interaction direct interaction Fig. 1A The affinity of Hsp90’s for Tau in the ATP state did not differ significantly from the affinity of Hsp90 in the nucleotide-free apo state. hsp90ab1-mapt-1 2 6 unspecified role unspecified role neutral component neutral component n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 5 unspecified role unspecified role neutral component neutral component region 15n label 15n label 15N N15 15N N15 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) direct interaction direct interaction Fig. 1B, 1D hsp90ab1-mapt-2 2 5 unspecified role unspecified role neutral component neutral component ecoli Escherichia coli (strain K12) 6 unspecified role unspecified role neutral component neutral component n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range isoleucine-labelled 13c label 13c label C13 13C C13 13C undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined direct interaction direct interaction Fig. 2, Supp. Fig. 1 adenosine_5___gamma_thio_triphosphate-hsp90ab1 2 4 unspecified role unspecified role neutral component neutral component chemical synthesis Chemical synthesis (Chemical synthesis) 6 unspecified role unspecified role neutral component neutral component isoleucine-labelled 13c label 13c label C13 13C C13 13C undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 5 unspecified role unspecified role neutral component neutral component ecoli Escherichia coli (strain K12) direct interaction direct interaction Fig. 2, Supp. Fig. 1 hsp90ab1-mapt-3 3 6 unspecified role unspecified role neutral component neutral component n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 5 unspecified role unspecified role neutral component neutral component ecoli Escherichia coli (strain K12) direct interaction direct interaction Fig. 3